278 research outputs found
Quantification of gliadin levels to the picogram level by flow cytometry
Celiac disease is a widely prevalent enteropathy caused by intolerance to gliadin, one of the gluten proteins. We developed two methods for the analysis of gliadin levels. Both methods use flow cytometry and rat antibodies against a 16-residue peptide of gliadin. The peptide is common to the alpha-, beta-, gamma-, and omega-gliadins
Stellar population and the origin of intra-cluster stars around brightest cluster galaxies: the case of NGC 3311
Context. We investigate the stellar population and the origin of diffuse
light around brightest cluster galaxies.
Aims. We study the stellar population of the dynamically hot stellar halo of
NGC 3311, the brightest galaxy in the Hydra I cluster, and that of photometric
substructures in the diffuse light to constrain the origin of these components.
Methods. We analyze absorption lines in medium-resolution, long-slit spectra
in the wavelength range 4800-5800 angstrom obtained with FORS2 at the Very
Large Telescope. We measure the equivalent width of Lick indices out to 20 kpc
from the center of NGC 3311 and fit them with stellar population models that
account for the [alpha/Fe] overabundance.
Results. Stars in the dynamically hot halo of NGC 3311 are old (age > 13
Gyr), metal-poor ([Z/H] ~ -0.35), and alpha-enhanced ([alpha/Fe] ~ 0.48).
Together with the high velocity dispersion, these measurements indicate that
the stars in the halo were accreted from the outskirts of other early-type
galaxies, with a possible contribution from dwarf galaxies. We identify a
region in the halo of NGC 3311 associated with a photometric substructure where
the stellar population is even more metal-poor ([Z/H] ~ -0.73). In this region,
our measurements are consistent with a composite stellar population superposed
along the line of sight, consisting of stars from the dynamically hot halo of
NGC 3311 and stars stripped from dwarf galaxies. The latter component
contributes < 28% to the local surface brightness.
Conclusions. The build-up of diffuse light around NGC 3311 is on-going. Based
on the observed stellar population properties, the dominant part of these stars
may have come from the outskirts of bright early-type galaxies, while stars
from stripped dwarf galaxies are presently being added.Comment: 8 pages, 4 figures. Accepted for publication in Astronomy &
Astrophysic
An integrated technological approach to the selection of lactic acid bacteria of flour origin for sourdough production
Several lactic acid bacteria (LAB) were evaluated in situ for their potential in sourdough fermentation. The
strains belonged to Lactobacillus plantarum, Lactobacillus sakei, Lactobacillus sanfranciscensis, Leuconostoc citreum,
Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides and Weissella cibaria. LAB were used, in individual
inocula, to carry out the fermentation of \u3b3-ray treated (sterile) flour and untreated commercial flour, in order
to evaluate their performances both in the absence and presence of the native microbiota of flour. The pH and
total titratable acidity (TTA) showed a strong and fast acidification of the experimental sourdough determined
by W. cibaria and Ln. citreum strains. All strains were followed during fermentation by plate count. Randomly
amplified polymorphic DNA (RAPD)-PCR analysis applied on the colonies isolated from the highest dilution of
samples confirmed the dominance of the added strains in all sourdoughs prepared with sterile and non-sterile
flour. The analysis of organic acids, performed by high-performance liquid chromatography (HPLC), confirmed
that some W. cibaria and Ln. citreum strains showed an optimal fermentation quotient. The volatile organic
compound (VOC) composition resulting from the gas chromatography coupled with mass spectrometry (GC/MS)
analysis of sourdough headspace recognised 51 chemical compounds including acids, alcohols, aldehydes, esters,
ketones, lactones, acetate, alkane, and phenol, most of which are of LAB origin and are relevant for the final bread.
