Análisis de crecimiento y determinación del contenido de fibra de la vaina de cuatro genotipos de habichuela

Se evaluaron en campo cuatro genotipos de habichuela (G9069, G10165, G18722 y la variedad comercial Milenio) con el fin de establecer las diferencias en el contenido de fibra (método de campo y análisis químico de la fibra) y desarrollar el análisis del crecimiento. Con excepción del genotipo 10165 no se observaron diferencias significativas en el contenido de cenizas entre genotipos ni entre muestreos. Durante la tercera semana post-antesis, se alcanzó correlación negativa altamente significativa entre la longitud de la telilla y la fibra detergente neutra (R= – 0.80; R2 = 0.64). Este resultado confiere validez al método clásico de campo para estimar la fibrosidad y la época de cosecha de la vaina. De acuerdo con los resultados del análisis de crecimiento de cada genotipo, es posible estimar tanto la época ideal para la cosecha como la época crítica para la realización de las labores agronómicas. Los genotipos G9069 y G18722 registraron las mejores características para el mercado colombiano en fresco. Abstract Growth analysis and fiber determination in four genotypes of snap bean. In order to establish genotypical differences in fiber content (field method and chemical fiber analysis) and to carry out their growth analysis four snap beans genotypes (G9069, G10165, G18722 and Milenio), were evaluated in field conditions. No significative differences in ashes content and samplings (excepted genotype 10165) were found between the studied genotypes. During the third week after anthesis, highly significative negative correlation coefficient was found between ripped cuticle and NDF (R= – 0.80; R2 = 0.64). This result confirms the validity of the classical method of fiber determination and harvest season of pods in field conditions. According growth analysis results of each genotype, it is possible to estimate the best time for pod harvesting and the critical interval for carry out agronomical practices. Genotypes G9069 y G18722 showed the best characteristics for Colombian market. Key words: Phaseolus vulgaris L, Sheath cality, growth analysis, fiber cuantificatio

Developing country consumers’ acceptance of biofortified foods: a synthesis

The success of biofortified staple crops depends on whether they are accepted and consumed by target populations. In the past 8 years, several studies were undertaken to understand consumers’ acceptance of foods made with biofortified staple crops. Consumer acceptance is measured in terms of their sensory evaluation and economic valuation of biofortified varieties vis-à-vis conventional ones. These studies apply expert sensory panel and hedonic trait analyses methods adopted from food sciences literature, as well as various preference elicitation methods (including experimental auctions, revealed choice experiments, and stated choice experiments) adopted from experimental economics literature. These studies also test the impact of various levers on consumers’ evaluation and valuation for biofortified foods. These levers include (i) nutrition information and the media through which such information is conveyed; (ii) the length and content of nutrition information; (iii) different branding options; (iv) the nature (national or international) of the branding/certification agency that is endorsing the biofortified staple food; and (v) the nature (national or international) of the agency that is delivering the biofortified staple food. This paper brings together evidence on consumer acceptance of biofortified crops on 5 crops across 7 countries in Africa, Asia and Latin America. The results of these studies are expected to aid in the development of biofortified crops that consumers like, as well as in the development of appropriate marketing and consumer awareness or information campaigns to encourage the switch in consumption from traditional staples to biofortified ones

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Secretome of mesenchymal stem cells and its impact on Chronic Obstructive Pulmonary Disease

Chronic obstructive pulmonary disease (COPD) is characterized by irreversible loss of lung function that stem from two mechanisms, inflammation and senescence. Crosstalk between these two mechanisms accelerate the development of COPD, thus targeting these two pathways may offer benefits in the treatment of COPD. Growing amount of evidence have shown that mesenchymal stem cells as a promising candidate for the treatment of COPD. Over the years, many studies conducted to decipher the therapeutic effect of MSC in COPD and the mechanisms involve, in the hope of utilizing these cells as new therapeutic strategy for COPD. However, the cell-based therapy by using the MSC presented with many obstacles including low engraftment at the site of injury, the risk of microvascular occlusion, unwanted differentiation, and also the risk of malignant transformation. Recently, recently researchers begin to look at the possibility of using MSC derived extracellular vesicles as an alternative to MSC. Here we review the effect of MSC and MSC derived EV in modulating inflammation, and senescence in COPD. We also review current treatment and the side effect in COPD, and senolytic drugs, a new therapeutic strategy targeting the senescent cells

