An efficient protocol to perform genetic traceability of tissue and foods from Geoffroea decorticans

The quality of a DNA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans Burkart (chanar) is a species that has nutritional and pharmacological potential. However, an effective method of DNA extraction capable of facilitating population studies and food genetic traceability has not been studied yet. The objective of the present work was to evaluate four methods of DNA extraction from leaves and chanar-based foods. The methods were evaluated based on yield, DNA purity, and molecular markers. The CCI-P (CTAB/Chloroform-Isoamylalcohol/pellet) method showed the highest yield of DNA obtained from leaves. However, the CPCI-SC (CTAB/Phenol-Chloroform-Isoamylalcohol/silica-column) method was the only one that resulted in acceptable DNA quality with both parameters (A260/A280 and A260/A230). The leaf DNA obtained with this method showed a greater amount of fragments with RAPD, and an acceptable amount of fragments with ISSR. On the other hand, the CCI-P method showed a higher yield of DNA from arrope de chanar (syrup). However, the CPCI-SC method was the only one that had relatively better DNA quality, which allowed the amplification of molecular markers. Regarding chanar flour, the CPCI-SC method showed the highest yield, DNA quality and good amplification with molecular markers. Therefore, the CPCI-SC extraction method is efficient for obtaining DNA from different matrices, and can support studies for a possible designation of origin of chanar-based foods

Incremental and Modular Context-sensitive Analysis

Context-sensitive global analysis of large code bases can be expensive, which can make its use impractical during software development. However, there are many situations in which modifications are small and isolated within a few components, and it is desirable to reuse as much as possible previous analysis results. This has been achieved to date through incremental global analysis fixpoint algorithms that achieve cost reductions at fine levels of granularity, such as changes in program lines. However, these fine-grained techniques are not directly applicable to modular programs, nor are they designed to take advantage of modular structures. This paper describes, implements, and evaluates an algorithm that performs efficient context-sensitive analysis incrementally on modular partitions of programs. The experimental results show that the proposed modular algorithm shows significant improvements, in both time and memory consumption, when compared to existing non-modular, fine-grain incremental analysis techniques. Furthermore, thanks to the proposed inter-modular propagation of analysis information, our algorithm also outperforms traditional modular analysis even when analyzing from scratch.Comment: 56 pages, 27 figures. To be published in Theory and Practice of Logic Programming. v3 corresponds to the extended version of the ICLP2018 Technical Communication. v4 is the revised version submitted to Theory and Practice of Logic Programming. v5 (this one) is the final author version to be published in TPL

The peculiar molecular envelope around the post-AGB star IRAS 08544--4431

Circumbinary disks have been hypothesized to exist around a number of binary post-AGB stars. Although most of the circumbinary disks have been inferred through the near IR excess, a few of them are strong emitters of molecular emission. Here we present high angular resolution observations of the emission of $^{12}$CO and its isotopomer $^{13}$CO J=2--1 line from the circumstellar envelope around the binary post-AGB star IRAS 08544$-$4431, which is one of the most prominent members of this class of objects. We find that the envelope is resolved in our observations and two separate components can be identified: (a) a central extended and strong component with very narrow linewidth between 2 - 6 \kms; (b) a weak bipolar outflow with expansion velocity up to 8 \kms. The central compact component possesses low and variable $^{12}$CO/$^{13}$CO J=2--1 line ratio, indicating optically thick emission of the main isotope. We estimate a molecular gas mass of 0.0047 M$_\odot$ for this component based on the optically thinner $^{13}$CO J=2--1 line. We discuss the relation of the molecular envelope and the circumbinary disk inferred from near IR excess and compare with other known cases where the distribution of molecular gas has been imaged at high angular resolution.Comment: 14 pages, 4 figures. Accepted for publication in Astrophysical Journa

Urban monuments

Surviving in poverty in an urban environment is hard work. Fear of not having basic needs creates desperation, and not being able to improve upon these conditions can test ones patience and faith in others. The simple routine of daily life can be monumental. Small achievements can be huge moments for some and irrelevant to others. One learns to appreciate and hate what one has. Urban Monuments comes from a struggle to understand what painting is to me and the relationship it has with this idea. Working hard to better ones self or ones situation is a noble cause but it is by no means a guarantee. These urban monuments commemorate the struggle to survive in an unforgiving environment

Water vapor and silicon monoxide maser observations in the protoplanetary nebula OH 231.8+4

