Multiple origins of the common chameleon in southern Italy

The common chameleon Chamaeleo chamaeleon is a Mediterranean lizard which has been introduced in many islands and its native origin in European countries is debated. Chameleons have been introduced in southern Italy, possibly from the Middle East and Tunisia. We conducted genetic analyses on mitochondrial DNA 16S gene on a larger sample. We observed a multiple origin for the Salento (Apulia, southern Italy) population, with individuals phylogenetically related to populations of North Africa and two areas in the Middle East. Some individuals may have been released before the 1950s and some others in the 1980s, improving the establishment success of this species

Eco-interactions of engineered nanomaterials in the marine environment: Towards an eco-design framework

Marine nano-ecotoxicology has emerged with the purpose to assess the environmental risks associated with engineered nanomaterials (ENMs) among contaminants of emerging concerns entering the marine environment. ENMs’ massive production and integration in everyday life applications, associated with their peculiar physical chemical features, including high biological reactivity, have imposed a pressing need to shed light on risk for humans and the environment. Environmental safety assessment, known as ecosafety, has thus become mandatory with the perspective to develop a more holistic exposure scenario and understand biological effects. Here, we review the current knowledge on behavior and impact of ENMs which end up in the marine environment. A focus on titanium dioxide (n-TiO2) and silver nanoparticles (AgNPs), among metal-based ENMs massively used in commercial products, and polymeric NPs as polystyrene (PS), largely adopted as proxy for nanoplastics, is made. ENMs eco-interactions with chemical molecules including (bio)natural ones and anthropogenic pollutants, forming eco- and bio-coronas and link with their uptake and toxicity in marine organisms are discussed. An ecologically based design strategy (eco-design) is proposed to support the development of new ENMs, including those for environmental applications (e.g., nanoremediation), by balancing their effectiveness with no associated risk for marine organisms and humans

Combination effects of nano-TiO2 and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on biotransformation gene expression in the liver of European sea bass Dicentrarchus labrax

The aim of present study was to investigate the influence of titanium dioxide nanoparticles (nano-TiO2, Aeroxide® P25) on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) dependent biotransformation gene expression in liver of juvenile European sea bass Dicentrarchus labrax. An in vivo 7 day waterborne exposure was performed with nano-TiO2(1mg/L) and 2,3,7,8-TCDD (46pg/L), singly and in combination. The mRNA expression of aryl hydrocarbon receptor repressor (Ahrr), estrogen receptor (erβ2), ABC transport proteins as Abcb1, Abcc1-c2-g2, cytochrome P450 (cyp1a), glutathione-s-transferase (gsta), glutathione reductase (gr) and engulfment and motility (ELMO) domain-containing protein 2 (elmod2) was investigated. Ahrr,erβ2,abcc1 and abcg2 resulted down-regulated with respect to controls in all experimental groups.Co-exposure to nano-TiO2 and 2,3,7,8-TCDD caused a further significant down regulation ofahrr,erβ2,Abcb1andAbcc2compared to single chemical exposure (nano-TiO2or 2,3,7,8-TCDD alone). No effects were observed for 2,3,7,8-TCDD and nano-TiO2 alone in abcb1 gene, while abcc2 was down-regulated by nano-TiO2 alone. Cyp1a,gst and elmod2 genes were up-regulated by 2,3,7,8-TCDD and to a similar extent after co-exposure. Overall the results indicate that nano-TiO2 is unlikely to interfere with 2,3,7,8-TCDD-dependent biotransformation gene expression in the liver of European sea bass, although the effects of co-exposure observed in ABC transport mRNAs might suggest an impact on xenobiotic metabolite disposition and transport in European sea bass liver

Multiple origins of the common chameleon in southern Italy

The common chameleon Chamaeleo chamaeleon is a Mediterranean lizard which has been introduced in many islands and its native origin in European countries is debated. Chameleons have been introduced in southern Italy, possibly from the Middle East and Tunisia. We conducted genetic analyses on mitochondrial DNA 16S gene on a larger sample. We observed a multiple origin for the Salento (Apulia, southern Italy) population, with individuals phylogenetically related to populations of North Africa and two areas in the Middle East. Some individuals may have been released before the 1950s and some others in the 1980s, improving the establishment success of this species

Nano-sized polystyrene affects feeding, behavior and physiology of brine shrimp Artemia franciscana larvae

Nano-sized polymersaspolystyrene(PS)constituteoneofthemainchallengesformarineecosystems, since theycandistributealongthewholewatercolumnaffectingplanktonicspeciesandconsequently disrupting theenergy flowofmarineecosystems.Nowadaysverylittleknowledgeisavailableonthe impact ofnano-sizedplasticsonmarineorganisms.Therefore,thepresentstudyaimstoevaluatethe effects of40nmanioniccarboxylated(PS-COOH)and50nmcationicamino(PS-NH2) polystyrenena- noparticles (PSNPs)onbrineshrimp Artemia franciscana larvae.Nosignsofmortalitywereobservedat 48hofexposureforbothPSNPsatnapliusstagebutseveralsub-lethaleffectswereevident.PS-COOH (5–100 μg/ml) resultedmassivelysequesteredinsidethegutlumenoflarvae(48h)probablylimiting food intake.Someofthemwerelatelyexcretedasfecalpelletsbutnotafullreleasewasobserved. Likewise, PS-NH2 (5–100 mg/ml) accumulatedinlarvae(48h)butalsoadsorbedatthesurfaceofsen- sorial antennulesandappendagesprobablyhamperinglarvaemotility.Inaddition,larvaeexposedtoPS- NH2 undergo multiplemoltingeventsduring48hofexposurecomparedtocontrols.Theactivationofa defense mechanismbasedonaphysiologicalprocessabletoreleasetoxiccationicNPs(PS-NH2) fromthe body canbehypothesized.ThegeneralobservedaccumulationofPSNPswithinthegutduringthe48h of exposureindicatesacontinuousbioavailabilityofnano-sizedPSforplanktonicspeciesaswellasa potentialtransferalongthetrophicweb.Therefore,nano-sizedPSmightbeabletoimpairfooduptake (feeding), behavior(motility)andphysiology(multiplemolting)ofbrineshrimplarvaewithcon- sequencesnotonlyatorganismandpopulationlevelbutontheoverallecosystembasedonthekeyrole of zooplanktononmarinefoodwebs

Time-dependent effects of polystyrene nanoparticles in brine shrimp Artemia franciscana at physiological, biochemical and molecular levels

Micro- (<5 mm) and nanoplastics (<1 \u3bcm) are emerging threats for marine ecosystems worldwide. Brine shrimp Artemia is recognized as a suitable model among planktonic species for studying the impact of polystyrene nanoparticles (PS NPs) through short and long-term bioassays. Our study aims to evaluate the time-dependent effects of cationic amino-modified PS-NH2 (50 nm) in A. franciscana after short- (48 h) and long-term exposure (14 days). For this purpose, nauplii were exposed to a concentration range of PS-NH2 (0.1, 1, 3 and 10 \u3bcg/mL) in natural sea water (NSW), and physiological, biochemical and molecular responses were investigated. Short-term exposure to PS-NH2 caused a decrease in nauplii growth and affected the development in a concentration-dependent manner, long-term exposure impaired the survival, but not the growth and feeding behavior. Oxidative stress was detected after short term exposure as the decrease in the activity of antioxidant enzymes, and was fully evident in the long-term as lipid peroxidation, suggesting an accumulative effect. The decrease in Cholinesterase (ChE) activity observed indicates possible neurotoxic action of PS-NH2. Also, Carboxylesterase (CbE) inhibition by PS-NH2, described for the first time in this study, anticipates potential effects in biotransformation of exogenous and endogenous compounds, being the crustacean juvenile hormone methyl farnesoate (MF) that regulates development and molting, one candidate. Furthermore, short- and long-term exposure to PS-NH2 affect the expression of genes involved in cell protection, development and molting. Overall, our results reveal that low PS-NH2 concentrations induce physiological, biochemical and molecular (changes in gene expression) alterations in Artemia, and point at their potential risk for this model organism, supporting the general concern about nanoplastics occurrences in aquatic environments and their ability to represent an ecological threat for aquatic zooplanktonic species. \ua9 2019 Elsevier B.V

Multi-model inference analysis of toxicological responses and levels of heavy metals in soft tissue of land snail Cornu aspersum caged in proximity to an industrial setting

The use of indicator species has become a standard monitoring approach in environmental risk assessment; however, pollutant bioavailability and its geographical distribution as well as species-sensitiveness could be affected by different factors. The present study investigated the potential use of the land snail Cornu aspersum as bioindicator of industrial air pollution by using cytological and biochemical responses and trace element levels in individuals caged in proximity to an industrial setting. Eleven sites were selected based on wind direction and distance. Snails were placed in holed plastic cages, transplanted to each site and maintained for 30 days fed at libitum and under constant humidity. Several oxidative stress responses including catalase (CAT) and glutathione-s-transferase (GST) activities, levels of malondialdehyde (MDA) and total metallothioneins (MTs) content were determined in snail hepatopancreas while lysosomal membrane stability (LMS) and loss of DNA integrity through micronuclei frequency (MN) were assessed in haemocytes. Trace elements were also analysed in snail whole soft tissues. Multi-model inference used to predict snail’s biological responses in relation to trace elements levels and distance from the industrial setting was able to disentangle the relative contributions of different influencing predictors and could be successfully applied in environmental risk assessment by using land snail as bioindicator species

Amino-modified polystyrene nanoparticles affect signalling pathways of the sea urchin (Paracentrotus lividus) embryos

Polystyrene nanoparticles have been shown to pose serious risk to marine organisms including sea urchin embryos based on their surface properties and consequently behaviour in natural sea water. The aim of this study is to investigate the toxicity pathways of amino polystyrene nanoparticles (PS-NH2, 50 nm) in Paracentrotus lividus embryos in terms of development and signalling at both protein and gene levels. Two sub-lethal concentrations of 3 and 4 \u3bcg/mL of PS-NH2 were used to expose sea urchin embryos in natural sea water (PS-NH2 as aggregates of 143 \ub1 5 nm). At 24 and 48 h post-fertilisation (hpf) embryonic development was monitored and variations in the levels of key proteins involved in stress response and development (Hsp70, Hsp60, MnSOD, Phospho-p38 Mapk) as well as the modulation of target genes (Pl-Hsp70, Pl-Hsp60, Pl-Cytochrome b, Pl-p38 Mapk, Pl-Caspase 8, Pl-Univin) were measured. At 48 hpf various striking teratogenic effects were observed such as the occurrence of cells/masses randomly distributed, severe skeletal defects and delayed development. At 24 hpf a significant up-regulation of Pl-Hsp70, Pl-p38 Mapk, Pl-Univin and Pl-Cas8 genes was found, while at 48 hpf only for Pl-Univin was observed. Protein profile showed different patterns as a significant increase of Hsp70 and Hsp60 only after 48 hpf compared to controls. Conversely, P-p38 Mapk protein significantly increased at 24 hpf and decreased at 48 hpf. Our findings highlight that PS-NH2 are able to disrupt sea urchin embryos development by modulating protein and gene profile providing new understandings into the signalling pathways involved

Aryl hydrocarbon reporter gene bioassay for screening polyhalogenated dibenzo-p-dioxins/furans and dioxin-like polychlorinated biphenyls in hydrochar and sewage sludge

The suitability of the AhR reporter gene bioassays to screen the presence of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (dl-PCBs) in sewage sludge (SL) and related hydmchar (HC) was here investigated. Samples of SL obtained from six WWTPs were processed by hydrothermal carbonization to obtain the resultant HCs and both tested with DR-CALUX (R) bioassay. Levels of PCDD/Fs and dlPCBs were also determined analytically in the same samples by GC-MS/MS. Bioanalytical Toxicity Equivalent values (BEQ) resulted in one order of magnitude higher in HC compared to SL samples and those obtained from the dl-PCBs fraction higher than those from PCDD/Fs. BEQ and TEQw H o values, the latter obtained by GC-MS/MS analysis on the same matrices, were highly correlated showing also a similar trend in the six WWTPs (R-s= 0.8252, p < 0.001; Pearson's R R-p =0.8029, p < 0.01). The suitability of AhR bioassays and in particular of the DR-CALUX (R) to screen the presence and biological activity of legacy organohalogen compounds in both SL and HC matrices was demonstrated for the first time which support their usage for the assessment of potential risks associated with their further environmental applications

Exposure to a nanosilver-enabled consumer product results in similar accumulation and toxicity of silver nanoparticles in the marine mussel Mytilus galloprovincialis

The incorporation of silver nanoparticles (AgNPs) in commercial products is increasing rapidly. The consequent release of AgNPs into domestic and industrial wastewater raises environmental concerns due to their anti-microbial properties and toxicity to non-target aquatic organisms. The aim of the present study was to investigate the effects of nanArgen™ (Nanotek S.A.), a AgNP-enabled consumer product, in the marine bivalve Mytilus galloprovincialis. Two environmentally relevant concentrations of nanArgen™ (1 and 10 μg/L) were tested in vivo for 96 h, and Ag was quantified in mussel soft tissue and natural seawater (NSW). nanArgen™ suspensions were characterized via TEM, SEM, EDS, DLS, and UV–vis optical analysis. Several molecular and biochemical responses were investigated in exposed mussels: lysosomal membrane stability by Neutral Red Retention Time (NRRT) assay; micronucleus (MN) frequency in hemocytes; metallothionein (MT) protein content and gene expression (mt10 and mt20); catalase (CAT) and glutathione-S-transferase (GST) activities; malondialdehyde (MDA) accumulation in digestive glands; and efflux activity of ATP-binding cassette transport proteins (ABC) in gill biopsies. SEM, TEM and DLS analyses confirmed the presence of well-defined AgNPs in nanArgen™ which were roughly spherical with an average particle size of approx. 30 ± 10 nm. DLS analysis revealed the formation of AgNP aggregates in nanArgen™ suspension in NSW (Z-average of 547.80 ± 90.23 nm; PDI of 0.044). A significant concentration-dependent accumulation of Ag was found in mussels’ whole soft tissue in agreement with a concentration-dependent decrease in NRRT and an increase of MN frequency in hemocytes and GST activities in digestive glands. A significant increase in MDA levels and MT via both molecular and biochemical tests, were also observed but only at the highest nanArgen™ concentration (10 μg/L). No changes were observed in CAT activities. ABC efflux activities in gill biopsies showed a significant decrease (p < 0.05) only at the lowest concentration (1 μg/L). On such basis, nanArgen™ is shown to be able to induce toxicity and Ag accumulation in marine mussels similarly to AgNPs and in short-term exposure conditions at environmentally relevant concentrations. AgNP-enabled products, instead of pristine AgNPs, should be the focus of future ecotoxicity studies in order to address any risks associated to their widespread use, disposal and uncontrolled release into the aquatic environment for non target species