Characterization of carboxymethyl cellulase produced by Trichoderma asperellum

Background: The present study characterized extracellular carboxymethyl cellulase (CMCase), an enzyme catalyzes the hydrolysis of the cellulose, from Trichoderma asperellum PQ34 that was isolated from agricultural cultivation soil in Thua Thien Hue, Vietnam.Methods: CMCase was produced by culturing T. asperellum PQ34 on Czapek-Dox medium supplemented with carboxymethyl cellulose (CMC) at 28ºC for 96 h at a shaking speed of 150 rpm. Enzyme activity was spectrophotometerically determined with CMC used as a substrate and the absorbance was measured at 540 nm. The molecular weight of CMCase was determined by zymogram based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with 0.2% CMC.Results: CMCase achieved the highest activity after 4 days of culture with the optimal pH and temperature at 4 and 55°C. The enzyme maintains pH and thermal stability in the range of 4-5 and 10-40°C and the relative activity of both is more than 90%. The presence of metal ions at 5 mM such as Ca2+, Al3+, or Co2+ increased the enzyme activity up to 133%, 124%, and 217%, respectively. CMCase was stable with 1 mM ethylenediaminetetraacetic acid and 1 M urea, but inhibited by 5% sodium dodecyl sulfate (SDS) and 5% Triton-X100. Zymographic analysis showed that CMCase from T. asperellum PQ34 consists of two enzymes with a molecular weight of about 31 and 66 kDa.Conclusion: CMCases from T. asperellum PQ34 exhibited characteristics of an acidic and thermostable enzyme. The interaction of Co2+ and CMCase resulted in a significant increase in enzyme activity, however they were strongly inhibited by 5% SDS.Keywords: Carboxymethyl cellulase; Cellulase; Endo-β-1,4-glucanase, Trichoderma asperellu

Influence of damping coefficient into engine rubber mounting system on vehicle ride comfort

This study presents a method to improve vehicle ride comfort using additional damping coefficient values for an internal combustion engine (ICE) rubber mounting system. To analyze the effect of the adding damping coefficient values into the rubber mounting system on vehicle ride comfort, a full-vehicle vibration model with 10 degrees of freedom is established under the combination of road surface roughness and ICE excitations. The damping coefficient values are added into ICE rubber mounting system which are respectively analyzed and evaluated according to the international standard ISO 2631-1 (1997). The study results do not only evaluate the influence of the adding damping coefficients on vehicle ride comfort but also suggest the optimal design solution for ICE mounting system to improve vehicle ride comfort

Outage probability analysis of EH relay-assisted non-orthogonal multiple access (NOMA) systems over Block Rayleigh Fading Channel

Non-orthogonal multiple access (NOMA) has been identified as a promising multiple access technique for the fifth generation (5G) mobile networks due to its superior spectral efficiency. In this paper, we propose and investigate a Non-Orthogonal Multiple Access (NOMA) of energy harvesting (EH) relay assisted system over Block Rayleigh Fading Channel. In order to evaluate the performance of the proposed system, the integral expression of the outage probability is analyzed and derived. Numerical results confirm that our derived analytical results match well with the Monte Carlo simulations in connection with all possible system parameter

Performance analysis for power-splitting energy harvesting based two-way full-duplex relaying network over nakagami-m fading channel

Energy harvesting relay network is considered as the promising solution for a wireless communication network in our time. In this research, we present and demonstrate the system performance of the energy harvesting based two-way full-duplex relaying network over Nakagami-m fading environment. Firstly, we propose the analytical expressions of the achievable throughput and outage probability of the proposed system. In the second step, the effect of various system parameters on the system performance is presented and investigated. In the final step, the analytical results are also demonstrated by Monte-Carlo simulation. The numerical results demonstrated and convinced the analytical and the simulation results are agreed with each other

Half-duplex power beacon-assisted energy harvesting relaying networks: system performance analysis

In this work, the half-duplex (HF) power beacon-assisted (PB) energy harvesting (EH) relaying network, which consists of a source (S), Relay (R), destination (D) and a power beacon (PB) are introduced and investigated. Firstly, the analytical expressions of the system performance in term of outage probability (OP) and the system throughput (ST) are analyzed and derived in both amplify-and-forward (AF) and decode-and-forward (DF) modes. After that, we verify the correctness of the analytical analysis by using Monte-Carlo simulation in connection with the primary system parameters. From the numerical results, we can see that all the analytical and the simulation results are matched well with each other

Time Resolved TDLAS Investigation of Pre-ionization Effect in Dual HiPIMS Discharges

Time-resolved measurements have been performed during dual High Power Impulse Magnetron Sputtering (dual-HiPIMS) with two cathodes in a closed magnetic field configuration. The dual-HiPIMS system, operated at a repetition frequency f = 100 Hz and duty cycle of 1\%, was equipped with two different metallic targets (Ti, Cu). Pre-ionization effect of the Ti pulse on the Cu pulse was investigated through argon excited atom density and temperature by means of tunable diode laser absorption spectroscopy. We observed an enhancement of metastable density due to pre-ionization effect. It is shown that the peak density in the Cu pulse increases about 30\% for the case of 15$\mu$s delay then decrease as the delay was prolonged. The effect displays even stronger in the peak density in the afterglow, as well as in the atom temperature behavior. Although metastable atoms are abundantly available in post-discharge, they play minor role in the ignition of the subsequence pulse

Optimal power generation for wind-hydro-thermal system using meta-heuristic algorithms

In this paper, Cuckoo search algorithm (CSA) is suggested for determining optimal operation parameters of the combined wind turbine and hydrothermal system (CWHTS) in order to minimize total fuel cost of all operating thermal power plants while all constraints of plants and system are exactly satisfied. In addition to CSA, Particle swarm optimization (PSO), PSO with constriction factor and inertia weight factor (FCIWPSO) and Social Ski-Driver (SSD) are also implemented for comparisons. The CWHTS is optimally scheduled over twenty-four one-hour interval and total cost of producing power energy is employed for comparison. Via numerical results and graphical results, it indicates CSA can reach much better results than other ones in terms of lower total cost, higher success rate and faster search process. Consequently, the conclusion is confirmed that CSA is a very efficient method for the problem of determining optimal operation parameters of CWHTS

NOWJ1@ALQAC 2023: Enhancing Legal Task Performance with Classic Statistical Models and Pre-trained Language Models

This paper describes the NOWJ1 Team's approach for the Automated Legal Question Answering Competition (ALQAC) 2023, which focuses on enhancing legal task performance by integrating classical statistical models and Pre-trained Language Models (PLMs). For the document retrieval task, we implement a pre-processing step to overcome input limitations and apply learning-to-rank methods to consolidate features from various models. The question-answering task is split into two sub-tasks: sentence classification and answer extraction. We incorporate state-of-the-art models to develop distinct systems for each sub-task, utilizing both classic statistical models and pre-trained Language Models. Experimental results demonstrate the promising potential of our proposed methodology in the competition.Comment: ISAILD@KSE 202

Outage and bit error probability analysis in energy harvesting wireless cooperative networks

This study focuses on a wireless powered cooperative communication network (WPCCN), which includes a hybrid access point (HAP), a source and a relay. The considered source and relay are installed without embedded energy supply (EES), thus are dependent on energy harvested from signals from the HAP to power their cooperative information transmission (IT). Taking inspiration from this, the author group investigates into a harvest-then-cooperate (HTC) protocol, whereas the source and the relay first harvest the energy from the AP in a downlink (DL) and then collaboratively work in uplink (UL) for IT of the source. For careful evaluation of the system performance, derivations of the approximate closed-form expression of the outage probability (OP) and an average bit error probability ( ABER) for the HTC protocol over Rayleigh fading channels are done. Lastly, the author group performs Monte-Carlo simulations to reassure the numerical results they obtained.Web of Science255746