The Interaction Between Signal Control and Traffic Assignment: An Introduction.

Traffic assignment and signal control are generally considered separately. Proper recognition of the interaction that exist between assignment and signal control introduces a number of theoretical and computational complexities; on the other hand considerable network improvements can be achieved. This report introduces the problem of interaction between signal control and traffic assignment, reviews related issues and previous work. It discusses signal control as a network design problem, and it makes a case for the iterative assignment control procedure for its approximate solution

The Interaction Between Route Guidance and Signal Control: Model Results.

A multiple user class assignment model is developed to simulate the interaction between a route guidance system and an urban traffic control system. For two real- life networks of Leeds and Southampton four basic scenarios are investigated: current situation; - expected situation with route guidance but without interaction with the traffic control system; - expected situation with route guidance and with interaction with the traffic control system; - optimum future situation with 100% take-up and compliance with system optimal guidance and delay minimising signal control. The computed extra travel time savings of an integration of route guidance and signal control are of a similar magnitude as those of a route guidance system alone. With static demand delay minimisation performs best, but when demand is dynamically increasing Smith's Po policy gives rise to lowest network travel times. The degrading effect of imperfect advice or non-compliance is small at realistic levels of take-up of up to 25%. Along the same line, the benefits of system optimal guidance are also modest (in the order of less than 1%) when take-up of guidance is less than 25%

The Influence of Cost Assumptions on Properties of the Combined Assignment Control Problems.

The uniqueness and existence of a solution to the combined traffic assignment/signal control problem is investigated, particularly with respect to the cost functions used. The two cost functions investigated are the polynomial BPR function and Webster's two term cost function. Properties of three well-known signal control policies are investigated, and a number of new policies are developed, which guarantee a unique solution to the combined problem. The comparative performance of these policies is tested with respect to uniqueness of the resulting green times and total network travel times at the solution. To this end a streamlined version of the iterative assignment control procedure is developed and applied to three networks. It is found that potential theoretical uniqueness and existence problems do not necessarily occur in practical tests, and that enforcement of theoretical properties on signal control policies renders them rather inefficient

The Evaluation of Route Guidance Systems

BACKGROUND We were commissioned by the Transport and Road Research Laboratory to: "collaborate with the German government and their representatives who are responsible for conducting the LISB trial in Berlin in order to produce an agreed methodology, which is acceptable in both Germany and the UK, for assessing the automatic route guidance systems which will be provided in Berlin and London." The brief suggested a number of aspects to be included, and required detailed proposals, timescales and costs for implementation in London. 1.1.2 The background to the brief lies in decisions to introduce pilot automatic route guidance systems in the two cities. The principles of the systems are similar, and have been described in detail elsewhere (Jeffery, 1987). In brief, they involve : (i) a central computer which retains information on a specified road network, which is updated using real time information from the equipment users; (ii) infra red beacons at selected junctions which transmit information to equipped vehicles and receive information from those vehicles; (iii) in-vehicle equipment which includes a dead-reckoning system for position finding, a device for requesting guidance and specifying the destination, a micro-computer which selects the optimal route, and a display which indicates when a turn is required on the main network, and the compass direction and distance to the final destination; iv) transmission from the equipped vehicles of origin, requested destination, links used since passing the last beacon and, for each link, the time of entry and departure and time spent delayed. It is this travel time information which is used to update the central computer's knowledge of the best routes. (Continues..

Anti-Nucleocapsid Protein Immune Responses Counteract Pathogenic Effects of Rift Valley Fever Virus Infection in Mice

The known virulence factor of Rift Valley fever virus (RVFV), the NSs protein, counteracts the antiviral effects of the type I interferon response. In this study we evaluated the expression of several genes in the liver and spleen involved in innate and adaptive immunity of mice immunized with a RVFV recombinant nucleocapsid protein (recNP) combined with Alhydrogel adjuvant and control animals after challenge with wild type RVFV. Mice immunized with recNP elicited an earlier IFNβ response after challenge compared to non-immunized controls. In the acute phase of liver infection in non-immunized mice there was a massive upregulation of type I and II interferon, accompanied by high viral titers, and the up- and downregulation of several genes involved in the activation of B- and T-cells, indicating that both humoral and cellular immunity is modulated during RVFV infection. Various genes involved in pro-inflammatory responses and with pro-apoptotic effects were strongly upregulated and anti-apoptotic genes were downregulated in liver of non-immunized mice. Expression of many genes involved in B- and T-cell immunity were downregulated in spleen of non-immunized mice but normal in immunized mice. A strong bias towards apoptosis and inflammation in non-immunized mice at an acute stage of liver infection associated with suppression of several genes involved in activation of humoral and cellular immunity in spleen, suggests that RVFV evades the host immune response in more ways than only by inhibition of type I interferon, and that immunopathology of the liver plays a crucial role in RVF disease progression