Carta esferica desde cabo Oropesa a cabo de Creux

1 manuscrit.56 x 72 c

Estudio de la variabilidad genética en habichuela phaseolus vulgaris l., mediante descriptores morfológicos y bioquímicos

Se cuantificó la variabilidad genética de una muestra de 116 accesiones de habichuela P. vulgaris, cultivadas en centros primarios y secundarios de domesticación. Se evaluaron 18 descriptores morfo-agronómicos asociados con características de la planta, vaina y semilla. Mediante el análisis de las faseolinas utilizando SDS-PAGE se encontraron patrones de bandas de origen andino (T, C y H1) y mesoamericano [S, Sb, CH y H(S+I)]. También se evaluaron ocho sistemas isoenzimáticos polimórficos. En el germoplasma de habichuela hay importante contribución del acervo mesoamericano y las accesiones en algunos centros secundarios de domesticación tuvieron origen y procesos de dispersión diferentes de los del fríjol común en tales zonas. La mayor variabilidad morfológica y el mayor número de accesiones con características deseables para el mercado fresco se encontró en el grupo mesoamericano. Se detectó mayor número de genotipos híbridos entre acervos cuando se utilizaron simultáneamente los tres descriptores, lo cual indica una estructura genética compleja que podría deberse al efecto de los factores ambientales propios de la zona templada sobre sus patrones reproductivos. La diversidad total medida con los tres descriptores fue similar a la registrada en fríjol común. Sin embargo, la estructura poblacional encontrada por otros autores en el fríjol común es diferente de la observada en este estudio. Palabras claves: Variabilidad, descriptores morfológicos, isoenzimas, proteínas de semilla, acervos genéticos. ABSTRACT Genetic variability of 116 accesions of Phaseolus vulgaris showing snap beans characteristics coming from primary and secondary centers of domestication, were studied using eighteen morphological descriptors to characterize pods and seeds, SDS-PAGE analysis of seed phaseolins and eight isozyme systems. Higher morphological diversity and best pod marketing characteristics were found at Andean accessions. After SDS-PAGE analysis Andean (T, C, and H) and Mesoamerican [S, Sb, CH and H(S+1)] types of phaseolin were detected in the sample, suggesting a higher contribution of the Mesoamerican gene pool to the genotype of snap beans. Also it was observed that, at the same zones of collection in some secondary centers, snap beans were originated and dispersed differently as compared to dry beans. A higher number of hybrid genotypes were detected among genetic pools when the three markers were used. Results seems to indicate that snap beans developed under environmental effects of the temperate zones, have a more complex genetic structure and higher rates of allogamy than those observed at primary centers of domestication. The results obtained using the three descriptors show that total genetic diversity for snap beans is similar to that of dry beans, but the snap beans population structure observed in this study is different to that reported for dry beans. Key words: Genetic variability, morphological descriptors, isozymes, seed protein, genetic pool

Estudio de la variabilidad genética en habichuela Phaseolus vulgaris L., mediante descriptores morfológicos y bioquímicos

