The helicase HAGE prevents interferon-a-induced PML expression in ABCB5+ malignant melanoma-initiating cells by promoting the expression of SOCS1

The tumour suppressor PML (promyelocytic leukaemia protein) regulates several cellular pathways involving cell growth, apoptosis, differentiation and senescence. PML also has an important role in the regulation of stem cell proliferation and differentiation. Here, we show the involvement of the helicase HAGE in the transcriptional repression of PML expression in ABCB5 + malignant melanoma-initiating cells (ABCB5 + MMICs), a population of cancer stem cells which are responsible for melanoma growth, progression and resistance to drug-based therapy. HAGE prevents PML gene expression by inhibiting the activation of the JAK-STAT (janus kinase-signal transducers and activators of transcription) pathway in a mechanism which implicates the suppressor of cytokine signalling 1 (SOCS1). Knockdown of HAGE led to a significant decrease in SOCS1 protein expression, activation of the JAK-STAT signalling cascade and a consequent increase of PML expression. To confirm that the reduction in SOCS1 expression was dependent on the HAGE helicase activity, we showed that SOCS1, effectively silenced by small interfering RNA, could be rescued by re-introduction of HAGE into cells lacking HAGE. Furthermore, we provide a mechanism by which HAGE promotes SOCS1 mRNA unwinding and protein expression in vitro

Migrant healthcare workers during COVID-19: bringing an intersectional health system-related approach into pandemic protection. A German case study

IntroductionMigrant healthcare workers played an important role during the COVID-19 pandemic, but data are lacking especially for high-resourced European healthcare systems. This study aims to research migrant healthcare workers through an intersectional health system-related approach, using Germany as a case study.MethodsAn intersectional research framework was created and a rapid scoping study performed. Secondary analysis of selected items taken from two COVID-19 surveys was undertaken to compare perceptions of national and foreign-born healthcare workers, using descriptive statistics.ResultsAvailable research is focused on worst-case pandemic scenarios of Brazil and the United Kingdom, highlighting racialised discrimination and higher risks of migrant healthcare workers. The German data did not reveal significant differences between national-born and foreign-born healthcare workers for items related to health status including SARS-CoV-2 infection and vaccination, and perception of infection risk, protective workplace measures, and government measures, but items related to social participation and work conditions with higher infection risk indicate a higher burden of migrant healthcare workers.ConclusionsCOVID-19 pandemic policy must include migrant healthcare workers, but simply adding the migration status is not enough. We introduce an intersectional health systems-related approach to understand how pandemic policies create social inequalities and how the protection of migrant healthcare workers may be improved

DMTs and Covid-19 severity in MS: a pooled analysis from Italy and France

We evaluated the effect of DMTs on Covid-19 severity in patients with MS, with a pooled-analysis of two large cohorts from Italy and France. The association of baseline characteristics and DMTs with Covid-19 severity was assessed by multivariate ordinal-logistic models and pooled by a fixed-effect meta-analysis. 1066 patients with MS from Italy and 721 from France were included. In the multivariate model, anti-CD20 therapies were significantly associated (OR = 2.05, 95%CI = 1.39–3.02, p < 0.001) with Covid-19 severity, whereas interferon indicated a decreased risk (OR = 0.42, 95%CI = 0.18–0.99, p = 0.047). This pooled-analysis confirms an increased risk of severe Covid-19 in patients on anti-CD20 therapies and supports the protective role of interferon

Acute myocardial infarction following thalidomide treatment for AIDS-related ulcers.

SCOPUS: le.jinfo:eu-repo/semantics/publishe

Syntheses and characterization of new organically grafted silica foams

In this study, new hybrid Organo-Si(HIPE) macrocellular foams have been synthesized via sol–gel process, direct emulsions and lyotropic mesophases as templating agents followed by a final grafting process of silane organically modified. A variety of sililated groups, including methyl, benzyl, amines, mercapto, pyrrol and dinitro have been successfully grafted. The effects of the various organosilanes on the physical chemical properties of the resulting materials have been thoroughly characterized using several analytical techniques (SEM, TEM, SAXS, mercury porosimetry, nitrogen adsorption isotherms, FTIR, 29Si MAS NMR). If the resulting grafted compounds, labeled gOrgano Si(HIPE), exhibit the same macroscopic texture than the starting silica open-cell matrices, we found a reduction of the mesoporosity depending upon the size of the organic moiety in use and the associated enhance molecular hindrance. Final porous materials functionalized at the molecular level are potential outstanding candidates for applications such as heterogeneous catalyst, scavenger and sensor

Herniation of an abdominal antireflux fundoplication into the chest: what does it mean?