After baking, the breads were evaluated for the height of the central slices, colour of crust and crumb, hardness
and number and distribution of alveolus. The combination of these results indicated that strains Ln. citreum
PON10079 and PON10080 and W. cibaria PON10030 and PON10032 are suitable cultures to use in industrial
production
The dynamically hot stellar halo around NGC 3311: a small cluster-dominated central galaxy
An important open question is the relation between intracluster light and the
halos of central galaxies in galaxy clusters. Here we report results from an on
going project with the aim to characterize the dynamical state in the core of
the Hydra I (Abell 1060) cluster around NGC 3311. Methods: We analyze deep
long-slit absorption line spectra reaching out to ~25 kpc in the halo of NGC
3311. Results: We find a very steep increase in the velocity dispersion profile
from a central sigma_0=150 km/s to sigma_out ~450 km/s at R ~ 12 kpc. Farther
out, to ~25 kpc, sigma appears to be constant at this value, which is ~60% of
the velocity dispersion of the Hydra I galaxies. With its dynamically hot halo
kinematics, NGC 3311 is unlike other normal early-type galaxies. Conclusions:
These results and the large amount of dark matter inferred from X-rays around
NGC 3311 suggest that the stellar halo of this galaxy is dominated by the
central intracluster stars of the cluster, and that the transition from
predominantly galaxy-bound stars to cluster stars occurs in the radial range 4
to 12 kpc from the center of NGC 3311. We comment on the wide range of halo
kinematics observed in cluster central galaxies, depending on the evolutionary
state of their host clusters.Comment: 5 pages, 4 figures, 1 table, accepted for publication in A&
SHBG levels in primary infertile men : A critical interpretation in clinical practice
Objective: We aimed to test the association between age, BMI and sex-hormone\u2013binding globulin (SHBG) in a homogenous cohort of white-European men presenting for primary couple\u2019s infertility. Design: Retrospective study. Methods: Data from 1547 infertile men were analysed. Health-significant comorbidities were scored with the Charlson comorbidity index (CCI). Fasting serum hormones were measured in every patient. Age was considered according to quartile groups (<33, 33-41, >41 years) and BMI as normal weight (18.5\u201324.9 kg/m2), overweight (25.0\u201329.9 kg/m2) and obesity (>30 kg/m2). Descriptive statistics and linear regression analysis tested the associations between age, BMI and SHBG. Results: Median SHBG levels increased across quartiles of age and decreased along with BMI increases (all P < 0.001). For each year increase in age, SHBG increased 0.32 nmol/L; conversely, for each unit increase in BMI, SHBG decreased by 1.1 nmol/L (all P < 0.001). SHBG levels decline with increasing BMI was greater than SHBG progressive increase with age. Overall, BMI explained 3.0 times more of the variability in SHBG than did ageing. At multivariate linear model, age and BMI were the most significant factors influencing SHBG concentration (all P < 0.001), after accounting for CCI, albumin levels and smoking status. Conclusions: We found a wide distribution of SHBG concentrations across age and BMI values in primary infertile men. The association between BMI and lowered SHBG levels seems to be greater than the association of ageing with increased SHBG
Influence of stabilisers on the catalytic activity of supported Au colloidal nanoparticles for the liquid phase oxidation of glucose to glucaric acid: understanding the catalyst performance from NMR relaxation and computational studies
Supported Au colloidal nanoparticles have been prepared in the presence of stabilising polymers, such as, PVA, PVP and PEG (polyvinylalcohol, polyvinylpyrrolidone, polyethylene glycol). The effect of the polymer to Au weight ratio was investigated, for the synthesis of Au nanoparticles with varying particle size and particle size distribution. By varying the polymer/Au wt/wt ratio, Au nanoparticles with mean diameters from 3 to 8 nm were synthesised. The synthesised Au catalysts were studied in the liquid phase oxidation of glucose to glucaric acid under alkaline conditions. We demonstrated that the choice of polymer and polymer to Au weight ratio, have an important influence in terms of catalytic activity and yield to glucaric acid. The highest yield to glucaric acid (22%) was obtained using Au–PVA catalysts. A strong deactivation was observed using Au catalysts. Further evaluation of the possible reasons for deactivation were investigated using experimental, computational and NMR relaxation studies
Redox regulation of cellular stress response in multiple sclerosis
Multiple sclerosis (MS) is an autoimmune-mediated neurodegenerative disease with characteristic foci of inflammatory demyelination in the brain, spinal cord, and optic nerves. Recent studies have demonstrated not only that axonal damage and neuronal loss are significant pathologic components of MS, but that this neuronal damage is thought to cause the permanent neurologic disability often seen in MS patients. Emerging finding suggests that altered redox homeostasis and increased oxidative stress, primarily implicated in the pathogenesis of MS, are a trigger for activation of a brain stress response. Relevant to maintenance of redox homeostasis, integrated mechanisms controlled by vitagenes operate in brain in preserving neuronal survival during stressful conditions. Vitagenes encode for heat shock proteins (Hsp) Hsp32, Hsp70, the thioredoxin and the sirtuin protein systems. In the present study we assess stress response mechanisms in the CSF, plasma and lymphocytes of control patients compared to MS patients. We found that the levels of vitagenes Hsp72, Hsc70, HO-1, as well as oxidative stress markers carbonyls and hydroxynonenals were significantly higher in the blood and CSF of MS patients than in control patients. In addition, an increased expression of Trx and sirtuin 1, together with a decrease in the expression of TrxR were observed. Our data strongly support a pivotal role for redox homeostasis disruption in the pathogenesis of MS and, consistently with the notion that new therapies that prevent neurodegeneration through nonimmunomodulatory mechanisms can have a tremendous potential to work synergistically with current MS therapies, unravel important targets for new cytoprotective strategies
The Properties of X-ray Cold Fronts in a Statistical Sample of Simulated Galaxy Clusters
We examine the incidence of cold fronts in a large sample of galaxy clusters
extracted from a (512h^-1 Mpc) hydrodynamic/N-body cosmological simulation with
adiabatic gas physics computed with the Enzo adaptive mesh refinement code.