Se cuantificó la variabilidad genética de una muestra de 116 accesiones de habichuela P. vulgaris, cultivadas en centros primarios y secundarios de domesticación. Se evaluaron 18 descriptores morfo-agronómicos asociados con características de la planta, vaina y semilla. Mediante el análisis de las faseolinas utilizando SDS-PAGE se encontraron patrones de bandas de origen andino (T, C y H1) y mesoamericano [S, Sb, CH y H(S+I)]. También se evaluaron ocho sistemas isoenzimáticos polimórficos. En el germoplasma de habichuela hay importante contribución del acervo mesoamericano y las accesiones en algunos centros secundarios de domesticación tuvieron origen y procesos de dispersión diferentes de los del fríjol común en tales zonas. La mayor variabilidad morfológica y el mayor número de accesiones con características deseables para el mercado fresco se encontró en el grupo mesoamericano. Se detectó mayor número de genotipos híbridos entre acervos cuando se utilizaron simultáneamente los tres descriptores, lo cual indica una estructura genética compleja que podría deberse al efecto de los factores ambientales propios de la zona templada sobre sus patrones reproductivos. La diversidad total medida con los tres descriptores fue similar a la registrada en fríjol común. Sin embargo, la estructura poblacional encontrada por otros autores en el fríjol común es diferente de la observada en este estudio.
 
 Palabras claves: Variabilidad, descriptores morfológicos, isoenzimas, proteínas de semilla, acervos genéticos.
 
 ABSTRACT
 
 Genetic variability of 116 accesions of Phaseolus vulgaris showing snap beans characteristics coming from primary and secondary centers of domestication, were studied using eighteen morphological descriptors to characterize pods and seeds, SDS-PAGE analysis of seed phaseolins and eight isozyme systems. Higher morphological diversity and best pod marketing characteristics were found at Andean accessions. After SDS-PAGE analysis Andean (T, C, and H) and Mesoamerican [S, Sb, CH and H(S+1)] types of phaseolin were detected in the sample, suggesting a higher contribution of the Mesoamerican gene pool to the genotype of snap beans. Also it was observed that, at the same zones of collection in some secondary centers, snap beans were originated and dispersed differently as compared to dry beans. A higher number of hybrid genotypes were detected among genetic pools when the three markers were used. Results seems to indicate that snap beans developed under environmental effects of the temperate zones, have a more complex genetic structure and higher rates of allogamy than those observed at primary centers of domestication. The results obtained using the three descriptors show that total genetic diversity for snap beans is similar to that of dry beans, but the snap beans population structure observed in this study is different to that reported for dry beans.
 
 Key words: Genetic variability, morphological descriptors, isozymes, seed protein, genetic pool

Análisis de crecimiento y determinación del contenido de fibra de la vaina de cuatro genotipos de habichuela

Se evaluaron en campo cuatro genotipos de habichuela (G9069, G10165, G18722 y la variedad comercial Milenio) con el fin de establecer las diferencias en el contenido de fibra (método de campo y análisis químico de la fibra) y desarrollar el análisis del crecimiento. Con excepción del genotipo 10165 no se observaron diferencias significativas en el contenido de cenizas entre genotipos ni entre muestreos. Durante la tercera semana post-antesis, se alcanzó correlación negativa altamente significativa entre la longitud de la telilla y la fibra detergente neutra (R= – 0.80; R2 = 0.64). Este resultado confiere validez al método clásico de campo para estimar la fibrosidad y la época de cosecha de la vaina. De acuerdo con los resultados del análisis de crecimiento de cada genotipo, es posible estimar tanto la época ideal para la cosecha como la época crítica para la realización de las labores agronómicas. Los genotipos G9069 y G18722 registraron las mejores características para el mercado colombiano en fresco.
 
 Abstract
 
 Growth analysis and fiber determination in four genotypes of snap bean. In order to establish genotypical differences in fiber content (field method and chemical fiber analysis) and to carry out their growth analysis four snap beans genotypes (G9069, G10165, G18722 and Milenio), were evaluated in field conditions. No significative differences in ashes content and samplings (excepted genotype 10165) were found between the studied genotypes. During the third week after anthesis, highly significative negative correlation coefficient was found between ripped cuticle and NDF (R= – 0.80; R2 = 0.64). This result confirms the validity of the classical method of fiber determination and harvest season of pods in field conditions. According growth analysis results of each genotype, it is possible to estimate the best time for pod harvesting and the critical interval for carry out agronomical practices. Genotypes G9069 y G18722 showed the best characteristics for Colombian market.
 
 Key words: Phaseolus vulgaris L, Sheath cality, growth analysis, fiber cuantificatio