OBJECTIVES: The specific contribution of the herniation of an abdominal antireflux fundoplication into the chest to symptomatic and therefore surgical failure remains unclear. METHODS: The study was conducted in 189 consecutive fundoplication patients, categorized as patients reoperated on for chest herniation of either an abdominal 360° (Group 1; n = 95) or a partial (Group 2; n = 10) fundoplication, and patients having undergone an intrathoracic 360° fundoplication for short oesophagus (Group 3; n = 84; reference group). There were four subgroups in Group 1: 1A: wrap still complete and perioesophageal; 1B: wrap still complete but perigastric; 1C: wrap still perioesophageal but partially disrupted and 1D: wrap perigastric and partially disrupted. RESULTS: The prevalence of defective symptoms (heartburn and regurgitation) was significantly lower (P < 0.0001) in Group 3 (0.0%) and Subgroup 1A (3.7%) than in Subgroups 1B (84.4%), 1C (86.7%) and 1D (100%) and Group 2 (100%). The prevalence of obstructive symptoms (dysphagia, chest pain, necrosis and perforation) was significantly higher (P < 0.0001) in Subgroup 1A (100%) than in Subgroups 1B (57.8%), 1C (60.0%) and 1D (25.0%). The prevalence of a short oesophagus, an abdominal wall hernia repair and high abdominal pressure episodes in reoperated patients were 13.7, 36.2 and 67.2%, respectively. CONCLUSIONS: Unlike perigastric or partial fundoplication, a 360° perioesophageal abdominal fundoplication, when herniated into the chest, is still effective against reflux. Obstructive symptoms are due to either diaphragmatic strangulation or perigastric migration of the wrap (slipknot effect). Short oesophagus, weakness of the abdominal wall and high abdominal pressure episodes favour the herniation process

A cobalt oxide–polypyrrole nanocomposite as an efficient and stable electrode material for electrocatalytic water oxidation

International audienceDeveloping electrolyzers operating under neutral or near-neutral conditions with catalysts based only on earth-abundant metals is highly desirable with a view to reduce the cost of hydrogen production from water splitting reaction and avoid the environmental issues related to corrosion usually encountered with alkaline electrolyzers. Herein, we report a highly active and stable anode material for oxygen evolving reaction (OER) in mild-pH conditions based on cobalt oxide-nanoparticles embedded into a poly(pyrrole-alkylammonium) matrix (denoted PPN+-CoOx). Examples of hybrid materials combining metal oxide nanoparticles as OER catalysts within a polymer film are still rare. However, they are very promising to control the formation and the size of metal particles in view to enhance the electrochemically active surface area and thus the electrocatalytic performances. Our strategy consists in electroprecipitating Co0 nanoparticles by reduction of an anionic cobalt oxalate complex into the cationic PPN+ film, the latter being previously deposited onto an electrode surface by electropolymerization. The Co0 nanoparticles within the composite are then partially in-situ oxidized under air exposure into CoO, and then finally fully oxidized into CoOx by successive scans between 0 and 1.2 V vs Ag/AgCl in a borate buffer at pH 9.2. This nanocomposite material is highly structured with around 30 nm-large CoOx nanoparticles well dispersed into the polypyrrole film conferring a high OER electrocatalytic activity at near neutral pH of 9.2 with exceptional values of mass activity and turnover frequency of 3.01 A mg-1 and 0.46 s-1 respectively, at an overpotential of 0.61 V and with a cobalt loading of 1.34 µg cm-2. These performances place the PPN+-CoOx electrode among the most active anodes described in the literature employing cobalt oxide under mild pH conditions. In addition, when the PPN+-CoOx material is electrodeposited on carbon paper with a higher roughness than a simple carbon electrode, the physisorption of the film on the electrode is considerably enhanced resulting in a stable catalytic current over more than 43 h. Post electrolysis characterizations by SEM and EDX confirm the integrity of the PPN+-CoOx material after long hours of electrocatalysis. This demonstrates the beneficial role of the polypyrrole matrix in the achievement of very stable and highly active anodes for water oxidation

Presentation_1_Migrant healthcare workers during COVID-19: bringing an intersectional health system-related approach into pandemic protection. A German case study.PPTX

IntroductionMigrant healthcare workers played an important role during the COVID-19 pandemic, but data are lacking especially for high-resourced European healthcare systems. This study aims to research migrant healthcare workers through an intersectional health system-related approach, using Germany as a case study.MethodsAn intersectional research framework was created and a rapid scoping study performed. Secondary analysis of selected items taken from two COVID-19 surveys was undertaken to compare perceptions of national and foreign-born healthcare workers, using descriptive statistics.ResultsAvailable research is focused on worst-case pandemic scenarios of Brazil and the United Kingdom, highlighting racialised discrimination and higher risks of migrant healthcare workers. The German data did not reveal significant differences between national-born and foreign-born healthcare workers for items related to health status including SARS-CoV-2 infection and vaccination, and perception of infection risk, protective workplace measures, and government measures, but items related to social participation and work conditions with higher infection risk indicate a higher burden of migrant healthcare workers.ConclusionsCOVID-19 pandemic policy must include migrant healthcare workers, but simply adding the migration status is not enough. We introduce an intersectional health systems-related approach to understand how pandemic policies create social inequalities and how the protection of migrant healthcare workers may be improved.</p

Production of proinflammatory cytokines and myocardial dysfunction after arterial switch operation in neonates with transposition of the great arteries

SCOPUS: ar.jinfo:eu-repo/semantics/publishe