Structural, transport and microwave properties of 123/sapphire films: Thickness effect

The effect of thickness and growth conditions on the structure and microwave properties has been investigated for the 123/sapphire films. It has been shown that in the conditions of epitaxial growth the Al atoms do not diffuse from substrate into the film and the films with thickness up to 100 nm exhibit the excellent direct current (DC) properties. The increase of thickness of GdBaCuO films causes the formation of extended line-mesh defects and the increase of the surface resistance (R(sub S)). The low value of surface resistance R(sub S)(75 GHz, 77K) = 20 mOhm has been obtained for the two layer YBaCuO/CdBaCuO/sapphire films

Субъективное благополучие студентов психолого-педагогических направлений в период эпидемии COVID-19

Introduction. With the development of humanistic attitudes in society, the importance of issues related to the subjective well-being of the individual increases. It is important not only how successful a person is at work or in educational activities, but also how well he/she feels. The COVID-19 pandemic has significantly changed the learning environment for university students. The study of the factors of subjective well-being of students expands the instrumental capabilities of psychological support during the pandemic. In the case of a repetition of a similar situation, this knowledge will be useful for helping students, and potentially a wider circle of people, to maintain subjective well-being. Aim. The present research aimed to investigate the subjective well-being of students of psychological and pedagogical directions of universities during the pandemic with an emphasis on its emotional component. Methodology and research methods. The research methodology is based on the subjective approach, which considers a student as an active subject, capable of successfully adapting to the changed conditions of an educational activity. In the course of the research, the authors identified the interrelationships of subjective well-being, its semantic markers and self-organisation to expand the possibilities of diagnosing subjective well-being and maintaining it during the periods of extreme social situations, as well as to use semantic markers for self-analysis. To assess subjective well-being, three methods were applied. Self-assessment of satisfaction with one's condition on a 10-point scale was carried out according to the following parameters: sleep, food, communication with family, communication with friends, studies, hobbies, and mood. The authors employed the scale of subjective well-being (by А. Perrudet-Badoux, G. Mendelsohn, J. Chiche, adapted by M. V. Sokolova) and psychosemantic characteristics of the subjective attitude to the situation of distance learning at the university due to the COVID-19 pandemic. To assess the ability of students to organise themselves in the changed learning conditions, the questionnaire of self-organisation of activities (N. T. Feather и M. J. Bond, adapted by E. Yu. Mandrikova) was used. The study involved 406 students between the ages of 18 and 45 years (383 women and 23 men) studying in the areas of psychology and pedagogy at the University of Tyumen and State Kurgan State University. For statistical analysis of the research data, the Mann-Whitney U Test and correlation analysis were used. Results. It was found that the ability to self-organise leads to higher subjective well-being, and this, in turn, stimulates self-organisation. Semantic markers of subjective well-being associated with educational activities during the pandemic, such as comfortable and uncomfortable, interesting and uninteresting, tired and vigorous, were highlighted. The authors revealed objective parameters associated with self-organisation and subjective well-being, namely sleep disturbances. This can lead to the fact that there is not enough daytime and the student works at night, thereby resulting in the disturbance of night sleep, and consequently - poor self-organisation. Scientific novelty._The parameters of subjective well-being and self-organisation of students in a new, extreme social situation, during the COVID-19 pandemic are considered. Practical significance._The data obtained can be used to develop a strategy for teaching students in a pandemic situation and forced self-isolation, as well as to increase subjective well-being in a new social situation. The research results can be applied in psychodiagnostics for a more complete interpretation of the parameters of subjective well-being, as well as for the use of the identified relationships in the programmes of psychological support for students of psychological and pedagogical specialities. Semantic markers of subjective well-being that have received empirical justification can be employed to create a diagnostic scale. © 2021 Russian State Vocational Pedagogical University. All rights reserved.Acknowledgements. The research was funded by the Russian Foundation for Basic Research (RFBR) and Tyumen Region within the research project № 20-413-720004

TRACE ELEMENTS CONTENTS DYNAMICS IN WHOLE BLOOD IN CHILDREN WITH END STAGE RENAL DISEASE RECEIVING DIALYSIS

Homeostasis disorders in kidneys damage is accompanied by the accumulation or reduction of trace elements as a component of Chronic Kidney Disease (CKD) progression. The degree of trace elements misbalanced depends on the stage of CKD. The deep disorders are most common during renal replacement therapy (RRT), especially in children due to anatomic and physiological immaturity. Materials and methods: We investigated the 20 trace elements blood concentrations in 42 children with CKD V D depending on RRT duration used the technique of inductive coupled plasma mass-spectrometry (ICPMS). Results: There is detected significant increase of cobalt (in 3,80 times), cadmium (2,66), lead (2,44) and nickel (7,19) in patients with statistically weighty decrease in vanadium (1,49), chromium (1,62), arsenic (9,45), strontium (2,02), barium (5,29), rubidium (2,69 and zinc (1,46). It was found the increasing levels of aluminum, chromium and zinc in RRT duration enlargement (p<0,05). Conclusions: The course of CKD V D in children is accompanied with substantial and multidirectional changes in trace elements blood levels that characterized by a predominance of accumulation processes versus elimination depending the RRT duration

ЗНАЧЕННЯ ГІПЕРЕОЗИНОФІЛЬНОГО СИНДРОМУ В ДИФЕРЕНЦІЙНІЙ ДІАГНОСТИЦІ ТУБЕРКУЛЬОЗУ ЛЕГЕНЬ

У статті наведено дані про частоту і рівень еозинофілії у хворих, госпіталізованих в стаціонар із підозрою на туберкульоз легень. Збільшення еозинофілів констатовано у 24,9 % пацієнтів, які  потребували дообстеження з метою встановлення діагнозу та призначення адекватного лікування

Results of application of terpenoid-containing drugs in therapy of chronic parodontitis

The therapeutic effect of the drug Antiran containing terpenoid isobornyl acetate on the inflammation in the parodontium in 30 patients with chronic parodontitis of mild severity was investigated. It was used a 30% solution of the drug Antiran as the irrigating liquid with ultrasound scaling and closed curettagein the main group (15 patients), and 0.06% chlorhexidine solution in the comparison group (15 patients). Then, all patients were dialyzed by the solution of the drug Antiran in the parodontal tissues for 20 minutes daily. The dynamics of treatment was assessed by index and functional indicators. It was found that on the 7 th day of treatment, almost all the measurable indicators were differed statistically significantly in the study groups and indicated the best result of treatment in the main group.Исследовано терапевтическое действие препарата Антиран, содержащего терпеноид изоборнилацетат, на воспалительный процесс в пародонте у 30 пациентов с хроническим пародонтитом легкой степени тяжести. Основной группе (15 пациентов) в качестве орошающей жидкости при ультразвуковом скейлинге и закрытом кюретаже применяли 30% раствор препарата Антиран, а группе сравнения (15 пациентов) — 0,06% раствор хлоргексидина. Затем всем пациентам проводили диализ раствора препарата Антиран в ткани пародонта по 20 минут ежедневно. Динамику лечения оценивали по индексным и функциональным показателям. Установлено, что на 7-е сутки лечения почти все измеряемые показатели в исследуемых группах отличались статистически значимо и свидетельствовали о лучшем результате лечения в основной группе

RESEARCH ON NEW BISMUTH-SUBSTITUTED APATITES

The work was carried out within the framework of the Strategic Academic Leadership Program “Priority 2030” (internal project number N-413-99_2023-2024)

Роль галектина-1, -3 в механизмах дисрегуляции Т-клеточного звена иммунного ответа при раке толстого кишечника

The aim of the study was to characterize the features of the subpopulation composition and cytokine-secretory activity of T lymphocytes (Th1, Th17 and Treg) in relation to the concentration of galectin-1 and galectin-3 in the blood of patients with colon cancer.Materials and methods. A total of 26 patients diagnosed with colon cancer were examined. The study material included whole peripheral blood, blood plasma, and supernatants of suspension cultures of mononuclear leukocytes. Lymphocytes isolated from blood were typed by flow cytometry using monoclonal  antibodies. The content of galectin-1 and galectin-3 (in blood plasma) and IFNγ, IL-17A, and TGFβ (in supernatants of mononuclear leukocyte culture in vitro) were determined by enzyme-linked immunosorbent assay. The results obtained were analyzed by statistical methods.Results. In patients with colon cancer, a significant increase in the concentration of galectin-1 and galectin-3 in the blood plasma was found, which was associated with a decrease in the content of CD4+T-bet+ Th1 lymphocytes, CD4+RORC2+ Th17 lymphocytes in the blood and in vitro hyposecretion of IL-17. At the same time, positive correlations were revealed between the concentration of galectin-1 and galectin-3, the content of CD4+FoxP3+ Treg cells in the blood, and the secretion of TGFβ by mononuclear leukocytes in vitro.Conclusion. In colon cancer, increased levels of galectin-1 and galectin-3 in the blood are associated with quantitative deficiency and inhibited secretory activity of effector T lymphocytes and activation of the immunosuppressive functions of regulatory T cells. These results suggest a negative role of galectin 1 and galectin 3 in the mechanisms of regulation of the T cell immune response in colon cancer.Цель исследования – охарактеризовать особенности субпопуляционного состава и цитокин-секреторной активности Т-лимфоцитов (Th1, Th17 и Treg) во взаимосвязи с концентрацией галектина-1 и галектина-3 в крови у больных раком толстого кишечника.Материалы и методы. Обследованы 26 пациентов (14 мужчин и 12 женщин, средний возраст (62,9 ± 6,7) лет) с диагнозом рака толстого кишечника. В группу контроля вошли 17 здоровых доноров (11 мужчин и 6 женщин, средний возраст (58,2 ± 3,1) лет). Материалом исследования служила цельная периферическая кровь, плазма крови и супернатанты суспензионной культуры мононуклеарных лейкоцитов. Выделенные из крови лимфоциты типировали методом проточной лазерной цитофлуориметрии с использованием моноклональных антител. Методом иммуноферментного анализа определяли содержание галектина-1 и галектина-3 (в плазме крови) и IFNγ, IL-17A и TGFβ (в супернатантах культуры мононуклеарных лейкоцитов in vitro). Полученные результаты анализировали статистическими методами.Результаты. У больных раком толстого кишечника установлено значимое увеличение концентрации галектина-1 и галектина-3 в плазме крови, ассоциированное со снижением содержания CD4+T-bet+ Th1- лимфоцитов, CD4+RORC2+ Th17-лимфоцитов в крови и гипосекрецией IL-17 лимфоцитами in vitro. Напротив, выявлена положительная корреляция между концентрацией галектинов 1 и 3, содержанием CD4+FoxP3+Treg клеток в крови и секрецией TGFβ мононуклеарными лейкоцитами in vitro.Заключение. При раке толстого кишечника повышенный уровень галектинов 1 и 3 в крови сопряжен с количественным дефицитом и угнетением секреторной активности эффекторных Т-лимфоцитов, и, напротив, активацией иммуносупрессорных функций  регуляторных Т-клеток. Полученные результаты указывают на негативную роль галектина-1 и галектина-3 в механизмах регуляции Т-клеточного звена иммунного ответа при раке толстого кишечника

Cytotoxic effect of the VVGMCSF-Lact oncolytic virus against 3D cultures of human glioblastoma cells U-87 MG

Background. One of the promising methods of treating tumors is virotherapy, which is based on direct lysis of cancer cells by a virus and a virus-mediated antitumor immune response of the body. For the recombinant vaccinia virus strain VVGMCSF-Lact, producing human GMCSF and the oncotoxic protein lactaptin, cytotoxic and antitumor effects were shown in experiments in vitro and in vivo, respectively, when using adhesive cultures of U-87 MG human glioblastoma cells. 3D cultures are a more relevant tumor model than adhesive models, as they more fully reflect the realistic scenario of cancer development, as well as the response of the tumor to anticancer therapy.The aim. To evaluate the cytotoxic effect of the oncolytic virus VV-GMCSF-Lact against 3D cultures of human glioblastoma U-87 MG.Materials and methods. The following methods were used in the work: cultivation of 3D cell cultures, cytofluorometry, microscopic analysis, virus titration, statistical analysis.Results. U-87 MG cells were transduced with a lentiviral vector carrying the GFP reporter gene. The cytotoxicity of the VV-GMCSF-Lact virus (IC50) against the studied cells was 0.024 PFU/cell. U-87 MG cells were cultured under conditions for the formation of 3D structures. Microscopic analysis showed the oncolytic effect of the virus on the cells of 3D cultures as early as 24 hours after the start of incubation. Flow cytometry showed an increase in the granularity of glioblastoma cells under the action of the virus, which indicates active replication of the virus in the cells. The virus titer was 0.44 PFU/cell.Conclusions. The recombinant VV-GMCSF-Lact virus has a cytotoxic effect on 3D human glioblastoma U-87 MG cell cultures and actively replicates in them. In the future, to test the oncolytic effect of VV-GMCSF-Lact, it is planned to use not only 3D human glioblastoma cultures, but also cerebral organelles obtained in the process of cocultivation of glioblastoma cells and induced human pluripotent cells

GYNOCARE Update: Modern Strategies to Improve Diagnosis and Treatment of Rare Gynecologic Tumors—Current Challenges and Future Directions

More than 50% of all gynecologic tumors can be classified as rare (defined as an incidence of ≤6 per 100,000 women) and usually have a poor prognosis owing to delayed diagnosis and treatment. In contrast to almost all other common solid tumors, the treatment of rare gynecologic tumors (RGT) is often based on expert opinion, retrospective studies, or extrapolation from other tumor sites with similar histology, leading to difficulty in developing guidelines for clinical practice. Currently, gynecologic cancer research, due to distinct scientific and technological challenges, is lagging behind. Moreover, the overall efforts for addressing these challenges are fragmented across different European countries and indeed, worldwide. The GYNOCARE, COST Action CA18117 (European Network for Gynecological Rare Cancer Research) programme aims to address these challenges through the creation of a unique network between key stakeholders covering distinct domains from concept to cure: basic research on RGT, biobanking, bridging with industry, and setting up the legal and regulatory requirements for international innovative clinical trials. On this basis, members of this COST Action, (Working Group 1, “Basic and Translational Research on Rare Gynecological Cancer”) have decided to focus their future efforts on the development of new approaches to improve the diagnosis and treatment of RGT. Here, we provide a brief overview of the current state-of-the-art and describe the goals of this COST Action and its future challenges with the aim to stimulate discussion and promote synergy across scientists engaged in the fight against this rare cancer worldwide