Space weather effects on drilling accuracy in the North Sea

The oil industry uses geomagnetic field information to aid directional drilling operations when drilling for oil and gas offshore. These operations involve continuous monitoring of the azimuth and inclination of the well path to ensure the target is reached and, for safety reasons, to avoid collisions with existing wells. Although the most accurate method of achieving this is through a gyroscopic survey, this can be time consuming and expensive. An alternative method is a magnetic survey, where measurements while drilling (MWD) are made along the well by magnetometers housed in a tool within the drill string. These MWD magnetic surveys require estimates of the Earth’s magnetic field at the drilling location to correct the downhole magnetometer readings. The most accurate corrections are obtained if all sources of the Earth’s magnetic field are considered. Estimates of the main field generated in the core and the local crustal field can be obtained using mathematical models derived from suitable data sets. In order to quantify the external field, an analysis of UK observatory data from 1983 to 2004 has been carried out. By accounting for the external field, the directional error associated with estimated field values at a mid-latitude oil well (55 N) in the North Sea is shown to be reduced by the order of 20%. This improvement varies with latitude, local time, season and phase of the geomagnetic activity cycle. By accounting for all sources of the field, using a technique called Interpolation In-Field Referencing (IIFR), directional drillers have access to data from a “virtual” magnetic observatory at the drill site. This leads to an error reduction in positional accuracy that is close to matching that of the gyroscopic survey method and provides a valuable independent technique for quality control purposes

Expanding the scope of ligand substitution from [M(S2C2Ph2] (M = Ni2+, Pd2+, Pt2+) to afford new heteroleptic dithiolene complexes

The scope of direct substitution of the dithiolene ligand from [M(S2C2Ph2)2] [M = Ni2+ (1), Pd2+ (2), Pt2+ (3)] to produce heteroleptic species [M(S2C2Ph2)2Ln] (n = 1, 2) has been broadened to include isonitriles and dithiooxamides in addition to phosphines and diimines. Collective observations regarding ligands that cleanly produce [M(S2C2Ph2)Ln], do not react at all, or lead to ill-defined decomposition identify soft σ donors as the ligand type capable of dithiolene substitution. Substitution of MeNC from [Ni(S2C2Ph2)(CNMe)2] by L provides access to a variety of heteroleptic dithiolene complexes not accessible from 1. Substitution of a dithiolene ligand from 1 involves net redox disproportionation of the ligands from radical monoanions, –S•SC2Ph2, to enedithiolate and dithione, the latter of which is an enhanced leaving group that is subject to further irreversible reactions

Sensitivity and specificity of a competitive enzyme immunoassay in the serodiagnosis of bovine brucellosis

O trabalho teve por objetivo avaliar a sensibilidade e a especificidade de um teste imunoenzimático competitivo, empregando como conjugado os anticorpos monoclonais BM-38 e BM-40, no diagnóstico sorológico da brucelose bovina. Foram examinados 74 soros de bovinos dos quais havia sido isolada Brucella abortus e 2.118 soros de bovinos procedentes de rebanhos livres de brucelose e que apresentaram resultado negativo quando submetidos ao teste Rosa Bengala. O teste imunoenzimático competitivo, usando qualquer dos dois conjugados, foi capaz de revelar a presença de anticorpos contra o lipopolissacáride bacteriano em todos os soros de bovinos infectados, o que resulta em uma sensibilidade de 100%. A especificidade do teste usando o conjugado BM-38 foi de 98.82% e usando o conjugado BM-40 foi de 99,95%. Estes resultados indicam que o teste imunoenzimático competitivo, principalmente ao se empregar o conjugado BM-40, consiste em um método bastante útil para ser usado como teste confirmatório no diagnóstico sorológico da brucelose bovina.The purpose of this work was to evaluate the sensitivity and the specificity of a competitive enzyme immunoassay, using as conjugate the monoclonal antibodies BM-38 and BM-40, in the serodiagnosis of bovine brucellosis. Seventy-four sera from culture-positive cattle and 2,118 cattle sera from herds free from brucellosis and negative to the Rose Bengal plate test were examined. The competitive enzyme immunoassay, using any of the two conjugates, was able to reveal the presence of antibodies to Brucella lipopolysaccharide in all of the 74 sera of the infected cattle, resulting in a sensitivity of 100%. The specificity of the test using the conjugate BM-38 was 98.82% and using the conjugate BM-40 was 99.95%. These results indicated that the competitive enzyme immunoassay, mainly when using the conjugate BM-40, consists in a technique very useful in the confirmation of the serological diagnosis of bovine brucellosis

Cross-tolerance and cross-talk in the cold: relating low temperatures to desiccation and immune stress in insects.

