Intra and inter-rater reliability study of pelvic floor muscle dynamometric measurements

The aim of this study was to evaluate the intra and inter-rater reliability of pelvic floor muscle (PFM) dynamometric measurements for maximum and average strengths, as well as endurance. A convenience sample of 18 nulliparous women, without any urogynecological complaints, aged between 19 and 31 (mean age of 25.4±3.9) participated in this study. They were evaluated using a pelvic floor dynamometer based on load cell technology. The dynamometric evaluations were repeated in three successive sessions: two on the same day with a rest period of 30 minutes between them, and the third on the following day. All participants were evaluated twice in each session; first by examiner 1 followed by examiner 2. The vaginal dynamometry data were analyzed using three parameters: maximum strength, average strength, and endurance. The Intraclass Correlation Coefficient (ICC) was applied to estimate the PFM dynamometric measurement reliability, considering a good level as being above 0.75. The intra and inter-raters' analyses showed good reliability for maximum strength (ICCintra-rater1=0.96, ICCintra-rater2=0.95, and ICCinter-rater=0.96), average strength (ICCintra-rater1=0.96, ICCintra-rater2=0.94, and ICCinter-rater=0.97), and endurance (ICCintra-rater1=0.88, ICCintra-rater2=0.86, and ICCinter-rater=0.92) dynamometric measurements. The PFM dynamometric measurements showed good intra- and inter-rater reliability for maximum strength, average strength and endurance, which demonstrates that this is a reliable device that can be used in clinical practice.1929710

Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties.

Casein, a protein found in milk of several species, is divided into different chains from 19 to 25 kDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others. In this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation purified NZCase TT, an industrial culture media for tetanus toxin production. In the first step, four pools were isolated and tested in different assays: isolated smooth muscle assay (guinea pig ileum, rat uterus), phagocytosis in vitro of opsonized sheep red blood cells, and hydrogen peroxide (H2O2) release from mouse peritoneal macrophages. Pool III was the main active pool being able to potentiate bradykinin action in guinea pig ileum, stimulating phagocitic activity by resident macrophages and increasing H2O2 release from macrophages previously activated with bacille Calmette Guérin. Using mass spectra the primary structure of the main peptide from pool III was obtained--INKKI, which corresponds to beta-casein fragment 26-30. The immunostimulating action is probably related to a direct action in macrophage cells

Fur controls iron homeostasis and oxidative stress defense in the oligotrophic alpha-proteobacterium Caulobacter crescentus

In most bacteria, the ferric uptake regulator (Fur) is a global regulator that controls iron homeostasis and other cellular processes, such as oxidative stress defense. In this work, we apply a combination of bioinformatics, in vitro and in vivo assays to identify the Caulobacter crescentus Fur regulon. A C. crescentus fur deletion mutant showed a slow growth phenotype, and was hypersensitive to H2O2 and organic peroxide. Using a position weight matrix approach, several predicted Fur-binding sites were detected in the genome of C. crescentus, located in regulatory regions of genes not only involved in iron uptake and usage but also in other functions. Selected Fur-binding sites were validated using electrophoretic mobility shift assay and DNAse I footprinting analysis. Gene expression assays revealed that genes involved in iron uptake were repressed by iron-Fur and induced under conditions of iron limitation, whereas genes encoding iron-using proteins were activated by Fur under conditions of iron sufficiency. Furthermore, several genes that are regulated via small RNAs in other bacteria were found to be directly regulated by Fur in C. crescentus. In conclusion, Fur functions as an activator and as a repressor, integrating iron metabolism and oxidative stress response in C. crescentus

Fur controls iron homeostasis and oxidative stress defense in the oligotrophic alpha-proteobacterium Caulobacter crescentus

In most bacteria, the ferric uptake regulator (Fur) is a global regulator that controls iron homeostasis and other cellular processes, such as oxidative stress defense. In this work, we apply a combination of bioinformatics, in vitro and in vivo assays to identify the Caulobacter crescentus Fur regulon. A C. crescentus fur deletion mutant showed a slow growth phenotype, and was hypersensitive to H2O2 and organic peroxide. Using a position weight matrix approach, several predicted Fur-binding sites were detected in the genome of C. crescentus, located in regulatory regions of genes not only involved in iron uptake and usage but also in other functions. Selected Fur-binding sites were validated using electrophoretic mobility shift assay and DNAse I footprinting analysis. Gene expression assays revealed that genes involved in iron uptake were repressed by iron-Fur and induced under conditions of iron limitation, whereas genes encoding iron-using proteins were activated by Fur under conditions of iron sufficiency. Furthermore, several genes that are regulated via small RNAs in other bacteria were found to be directly regulated by Fur in C. crescentus. In conclusion, Fur functions as an activator and as a repressor, integrating iron metabolism and oxidative stress response in C. crescentus