This simulation contains a sample of roughly 4000 galaxy clusters with M >
10^14 M_sun at z=0. For each simulated galaxy cluster, we have created mock
0.3-8.0 keV X-ray observations and spectroscopic-like temperature maps. We have
searched these maps with a new automated algorithm to identify the presence of
cold fronts in projection. Using a threshold of a minimum of 10 cold front
pixels in our images, corresponding to a total comoving length L_cf > 156h^-1
kpc, we find that roughly 10-12% of all projections in a mass-limited sample
would be classified as cold front clusters. Interestingly, the fraction of
clusters with extended cold front features in our synthetic maps of a
mass-limited sample trends only weakly with redshift out to z=1.0. However,
when using different selection functions, including a simulated flux limit, the
trending with redshift changes significantly. The likelihood of finding cold
fronts in the simulated clusters in our sample is a strong function of cluster
mass. In clusters with M>7.5x10^14 M_sun the cold front fraction is 40-50%. We
also show that the presence of cold fronts is strongly correlated with
disturbed morphology as measured by quantitative structure measures. Finally,
we find that the incidence of cold fronts in the simulated cluster images is
strongly dependent on baryonic physics.Comment: 16 pages, 21 figures, Accepted to Ap
Diffuse light and galaxy interactions in the core of nearby clusters
The kinematics of the diffuse light in the densest regions of the nearby
clusters can be unmasked using the planetary nebulae (PNs) as probes of the
stellar motions. The position-velocity diagrams around the brightest cluster
galaxies (BCGs) identify the relative contributions from the outer halos and
the intracluster light (ICL), defined as the light radiated by the stars
floating in the cluster potential. The kinematics of the ICL can then be used
to asses the dynamical status of the nearby cluster cores and to infer their
formation histories. The cores of the Virgo and Coma are observed to be far
from equilibrium, with mergers currently on-going, while the ICL properties in
the Fornax and Hydra clusters show the presence of sub-components being
accreted in their cores, but superposed to an otherwise relaxed population of
stars. Finally the comparison of the observed ICL properties with those
predicted from Lambda-CDM simulations indicates a qualitative agreement and
provides insights on the ICL formation. Both observations and simulations
indicate that BCG halos and ICL are physically distinct components, with the
``hotter" ICL dominating at large radial distances from the BCGs halos as the
latter become progressively fainter.Comment: 14 pages, 5 figures. Invited review to appear in the proceedings of
"Galaxies and their masks" eds. Block, D.L., Freeman, K.C. and Puerari, I.,
2010, Springer (New York
Impact of stalling events on microcirculatory hemodynamics in the aged brain
ABSTRACT: Objective The role of cerebral microvasculature in cognitive dysfunction can be investigated by identifying the impact of blood flow on cortical tissue oxygenation. In this paper, the impact of capillary stalls on microcirculatory characteristics such as flow and hematocrit (Ht) in the cortical angioarchitecture is studied. Methods Using a deterministic mathematical model to simulate blood flow in a realistic mouse cortex, hemodynamics parameters, including pressure, flow, vessel diameter-adjustable hematocrit, and transit time are calculated as a function of stalling events. Results Using a non-linear plasma skimming model, it is observed that Ht increases in the penetrating arteries from the pial vessels as a function of cortical depth. The incidence of stalling on Ht distribution along the blood network vessels shows reduction of RBCs around the tissue near occlusion sites and decreased Ht concentration downstream from the blockage points. Moreover, upstream of the occlusion, there is a noticeable increase of the Ht, leading to larger flow resistance due to higher blood viscosity. We predicted marked changes in transit time behavior due to stalls which match trends observed in mice in vivo. Conclusions These changes to blood cell quantity and quality may be implicated in the development of Alzheimer's disease and contribute to the course of the illness
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