Multiple stressors, both abiotic and biotic, often are experienced simultaneously by organisms in nature. Responses to these stressors may share signaling pathways ( cross-talk ) or protective mechanisms ( cross-tolerance ). Temperate and polar insects that must survive the winter experience low temperatures accompanied by additional abiotic stressors, such as low availability of water. Cold and desiccation have many similar effects at a cellular level, and we present evidence that the cellular mechanisms that protect against cold stress also protect against desiccation, and that the responses to cold and dehydration likely evolved as cross-tolerance. By contrast, there are several lines of evidence suggesting that low temperature stress elicits an upregulation of immune responses in insects (and vice versa). Because there is little mechanistic overlap between cold stress and immune stress at the cellular level, we suggest that this is cross-talk. Both cross-talk and cross-tolerance may be adaptive and likely evolved in response to synchronous stressors; however, we suggest that cross-talk and cross-tolerance may lead to different responses to changes in the timing and severity of multiple stress interactions in a changing world. We present a framework describing the potentially different responses of cross-tolerance and cross-talk to a changing environment and describe the nature of these impacts using interaction of cold-desiccation and cold-immunity in overwintering insects as an example

Roles of domain-general auditory processing in spoken second-language vocabulary attainment in adulthood

Recently, scholars have begun to explore the hypothesis that individual differences in domain-general auditory perception, which has been identified as an anchor of L1 acquisition, could explain some variance in postpubertal L2 learners’ segmental and suprasegmental learning in immersive settings. The current study set out to examine the generalizability of the topic to the acquisition of higher-level linguistic production skills—that is the appropriate use of diverse, rich, and abstract vocabulary. The speech of 100 Polish-English bilinguals was elicited using an interview task, submitted to corpus-/rater-based linguistic analyses, and linked to their ability to discriminate sounds based on individual acoustic dimensions (pitch, duration, and amplitude). According to the results, those who attained more advanced L2 lexical proficiency demonstrated not only more relevant experience (extensive immersion and earlier age of arrival), but also more precise auditory perception ability

BRIDGING DIGITAL AND ANALOG DOCUMENTATION FOR THE PRESERVATION OF ANCIENT PUEBLOAN SITES

The Four Corners region, where Colorado, Utah, New Mexico, and Arizona meet, is the location of one of the most significant archaeological sites in the United States, the Lowry Pueblo in the Canyon of the Ancients National Monument. This case study examines two aspects of the documentation process for the Lowry Pueblo. First, a collaborative model of multiple stakeholders dedicated to an integrated digital and analog documentation approach. Second, the method for determining how digital and analog techniques were used from site preparation to the final submittal of Historic Architectural Building Survey (HABS) drawings to the Library of Congress. This integrated documentation approach used for the Lowry Pueblo is part of a strategy informed by predicted future human impacts on these rare ancient resources and fulfilling a unique tribal request for minimally impactful management. The approach allows natural erosional processes to continue unabated at less visited remote “backcountry” cultural sites, and for proactive stabilization to occur at heavily visited publicly accessible "front country" areas. The combined documentation methods used allowed for creation of highly detailed models, digital applications, and nuanced, aesthetic HABS drawings of the stunning 1,000-year-old cultural resources. The outcomes contribute to informed decision making for future analysis, stabilization assessment, resource interpretation, archiving, and the dissemination of information for public benefit, while deepening an understanding of the history and people that inhabited the land

Developing, Analyzing and Sharing Multivariate Datasets: Individual Differences in L2 Learning Revisited

Following the trends established in psychology and emerging in L2 research, we explain our support for an Open Science approach in this paper (i.e., developing, analyzing and sharing datasets) as a way to answer controversial and complex questions in applied linguistics. We illustrate this with a focus on a frequently debated question, what underlies individual differences in the dynamic system of post-pubertal L2 speech learning? We provide a detailed description of our dataset which consists of spontaneous speech samples, elicited from 110 late L2 speakers in the UK with diverse linguistic, experiential and sociopsychological backgrounds, rated by ten L1 English listeners for comprehensibility and nativelikeness. We explain how we examined the source of individual differences by linking different levels of L2 speech performance to a range of learner-extrinsic and intrinsic variables related to first language backgrounds, age, experience, motivation, awareness, and attitudes using a series of factor and Bayesian mixed-effects ordinal regression analyses. We conclude with a range of suggestions for the fields of applied linguistics and SLA, including the use of Bayesian methods in analyzing multivariate, multifactorial data of this kind, and advocating for publicly available datasets. In keeping with recommendations for increasing openness of the field, we invite readers to rethink and redo our analyses and interpretations from multiple angles by making our dataset and coding publicly available as part of our 40th anniversary ARAL article

