Effects of in vitro metabolism of a broccoli leachate, glucosinolates and S-methylcysteine sulphoxide on the human faecal microbiome

Purpose: Brassica are an important food source worldwide and are characterised by the presence of compounds called glucosinolates. Studies indicate that the glucosinolate derived bioactive metabolite sulphoraphane can elicit chemoprotective benefits on human cells. Glucosinolates can be metabolised in vivo by members of the human gut microbiome, although the prevalence of this activity is unclear. Brassica and Allium plants also contain S-methylcysteine sulphoxide (SMCSO), that may provide additional health benefits but its metabolism by gut bacteria is not fully understood. Methods: We examined the effects of a broccoli leachate (BL) on the composition and function of human faecal microbiomes of five different participants under in vitro conditions. Bacterial isolates from these communities were then tested for their ability to metabolise glucosinolates and SMCSO. Results: Microbial communities cultured in vitro in BL media were observed to have enhanced growth of lactic acid bacteria, such as lactobacilli, with a corresponding increase in the levels of lactate and short-chain fatty acids. Members of Escherichia isolated from these faecal communities were found to bioconvert glucosinolates and SMCSO to their reduced analogues. Conclusion: This study uses a broccoli leachate to investigate the bacterial-mediated bioconversion of glucosinolates and SMCSO, which may lead to further products with additional health benefits to the host. We believe that this is the first study that shows the reduction of the dietary compound S-methylcysteine sulphoxide by bacteria isolated from human faeces

Endophytic fungi from the roots of horseradish (Armoracia rusticana) and their interactions with the defensive metabolites of the glucosinolate myrosinase – isothiocyanate system

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Raw dataset for bacterial exopolysaccharide production using riceberry broken rice as precursors by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This raw dataset reflects an analysis of the antioxidant and antibacterial activity of exopolysaccharide (EPS) produced by seven Bacillus spp. isolated from Thai milk kefir [1] on a substrate of riceberry broken rice. Riceberry broken rice was analyzed for its moisture, ash, fat, protein, fiber, starch, sugar, hemicellulose, and cellulose plus lignin content when used as an EPS-producing substrate. After 3 days of fermentation, dataset on the total plate count, the reducing sugar content, the pH of the culture, and the fresh and dry weight of the EPS was recorded. In response to the EPS produced by various Bacillus spp., the total phenolic content, total flavonoid content, antimicrobial activity against Streptococcus agalactiae and Staphylococcus aureus, and hydroxyl radical scavenging activity were collected. Agar disk diffusion, spectrophotometry for antioxidant activity and bioactive content, proximate analysis, the dinitrosalicylic acid method, pH meter readings, the total plate count via serial dilutions, and the spread plate technique were the primary techniques used

Data on bacterial exopolysaccharide production using riceberry broken rice as precursors by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This data represented the evaluation of exopolysaccharide (EPS) production by Bacillus spp. isolated from Thai milk kefir [1] using riceberry broken rice as substrate and evaluating its antioxidant and antimicrobial activities. In Thailand, riceberry broken rice is an agricultural residue of several million tons per year from Thailand’s rice mill industry that is mostly not efficiently used or bio-transformed into more value-added bioproducts. Certain bacterial exopolysaccharides exhibit antioxidant and antimicrobial activities, which make them useful in the food, feed, pharmaceutical, and cosmetic industries. Chemical composition analysis of riceberry broken rice as an EPS substrate provided moisture, ash, fat, protein, fiber, starch, sugar, hemicellulose, and cellulose plus lignin content data. EPS production content in fresh and dry weight, pH of culture, reducing sugar content, and total plate count of bacteria were reported after 3-day fermentation. Antioxidant activities and bioactive content, including hydroxyl radical scavenging activity, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, ferric reducing antioxidant power, total phenolic content, total flavonoid content, and antimicrobial activity towards two bacterial pathogens of Nile tilapia, Streptococcus agalactiae, and Staphylococcus aureus, varied in response to different EPS produced by different Bacillus spp. Proximate analysis, dinitrosalicylic acid method, pH meter measurement, total plate count by serial dilutions and spread plate technique, spectrophotometry in antioxidant activity assays and bioactive content assays, and agar disk diffusion were the principal involved methods. This work provides an alternative sustainable strategy of zero-waste management in the agricultural sector using microbial cell factories as a tool for valorizing agro-biowaste, such as riceberry broken rice, into a value-added bioactive EPS bioproduct that contributes to a bio-circular green economy in the country

