RNase T1 mimicking artificial ribonuclease

Recently, artificial ribonucleases (aRNases)—conjugates of oligodeoxyribonucleotides and peptide (LR)4-G-amide—were designed and assessed in terms of the activity and specificity of RNA cleavage. The conjugates were shown to cleave RNA at Pyr-A and G–X sequences. Variations of oligonucleotide length and sequence, peptide and linker structure led to the development of conjugates exhibiting G–X cleavage specificity only. The most efficient catalyst is built of nonadeoxyribonucleotide of unique sequence and peptide (LR)4-G-NH2 connected by the linker of three abasic deoxyribonucleotides (conjugate pep-9). Investigation of the cleavage specificity of conjugate pep-9 showed that the compound is the first single-stranded guanine-specific aRNase, which mimics RNase T1. Rate enhancement of RNA cleavage at G–X linkages catalysed by pep-9 is 108 compared to non-catalysed reaction, pep-9 cleaves these linkages only 105-fold less efficiently than RNase T1 (kcat_RNase T1/kcat_pep-9 = 105)

Assessment of environmental and economic efficiency of iron ore breaking technology using emulsion explosives

Methodology for calculating parameters of drilling and blasting operations for stoping works in mines of Kryvorozhskiy basin and PJSC «Zaporizhskiy iron-ore plant» has been improved with the help of established coefficient of relative capacity for the Ukrainit-PM-2B explosive. A new technology of stoping operations for ore breaking by square-chamber methods at deposit thickness more than 5 m is proposed which assumes usage of emulsion explosives and downward drilling of production hole rings in the direction of underlying drilling horizons. Ecological and economical effectiveness of the proposed ore breaking technology implemented in the extraction chambers was estimated. Regularities of harmful substances hazard index changing were established depending on distance to the emission point when trotyl-contained and emulsion explosives are used. Implementation of the proposed technology allows decreasing prime-cost of 1 ton of ore by 15 % per one extraction unit

Accounting agricultural business from scratch: management accounting, decision making, analysis and monitoring of business processes

The article reveals the key points of accounting and management actions when creating an agricultural business from scratch for growing seedlings of vegetables in a greenhouse. The main economic instruments for creating an agricultural business for growing vegetable seedlings in a greenhouse in Russia are: planning, management, accounting, organization, analysis and monitoring of business processes. Today, the state is increasingly focusing on the development of the industry, helping beginners and existing farmers, allocating land at preferential rates, subsidizing interest rates on loans and providing grants for the development of greenhouses. However, the main problem holding back the development of the industry remains high utility tariffs. One of the main conditions for obtaining reliable management information on business value is a clear definition of the costs incurred to create a business from scratch and its further sustainable development. The aim of the study is to develop management accounting criteria for organizing a business from scratch. The subject of the study is management accounting as an integrated mechanism in terms of creating and developing an agricultural business from scratch. In accordance with this goal, the main task was determined: to develop managerial decisions to create an agricultural business (greenhouse) for growing seedlings of vegetables for its implementation in the Udmurt Republic. It is concluded that achieving high profits is possible with a competent managerial approach, taking into account the further expansion and cultivation of other seedling crops, despite the complexity and specificity of this type of activity

Possible effect of extreme solar energetic particle event of 20 January 2005 on polar stratospheric aerosols: direct observational evidence

Energetic cosmic rays are the main source of ionization of the low-middle atmosphere, leading to associated changes in atmospheric properties. Via the hypothetical influence of ionization on aerosol growth and facilitated formation of clouds, this may be an important indirect link relating solar variability to climate. This effect is highly debated, however, since the proposed theoretical mechanisms still remain illusive and qualitative, and observational evidence is inconclusive and controversial. Therefore, important questions regarding the existence and magnitude of the effect, and particularly the fraction of aerosol particles that can form and grow, are still open. Here we present empirical evidence of the possible effect caused by cosmic rays upon polar stratospheric aerosols, based on a case study of an extreme solar energetic particle (SEP) event of 20 January 2005. Using aerosol data obtained over polar regions from different satellites with optical instruments that were operating during January 2005, such as the Stratospheric Aerosol and Gas Experiment III (SAGE III), and Optical Spectrograph and Infrared Imaging System (OSIRIS), we found a significant simultaneous change in aerosol properties in both the Southern and Northern Polar regions in temporal association with the SEP event. We speculate that ionization of the atmosphere, which was abnormally high in the lower stratosphere during the extreme SEP event, might have led to formation of new particles and/or growth of preexisting ultrafine particles in the polar stratospheric region. However, a detailed interpretation of the effect is left for subsequent studies. This is the first time high vertical resolution measurements have been used to discuss possible production of stratospheric aerosols under the influence of cosmic ray induced ionization. The observed effect is marginally detectable for the analyzed severe SEP event and can be undetectable for the majority of weak-moderate events. The present interpretation serves as a conservative upper limit of solar energetic particle effect upon polar stratospheric aerosols