426 research outputs found
Detection of the 5p-4f orbital crossing and its optical clock transition in Pr9+
Recent theoretical works have proposed atomic clocks based on narrow optical transitions in highly charged ions. The most interesting candidates for searches of new physics are those which occur at rare orbital crossings where the shell structure of the periodic table is reordered. There are only three such crossings expected to be accessible in highly charged ions, and hitherto none have been observed as both experiment and theory have proven difficult. In this work we observe an orbital crossing in highly charged ions for the first time, in a system chosen to be tractable from both sides: Pr9+. We present electron beam ion trap measurements of its spectra, including the inter-configuration lines that reveal the sought-after crossing. The proposed nHz-wide clock line, found to be at 452.334(1) nm, proceeds through hyperfine admixture of its upper state with an E2-decaying level. With state-of-the-art calculations we show that it has a very high sensitivity to new physics and extremely low sensitivity to external perturbations, making it a unique candidate for proposed precision studies
EUV spectroscopy of Sn5+-Sn(10+)ions in an electron beam ion trap and laser-produced plasmas
Emission spectra from multiply-charged Sn5+ –Sn10+ions are recorded from an electron beam ion trap (EBIT) and from laser-produced plasma (LPP) in the extreme ultraviolet range relevant for nanolithographic applications. Features in the wavelength regime between 12.6 and 20.8 nm are studied. Using the Cowan code, emission line features of the charge-state-resolved Sn ion spectra obtained from the EBIT are identified. Emission features from tin LPP either from a liquid micro-droplet or planar solid target are subsequently identified and assigned to specific charge states using the EBIT data. For the planar solid tin target, the 4d–5p transitions of Sn8+ –Sn10+ions are shown to dominate the long-wavelength part of the measured spectrum and transitions of type 4d–4f + 4p–4d are visible in absorption. For the droplet target case, a clear increase in the charge state distribution with increasing laser intensity is observed. This qualitatively demonstrates the potential of using long-wavelength out-of-band emission features to probe the charge states contributing to the strong unresolved transition array at 13.5 nm relevant for nanolithography
Evaluation of diet pattern and weight gain in postmenopausal women enrolled in the Women’s Health Initiative Observational Study
Abstract It is unclear which of four popular contemporary diet patterns is best for weight maintenance among postmenopausal women. Four dietary patterns were characterised among postmenopausal women aged 49–81 years (mean 63·6 ( sd 7·4) years) from the Women’s Health Initiative Observational Study: (1) a low-fat diet; (2) a reduced-carbohydrate diet; (3) a Mediterranean-style (Med) diet; and (4) a diet consistent with the US Department of Agriculture’s Dietary Guidelines for Americans (DGA). Discrete-time hazards models were used to compare the risk of weight gain (≥10 %) among high adherers of each diet pattern. In adjusted models, the reduced-carbohydrate diet was inversely related to weight gain (OR 0·71; 95 % CI 0·66, 0·76), whereas the low-fat (OR 1·43; 95 % CI 1·33, 1·54) and DGA (OR 1·24; 95 % CI 1·15, 1·33) diets were associated with increased risk of weight gain. By baseline weight status, the reduced-carbohydrate diet was inversely related to weight gain among women who were normal weight (OR 0·72; 95 % CI 0·63, 0·81), overweight (OR 0·67; 95 % CI 0·59, 0·76) or obese class I (OR 0·63; 95 % CI 0·53, 0·76) at baseline. The low-fat diet was associated with increased risk of weight gain in women who were normal weight (OR 1·28; 95 % CI 1·13, 1·46), overweight (OR 1·60; 95 % CI 1·40, 1·83), obese class I (OR 1·73; 95 % CI 1·43, 2·09) or obese class II (OR 1·44; 95 % CI 1·08, 1·92) at baseline. These findings suggest that a low-fat diet may promote weight gain, whereas a reduced-carbohydrate diet may decrease risk of postmenopausal weight gain
QED Effective Action at Finite Temperature: Two-Loop Dominance
We calculate the two-loop effective action of QED for arbitrary constant
electromagnetic fields at finite temperature T in the limit of T much smaller
than the electron mass. It is shown that in this regime the two-loop
contribution always exceeds the influence of the one-loop part due to the
thermal excitation of the internal photon. As an application, we study light
propagation and photon splitting in the presence of a magnetic background field
at low temperature. We furthermore discover a thermally induced contribution to
pair production in electric fields.Comment: 34 pages, 4 figures, LaTe
Stringent test of QED with hydrogenlike tin
Inner-shell electrons naturally sense the electric field close to the
nucleus, which can reach extreme values beyond
for the innermost electrons. Especially in few-electron highly charged ions,
the interaction with the electromagnetic fields can be accurately calculated
within quantum electrodynamics (QED), rendering these ions good candidates to
test the validity of QED in strong fields. Consequently, their Lamb shifts were
intensively studied in the last decades. Another approach is the measurement of
factors in highly charged ions. However, so far, either experimental
accuracy or small field strength in low- ions limited the stringency of
these QED tests. Here, we report on our high-precision, high-field test of QED
in hydrogenlike Sn. The highly charged ions were produced with
the Heidelberg-EBIT (electron beam ion trap) and injected into the ALPHATRAP
Penning-trap setup, where the bound-electron factor was measured with a
precision of 0.5 parts-per-billion. For comparison, we present state-of-the-art
theory calculations, which together test the underlying QED to about
, yielding a stringent test in the strong-field regime. With this
measurement, we challenge the best tests via the Lamb shift and, with
anticipated advances in the -factor theory, surpass them by more than an
order of magnitude
MAGE I Transcription Factors Regulate KAP1 and KRAB Domain Zinc Finger Transcription Factor Mediated Gene Repression
Class I MAGE proteins (MAGE I) are normally expressed only in developing germ cells but are aberrantly expressed in many cancers. They have been shown to promote tumor survival, aggressive growth, and chemoresistance but the underlying mechanisms and MAGE I functions have not been fully elucidated. KRAB domain zinc finger transcription factors (KZNFs) are the largest group of vertebrate transcription factors and regulate neoplastic transformation, tumor suppression, cellular proliferation, and apoptosis. KZNFs bind the KAP1 protein and direct KAP1 to specific DNA sequences where it suppresses gene expression by inducing localized heterochromatin characterized by histone 3 lysine 9 trimethylation (H3me3K9). Discovery that MAGE I proteins also bind to KAP1 prompted us to investigate whether MAGE I can affect KZNF and KAP1 mediated gene regulation. We found that expression of MAGE I proteins, MAGE-A3 or MAGE-C2, relieved repression of a reporter gene by ZNF382, a KZNF with tumor suppressor activity. ChIP of MAGE I (-) HEK293T cells showed KAP1 and H3me3K9 are normally bound to the ID1 gene, a target of ZNF382, but that binding is greatly reduced in the presence of MAGE I proteins. MAGE I expression relieved KAP1 mediated ID1 repression, causing increased expression of ID1 mRNA and ID1 chromatin relaxation characterized by loss of H3me3K9. MAGE I binding to KAP1 also induced ZNF382 poly-ubiquitination and degradation, consistent with loss of ZNF382 leading to decreased KAP1 binding to ID1. In contrast, MAGE I expression caused increased KAP1 binding to Ki67, another KAP1 target gene, with increased H3me3K9 and decreased Ki67 mRNA expression. Since KZNFs are required to direct KAP1 to specific genes, these results show that MAGE I proteins can differentially regulate members of the KZNF family and KAP1 mediated gene repression
X-ray resonant photoexcitation: line widths and energies of K{\alpha} transitions in highly charged Fe ions
Photoabsorption by and fluorescence of the K{\alpha} transitions in highly
charged iron ions are essential mechanisms for X-ray radiation transfer in
astrophysical environments. We study photoabsorption due to the main K{\alpha}
transitions in highly charged iron ions from heliumlike to fluorinelike (Fe
24+...17+) using monochromatic X-rays around 6.6 keV at the PETRA III
synchrotron photon source. Natural linewidths were determined with hitherto
unattained accuracy. The observed transitions are of particular interest for
the understanding of photoexcited plasmas found in X-ray binaries and active
galactic nuclei.Comment: Revised versio
ZZW-115-dependent inhibition of NUPR1 nuclear translocation sensitizes cancer cells to genotoxic agents
Establishing the interactome of the cancer-associated stress protein Nuclear Protein 1 (NUPR1), we found that it binds to several hundreds of proteins, including proteins involved in nuclear translocation, DNA repair, and key factors of the SUMO pathway. We demonstrated that the NUPR1 inhibitor ZZW-115, an organic synthetic molecule, competes with importins for the binding to the NLS region of NUPR1, thereby inhibiting its nuclear translocation. We hypothesized, and then proved, that inhibition of NUPR1 by ZZW-115 sensitizes cancer cells to DNA damage induced by several genotoxic agents. Strikingly, we found that treatment with ZZW-115 reduced SUMOylation of several proteins involved in DNA damage response (DDR). We further report that the presence of recombinant NUPR1 improved the SUMOylation in a cell-free system, indicating that NUPR1 directly stimulates the SUMOylation machinery. We propose that ZZW-115 sensitizes cancer cells to genotoxic agents by inhibiting the nuclear translocation of NUPR1 and thereby decreasing the SUMOylation-dependent functions of key proteins involved in the DDR
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