17 research outputs found
Inhibitors of the ATP/ADP antiporter suppress stimulation of mitochondrial respiration and H+ permeability by palmitate and anionic detergents
AbstractThe action of ATP/ADP-antiporter inhibitors upon the uncoupling effect of palmitate, detergents and ‘classical’ uncouplers has been studied. The uncoupling effect was estimated by stimulation of succinate oxidation and of H+ permeability of rat liver mitochondria in the presence of oligomycin. It is shown that carboxyatractylate (CAtr) and pyridoxal 5-phosphate (PLP) suppress the uncoupling induced by palmitate and the anionic detergents SDS and cholate, but do not affect that induced by the cationic detergents CTAB, by the non-ionic detergent Triton X-100, as well as by the ‘classical’ uncouplers FCCP and DNP. The results are discussed in terms of a concept assuming that the ATP/ADP-antiporter facilitates the electrophoretic export of hydrophobic anions from mitochondria
Cellular Adhesion to Carbon Nanotubes-anchored Oligonucleotide: Electro-chemical Detection
In the study a new biosensor contained an oligonucleotide-carbon nanotubes-stearic acid monocrystalline
clusters in its sensitive coating has been developed. The electrochemical characteristics of sensors with
different coatings distinguishing by content of oligonucleotide and cerium ions have been measured. It was
found that the presence of Ce in the coating leads to decrease of sensor capacity changes at measurements
in mediums of different content. The oligonucleotides anchored in sensor coating attenuate cell nonantisense
adhesion to sensor surface.
When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3547
The spectrum features of UHECRs below and surrounding GZK
The energy spectrum of UHECRs is discussed on the basis of the Yakutsk array
database analysis. In the region E=0.1 to 30 EeV the showers are detected under
trigger-500, while at energies above 30 EeV the whole acceptance area for
trigger-1000 is used in order to utilize all the data available in the region
of GZK cutoff.Comment: Invited talk at CRIS2004: GZK and surroundings, Catania, Italy,
31.05.04. To appear in Nucl. Phys. B Proc. Supp
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Energetic particle influence on the Earth's atmosphere
This manuscript gives an up-to-date and comprehensive overview of the effects of energetic particle precipitation (EPP) onto the whole atmosphere, from the lower thermosphere/mesosphere through the stratosphere and troposphere, to the surface. The paper summarizes the different sources and energies of particles, principally
galactic cosmic rays (GCRs), solar energetic particles (SEPs) and energetic electron precipitation (EEP). All the proposed mechanisms by which EPP can affect the atmosphere
are discussed, including chemical changes in the upper atmosphere and lower thermosphere, chemistry-dynamics feedbacks, the global electric circuit and cloud formation. The role of energetic particles in Earth’s atmosphere is a multi-disciplinary problem that requires expertise from a range of scientific backgrounds. To assist with this synergy, summary tables are provided, which are intended to evaluate the level of current knowledge of the effects of energetic particles on processes in the entire atmosphere
Cyclosporin A suppression of uncoupling in liver mitochondria of ground squirrel during arousal from hibernation
AbstractEnergy coupling parameters were studied in liver mitochondria of ground squirrel during arousal from hibernation. It was found that such mitochondria become uncoupled during incubation with phosphate in a salt medium. The uncoupling was revealed by respiration rate increase and membrane potential decrease in the presence of oligomycin. Both effects were reversed by addition of cyclosporin A. Under the same in vitro conditions, mitochondria from aroused (active) animals showed no uncoupling but could be uncoupled by addition of palmitate in the cyclosporin A-sensitive fashion. It is proposed that formation of cyclosporin A-sensitive pores can be involved in urgent heat production in arousing hibernators
Cytotoxic Activity of Salicylic Acid-Containing Drug Models with Ionic and Covalent Binding
Three different types of drug delivery platforms based on imidazolium ionic liquids (ILs) were synthesized in high preparative yields, namely, the models involving (i) ionic binding of drug and IL; (ii) covalent binding of drug and IL; and (iii) dual binding using both ionic and covalent approaches. Seven ionic liquids containing salicylic acid (SA-ILs) in the cation or/and in the anion were prepared, and their cytotoxicity toward the human cell lines CaCo-2 (colorectal adenocarcinoma) and 3215 LS (normal fibroblasts) was evaluated. Cytotoxicity of SA-ILs was significantly higher than that of conventional imidazolium-based ILs and was comparable to the pure salicylic acid. It is important to note that the obtained SA-ILs dissolved in water more readily than salicylic acid, suggesting benefits of possible usage of traditional nonsoluble active pharmaceutical ingredients in an ionic liquid form. © 2015 American Chemical Society
Cytotoxic Activity of Salicylic Acid-Containing Drug Models with Ionic and Covalent Binding
Three different types of drug delivery platforms based on imidazolium ionic liquids (ILs) were synthesized in high preparative yields, namely, the models involving (i) ionic binding of drug and IL; (ii) covalent binding of drug and IL; and (iii) dual binding using both ionic and covalent approaches. Seven ionic liquids containing salicylic acid (SA-ILs) in the cation or/and in the anion were prepared, and their cytotoxicity toward the human cell lines CaCo-2 (colorectal adenocarcinoma) and 3215 LS (normal fibroblasts) was evaluated. Cytotoxicity of SA-ILs was significantly higher than that of conventional imidazolium-based ILs and was comparable to the pure salicylic acid. It is important to note that the obtained SA-ILs dissolved in water more readily than salicylic acid, suggesting benefits of possible usage of traditional nonsoluble active pharmaceutical ingredients in an ionic liquid form. © 2015 American Chemical Society