Raw dataset for bacterial exopolysaccharide production using riceberry broken rice as precursors by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This raw dataset reflects an analysis of the antioxidant and antibacterial activity of exopolysaccharide (EPS) produced by seven Bacillus spp. isolated from Thai milk kefir [1] on a substrate of riceberry broken rice. Riceberry broken rice was analyzed for its moisture, ash, fat, protein, fiber, starch, sugar, hemicellulose, and cellulose plus lignin content when used as an EPS-producing substrate. After 3 days of fermentation, dataset on the total plate count, the reducing sugar content, the pH of the culture, and the fresh and dry weight of the EPS was recorded. In response to the EPS produced by various Bacillus spp., the total phenolic content, total flavonoid content, antimicrobial activity against Streptococcus agalactiae and Staphylococcus aureus, and hydroxyl radical scavenging activity were collected. Agar disk diffusion, spectrophotometry for antioxidant activity and bioactive content, proximate analysis, the dinitrosalicylic acid method, pH meter readings, the total plate count via serial dilutions, and the spread plate technique were the primary techniques used

Raw dataset for bacterial exopolysaccharide production using riceberry broken rice as precursors by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This raw dataset reflects an analysis of the antioxidant and antibacterial activity of exopolysaccharide (EPS) produced by seven Bacillus spp. isolated from Thai milk kefir [1] on a substrate of riceberry broken rice. Riceberry broken rice was analyzed for its moisture, ash, fat, protein, fiber, starch, sugar, hemicellulose, and cellulose plus lignin content when used as an EPS-producing substrate. After 3 days of fermentation, dataset on the total plate count, the reducing sugar content, the pH of the culture, and the fresh and dry weight of the EPS was recorded. In response to the EPS produced by various Bacillus spp., the total phenolic content, total flavonoid content, antimicrobial activity against Streptococcus agalactiae and Staphylococcus aureus, and hydroxyl radical scavenging activity were collected. Agar disk diffusion, spectrophotometry for antioxidant activity and bioactive content, proximate analysis, the dinitrosalicylic acid method, pH meter readings, the total plate count via serial dilutions, and the spread plate technique were the primary techniques used.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Raw dataset for bacterial exopolysaccharide production using riceberry broken rice as precursors by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This raw dataset reflects an analysis of the antioxidant and antibacterial activity of exopolysaccharide (EPS) produced by seven Bacillus spp. isolated from Thai milk kefir [1] on a substrate of riceberry broken rice. Riceberry broken rice was analyzed for its moisture, ash, fat, protein, fiber, starch, sugar, hemicellulose, and cellulose plus lignin content when used as an EPS-producing substrate. After 3 days of fermentation, dataset on the total plate count, the reducing sugar content, the pH of the culture, and the fresh and dry weight of the EPS was recorded. In response to the EPS produced by various Bacillus spp., the total phenolic content, total flavonoid content, antimicrobial activity against Streptococcus agalactiae and Staphylococcus aureus, and hydroxyl radical scavenging activity were collected. Agar disk diffusion, spectrophotometry for antioxidant activity and bioactive content, proximate analysis, the dinitrosalicylic acid method, pH meter readings, the total plate count via serial dilutions, and the spread plate technique were the primary techniques used.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Data on bacterial exopolysaccharide production using riceberry broken rice as precursors by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This data represented the evaluation of exopolysaccharide (EPS) production by Bacillus spp. isolated from Thai milk kefir [1] using riceberry broken rice as substrate and evaluating its antioxidant and antimicrobial activities. In Thailand, riceberry broken rice is an agricultural residue of several million tons per year from Thailand’s rice mill industry that is mostly not efficiently used or bio-transformed into more value-added bioproducts. Certain bacterial exopolysaccharides exhibit antioxidant and antimicrobial activities, which make them useful in the food, feed, pharmaceutical, and cosmetic industries. Chemical composition analysis of riceberry broken rice as an EPS substrate provided moisture, ash, fat, protein, fiber, starch, sugar, hemicellulose, and cellulose plus lignin content data. EPS