OH 231.8+4.2 is a well studied preplanetary nebula (pPN) around a binary stellar system that shows a remarkable bipolar outflow. To study the structure and kinematics of the inner 10-80 AU nebular regions we performed high-resolution observations of the H$_2$O 6$_{1,6}$--5$_{2,3}$ and $^{28}$SiO $v$=2, $J$=1--0 maser emissions with the Very Long Baseline Array. The absolute position of both emission distributions were recovered using the phase referencing technique, and accurately registered in HST optical images. H$_2$O maser clumps are found to be distributed in two areas of 20 mas in size spatially displaced by $\sim$60 milli-arcseconds along an axis oriented nearly north-south. SiO masers are tentatively found to be placed between the two H$_2$O maser emitting regions, probably indicating the position of the Mira component of the system. The SiO maser emission traces an inner equatorial component with a diameter of 12 AU, probably a disk rotating around the M-type star. Outwards, we detect in the H$_2$O data a pair of polar caps, separated by 80 AU. We believe that the inner regions of the nebula probably have been altered by the presence of the companion, leading to an equator-to-pole density contrast that may explain the lack of H$_2$O masers and strong SiO maser emission in the denser, equatorial regions.Comment: 5 pages, 1 figure, A&A accepte

Behavioural problems during the childhood: theorical approach through a case study

En fænomenologisk og antropologisk analyse af legen som et universelt, eksistentielt rum for mennesket.Presence – An attempt at an easy definition of playIn this article a simple definition of play has been attempted within the framework of phenomenological anthropology. When the process is seen as more serious than the result, we play. When, on the contrary, the result is the more important, we work. To express it in simple formulas in which P is process and R, result:P/R > 1 = Play P/R < 1 = WorkThe definition is discussed in relation to a number of approsing rations which normally provoke reflexive efforts: play vs. seriousness; play vs. labour; play vs. reality; play vs. war; play vs. sport; play vs. ritual; play as symbol and metaphor.Further, the phenomenological concept of play is contrasted to the pedagogical concept, in an analysis of the instrumental use of play vs. play as a meaningful practise in itself.Finally, reference is made to W.H. Auden’s view of the aesthetic process as a dual act involving sin and the search for redemption

A pilot search for mm-wavelength recombination lines from emerging ionized winds in pre-planetary nebulae candidates

We report the results from a pilot search for radio recombination line (RRL) emission at millimeter wavelengths in a small sample of pre-planetary nebulae (pPNe) and young PNe (yPNe) with emerging central ionized regions. Observations of the H30\alpha, H31a, H39a, H41a, H48b, H49b, H51b, and H55g lines at 1 and 3mm have been performed with the IRAM 30 m radio telescope. These lines are excellent probes of the dense inner (<~150 au) and heavily obscured regions of these objects, where the yet unknown agents for PN-shaping originate. We detected mm-RRLs in three objects: CRL 618, MWC 922, and M 2-9. For CRL 618, the only pPN with previous published detections of H41a, H35a, and H30a emission, we find significant changes in the line profiles indicating that current observations are probing regions of the ionized wind with larger expansion velocities and mass-loss rate than ~29 years ago. In the case of MWC 922, we observe a drastic transition from single-peaked profiles at 3mm to double-peaked profiles at 1mm, which is consistent with maser amplification of the highest frequency lines; the observed line profiles are compatible with rotation and expansion of the ionized gas, probably arranged in a disk+wind system around a ~5-10 Msun central mass. In M 2-9, the mm-RRL emission appears to be tracing a recent mass outburst by one of the stars of the central binary system. We present the results from non-LTE line and continuum radiative transfer models, which enables us to constrain the structure, kinematics, and physical conditions (electron temperature and density) of the ionized cores of our sample. (abridged). We deduce mass-loss rates of ~1e-6-1e-7 Msun/yr, which are significantly higher than the values adopted by stellar evolution models currently in use and would result in a transition from the asymptotic giant branch to the PN phase faster than hitherto assumed.Comment: Accepted by Astronomy and Astrophysics. 28 pages, including figure

Torsion induces Gravity

In this work the Poincare-Chern Simons and Anti de Sitter Chern Simons gravities are studied. For both a solution that can be casted as a black hole with manifest torsion is found. Those solutions resemble Schwarzschild and Schwarzschild-AdS solutions respectively.Comment: 4 pages, RevTe