Leaf Appearance and Elongation in Panicum maximum cv. Tanzania Tillers of Varying Ages

A sward may be considered as a tiller population of varying ages and sizes, and these different age groups are likely to present distinct behaviour in terms of growth and herbage production. However, there is very little information on how tiller age, in association with grazing management practices (e.g. frequency and intensity of grazing), alter morphogenetic characteristics and, therefore, herbage production. Against this background, the present experiment had the objective to evaluate leaf appearance and elongation in Panicum maximum cv. Tanzania tillers of different age groups when submitted to intermittent grazing regimes

Herbage Production of Tanzania Grass (\u3cem\u3ePanicum maximum\u3c/em\u3e cv. Tanzania) Submitted to Combinations of Frequencies and Intensities of Grazing by Cattle

Animal production from pastures is a complex process comprising three main stages: herbage growth, consumption by grazing animals and conversion into animal products (Hodgson, 1990). Utilisation is the stage where the grazier finds greater flexibility for management, probably because most processes related to harvest of the produced herbage by the grazing animals are very responsive to manipulation and control of defoliation practices. The objective of this study was to evaluate herbage production of a Panicum maximum cv. Tanzania pasture submitted to combinations of frequencies and intensities of grazing

Probiotics in Aquaculture Review: Current Status and Application in Tambaqui Cultivation (Colossoma macropomum)

The development of aquaculture guarantees the supply of animal protein of great nutritional value, contributing to food security. Currently one of the main problems faced is the occurrence of diseases, responsible for a worldwide economic loss, equivalent to US $ 9 billion per year. Aiming to increase resistance to diseases, increasing growth rates and food efficiency in intensive crops, some strategies have been developed, one of them is the use of probiotic bacteria. These, when in contact with the digestive tract of the host generates a series of benefits, among them, the modulation of the immune system, developing defense mechanisms and increasing resistance to stress. However, there are few documented reports on the efficiency of probiotics in native species, such as tambaqui (Colossoma macropomum). This species shows some resistance to stress, through physiological mechanisms of adaptation, such as lip expansion when subjected to hypoxia situations, which added to the positive effects of using probiotics would represent an increase in its resistance. The purpose of this work is to review the literature on the use of probiotics in aquaculture in order to provide a comprehensive synthesis of the current knowledge about its use in aquaculture, with emphasis on the intensive cultivation of tambaqui

L-Asparaginase

L-Asparaginase (ASNase, EC 3.5.1.1) is a tetrameric aminohydrolase enzyme that catalyses the hydrolysis of the amino acid L-Asparagine into ammonia and L-aspartic acid. ASNase is present in different organisms such as bacteria, fungi, plant tissues and algae. ASNase is used in the pharmaceutical field as an anticancer drug for the treatment of acute lymphoblastic leukemia (ALL) and other malignant diseases such as Hodgkin’s disease. In the food sector, ASNase is used to prevent the formation of acrylamide, a toxic compound formed when starch-rich foods are cooked at temperatures above 100 °C. ASNase can also be used as a biosensor for the detection of L-asparagine levels.publishe

Herbage Accumulation and Animal Performance on Xaraes Palisade Grass Subjected to Intensities of Continuous Stocking Management

The large majority of species used in Brazil belong to the Brachiaria and Panicum genus, with marandu paladisadegrass (Brachiaria brizantha (Hochst. ex A. Rich) cv. Marandu) being the main cultivated forage grass species (Santos Filho 1996). Brachiaria brizantha cv. xaraés (xaraes palisade grass) was released as an option for diversifying forage species, and it had the advantages of fast regrowth and herbage yield, favouring larger stocking rates and animal productivity (Euclides et al. 2008; 2009). The objective of this study was to evaluate herbage accumulation and animal performance of beef cattle steers grazing continuously stocked xaraes palisade grass managed at 15, 30 and 45 cm