Food safety consortium

In recent years, food safety has become an issue of concern for the beef industry, beef processors, and the consuming public. Even though America has the safest and most wholesome food supply in the world, consumers are worried about the safety of the meat they eat. In response to reports of illness from contamination by both microbes and chemicals in the meat supply, the United States Congress, in 1988, authorized and funded a Special Grants Program in the United States Department of Agriculture called the Food Safety Consortium. The members of the Consortium are Kansas State University, the University of Arkansas-Fayetteville and Iowa State University

Food safety consortium

In recent years, food safety has become an issue of concern for the beef industry, beef processors, and the consuming public. Even though America has the safest and most wholesome food supply in the world, consumers are worried about the safety of the meat they eat. In response to reports of illness from contamination by both microbes and chemicals in the meat supply, the United States Congress, in 1988, authorized and funded a Special Grants Program in the United States Department of Agriculture called the Food Safety Consortium. The members of the Consortium are Kansas State University, the University of Arkansas-Fayetteville and Iowa State University

Hydrogen maser development at Laval University

The physical construction of two hydrogen masers is described and results of measurements made on one of the masers are given. These include: cavity Q, thermal time constant, line Q, signal power output, magnetic shielding factor. Preliminary results indicate that the frequency stability will be mainly affected by the thermal of the cavity. The magnetic field and the barometric fluctuations should not affect the maser at the stability level above a few parts in 10 to the 15th power, which is the goal for averaging times of several hours

Coherent spin mixing dynamics in a spin-1 atomic condensate

We study the coherent off-equilibrium spin mixing inside an atomic condensate. Using mean field theory and adopting the single spatial mode approximation (SMA), the condensate spin dynamics is found to be well described by that of a nonrigid pendulum, and displays a variety of periodic oscillations in an external magnetic field. Our results illuminate several recent experimental observations and provide critical insights into the observation of coherent interaction-driven oscillations in a spin-1 condensate.Comment: 6 pages, 5 eps figures, update the discussion of the experimental result

Combined quantum state preparation and laser cooling of a continuous beam of cold atoms

We use two-laser optical pumping on a continuous atomic fountain in order to prepare cold cesium atoms in the same quantum ground state. A first laser excites the F=4 ground state to pump the atoms toward F=3 while a second pi-polarized laser excites the F=3 -> F'=3 transition of the D2 line to produce Zeeman pumping toward m=0. To avoid trap states, we implement the first laser in a 2D optical lattice geometry, thereby creating polarization gradients. This configuration has the advantage of simultaneously producing Sisyphus cooling when the optical lattice laser is tuned between the F=4 -> F'=4 and F=4 -> F'=5 transitions of the D2 line, which is important to remove the heat produced by optical pumping. Detuning the frequency of the second pi-polarized laser reveals the action of a new mechanism improving both laser cooling and state preparation efficiency. A physical interpretation of this mechanism is discussed.Comment: Minor changes according to the recommendations of the referee: - Corrected Fig.1. - Split the graph of Fig.6 for clarity. - Added one reference. - Added two remarks in the conclusion. - Results unchange

Pulsed beams as field probes for precision measurement

We describe a technique for mapping the spatial variation of static electric, static magnetic, and rf magnetic fields using a pulsed atomic or molecular beam. The method is demonstrated using a beam designed to measure the electric dipole moment of the electron. We present maps of the interaction region, showing sensitivity to (i) electric field variation of 1.5 V/cm at 3.3 kV/cm with a spatial resolution of 15 mm; (ii) magnetic field variation of 5 nT with 25 mm resolution; (iii) radio-frequency magnetic field amplitude with 15 mm resolution. This new diagnostic technique is very powerful in the context of high-precision atomic and molecular physics experiments, where pulsed beams have not hitherto found widespread application.Comment: 6 pages, 12 figures. Figures heavily compressed to comply with arxiv's antediluvian file-size polic

Magnetic field imaging with atomic Rb vapor

We demonstrate the possibility of dynamic imaging of magnetic fields using electromagnetically induced transparency in an atomic gas. As an experimental demonstration we employ an atomic Rb gas confined in a glass cell to image the transverse magnetic field created by a long straight wire. In this arrangement, which clearly reveals the essential effect, the field of view is about 2 x 2 mm^2 and the field detection uncertainty is 0.14 mG per 10 um x 10 um image pixel.Comment: 4 pages, 3 figure

Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cells

<p>Abstract</p> <p>Background</p> <p>In the most general sense, studies involving global analysis of gene expression aim to provide a comprehensive catalog of the components involved in the production of recognizable cellular phenotypes. These studies are often limited by the available technologies. One technology, based on microarrays, categorizes gene expression in terms of the abundance of RNA transcripts, and typically employs RNA prepared from whole cells, where cytoplasmic RNA predominates.</p> <p>Results</p> <p>Using microarrays comprising oligonucleotide probes that represent either protein-coding transcripts or microRNAs (miRNA), we have studied global transcript accumulation patterns for the HepG2 (human hepatoma) cell line. Through subdividing the total pool of RNA transcripts into samples from nuclei, the cytoplasm, and whole cells, we determined the degree of correlation of these patterns across these different subcellular locations. The transcript and miRNA abundance patterns for the three RNA fractions were largely similar, but with some exceptions: nuclear RNA samples were enriched with respect to the cytoplasm in transcripts encoding proteins associated with specific nuclear functions, such as the cell cycle, mitosis, and transcription. The cytoplasmic RNA fraction also was enriched, when compared to the nucleus, in transcripts for proteins related to specific nuclear functions, including the cell cycle, DNA replication, and DNA repair. Some transcripts related to the ubiquitin cycle, and transcripts for various membrane proteins were sorted into either the nuclear or cytoplasmic fractions.</p> <p>Conclusion</p> <p>Enrichment or compartmentalization of cell cycle and ubiquitin cycle transcripts within the nucleus may be related to the regulation of their expression, by preventing their translation to proteins. In this way, these cellular functions may be tightly controlled by regulating the release of mRNA from the nucleus and thereby the expression of key rate limiting steps in these pathways. Many miRNA precursors were also enriched in the nuclear samples, with significantly fewer being enriched in the cytoplasm. Studies of mRNA localization will help to clarify the roles RNA processing and transport play in the regulation of cellular function.</p

Non-Destructive Probing of Rabi Oscillations on the Cesium Clock Transition near the Standard Quantum Limit

We report on non-destructive observation of Rabi oscillations on the Cs clock transition. The internal atomic state evolution of a dipole-trapped ensemble of cold atoms is inferred from the phase shift of a probe laser beam as measured using a Mach-Zehnder interferometer. We describe a single color as well as a two-color probing scheme. Using the latter, measurements of the collective pseudo-spin projection of atoms in a superposition of the clock states are performed and the observed spin fluctuations are shown to be close to the standard quantum limit.Comment: 4 pages, 4 figures, accepted for publication in Physical Review Letter

Establishing the precise evolutionary history of a gene improves prediction of disease-causing missense mutations

PURPOSE: Predicting the phenotypic effects of mutations has become an important application in clinical genetic diagnostics. Computational tools evaluate the behavior of the variant over evolutionary time and assume that variations seen during the course of evolution are probably benign in humans. However, current tools do not take into account orthologous/paralogous relationships. Paralogs have dramatically different roles in Mendelian diseases. For example, whereas inactivating mutations in the NPC1 gene cause the neurodegenerative disorder Niemann-Pick C, inactivating mutations in its paralog NPC1L1 are not disease-causing and, moreover, are implicated in protection from coronary heart disease. METHODS: We identified major events in NPC1 evolution and revealed and compared orthologs and paralogs of the human NPC1 gene through phylogenetic and protein sequence analyses. We predicted whether an amino acid substitution affects protein function by reducing the organism’s fitness. RESULTS: Removing the paralogs and distant homologs improved the overall performance of categorizing disease-causing and benign amino acid substitutions. CONCLUSION: The results show that a thorough evolutionary analysis followed by identification of orthologs improves the accuracy in predicting disease-causing missense mutations. We anticipate that this approach will be used as a reference in the interpretation of variants in other genetic diseases as well. Genet Med 18 10, 1029–1036