Data on bacterial exopolysaccharide production using riceberry broken rice as precursors by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

Abstract

This data represented the evaluation of exopolysaccharide (EPS) production by Bacillus spp. isolated from Thai milk kefir [1] using riceberry broken rice as substrate and evaluating its antioxidant and antimicrobial activities. In Thailand, riceberry broken rice is an agricultural residue of several million tons per year from Thailand’s rice mill industry that is mostly not efficiently used or bio-transformed into more value-added bioproducts. Certain bacterial exopolysaccharides exhibit antioxidant and antimicrobial activities, which make them useful in the food, feed, pharmaceutical, and cosmetic industries. Chemical composition analysis of riceberry broken rice as an EPS substrate provided moisture, ash, fat, protein, fiber, starch, sugar, hemicellulose, and cellulose plus lignin content data. EPS production content in fresh and dry weight, pH of culture, reducing sugar content, and total plate count of bacteria were reported after 3-day fermentation. Antioxidant activities and bioactive content, including hydroxyl radical scavenging activity, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, ferric reducing antioxidant power, total phenolic content, total flavonoid content, and antimicrobial activity towards two bacterial pathogens of Nile tilapia, Streptococcus agalactiae, and Staphylococcus aureus, varied in response to different EPS produced by different Bacillus spp. Proximate analysis, dinitrosalicylic acid method, pH meter measurement, total plate count by serial dilutions and spread plate technique, spectrophotometry in antioxidant activity assays and bioactive content assays, and agar disk diffusion were the principal involved methods. This work provides an alternative sustainable strategy of zero-waste management in the agricultural sector using microbial cell factories as a tool for valorizing agro-biowaste, such as riceberry broken rice, into a value-added bioactive EPS bioproduct that contributes to a bio-circular green economy in the country.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV