Rodzice–szkoła i szkoła–rodzice: wzajemne oczekiwania

Artykuł z numeru 1/2012 internetowego czasopisma edukacyjnego ORE "Trendy

Road to clinical implementation of CAR-T technology in Poznań

The objective of this paper is to present the process of the national and international accreditation leading to the establishment of the first certified chimeric antigen receptor T (CAR-T) Cell Unit in Poland on the basis of the Department of Hematology and Bone Marrow Transplantation in Poznan University of Medical Sciences and first successful CAR-T therapy in Poland. During 12 months from the initial decision to establish the CAR-T Cell Unit to the application of CAR-T cell treatment in the first patient, the center had to undergo the multidisciplinary external and internal training, as well as the adaptation of multiple procedures within the Transplant Unit and Stem Cell Bank. In order to get accreditation for the implementation of CAR-T cell therapy, an initial training of the team involved in handling cellular products and patient care was organized and updated as a continuous process. The Department fulfilled the site-selection international criteria. The first patient diagnosed for refractory/relapsed DLBCL was qualified, and finally CAR-T cells were administered with successful clinical outcome

Experimental tests of fluid exchange process improvement in a new design of hydraulic cylinder with a supply system

The article presents the results of experimental tests of the liquid exchange level in a new design of a hydraulic cylinder in comparison with the classic one. Comparative results are presented, as well as the influence of the stroke range on the degree of liquid exchange. The results allow the empirical determination of the degree of real improvement of the process of liquid exchange in the cylinder during operation, and thus allow the initial determination of the reliability level increase in hydraulic cylinders. It should be emphasized that the presented results are a part of a research and implementation project carried out in cooperation between a university and an industrial partner. In addition, the presented test method of hydraulic cylinders is, in the opinion of the authors, an interesting proposition, which can be used by manufacturers of this type of components in the world

Retrospective analysis of indications and complications related to implantation of permanent pacemaker: 25 years of experience in 31 dogs

Introduction: Pacemaker implantation is the only effective symptomatic treatment for life-threatening bradyarrhythmias. Major complications observed after implantation of cardiac pacemakers include lead dislocation, loss of pulse generator function, and inadequate stimulation. The aim of this retrospective single-centre study was to analyse the indications for pacemaker implantation and the incidence and types of complications associated with this procedure in dogs treated for symptomatic bradyarrhythmia

Expression and Complex Formation of MMP9, MMP2, NGAL, and TIMP1 in Porcine Myocardium but Not in Skeletal Muscles in Male Pigs with Tachycardia-Induced Systolic Heart Failure

Matrix metalloproteinases (MMPs) are involved in the remodeling of extracellular matrix in various tissues. Their functioning could be related to the formation of complexes, containing MMP9, MMP2, tissue inhibitor of metalloproteinases type 1 (TIMP1), and neutrophil gelatinase-associated lipocalin (NGAL). Such complexes have not been investigated in either myocardial or skeletal muscles. We examined 20 male pigs with heart failure (HF), and 5 sham-operated animals. There were no differences in the mRNA expression of MMP9, MMP2, TIMP1, and NGAL between diseased and healthy animals, in either left ventricle (LV) myocardium or skeletal muscles. In LV from both diseased and healthy animals, in nonreducing and nondenaturing conditions, we demonstrated the presence of high molecular weight (HMW) complexes (130, 170, and 220 kDa) containing MMP9, TIMP1, and NGAL (also MMP2 in 220 kDa complex) without proteolytic activity, and a proteolytically active 115 kDa MMP9 form together with 72 and 68 kDa bands (proMMP2 and MMP2). Proteolytically active bands were also spontaneously released from HMW complexes. In skeletal muscles from both diseased and healthy animals, in nonreducing and nondenaturing conditions, we found no HMW complexes, and proteolytic activity was associated with the presence of 72 and 68 kDa bands (proMMP2 and MMP2)

Additional file 1: Figure S1. of Sex differences in porcine left ventricular myocardial remodeling due to right ventricular pacing

Indices of LV function at different time points for sham-operated pigs and RV-paced pigs. A and B show changes over time in LVPWd (LVPWd—end-diastolic thickness of left ventricle posterior wall); C and D show LVPWs—end-systolic thickness of left ventricle posterior wall, E and F show SV (stroke volume) in right ventricular (RV) paced and sham-operated pigs; G and H show LA/Ao (left atrial/aorta ratio) separately in male and female animals (black squares—RV paced female pigs, black circles—RV paced male pigs, gray squares—female controls, gray circles—male controls). Values are presented as means ± SEM. Data (separately for control and TIC animals) were tested using one-way ANOVA. Figure S2. Relationship between BNP mRNA expression and echocardiographic parameters. Elevated BNP mRNA was significantly correlated with increased LVEDV in males (A) as well as with a decrease in LVEF in males (B) and females (C). Regression lines have been fit to all data points. Figure S3. Relationship between GATA4 mRNA expression and TGFβ1 and BNP expression level as well as LVEF. Elevated GATA4 mRNA was significantly correlated with increased BNP mRNA level in males (A) as well as in females (B). Only in males elevated GATA4 mRNA was also significantly correlated with TGFβ1 BNP mRNA level (C) and with a decrease in LVEF (D). Regression lines have been fit to all data points. Figure S4. Relationship between NGAL mRNA expression and LVEDV. Elevated NGAL mRNA was significantly correlated with increased LVEDV in males. Regression lines have been fit to all data points. Figure S5. Relationship between collagen mRNA level and TGFβ1 expression. Elevated Col1A1 (A), Col1A2 (B), and Col3A1 (C) mRNAs were significantly correlated with an increase in TGFβ1 mRNA level in males. In females increased expression of Col1A1 (D) and Col3A1 (E) was related to TGFβ1 mRNA level. Regression lines have been fit to all data points. Figure S6. Relationship between ECM turnover markers and echocardiographic parameters. (A) Reduced total gelatinolytic activity was significantly correlated with an increase in BNP mRNA level in males. (B) Decreasing water soluble collagen was related to an decrease in NGAL mRNA. (C) In male subjects from the mild HF group, the water soluble collagen correlated with the relative increase in LVEDV. Regression lines have been fit to all data points. Figure S7. Relationship between cardiomyocyte diameter and echocardiographic parameters. In females cardiomyocyte cross-sectional diameter correlated with LVEDV. Regression lines have been fit to all data points. Table S1. Initial values of echocardiography parameters in sham-operated male pigs (controls) and right ventricle paced pigs with induced heart failure. Table S2. Echocardiography parameters reflecting the structure and functioning of left ventricle in sham-operated male pigs (controls) and right ventricle paced pigs with induced heart failure. Table S3. Sex-related differences in selected echocardiography parameters assessed in left ventricular myocardium in male pigs with and without tachycardia-induced cardiomyopathy (the results of the two-way ANOVA). Table S4. Relationships between selected echocardiographic parameters and HF insufficiency in male and female pigs with and without tachycardia-induced cardiomyopathy (the results of Spearman’s rank correlatory coefficients)