Heterologously-expressed and Liposome-reconstituted Human Transient Receptor Potential Melastatin 4 Channel (TRPM4) is a Functional Tetramer

Mutation, irregular expression and sustained activation of the Transient Receptor Potential Channel, type Melastatin 4 (TRPM4), have been linked to various cardiovascular diseases. However, much remains unknown about the structure of this important ion channel. Here, we have purified a heterologously expressed TRPM4-eGFP fusion protein and investigated the oligomeric state of TRPM4-eGFP in detergent micelles using crosslinking, native gel electrophoresis, multi-angle laser light scattering and electron microscopy. Our data indicate that TRPM4 is tetrameric, like other TRP channels studied to date. Furthermore, the functionality of liposome reconstituted TRPM4-eGFP was examined using electrophysiology. Single-channel recordings from TRPM4-eGFP proteoliposomes showed inhibition of the channel using Flufenamic acid, a well-established inhibitor of TRPM4, suggesting that the channels are functional upon reconstitution. Our characterisation of the oligomeric structure of TRPM4 and the ability to reconstitute functional channels in liposomes should facilitate future studies into the structure, function and pharmacology of this therapeutically relevant channel

Identification and initial response to children\u27s exposure to intimate partner violence: A qualitative synthesis of the perspectives of children, mothers and professionals

Objectives: To synthesise evidence on the acceptable identification and initial response to children\u27s exposure to intimate partner violence (IPV) from the perspectives of providers and recipients of healthcare and social services. Design: We conducted a thematic synthesis of qualitative research, appraised the included studies with the modified Critical Appraisal Skills Programme checklist and undertook a sensitivity analysis of the studies scored above 15. Data sources: We searched eight electronic databases, checked references and citations and contacted authors of the included studies. Eligibility criteria: We included qualitative studies with children, parents and providers of healthcare or social services about their experiences of identification or initial responses to children\u27s exposure to IPV. Papers that have not been peer-reviewed were excluded as well as non-English papers. Results: Searches identified 2039 records; 11 studies met inclusion criteria. Integrated perspectives of 42 children, 212 mothers and 251 professionals showed that sufficient training and support for professionals, good patient-professional relationship and supportive environment for patient/clients need to be in place before enquiry/disclosure of children\u27s exposure to IPV should occur. Providers and recipients of care favour a phased enquiry about IPV initiated by healthcare professionals, which focuses on \u27safety at home\u27 and is integrated into the context of the consultation or visit. Participants agreed that an acceptable initial response prioritises child safety and includes emotional support, education about IPV and signposting to IPV services. Participants had conflicting perspectives on what constitutes acceptable engagement with children and management of safety. Sensitivity analysis produced similar results. Conclusions: Healthcare and social service professionals should receive sufficient training and ongoing individual and system-level support to provide acceptable identification of and initial response to children\u27s exposure to IPV. Ideal identification and responses should use a phased approach to enquiry and the WHO Listen, Inquire about needs and concerns, Validate, Enhance safety and Support principles integrated into a trauma-informed and violence-informed model of care

Understanding Urban Demand for Wild Meat in Vietnam: Implications for Conservation Actions

Vietnam is a significant consumer of wildlife, particularly wild meat, in urban restaurant settings. To meet this demand, poaching of wildlife is widespread, threatening regional and international biodiversity. Previous interventions to tackle illegal and potentially unsustainable consumption of wild meat in Vietnam have generally focused on limiting supply. While critical, they have been impeded by a lack of resources, the presence of increasingly organised criminal networks and corruption. Attention is, therefore, turning to the consumer, but a paucity of research investigating consumer demand for wild meat will impede the creation of effective consumer-centred interventions. Here we used a mixed-methods research approach comprising a hypothetical choice modelling survey and qualitative interviews to explore the drivers of wild meat consumption and consumer preferences among residents of Ho Chi Minh City, Vietnam. Our findings indicate that demand for wild meat is heterogeneous and highly context specific. Wild-sourced, rare, and expensive wild meat-types are eaten by those situated towards the top of the societal hierarchy to convey wealth and status and are commonly consumed in lucrative business contexts. Cheaper, legal and farmed substitutes for wild-sourced meats are also consumed, but typically in more casual consumption or social drinking settings. We explore the implications of our results for current conservation interventions in Vietnam that attempt to tackle illegal and potentially unsustainable trade in and consumption of wild meat and detail how our research informs future consumer-centric conservation actions