Biosafety standards for working with Crimean-Congo haemorrhagic fever virus

In countries from which Crimean-Congo haemorrhagic fever (CCHF) is absent, the causative virus CCHF virus (CCHFV) is classified as a hazard group 4 agent and handled in containment level 4. In contrast, most endemic countries out of necessity have had to perform diagnostic tests under biosafety level (BSL) -2 or -3 conditions. In particular, Turkey and several of the Balkan countries have safely processed more than 100000 samples over many years in BSL-2 laboratories. It is therefore advocated that biosafety requirements for CCHF diagnostic procedures should be revised, to allow the required tests to be performed under enhanced BSL-2 conditions with appropriate biosafety laboratory equipment and personal protective equipment used according to standardized protocols in the affected countries. Downgrading of CCHFV research work from Cl-4, BSL-4 to Cl-3, BSL-3 should also be considered.Additional co-authors: Gülay Korukluoglu, Pieter Lyssen, Ali Mirazimi, Johan Neyts, Matthias Niedrig, Aykut Ozkul, Anna Papa, Janusz Paweska, Amadou A Sall, Connie S Schmaljohn, Robert Swanepoel, Yavuz Uyar, Friedemann Weber, Herve Zelle

Can sacrificial feeding areas protect aquatic plants from herbivore grazing? Using behavioural ecology to inform wildlife management

Effective wildlife management is needed for conservation, economic and human well-being objectives. However, traditional population control methods are frequently ineffective, unpopular with stakeholders, may affect non-target species, and can be both expensive and impractical to implement. New methods which address these issues and offer effective wildlife management are required. We used an individual-based model to predict the efficacy of a sacrificial feeding area in preventing grazing damage by mute swans (Cygnus olor) to adjacent river vegetation of high conservation and economic value. The accuracy of model predictions was assessed by a comparison with observed field data, whilst prediction robustness was evaluated using a sensitivity analysis. We used repeated simulations to evaluate how the efficacy of the sacrificial feeding area was regulated by (i) food quantity, (ii) food quality, and (iii) the functional response of the forager. Our model gave accurate predictions of aquatic plant biomass, carrying capacity, swan mortality, swan foraging effort, and river use. Our model predicted that increased sacrificial feeding area food quantity and quality would prevent the depletion of aquatic plant biomass by swans. When the functional response for vegetation in the sacrificial feeding area was increased, the food quantity and quality in the sacrificial feeding area required to protect adjacent aquatic plants were reduced. Our study demonstrates how the insights of behavioural ecology can be used to inform wildlife management. The principles that underpin our model predictions are likely to be valid across a range of different resource-consumer interactions, emphasising the generality of our approach to the evaluation of strategies for resolving wildlife management problems

Vaccination with DNA plasmids expressing Gn coupled to C3d or alphavirus replicons expressing Gn protects mice against rift valley fever virus

Background: Rift Valley fever (RVF) is an arthropod-borne viral zoonosis. Rift Valley fever virus (RVFV) is an important biological threat with the potential to spread to new susceptible areas. In addition, it is a potential biowarfare agent. Methodology/Principal Findings: We developed two potential vaccines, DNA plasmids and alphavirus replicons, expressing the Gn glycoprotein of RVFV alone or fused to three copies of complement protein, C3d. Each vaccine was administered to mice in an all DNA, all replicon, or a DNA prime/replicon boost strategy and both the humoral and cellular responses were assessed. DNA plasmids expressing Gn-C3d and alphavirus replicons expressing Gn elicited high titer neutralizing antibodies that were similar to titers elicited by the live-attenuated MP12 virus. Mice vaccinated with an inactivated form of MP12 did elicit high titer antibodies, but these antibodies were unable to neutralize RVFV infection. However, only vaccine strategies incorporating alphavirus replicons elicited cellular responses to Gn. Both vaccines strategies completely prevented weight loss and morbidity and protected against lethal RVFV challenge. Passive transfer of antisera from vaccinated mice into naïve mice showed that both DNA plasmids expressing Gn-C3d and alphavirus replicons expressing Gn elicited antibodies that protected mice as well as sera from mice immunized with MP12. Conclusion/Significance: These results show that both DNA plasmids expressing Gn-C3d and alphavirus replicons expressing Gn administered alone or in a DNA prime/replicon boost strategy are effective RVFV vaccines. These vaccine strategies provide safer alternatives to using live-attenuated RVFV vaccines for human use. © 2010 Bhardwaj et al