502 research outputs found
A gradient-forming MipZ protein mediating the control of cell division in the magnetotactic bacterium Magnetospirillum gryphiswaldense
Cell division needs to be tightly regulated and closely coordinated with other cellular processes to ensure the generation of fully viable offspring. Here, we investigate division site placement by the cell division regulator MipZ in the alphaproteobacterium Magnetospirillum gryphiswaldense, a species that forms linear chains of magnetosomes to navigate within the geomagnetic field. We show that M. gryphiswaldense contains two MipZ homologs, termed MipZ1 and MipZ2. MipZ2 localizes to the division site, but its absence does not cause any obvious phenotype. MipZ1, by contrast, forms a dynamic bipolar gradient, and its deletion or overproduction cause cell filamentation, suggesting an important role in cell division. The monomeric form of MipZ1 interacts with the chromosome partitioning protein ParB, whereas its ATP-dependent dimeric form shows non-specific DNA-binding activity. Notably, both the dimeric and, to a lesser extent, the monomeric form inhibit FtsZ polymerization in vitro. MipZ1 thus represents a canonical gradient-forming MipZ homolog that critically contributes to the spatiotemporal control of FtsZ ring formation. Collectively, our findings add to the view that the regulatory role of MipZ proteins in cell division is conserved among many alphaproteobacteria. However, their number and biochemical properties may have adapted to the specific needs of the host organism
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Inverse Modeling to Quantify Irrigation System Characteristics and Operational Management
Remotely sensed (RS) data is a major source to obtain spatial data required for hydrological models. The challenge for the future is to obtain besides the more direct observable data (landcover, leaf area index, digital elevation model and evapotranspiration), non-visible data such as soil characteristics, groundwater depth and irrigation practices.In this study we have explore the option of using inverse modeling to obtain these non-RS-visible data. For a command area in Haryana, India, we applied for the 2000–2001 rabi season a RS-GIS-combined inverse modeling approach to derive non-RS-visible data required in the regional application of hydrological models. A Genetic Algorithm loaded stochastic physically based soil-water-atmosphere-plant model (SWAP) was developed for the inverse problem and used in the study. The results showed good agreement with the inventoried data such as soil hydraulic properties, sowing dates, ground water depths, irrigation practices and water quality. The derived data could be used to predict the state of the system at anytime in the cropping season, which can be used to evaluate operational management strategies
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On quantifying agricultural and water management practices from low spatial resolution RS data using genetic algorithms: A numerical study for mixed-pixel environment
In this paper, we present a genetic algorithm-based methodology to quantify agricultural and water management practices from remote sensing (RS) data in a mixed-pixel environment. First, we formulated a linear mixture model for low spatial resolution RS data where we considered three agricultural land uses as dominant inside the pixel—rainfed, irrigated with two, and three croppings a year; the mixing parameters we considered were the sowing dates, area fractions of agricultural land uses in the pixel, and their corresponding water management practices. Then, we carried out numerical experiments to evaluate the feasibility of the proposed approach. In the process, the mixing parameters were parameterized by data assimilation using evapotranspiration and leaf area index as conditioning criteria. The soil–water–atmosphere–plant system model SWAP was used to simulate the dynamics of these two biophysical variables in the pixel. The results of our numerical experiments showed that it is possible to derive some sub-pixel information from low spatial resolution data e.g. the existing agricultural and water management practices in a region, which are relevant for regional agricultural monitoring programs
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Bias correction of daily GCM rainfall for crop simulation studies
General circulation models (GCMs), used to predict rainfall at a seasonal lead-time, tend to simulate too many rainfall events of too low intensity relative to individual stations within a GCM grid cell. Even if bias in total rainfall is corrected relative to a target location, this distortion of frequency and intensity is expected to adversely affect simulations of crop growth and yield. We present a procedure that calibrates both the frequency and the intensity distribution of daily GCM rainfall relative to a target station, and demonstrate its application to maize yield simulation at a location in semi-arid Kenya. If GCM rainfall frequency is greater than observed frequency for a given month, averaged across years, GCM rainfall frequency is corrected by discarding rainfall events below a calibrated threshold. To correct the intensity distribution, each GCM rainfall amount above the calibrated threshold is mapped from the GCM intensity distribution onto the observed distribution. We used a gamma distribution for observed rainfall intensity, and considered both gamma and empirical distributions for GCM rainfall intensity. At the study location, the proposed correction procedure corrected both the mean and variance of monthly and seasonal GCM rainfall total, frequency and mean intensity. The empirical (GCM)-gamma (observed) transformation overestimated mean intensity slightly. A simple multiplicative shift did a better job of correcting monthly and seasonal rainfall totals, but left substantial frequency and intensity bias. All of the bias correction procedures improved maize yield simulations, but resulted in substantial negative mean bias. This bias appears to be associated with a tendency for the GCM rainfall to be more strongly autocorrelated than observed rainfall, resulting in unrealistically long dry spells during the growing season. Nonlinearity of crop response to the variability of water availability across GCM realizations may also contribute. Averaging simulated yields each year across multiple GCM realizations improved yield predictions. The proposed correction procedure provides an option for using the daily output of dynamic climate prediction models for impact studies in a manner that preserves any useful predictive information about the timing of rainfall within the season. However, its practical utility for yield forecasting at a long lead-time may be limited by the ability of GCMs to simulate rainfall with a realistic time structure
Automatic interpretation of MSS-LANDSAT data applied to coal refuse site studies in southern Santa Catarina State, Brazil
The coal mining district in southeastern Santa Catarina State is considered one of the most polluted areas of Brazil. The author has identified significant preliminary results on the application of MSS-LANDSAT digital data to monitor the coal refuse areas and its environmental consequences in this region
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Parameter conditioning with a noisy Monte Carlo genetic algorithm for estimating effective soil hydraulic properties from space
The estimation of effective soil hydraulic parameters and their uncertainties is a critical step in all large-scale hydrologic and climatic model applications. Here a scale-dependent (top-down) parameter estimation (inverse modeling) scheme called the noisy Monte Carlo genetic algorithm (NMCGA) was developed and tested for estimating these effective soil hydraulic parameters and their uncertainties. We tested our method using three case studies involving a synthetic pixel (pure and mixed) where all modeling conditions are known, and with actual airborne remote sensing (RS) footprints and a satellite RS footprint. In the synthetic case studies under pure (one soil texture) and mixed-pixel (multiple soil textures) conditions, NMCGA performed well in estimating the effective soil hydraulic parameters even with pixel complexities contributed by various soil types and land management practices (rain-fed/irrigated). With the airborne and satellite remote sensing cases, NMCGA also performed well for estimating effective soil hydraulic properties so that when applied in forward stochastic simulation modeling it can mimic large-scale soil moisture dynamics. The results also suggest a possible scaling down of the effective soil water retention curve (h) at the larger satellite remote sensing pixel compared with the airborne remote sensing pixel. However, it did not generally imply that all effective soil hydraulic parameters should scale down like the soil water retention curve. The reduction of the soil hydraulic parameters was most profound in the saturated soil moisture content ( sat) as we relaxed progressively the soil hydraulic parameter search spaces in our satellite remote sensing studies. Overall, the NMCGA framework was found to be very promising in the inverse modeling of remotely sensed near-surface soil moisture for estimating the effective soil hydraulic parameters and their uncertainties at the remote sensing footprint/climate model grid
Mapping land use changes in the carboniferous region of Santa Catarina, report 2
The techniques applied to MSS-LANDSAT data in the land-use mapping of Criciuma region (Santa Catarina state, Brazil) are presented along with the results of a classification accuracy estimate tested on the resulting map. The MSS-LANDSAT data digital processing involves noise suppression, features selection and a hybrid classifier. The accuracy test is made through comparisons with aerial photographs of sampled points. The utilization of digital processing to map the classes agricultural lands, forest lands and urban areas is recommended, while the coal refuse areas should be mapped visually
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Near-surface soil moisture assimilation for quantifying effective soil hydraulic properties using genetic algorithm: 1. Conceptual modeling
We used a genetic algorithm (GA) to identify soil water retention (h) and hydraulic conductivity K(h) functions by inverting a soil-water-atmosphere-plant (SWAP) model using observed near-surface soil moisture (0-5 cm) as search criterion. Uncertainties of parameter estimates were estimated using multipopulations in GA and considering data and modeling errors. Three hydrologic cases were considered: (1) homogenous free-draining soil column, (2) homogenous soil column with shallow water table, and (3) heterogeneous soil column under free-drainage condition, considering three different rainfall patterns in northern Texas. Results (cases 1 and 2) showed the identifiability of soil hydraulic parameters improving at coarse and fine scales of the soil textural class. Medium-textured soils posed identifiability problems when the soil is dry. Nonlinearity in (h) and K(h) is greater at drier conditions, and some parameters are less sensitive for estimation. Flow regimes controlled by upward fluxes were found less successful, as the information content of observed near-surface data may no longer influence the hydrologic processes in the subsurface. The identifiability of soil hydraulic parameters was found better when the soil profile is predominantly draining. In case 3, top soil layer hydraulic properties were defined using near-surface data alone as criterion. Adding evapotranspiration (ET) improved identification of the second soil layer, although not all parameters were identifiable. Under uncertainties, (h) was found to be well defined while K(h) is more uncertain. Finally, we applied the method to a validation site in Little Washita watershed, Oklahoma, where derived effective soil hydraulic properties closely matched the measured ones at the field site
Surface nutrient regime and bottom hypoxia in Manila Bay during the southwest monsoon
We e amined the surface nutrient regime and hypo ia in Manila Bay, Philippines, during the southwest monsoon along a transect from off Limay, Bataan Peninsula, to Metro Ma nila. The water column showed stratification, with warm, less saline water in the top 10 meters overlying cold, saline bottom water. Hypo ia was present in the bottom waters along the entire transect, and ano ic conditions were observed off Manila. Ammonium concentrations ranged from 6.7 to 40.2 µM, e ceeding those of nitrate and nitrite, both of which were nearly depleted at almost all stations e cept off-Manila, with levels below 0.1 µM. Phosphate varied from 0.1 to 1.9 µM, resulting in a stoichiometrically nitrogen-rich condition at the surface, with N:P ratios ranging from 17.1 to 149.7 and an average of 37.4. This is in contrast to the phosphate-rich con ditions reported during the northeast monsoon period. A plume with high nutrient concentrations and high chlorophyll a was observed off Manila, indicating freshwater inflow from sewage and the Pasig River. Diatoms, including the Skeletonema costatum comple and Chaetoceros spp., were abundant, and these eutrophic conditions likely favored the occurrence of green Noctiluca, while it was not observed during our study.departmental bulletin pape
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Autocalibration of HSPF for Simulation of Streamflow Using a Genetic Algorithm
Hydrologic models are essential to watershed planning and management, particularly in the San Antonio River watershed where competition for scarce water resources is a challenge. As a result, the calibration and validation of hydrologic models are essential steps for their successful application. In this study, we examined the use of a loosely coupled genetic algorithm (GA) as an autocalibration tool for optimization of model parameters for the Hydrologic Simulation Program - Fortran (HSPF), a model frequently used in surface hydrology and water quality modeling. The GA-HSPF model is a more objective and less time-consuming alternative to traditional trial-and-error methods. The objective function was optimized by minimizing the mean absolute error (MAE) between corresponding simulated and observed average daily streamflow in the San Antonio River watershed. The MAE was used to evaluate the fitness of the parameter set in the GA. The calibrated model parameters (LZSN, INFILT, AGWRC, UZSN, DEEPFR, LZETP, and INTFW) were selected based on a sensitivity analysis from a previous study. Goodness-of-fit of the GA calibrated model was evaluated using the Nash-Sutcliffe coefficient of efficiency, MAE, root mean square error, flow duration curves, wavelet analysis, and total volume error. Overall simulation time with 2000 model simulations was 11 days, which can be improved significantly under parallel computing, as GA-HSPF simulations are highly independent. The objective function ceased improvement after approximately 250 simulations, with a minimized MAE of 25.8 m3/s. With the exception of DEEPFR, all optimized model parameter values were within the range cited in the literature. Nash-Sutcliffe coefficients in all simulations were above 0.5, suggesting that the simulated flows were in good agreement with the observed flows. Visual comparison between observed and simulated stream flow using time series and flow duration curves showed that the GA calibrated model was unable to simulate peak flow events accurately, particularly in the 0% to 10% exceedence range. It is hypothesized that the storage-based routing scheme in HSPF limits its ability to predict peak flows in this watershed. Comparison between observed and simulated flows in the wavelet domain indicated that the GA calibrated model was not able to preserve the scale and location of some high frequencies, but the scale and location of lower frequencies were preserved. The cyclic nature of the streamflow in this watershed was more prominent in lower frequencies. While overall flow rates were adequately predicted using a GA-HSPF approach, future work in this watershed needs to focus on multi-objective optimization that optimizes both volumes and peak flows. The GA-HSPF model offers an objective and efficient method for calibration and validation, a useful tool in watershed planning efforts
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