A CUSTOMARY ON SHOULDER PAIN POPULATION USING SIMPLE SHOULDER TEST

The purpose of the study is about to identify shoulder pain using a simple shoulder test among the population. BACKGROUND: Shoulder pain is the third most common musculoskeletal complaint encountered in clinical practice. Out of that, rotator cuff tendonitis is one of causes of shoulder pain. Rotator cuff tendonitis is an inflammation of the rotator cuff tendons without a rotator cuff tear. METHODOLOGY: This study is a prevalence study of pre and post test type that was conducted in 500 participants who have shoulder pain are selected and given simple shoulder test questionnaires. The participants with shoulder pain are between the age group of 18 and 60 years and both males and females were included in the study after getting their consent for participations. In these survey the person who is pregnant, who had history of surgery, presence of tumor, cardiac disease, person with cancer, mental disorder, recent fracture and dislocation of joints were excluded from the survey questionnaires. The questionnaires are based on the simple shoulder test, yes or no type with various clinical trials. RESULT : Of the 500 people who completed baseline questionnaires 51% were female and 49% were male. The mean age group is between 18 and 60 years. The result indicates that simple shoulder test questionnaires interpreted 50% of the population with RCT, 26%  with RA, and 24% with DJD. CONCLUSION: The present concludes that simple shoulder test questionnaires are an excellent tool to analyse shoulder pain. Out of 500 patients, 50% of shoulder pain is due to RCT, 26% of pain is due to RA, and 24% is due to DJD

Sub-clinical diabetic cardiomyopathy - assessment by systolic time intervals

Left ventricular performance in diabetics wit he at hypertension, ischemic Tie art disease, or clinical evidence of other heart diseases was assessed by systolic time intervals and echo-cardiography. The PEP/ LVET ratio was 3.48% higher in diabetics than in controls. There was good correlation of abnormality of PEP/LVET ratio with duration of diabetes. There was no correlation with age of patient or severity of diabetes. There was good correlation between abnormal PEP/LVET and incidence of retinopathy and nephropathy. This suggests the possibility of the presence of subclinical diabetic cardiomyopathy in these individuals

Oncogenic KRAS engages an RSK1/NF1 pathway to inhibit wild-type RAS signaling in pancreatic cancer.

Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy with limited treatment options. Although activating mutations of the KRAS GTPase are the predominant dependency present in >90% of PDAC patients, targeting KRAS mutants directly has been challenging in PDAC. Similarly, strategies targeting known KRAS downstream effectors have had limited clinical success due to feedback mechanisms, alternate pathways, and dose-limiting toxicities in normal tissues. Therefore, identifying additional functionally relevant KRAS interactions in PDAC may allow for a better understanding of feedback mechanisms and unveil potential therapeutic targets. Here, we used proximity labeling to identify protein interactors of active KRAS in PDAC cells. We expressed fusions of wild-type (WT) (BirA-KRAS4B), mutant (BirA-KRAS4BG12D), and nontransforming cytosolic double mutant (BirA-KRAS4BG12D/C185S) KRAS with the BirA biotin ligase in murine PDAC cells. Mass spectrometry analysis revealed that RSK1 selectively interacts with membrane-bound KRASG12D, and we demonstrate that this interaction requires NF1 and SPRED2. We find that membrane RSK1 mediates negative feedback on WT RAS signaling and impedes the proliferation of pancreatic cancer cells upon the ablation of mutant KRAS. Our findings link NF1 to the membrane-localized functions of RSK1 and highlight a role for WT RAS signaling in promoting adaptive resistance to mutant KRAS-specific inhibitors in PDAC

NIST interlaboratory study on glycosylation analysis of monoclonal antibodies : comparison of results from diverse analytical methods

Glycosylation is a topic of intense current interest in the development of biopharmaceuticals since it is related to drug safety and efficacy. This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material. Seventy‑six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions. The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation  analysis of mAbs. Therefore, participation was unrestricted, with laboratories choosing their own measurement techniques. Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification. Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48. In total, one hundred sixteen glycan compositions were reported, of which 57 compositions could be assigned consensus abundance values. These consensus medians provide community-derived values for NISTmAb PS. Agreement with the consensus medians did not depend on the specific method or laboratory type.. The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods

Diabetic cardiomyopathy

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Numerical Modeling and Symmetry Analysis of a Pine Wilt Disease Model Using the Mittag–Leffler Kernel

The existence of man is dependent on nature, and this existence can be disturbed by either man-made devastations or by natural disasters. As a universal phenomenon in nature, symmetry has attracted the attention of scholars. The study of symmetry provides insights into physics, chemistry, biology, and mathematics. One of the most important characteristics in the expressive assessment and development of computational design techniques is symmetry. Yet, mathematical models are an important method of studying real-world systems. The symmetry reflected by such a mathematical model reveals the inherent symmetry of real-world systems. This study focuses on the contagious model of pine wilt disease and symmetry, employing the q-HATM (q-Homotopy Analysis Transform Method) to the leading fractional operator Atangana–Baleanu (AB) to arrive at better understanding. The outgrowths are exhibited in the forms of figures and tables. Finally, the paper helps to analyze the practical theory, assisting the prediction of its manner that corresponds to the guidelines when contemplating the replica