12 research outputs found
Flashing LEDs for microalgal production
Flashing lights are next-generation tools to mitigate light attenuation and increase the photosynthetic efficiency of microalgal cultivation systems illuminated by light-emitting diodes (LEDs). Optimal flashing light conditions depend on the reaction kinetics and properties of the linear electron transfer chain, energy dissipation, and storage mechanisms of a phototroph. In particular, extremely short and intense light flashes potentially mitigate light attenuation in photobioreactors without impairing photosynthesis. Intelligently controlling flashing light units and selecting electronic components can maximize light emission and energy efficiency. We discuss the biological, physical, and technical properties of flashing lights for algal production. We combine recent findings about photosynthetic pathways, self-shading in photobioreactors, and developments in solid-state technology towards the biotechnological application of LEDs to microalgal production.Foundation for Science and Technology (FCT, Portugal) [CCMAR/Multi/04326/2013]Nord UniversityNordland County Government (project Bioteknologi en framtidsrettet naering)INTERREG V-A Espana-Portugal project [0055 ALGARED + 5E]Portuguese Foundation for Science and Technology [SFRH/BD/105541/2014, SFRH/BD/115325/2016]info:eu-repo/semantics/publishedVersio
Chemical profiling of infusions and decoctions of Helichrysum italicum subsp picardii by UHPLC-PDA-MS and in vitro biological activities comparatively with green tea (Camellia sinensis) and rooibos tisane (Aspalathus linearis)
Several medicinal plants are currently used by the food industry as functional additives, for example botanical extracts in herbal drinks. Moreover, the scientific community has recently begun focusing on halophytes as sources of functional beverages. Helichrysum italicum subsp. picardii (everlasting) is an aromatic halophyte common in southern Europe frequently used as spice and in traditional medicine. In this context, this work explored for the first time H. italicum subsp. picardii as a potential source of innovative herbal beverages with potential health promoting properties. For that purpose, infusions and decoctions were prepared from roots, vegetative aerial-organs (stems and leaves) and flowers and evaluated for in vitro antioxidant and anti-diabetic activities. Samples were also assessed for toxicity in different mammalian cell lines and chemically characterized by spectrophotometric methods and ultra-high performance liquid chromatography photo diode array mass-spectrometry (UHPLC-PDA-MS). Results were expressed relating to 'a cup-of-tea' and compared with those obtained with green tea (Camellia sinensis) and rooibos tisane (Aspalathus linearis). Tisanes from the everlasting's above-ground organs, particularly flowers, have high polyphenolic content and several phenolics were identified; the main compounds were chlorogenic and quinic acids, dicaffeoylquinic-acid isomers and gnaphaliin-A. The antioxidant activity of beverages from the everlasting's above-ground organs matched or surpassed that of green tea and rooibos. Its anti-diabetic activity was moderate and toxicity low. Overall, our results suggest that the everlasting is a potential source of innovative and functional herbal beverages. (C) 2017 Elsevier B.V. All rights reserved.national funds through Foundation for Science and Technology (FCT, Portugal) [CCMAR/Multi/04326/2013]FCT [SFRH/BD/94407/2013, SFRH/BD/116604/2016]Research Foundation - Flanders (FWO) [12M8315N]FCT Investigator Programme [IF/00049/2012]info:eu-repo/semantics/publishedVersio
Trends and strategies to enhance triacylglycerols and high-value compounds in microalgae
Microalgae are important sources of triacylglycerols (TAGs) and high-value compounds such as carotenoids and long-chain polyunsaturated fatty acids (LC-PUFAs). TAGs are feedstocks for biofuels or edible oils; carotenoids are used as pigments in the food and feed industries; and LC-PUFAs are beneficial for human health, being also key to the correct development of fish in aquaculture. Current trends in microalgal biotechnology propose the combined production of biofuels with high-value compounds to turn large-scale production of microalgal biomass into an economically feasible venture. As TAGs, carotenoids and LC-PUFAs are lipophilic biomolecules, they not only share biosynthetic precursors and storage sinks, but also their regulation often depends on common environmental stimuli. In general, stressful conditions favor carotenoid and TAGs biosynthesis, whereas the highest accumulation of LC-PUFAs is usually obtained under conditions promoting growth. However, there are known exceptions to these general rules, as a few species are able to accumulate LC-PUFAs under low light, low temperature or long-term stress conditions. Thus, future research on how microalgae sense, transduce and respond to environmental stress will be crucial to understand how the biosynthesis and storage of these lipophilic molecules are regulated. The use of high-throughput methods (e.g. fluorescent activated cell sorting) will provide an excellent opportunity to isolate triple-producers, i.e. microalgae able to accumulate high levels of LC-PUFAs, carotenoids and TAGs simultaneously. Comparative transcriptomics between wild type and tripleproducers could then be used to identify key gene products involved in the regulation of these biomolecules even in microalgal species not amenable to reverse genetics. This combined approach could be a major step towards a better understanding of the microalgal metabolism under different stress conditions. Moreover, the generation of triple-producers would be essential to raise the biomass value in a biorefinery setting and contribute to meet the world's rising demand for food, feed and energy.Foundation for Science and Technology (Portugal) through research programme [CCMAR/Multi/04326/2013]doctoral research grants [SFRH/BD/105541/2014, SFRH/BD/115325/2016]Spanish Ministry of Economy and Competitiveness [AGL2016-74866-C3-02]CEIMARNord University and Nordland County Government project Bioteknologi-en framtidsrettet noeringinfo:eu-repo/semantics/publishedVersio
Microalgae-based unsaponifiable matter as source of natural antioxidants and metal chelators to enhance the value of wet Tetraselmis chuii biomass
The present work aimed to determine the antioxidant, metal chelating and neuroprotective potential of the unsaponifiable matter (UM) of Tetraselmis chuii to be applied to a biorefinery setting. The UM obtained via saponification from crude lipids extracted from microalgal wet biomass showed a radical scavenging activity (RSA) towards the DPPH radical of 90.7 +/- 1.3% and 57.1 +/- 1.2% at a concentration of 10 and 5 mg/ mL, respectively. The UM fraction also displayed metal chelating capacity at a concentration of 5 mg/ mL: 58.5 +/- 1.4% and 50.9 +/- 4.0% for copper and iron, respectively. The chemical characterization of the UM revealed significant levels of total phenolics (TPC, 13.61 mg GAE/g) and carotenoids (2.45 mg/g of beta-carotene, lutein and violaxanthin). Overall, the separation of the UM containing high value metabolites might significantly upgrade the total wet biomass value in a biorefinery, allowing the exploitation of a stream with relevant antioxidant and metal chelating activities.SFRH/BD/105541/2014, IF/00049/2012, CCMAR/Multi/04326/2013info:eu-repo/semantics/publishedVersio
Isolation, identification and biotechnological applications of a novel, robust, free-living Chlorococcum (Oophila) amblystomatis strain isolated from a local pond
Bioprospection of novel autochthonous strains is key to the successful industrial-scale
production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond
inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis
based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel,
free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that
the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this
species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of
this novel isolate, growth performance and biochemical composition were evaluated from the pilot
(2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g m2 day1)
was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3
PBR (26.75 g m2 day1). Chlorococcum amblystomatis displayed high protein content (48%–56% dry
weight (DW)) and moderate levels of total lipids (18%–31% DW), carbohydrates (6%–18% DW) and
ashes (9%–16% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids
(PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls
accounted for 40.24 mg g1 DW, followed by lutein with 5.37 mg g1 DW. Overall, this free-living
Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a
promising growth performance with a high protein content as well as relevant amounts of PUFAs,
chlorophyll, and carotenoids.FCT: UIDB/04326/2020, UIDB/04292/2020. Projects EMBRC.PT ALG-01-0145-FEDER-022121 and BIODATA.PT ALG-01-0145-FEDER-02223.info:eu-repo/semantics/publishedVersio
GUP1 and its close homologue GUP2, encoding multi-membrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae
Many yeast species can utilise glycerol, both as sole carbon source and as an osmolyte. In Saccharomyces cerevisiae, physiological studies have previously shown the presence of an active uptake system driven by electrogenic proton symport. We have used transposon mutagenesis to isolate mutants affected in the transport of glycerol into the cell. Here we present the identification of YGL084c, encoding a multi-membrane-spanning protein, as being essential for proton symport of glycerol into Saccharomyces cerevisiae. The gene is named GUP1 (Glycerol UPtake) and is important for growth on glycerol as carbon and energy source, as well as for osmotic protection by added glycerol, of a strain deficient in glycerol production. Another ORF, YPL189w, presenting a high degree of homology to YGL084c, similarly appears to be involved in active glycerol uptake in salt-containing glucose-based media in strains deficient in glycerol production. Analogously, this gene is named GUP2. To our knowledge, this is the first report on a gene product involved in active transport of glycerol in yeasts. Mutations with the same phenotypes occurred in two other open reading frames of previously unknown function, YDL074c and YPL180w.Comunidade Europeia (CE) - contract BIO4-CT95-0161
Regulation of gene expression and signalling in Saccharomyces cerevisiae under osmotic and salt stress.
Halophytes: Gourmet food with nutritional health benefits?
Although little is known about their nutritional composition, Sarcocornia perennis subsp. perennis, S. perennis subsp. alpini and Salicornia ramosissima (Salicorniaceae) as well as Arthrocnemum macro-stachyum (Amaranthaceae) are consumed in gourmet cuisine. In spite of belonging to different families, these halophytes share morphological and organoleptic characteristics. This work explored the nutritional properties and the antioxidant potential of these species using five integrative methods. All species had a nutritional profile suitable for human consumption with high levels of protein (5.20-13.2 g/100 g dw) and n-3 polyunsaturated fatty acids (FA), particularly or alpha-linolenic acid (19.3-25.9% of total FA), and low concentration of toxic metals (below the limits imposed by the European Commission). These halophytes are also a good source of minerals, particularly sodium (64.1-109 mg/gdw), and S. ramosissima is an excellent source of manganese (204 mu g/g dw). However, due care should be taken not to exceed the legal limits for sodium ingestion. These plants showed also significant antioxidant potential, with high radical scavenging activity (RSA), iron reducing power and total phenolics content (20.5-49.2 mg GAE/g). A. macrostachyum had the highest RSA (lC(50-DPPH) = 0.84 mg/mL; IC50-NO = 0.60 mg/mL), and iron reducing potential (IC50= 0.84 mg/mL) along with high levels of alpha- and gamma-tocopherol (8.74 and 4.71 mg/100 g dw, respectively). (C) 2017 Elsevier Inc. All rights reserved.XtremeBio project - Portuguese Foundation for Science and Technology (FCT) [PTDC/MAR-EST/4346/2012]XtremeGourmet project from the Portuguese National Budget [ALG-01-0247-FEDER-017676]FCT Investigator Programme [IF/00049/2012]FCT project [CCMAR/Multi/04326/2013][FCTFRH/BD/105541/2014]info:eu-repo/semantics/publishedVersio