Depressed RNA synthesis in the brains and livers of thyroidectomized, normal and hormone injected rats

Depression of rat brain and liver RNA synthesis was seen at 22 and 90 days subsequent to neonatal radiothyroidectomy. Cerebral RNA synthesis (per unit DNA) was depressed by 16% (P P in vitro assay systems which circumvented complications (often seen in vivo approaches) such as pool size changes, cell membrane transport alterations and mediation of blood-brain barrier related effects.Chronic daily administration in vivo of -thyroxine (10 [mu]g/100 g body weight) restored the depressed cerebral synthesis of RNA to normal levels while daily administration of bovine growth hormone (100 [mu]g per rat) did not.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34101/1/0000383.pd

Physical, Chemical, and Geological Changes of Marine Environment Caused by the Benthic Impact Experiment at the I0M BIE Site

In order to predict potential effects of mining of polymetallic nodules, Interoceanmetal Joint Organization (I0M) carried out, in 1995, a Benthic Impact Experiment (I0M BIE) in its pioneer area within the Clarion-Clipperton Fracture Zone. The I0M BIE study involved pre-disturbance (baseline), disturbance and postdisturbance stages as well as subsequent monitoring sampling in 1997. Comparison of data on physical and chemical characteristics of bottom sediment, near·bottom and pore water collected before and after experiment showed alterations in those characteristics. Contents of nutrients (silicates, nitrates, nitrites, and phosphates) and heavy metals (Cu, Zn, Pb, Cd) as well as the mineralogical and grain size composition of the discharged sediments were determined on material collected by sediment traps, water bottles, and a multiple corer. Preliminary data on characteristics of the disturbed and resettled sediment layer obtained during the 1997 monitoring cruise are presented and discussed

Complete mitochondrial genome of the abyssal coral Abyssoprimnoa gemina Cairns, 2015 (Octocorallia, Primnoidae) from the Clarion-Clipperton Zone, Pacific Ocean

The Clarion-Clipperton Zone (CCZ) in the tropical East Pacific is a region of interest for deep-sea mining due to its underwater deposits of polymetallic nodules containing economically important metals such as nickel, copper, and cobalt. It is also a region of extensive baseline studies aiming to describe the state of the environment, including the biodiversity of the benthic fauna. An abundant component of the abyssal plain ecosystem consists of sessile fauna which encrusts polymetallic nodules and are vulnerable to potential impacts arising from exploitation activities, particularly removal of substrate. Therefore, this fauna is often considered to have key species whose genetic connectivity should be studied to assess their ecological resilience. One such species is Abyssoprimnoa gemina Cairns, 2015, a deep-sea coral from the CCZ whose presence in the Interoceanmetal Joint Organization (IOM) claim area has been confirmed during samplings. In this study, we used next-generation sequencing (NGS) to obtain the 18S nuclear rRNA gene and the complete mitochondrial genome of A. gemina from IOM exploration area. The mitogenome is 18,825 bp long and encodes for 14 protein coding genes, 2 rRNAs, and a single tRNA. The two phylogeny reconstructions derived from these data confirm previous studies and display A. gemina within a highly supported cluster of seven species whose mitogenomes are all colinear and of comparable size. This study also demonstrates the suitability of NGS for DNA barcoding of the benthic megafauna of the CCZ, which could become part of the IOM protocol for the assessment of population diversity and genetic connectivity in its claim area