Application of Some Proteome Analysis Techniques in Animal Reproduction

This chapter focuses on the application of proteome analysis techniques to animal reproduction and provides general information on one-dimensional (1D) and two-dimensional (2D) electrophoresis, chromatographic methods, and mass spectrometer (MS), widely used in proteomics studies. Proteome consists of an entire complement of proteins expressed by a cell, tissue, or organism. Proteomics reveals functions of proteins encoded in the genome. These functions include posttranslational modifications (PTMs) and alterations in the protein synthesis. Animal reproduction takes a key role in livestock, and increasing the reproduction rate in flocks plays an important role in livestock management. Studying the proteins related to reproduction could guide on how to increase fertility. Recent studies addressed the proteome constitution of both male and female reproductive system. Follicular fluid, endometrium, and ovary proteins were analyzed in females by proteomics study, while in males, sperm proteomics was more focused. Information obtained on this issue is also beneficial for the development of reproductive technologies such as in vitro fertilization and embryo transfer. Strategies to increase fertility in animals can be revealed by proteomic studies, and a more profound knowledge on proteomics may become helpful to develop and enhance the efficiency of reproductive technologies

Ganglion Impar block improves neuropathic pain in coccygodynia: A preliminary report

Aim of the study To define the effectiveness of ganglion Impar block in improving neuropathic pain. Materials and methods Patients who had pain around the coccyx for more than three months and did not respond to conservative treatment were included in this study. All the patients underwent fluoroscopy guided transsacrococcygeal ganglion Impar block with injecting 3 mL of 0.5% bupivacaine, 2 mL saline, and 1 mL (40 mg) of methylprednisolone. Patients were evaluated with visual analog scale (VAS) for pain, Leeds assessment of neuropathic symptoms and signs scale (LANSS) for neuropathic pain, Beck depression Inventory (BDI) for mood and Short-form 12 (SF-12) for quality of life before, 1 month 3 months and 6 months after the injection. Patients’ painless sitting duration was also recorded. Results A total of 28 patients were included in the final analyses. VAS and LANSS scores improved significantly throughout the follow-up periods. BDI scores also improved while SF-12 scores did not show significant changes. Painless sitting period of the patients’ improved significantly. Conclusions Ganglion Impar block is effective in decreasing the neuropathic component of chronic coccygodynia. This improves painless sitting in patients but its reflections on quality of life is not clear

Clinical and Laboratory Characteristics of Hyperprolactinemia in Children and Adolescents: National Survey

Conclusion: We present the largest cohort of children and adolescents with hyperprolactinemia in the literature to date. Hyperprolactinemia is more common in females and cabergoline is highly effective and practical to use in adolescents, due to its biweekly dosing. Indications for surgery in pediatric cases need to be revised

The relationship between the conflict management strategies and ethical leadership behaviours of nurse managers perceived by nurses

Aim: The aims of this study were to determine nurse managers' level of ethical leadership perceived by nurses and to examine its relationship with conflict management strategies. Background: Ethical leadership is about how managers use their power in their decisions and actions, and its source is based on moral and ethical authority. Therefore, it is important to understand the impact of ethical behaviours of nurse managers in the work environment on determining conflict management strategies. Method: The data of this descriptive, correlational, and cross-sectional study were collected face-to-face from 285 nurses between April–June 2019. The data collection instruments included the introductory information form, the Ethical Leadership Scale, and The Rahim Organizational Conflict Inventory-II. Results: Ethical leadership scores perceived by nurses in nurse managers were found 3.78, and the highest score was obtained from the behavioural ethics subscale (3.81 ±.91). In conflict management strategies perceived by nurses, it was determined that they got highest scores from collaborating style (3.76 ±.90) and lowest scores from competing style (2.90 ±.94). There was no significant relationship only between ethical leadership and its subscales and competing (r: −.038/−.041, p >.05). In other subscales, there were positive, moderate, and highly significant relationships (r:.466–.747, p <.001). The rate of explanatoriness of communicative ethics subscale in conflict management strategies ranged from 22.3% to 58.0%. Conclusions: The study shows that communicative ethics subscale significantly affects the conflict management strategies of nurse managers. Therefore, it is important for nurse managers to communicate bilaterally and be a role model for nurses. Implications for Nursing Management: Ethical leaders value trust and respect in their interactions with employees and reflect what appropriate behaviour to the situation is. Nurses who perceive that their managers demonstrate ethical leadership behaviours will also evaluate their role in conflict resolution. On the other hand, the power-based, aggressive, noncompromising domination approach that the person imposes on others is not associated with the ethical leadership perception towards their managers by nurses and is a strategy that should not be preferred. Therefore, nurse managers who use appropriate conflict management strategies were seen as a role model by nurses. © 2022 John Wiley & Sons Ltd

Examining the hand hygiene beliefs and practices of nursing students and the effectiveness of their handwashing behaviour

Ozturk, Huri/0000-0003-1680-6810WOS: 000564479000001PubMed: 32730649Aims and objectives To examine the hand hygiene beliefs and practices of Turkish nursing students and the effectiveness of their handwashing. Background Handwashing is the most important part of preventing cross-infection, but there is a considerable amount of evidence that shows that the handwashing technique of nurses and nursing students is not always very effective. Design/Methods This research was carried out in two stages and is type of descriptive, analytical and observational. the study comprised 563 students from a Faculty of Nursing. Besides evaluating the nursing students' sociodemographic data, we assessed their beliefs and hand hygiene practices using a Hand Hygiene Beliefs Scale (HHBS) and Hand Hygiene Practices Inventory (HHPI) and also used with "Derma LiteCheck" device to assess how effectively they washed their hands. the STROBE checklist was used. Results the handwashing frequency of the female students was significantly higher. the scores on both the HHBS and the HHPI were significantly higher among the female students compared to the males. the dirtiest areas of the hand were determined as nails (nail beds, beneath the nails), skin between the fingers and fingertips. Conclusions the results of our study indicate that although student nurses have positive perceptions about hand hygiene and appear to have developed good habits, the effectiveness of their handwashing is poor. Relevance to clinical practice It is of great importance that UV light and florescent gel are made readily available both at nursing schools and in hospitals and other health institutions since these elements are useful in providing immediate visual feedback for a full assessment of handwashing behaviour.Ege University Scientific Research Projects FundEge University [2018-HEF003]This research was supported by Ege University Scientific Research Projects Fund (Project acceptance number: 2018-HEF003)