Interaction of C-Terminal Truncated Human αA-Crystallins with Target Proteins

Significant portion of alphaA-crystallin in human lenses exists as C-terminal residues cleaved at residues 172, 168, and 162. Chaperone activity, determined with alcohol dehydrogenase (ADH) and betaL-crystallin as target proteins, was increased in alphaA(1-172) and decreased in alphaA(1-168) and alphaA(1-162). The purpose of this study was to show whether the absence of the C-terminal residues influences protein-protein interactions with target proteins.Our hypothesis is that the chaperone-target protein binding kinetics, otherwise termed subunit exchange rates, are expected to reflect the changes in chaperone activity. To study this, we have relied on fluorescence resonance energy transfer (FRET) utilizing amine specific and cysteine specific fluorescent probes. The subunit exchange rate (k) for ADH and alphaA(1-172) was nearly the same as that of ADH and alphaA-wt, alphaA(1-168) had lower and alphaA(1-162) had the lowest k values. When betaL-crystallin was used as the target protein, alphaA(1-172) had slightly higher k value than alphaA-wt and alphaA(1-168) and alphaA(1-162) had lower k values. As expected from earlier studies, the chaperone activity of alphaA(1-172) was slightly better than that of alphaA-wt, the chaperone activity of alphaA(1-168) was similar to that of alphaA-wt and alphaA(1-162) had substantially decreased chaperone activity.Cleavage of eleven C-terminal residues including Arg-163 and the C-terminal flexible arm significantly affects the interaction with target proteins. The predominantly hydrophilic flexible arm appears to be needed to keep the chaperone-target protein complex soluble

Not Available

Not AvailableA detailed investigation on mass mortality of fishes was conducted in a small tropical reservoir- Derjang (20o50’32.0”N, 85o01’14.8″E), Odisha, India. Mortality mostly occurred in Systomus sarana followed by Labeo rohita, Cirrhinus mrigala, Labeo catla, Ompok bimaculatus, Labeo calbasu and Mastacembelus armatus. During 20 days of disease occurrence in May–June 2019, a total of about 3000 kg of fish died. The clinical signs in Cyprinid group were haemorrhagic spots, ulcerative lesions, rotten and pale patches in gills due to septicemic disease whereas haemorrhagic spots were the only prominent symptoms observed in Silurid group. Bacteriological isolation and identification through conventional and molecular techniques revealed that Klebsiella pneumoniae was the most common pathogen recovered from S. sarana, C. mrigala and O. bimaculatus. Further Aeromonas hydrophila, Acinetobacter baumannii were isolated from L. rohita and L. catla respectively. The role of these pathogens for this disease outbreak in multiple fish species is discussed in perspective of environmental factors. Sudden environmental alternation by the supercyclone Fani (3rd May 2019) on the coastal part of Odisha might have played a key role to translate the aquatic bacteria into the virulent infective pathogens. In the experimental challenge study, isolated bacteria showed pathogenicity in respective hosts as that in the reservoir. Thus this further revealed both bacteria and fish specific virulency with a variation in LD50 values. All the gram negative bacterial isolates were found to resist ampicillin and amoxicillin-clavulanic acid and most of them were TEM gene positive. However, the bacteria were found to be susceptible to the rest of the nineteen antibiotics. These findings suggested that the sudden cyclone is an enormous threat to reservoir aquaculture, and should be taken into consideration before breeding, stocking and harvesting of fishes.Not Availabl

In vitro leishmanicidal activity of Tityus discrepans scorpion venom

Item does not contain fulltex