Neurotic states at students with a hypodynamia

Mental health of the person depends on his physical state. Need of sports exists throughout human life. Restriction of motor activity of a body, lack of physical exercises negatively influences both physical health, and the mental healthПсихическое здоровье человека зависит от его физического состояния. Необходимость занятия спортом существует на протяжении жизни человека. Ограничение моторной деятельности тела, недостаток физических упражнений отрицательно влияет как на физическое здоровье, так и на психическо

A high sensitivity ZENK monoclonal antibody to map neuronal activity in Aves

The transcription factor ZENK is an immediate early gene that has been employed as a surrogate marker to map neuronal activity in the brain. It has been used in a wide variety of species, however, commercially available antibodies have limited immunoreactivity in birds. To address this issue we generated a new mouse monoclonal antibody, 7B7-A3, raised against ZENK from the rock pigeon (Columba livia). We show that 7B7-A3 labels clZENK in both immunoblots and histological stainings with high sensitivity and selectivity for its target. Using a sound stimulation paradigm we demonstrate that 7B7-A3 can detect activity-dependent ZENK expression at key stations of the central auditory pathway of the pigeon. Finally, we compare staining efficiency across three avian species and confirm that 7B7-A3 is compatible with immunohistochemical detection of ZENK in the rock pigeon, zebra finch, and domestic chicken. Taken together, 7B7-A3 represents a useful tool for the avian neuroscience community to map functional activity in the brain

Nanodomain structure evolution investigation during polarization reversal of lithium niobate monocrystals with modified surface layers by proton exchange with different thicknesses

Целью работы является исследование эволюции формы микродоменов и формирования нанодоменных структур в ниобате лития с поверхностным слоем, модифицированным методом протонного обмена различной толщины. Работа направлена на решение фундаментальных и прикладных проблем физики сегнетоэлектриков. Целью работы является экспериментальное исследование формирования и эволюции микро- и нанодоменных структур в неравновесных условиях, обусловленных неэффективным экранированием деполяризующих полей, реализуемых при переключении поляризации в монокристаллах ниобата лития с поверхностным слоем, модифицированным методом протонного обмена. Исследование влияния поверхностных диэлектрических слоев на кинетику переключения поляризации имеет важное фундаментальное и прикладное значение. Изучение кинетики доменов в присутствии диэлектрических слоев важно для выяснения роли естественного поверхностного диэлектрического слоя в наблюдаемых закономерностях динамики доменов. С практической стороны, использование слоев с контролируемыми свойствами открывает дополнительные возможности для управления кинетикой и геометрией доменной структуры и разработки новых методов построения различных акустических, нелинейно-оптических и электро-оптических устройств с помощью доменной инженерии. В качестве объекта исследования в данной работе используется ниобат лития LiNbO3, различные модификации которого на сегодняшний день являются наиболее востребованными материалами для построения большого класса устройств нелинейной оптики, в том числе с использованием подходов доменной инженерии. В работе будет исследован ниобат лития конгруэнтного состава (CLN). В качестве методики модификации поверхностного слоя СLN используется классическая техника создания волноводов – протонный обмен (PE), который позволяет создавать на поверхности диэлектрические слои с хорошо контролируемыми параметрами (глубиной, формой профиля и поверхностной проводимостью). Сочетание важного для применений материала и разработанной методики модификации поверхности обусловливает актуальность проводимых исследований. Основные результаты, полученные в хоте выполнения работы: • Установлено, что увеличение порогового поля пропорционально толщине модифицированного слоя. • Показано, что при переключении поляризации в растущем поле независимо от толщины PE-слоя кинетика доменной структуры начинается с появления и роста шестиугольных доменов; • Установлено, что в образцах с толщиной модифицированного слоя 1,7 и 3,4 мкм наблюдается два типа эволюции доменных структур: 1) рост «широкой доменной границы» (wide domain boundary - WDB) в слабом и среднем поле и 2) формирование микро- и нанодоменных ансамблей в сильном поле. • Обнаружено, что в образцах с толщиной модифицированного слоя 4,6 мкм реализуется дискретное переключение, и формируются паутинообразные доменные структуры, состоящие из изолированных доменов. • Показано, что полевые зависимости скорости распространения фронта нанодоменов могут быть аппроксимированы активационной зависимостью с полем активации Ea = 3,3 ± 0,1 кВ/мм. Место выполнения работы. Работа выполнена в лаборатории сегнетоэлектриков НИИ физики и прикладной математики Института естественных наук Уральского федерального университета имени первого Президента России Б.Н.Ельцина на оборудовании Уральского центра коллективного пользования «Современные нанотехнологии» ИЕН УрФУ.Программа развития УрФУ на 2013 год (п.2.1.2.1

On a snow cover composition in the vicinity of the Verkhnekamsky Salt Deposit

The snow cover chemical composition was investigated on the territory of the Verkhnekamskiy potash-magnesium salt deposit in the zone of influence of atmospheric emissions of Berezniki Potash Mining Complex (Perm Region). The object of researches was the snow in this area. Features of specific pollutants contained in the emissions of these mine groups and their relationship with the air intake and following atmospheric deposition were examined. With regard for specific character of pollutants in the emissions, studies of melt water included determination of the following chemical components – SO42-, Cl-, NO3-, NO2-, НCO3-, Ca2+, Mg2+, Na+, K+, of the pH value as well as suspended solid substances, and total mineralization. An analysis of snow composition was performed on two sites from which one was the surrounding background territory, while another area was the urban territory of city Berezniki, i.e. area of operating mine group. To assess the results the indicator of specific stock macro component was used. Increased specific stock relative to the background snowpack is clearly indicative of components directly related to the extraction and processing of salts Cl-, Na+, K+. The revealed anomalies have a local character and, as a rule, do not extend beyond boundaries of sanitary protection zones of the enterprises. Maps of distribution of values of the specific reserves for the analyzed list of components in the melt water were constructed. Our results obtained for the Verkhnekamskiy Deposit area were compared with similar published data on the territories of cities in the zone influenced by motor roads, reserves, and coastal zones of the Russian Arctic seas

The expression, localisation and interactome of pigeon CRY2

Cryptochromes (CRY) are highly conserved signalling molecules that regulate circadian rhythms and are candidate radical pair based magnetoreceptors. Birds have at least four cryptochromes (CRY1a, CRY1b, CRY2, and CRY4), but few studies have interrogated their function. Here we investigate the expression, localisation and interactome of clCRY2 in the pigeon retina. We report that clCRY2 has two distinct transcript variants, clCRY2a, and a previously unreported splice isoform, clCRY2b which is larger in size. We show that clCRY2a mRNA is expressed in all retinal layers and clCRY2b is enriched in the inner and outer nuclear layer. To define the localisation and interaction network of clCRY2 we generated and validated a monoclonal antibody that detects both clCRY2 isoforms. Immunohistochemical studies revealed that clCRY2a/b is present in all retinal layers and is enriched in the outer limiting membrane and outer plexiform layer. Proteomic analysis showed clCRY2a/b interacts with typical circadian molecules (PER2, CLOCK, ARTNL), cell junction proteins (CTNNA1, CTNNA2) and components associated with the microtubule motor dynein (DYNC1LI2, DCTN1, DCTN2, DCTN3) within the retina. Collectively these data show that clCRY2 is a component of the avian circadian clock and unexpectedly associates with the microtubule cytoskeleton

Immunotropic effects of vitamin D3 in original rectal suppositories in experimental ulcerative colitis

Increased incidence of ulcerative colitis (UC) is a prerequisite for searching new therapeutic approaches, primarily with an opportunity of site-directed impact on the colon lesion. UC pathogenesis is associated with dysregulated immune response, and limited effectiveness of basic therapy for the disorder. Vitamin D3 exhibits antioxidant, anti-inflammatory, immunomodulatory and other properties, it has been shown to be effective in some autoimmune diseases, thus prompting us to study its effect on immune status in UC. We aimed for studying the effect of vitamin D3, as a component of original rectal suppositories, upon clinical course and indexes of immune status in experimental UC. UC in rats was modeled with 3% oxazolone solution. The vitamin D3-containing suppositories (1500 IU) weighing 300 mg were administered per rectum every 12 hours for 6 days. On days 2, 4 and 6 of UC, the clinical features were assessed as well as blood leukocyte counts, numbers of CD3+, CD45RA+; absorbing and NBT-reducing abilities of blood neutrophils were determined; IgM, IgG, IL-6 and IL-8 concentrations in serum were also studied.The DAI index increased in non-treated UC, along with raised neutrophil numbers in blood, their absorption and NBT-reducing activity was also increased, the total number of lymphocytes, including CD3+, CD45RA+ became higher, serum concentrations of IgM, IgG, IL-6, IL-8 increased. Local use of vitamin D3 in UC reduces DAI parameters, causes decrease in blood neutrophil counts, reducing and partially restoring absorptive and NBT-reducing abilities of neutrophils, decline of total lymphocyte counts in blood, partially restoring the CD3+ and CD45RA+ numbers, causing decline and partial restoration of serum IgM, IgG, IL-6, IL-8 concentrations. An association between clinical signs and indexes of immune status in UC was established under the conditions of vitamin D3 use. Conclusions: The protective effect of vitamin D3 in UC can be mediated by its antioxidant effect, changes in production of immunoregulatory cytokines, modulation of Th1-, Th2-, Th17-dependent reactions and Treg activity, being a pre-requisite for further studies to clarify the mechanism of vitamin D3 immunotropic action in UC,with an opportunity of using it in clinical practice

Экогеохимическая и биоиндикационная оценка загрязнения малых рек города Березники

This paper presents an assessment of the state of the rivers Tolych, Zyryanka and Bygel, flowing within the area with a high technogenic impact (Berezniki, Perm krai). Water quality was evaluated using the integrated geochemical parameters. The characteristics of the macrozoobenthos have been studied as a bioindicator of the ecological state of the reservoir. The formation of the trace element composition and, consequently, water quality is associated with both natural and anthropogenic factors. The obtained metal pollution index (MI) demonstrates the unsuitability of water from rivers Tolych, Zyryanka, and Bygel for domestic purposes.В данном исследовании представлены результаты оценки состояния рек Толыч, Зырянка и Быгель, протекающих в зоне с высокой техногенной нагрузкой (г. Березники, Пермский край). Проведена оценка качества воды с использованием интегральных геохимических показателей. Характеристики макрозообентоса изучены в качестве биоиндикатора экологического состояния водотоков. Формирование микроэлементного состава и, как следствие, качества вод связано как с естественными, так и с антропогенными факторами. В данном исследовании показана важность выбора подхода к оценке качества вод. Результаты расчета индекса нагрузки металлами (MI) демонстрируют непригодность водных биологических ресурсов рек Толыч, Зырянка и Быгель для использования в питании человека

From colloidal CdSe quantum dots to microscale optically anisotropic supercrystals through bottom-up self-assembly

This is the author accepted manuscript. The final version is available on open access from Royal Society of Chemistry via the DOI in this recordThe development of fabrication techniques for novel nanostructured materials is one of the key tasks of modern materials science. One pathway to successfully complete this task is the bottom-up assembly of colloidal nanoparticles into ordered superstructures, possessing both the properties of individual nanoparticles and further novel properties resulting from their interactions. However, nanoparticle self-assembly depends on a variety of parameters, which makes the precise control of this process a complicated problem. Here, the time course of quantum dot (QD) self-assembly into ordered superstructures has been analyzed, along with the evolution of their morphological and optical properties. QD self-assembly occurs through two distinct stages (homo- and hetero-geneous), leading to the formation of supercrystals with a layered morphology. Analysis of the optical properties throughout the superstructures’ growth has shown that the absorption and photoluminescence (PL) bands are blue shifted, retaining almost the same PL lifetimes as in the initial QD solution. The supercrystals formed possess a further unique optical property caused by their layered morphology; namely, a four-fold symmetry characterized by strong birefringence. Such supercrystals may be used for the fabrication of microscale optical paths with high extinction coefficients and specific polarization properties for novel optoelectronic devices.This study was supported by the Ministry of Education and Science of the Russian Federation through the grant No. 14.584.21.0032 (ID RFMEFI58417X0032), the Engineering and Physical Sciences Research Council (EPSRC) of the United Kingdom via the EPSRC Centre for Doctoral Training in Electromagnetic Metamaterials (Grant No. EP/L015331/1) and via EP/N035569/1, and the Royal Society via International Exchange Grant No. 2016/R1

Psychological features of the people subject to ASMR-to effect

In article, we considered a phenomenon of ASMR and its interrelation with the leading representative system, suggestibility degree, field dependence and field independence and touch sensitivity.В статье рассмотрен феномен АСМР и его взаимосвязь с ведущей репрезентативной системой, степенью внушаемости, полезависимостью-поленезависимостью и сенсорной чувствительностью