The impact of various land uses on the microbial and physicochemical quality of surface water bodies in developing countries : prioritisation of water resources management areas

To protect water resources, the WHO recommends assessing land use influence on water quality, taking into consideration residential development and waste disposal amongst others. Thus, we investigated the impact of unconstructed plots, an informal settlement, an urban residential area, and an industrial area on the microbiological and physicochemical quality of two main tributaries within the Klein Jukskei catchment, Johannesburg, South Africa, to identify areas where immediate resource management strategies were needed. Water samples collected from the tributaries’ sources and upstream and downstream from each land use type (Winter and Spring) were analysed for E. coli (indicator organism), using the Colilert® 18 system. Physicochemical parameters (Temperature, pH, dissolved oxygen, electrical conductivity, turbidity and total dissolved solids) were measured using multiparameter instruments. The tributaries’ sources had the lowest E. coli counts (Sandspruit – 0.74; North Ridingspruit – 1.18 log10 MPN/100 mL) during the study. After flowing through the various land uses, mean E. coli counts reached 5.98 (Sandspruit) and 4.85 log10 MPN/100 mL (North Ridingspruit). E. coli values and all physicochemical parameters (but for pH) downstream from most of the land uses did not meet the South African drinking water quality guidelines. The informal settlement had the most negative impact on the microbial and physicochemical quality of the water within the tributaries. Thus, providing informal settlements with appropriate sanitation facilities is likely to prevent pollution of the water bodies. Protection of the sources should also be implemented while industrial wastes need to be monitored for conformity with water quality guidelines before discharge.The authors personally provided all funding for the current research.http://www.elsevier.com/locate/enmm2018-12-30hj2017Geography, Geoinformatics and Meteorolog

Suicide prevention for youth - a mental health awareness program: lessons learned from the Saving and Empowering Young Lives in Europe (SEYLE) intervention study

Background: The Awareness program was designed as a part of the EU-funded Saving and Empowering Young Lives in Europe (SEYLE) intervention study to promote mental health of adolescents in 11 European countries by helping them to develop problem-solving skills and encouraging them to self-recognize the need for help as well as how to help peers in need. Methods: For this descriptive study all coordinators of the SEYLE Awareness program answered an open-ended evaluation questionnaire at the end of the project implementation. Their answers were synthesized and analyzed and are presented here. Results: The results show that the program cultivated peer understanding and support. Adolescents not only learned about mental health by participating in the Awareness program, but the majority of them also greatly enjoyed the experience. Conclusions: Recommendations for enhancing the successes of mental health awareness programs are presented. Help and cooperation from schools, teachers, local politicians and other stakeholders will lead to more efficacious future programs

Challenges and technological approaches for tackling emerging contaminants in drinking and wastewater

In recent decades, emerging contaminants (ECs) have surfaced as one of the key environmental problems threatening ecosystems and public health. Most emerging contaminants are present in low concentrations, and therefore often remain undetected and are also referred to as ‘micropollutants’. Despite this, many ECs raise considerable concerns regarding their impacts on human and environmental health. DEMEAU (Demonstration of promising technologies to address emerging contaminants in water and wastewater), a European Seventh Framework Programme (EU-FP7, 2013-2015) project, aimed to tackle ECs in drinking and wastewater by advancing the uptake of knowledge, prototypes, practices and removal technologies. The project followed a solutions-oriented approach using applied research and demonstration sites, and explored four promising technologies for EC removal and/or degradation: Managed Aquifer Recharge (MAR), Hybrid Ceramic Membrane Filtration (HCMF), Automatic Neural Net Control Systems (ANCS) and Advanced Oxidation Techniques (AOT). Furthermore, Bioassays (BA) were investigated as an effect-based monitoring tool. This article shares new findings for each approach and their potential for widespread integration in the drinking- and wastewater sector. Research results from DEMEAU demonstration sites show that opportunities for synergies among these developments offer the most promising and effective methods for tackling ECs in the water sector

Angiotensin II contributes to podocyte injury by increasing TRPC6 expression via an NFAT-mediated positive feedback signaling pathway

Contains fulltext : 97633.pdf (publisher's version ) (Closed access)The transient receptor potential channel C6 (TRPC6) is a slit diaphragm-associated protein in podocytes involved in regulating glomerular filter function. Gain-of-function mutations in TRPC6 cause hereditary focal segmental glomerulosclerosis (FSGS), and several human acquired proteinuric diseases show increased glomerular TRPC6 expression. Angiotensin II (AngII) is a key contributor to glomerular disease and may regulate TRPC6 expression in nonrenal cells. We demonstrate that AngII regulates TRPC6 mRNA and protein levels in cultured podocytes and that AngII infusion enhances glomerular TRPC6 expression in vivo. In animal models for human FSGS (doxorubicin nephropathy) and increased renin-angiotensin system activity (Ren2 transgenic rats), glomerular TRPC6 expression was increased in an AngII-dependent manner. TRPC6 expression correlated with glomerular damage markers and glomerulosclerosis. We show that the regulation of TRPC6 expression by AngII and doxorubicin requires TRPC6-mediated Ca(2+) influx and the activation of the Ca(2+)-dependent protein phosphatase calcineurin and its substrate nuclear factor of activated T cells (NFAT). Accordingly, calcineurin inhibition by cyclosporine decreased TRPC6 expression and reduced proteinuria in doxorubicin nephropathy, whereas podocyte-specific inducible expression of a constitutively active NFAT mutant increased TRPC6 expression and induced severe proteinuria. Our findings demonstrate that the deleterious effects of AngII on podocytes and its pathogenic role in glomerular disease involve enhanced TRPC6 expression via a calcineurin/NFAT positive feedback signaling pathway