production content in fresh and dry weight, pH of culture, reducing sugar content, and total plate count of bacteria were reported after 3-day fermentation. Antioxidant activities and bioactive content, including hydroxyl radical scavenging activity, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, ferric reducing antioxidant power, total phenolic content, total flavonoid content, and antimicrobial activity towards two bacterial pathogens of Nile tilapia, Streptococcus agalactiae, and Staphylococcus aureus, varied in response to different EPS produced by different Bacillus spp. Proximate analysis, dinitrosalicylic acid method, pH meter measurement, total plate count by serial dilutions and spread plate technique, spectrophotometry in antioxidant activity assays and bioactive content assays, and agar disk diffusion were the principal involved methods. This work provides an alternative sustainable strategy of zero-waste management in the agricultural sector using microbial cell factories as a tool for valorizing agro-biowaste, such as riceberry broken rice, into a value-added bioactive EPS bioproduct that contributes to a bio-circular green economy in the country.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Data on bacterial exopolysaccharide production using riceberry broken rice as precursors by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This data represented the evaluation of exopolysaccharide (EPS) production by Bacillus spp. isolated from Thai milk kefir [1] using riceberry broken rice as substrate and evaluating its antioxidant and antimicrobial activities. In Thailand, riceberry broken rice is an agricultural residue of several million tons per year from Thailand’s rice mill industry that is mostly not efficiently used or bio-transformed into more value-added bioproducts. Certain bacterial exopolysaccharides exhibit antioxidant and antimicrobial activities, which make them useful in the food, feed, pharmaceutical, and cosmetic industries. Chemical composition analysis of riceberry broken rice as an EPS substrate provided moisture, ash, fat, protein, fiber, starch, sugar, hemicellulose, and cellulose plus lignin content data. EPS production content in fresh and dry weight, pH of culture, reducing sugar content, and total plate count of bacteria were reported after 3-day fermentation. Antioxidant activities and bioactive content, including hydroxyl radical scavenging activity, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, ferric reducing antioxidant power, total phenolic content, total flavonoid content, and antimicrobial activity towards two bacterial pathogens of Nile tilapia, Streptococcus agalactiae, and Staphylococcus aureus, varied in response to different EPS produced by different Bacillus spp. Proximate analysis, dinitrosalicylic acid method, pH meter measurement, total plate count by serial dilutions and spread plate technique, spectrophotometry in antioxidant activity assays and bioactive content assays, and agar disk diffusion were the principal involved methods. This work provides an alternative sustainable strategy of zero-waste management in the agricultural sector using microbial cell factories as a tool for valorizing agro-biowaste, such as riceberry broken rice, into a value-added bioactive EPS bioproduct that contributes to a bio-circular green economy in the country.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Raw dataset for bacterial exopolysaccharide production using cassava pulp as substrate by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This raw dataset reflects an analysis of the antioxidant and antibacterial activity of exopolysaccharide (EPS) produced by eight Bacillus spp. isolated from Thai milk kefir [1] on a substrate of cassava pulp. Cassava pulp was analyzed for its moisture, ash, fat, protein, fiber, starch and sugar, neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL) when used as an EPS-producing substrate. After 3 days of fermentation, dataset on the total plate count, the reducing sugar content, the pH of the culture, and the fresh and dry weight of the EPS was recorded. In response to the EPS produced by several Bacillus spp., the total phenolic content, total flavonoid content, antimicrobial activity against Streptococcus agalactiae and Staphylococcus aureus, and antioxidant activities assessed by DPPH scavenging assay, FRAP assay and hydroxyl radical scavenging activity were collected. Agar disc diffusion, spectrophotometry for antioxidant activity and bioactive content, proximate analysis, the dinitrosalicylic acid method, pH meter readings, the total plate count via serial dilutions using the spread plate technique were the primary techniques used.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV