14 research outputs found
Kennesaw State University Percussion Ensemble, Spring Concert
KSU School of Music presents Percussion Ensemble\u27s Spring Concert.https://digitalcommons.kennesaw.edu/musicprograms/1251/thumbnail.jp
An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia
To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. However, due to their intrinsic resistance to many antibiotics only selected antibiotic-resistance markers can be used in members of the genus Burkholderia, including the Burkholderia cepacia complex (Bcc). Here we describe the construction of improved antibiotic-resistance cassettes that specify resistance to kanamycin, chloramphenicol or trimethoprim effectively in the Bcc and related species. These were then used in combination with and/or to construct a series enhanced suicide vectors, pSHAFT2, pSHAFT3 and pSHAFT-GFP to facilitate effective allelic replacement in the Bcc. Validation of these improved suicide vectors was demonstrated by the genetic inactivation of selected genes in the Bcc species Burkholderia cenocepacia and B. lata, and in the non-Bcc species, B. thailandensis
Expert range maps of global mammal distributions harmonised to three taxonomic authorities
AimComprehensive, global information on species' occurrences is an essential biodiversity variable and central to a range of applications in ecology, evolution, biogeography and conservation. Expert range maps often represent a species' only available distributional information and play an increasing role in conservation assessments and macroecology. We provide global range maps for the native ranges of all extant mammal species harmonised to the taxonomy of the Mammal Diversity Database (MDD) mobilised from two sources, the Handbook of the Mammals of the World (HMW) and the Illustrated Checklist of the Mammals of the World (CMW).LocationGlobal.TaxonAll extant mammal species.MethodsRange maps were digitally interpreted, georeferenced, error-checked and subsequently taxonomically aligned between the HMW (6253 species), the CMW (6431 species) and the MDD taxonomies (6362 species).ResultsRange maps can be evaluated and visualised in an online map browser at Map of Life (mol.org) and accessed for individual or batch download for non-commercial use.Main conclusionExpert maps of species' global distributions are limited in their spatial detail and temporal specificity, but form a useful basis for broad-scale characterizations and model-based integration with other data. We provide georeferenced range maps for the native ranges of all extant mammal species as shapefiles, with species-level metadata and source information packaged together in geodatabase format. Across the three taxonomic sources our maps entail, there are 1784 taxonomic name differences compared to the maps currently available on the IUCN Red List website. The expert maps provided here are harmonised to the MDD taxonomic authority and linked to a community of online tools that will enable transparent future updates and version control
Modular high-current generator for electromagnetic forming with energy recovery
A 10 kJ electromagnetic forming (EMF) modulator with energy recovery based on two resonant power modules, each containing a 4.5 kV/30-kA silicon controlled rectifier, a 1.11-mF capacitor bank and an energy recovery circuit, working in parallel to allow a maximum actuator discharge current amplitude and rate of 50 kA and 2 kA/mu s was successfully developed and tested. It can be plugged in standard single phase 230 V/16 A mains socket and the circuit is able to recover up to 32% of its initial energy, reducing the charging time of conventional EMF systems by up to 68%
DISTRIBUIÇÃO TERRITORIAL DA CANA-DE-AÇÚCAR EM RELAÇÃO AO USO DA TERRA E AO ZONEAMENTO AGROECOLÓGICO DA CANA NO ESTADO DE GOIÁS
Since last decades, Brazilian ethanol industry has gained notoriety in international agribusiness. The state of Goias, located in the Cerrado biome, is one of the most threatened by agricultural advancement and is the second largest producer of ethanol in Brazil. Before 2009, the country didn't have agricultural and environmental policies related to the territorial planning of sugarcane agro-business. Consequentially, changes in the landscape have occurred as a result of altering land usage for the cultivation of sugarcane. Therefore the objective of this study was to quantify the territorial sugarcane distribution in relation to land-use types and the Agro-Ecological Zoning of Sugarcane (AEZ Cane), both before and after the zoning implementation, in the state of Goias during the period of 2006 to 2012. Using geoprocessing techniques from the ArcGIS software, results show a 302% increase in the number of sugarcane fields from 2005-2012. During 2006-2008, agriculture was the preferred area of sugarcane expansion, and after 2010, the preference for expansion moved to pasture lands. From 2010 on (after AEZ Cane implementation), 87 % of the sugarcane was planted in suitable AEZ Cane areas and 13% was planted outside. Agricultural fields were the preferred areas for expansion in AEZ Cane, suggesting a crop substitution. Outside the zoning, expansion tended to occur in pasture lands, followed by agricultural fields and remaining native vegetation areas. In 2012, the remnants of native vegetation areas lost 22% in order for increased sugarcane expansion. The AEZ Cane provided forms to sugarcane spatial planning. But the need for monitoring must remain in order to prevent expansion outside the zoning areas.Desde as últimas décadas, a indústria sucroalcooleira brasileira vem conquistando notoriedade no cenário do agronegócio internacional. O estado de Goiás, situado no bioma Cerrado, é o segundo maior produtor de etanol do Brasil e um dos mais ameaçados pelo avanço agropecuário. Antes de 2009, o país não possuía políticas agrícolas e ambientais relacionadas ao ordenamento territorial da agroindústria da cana-de-açúcar. Por consequência, ocorreram modificações na paisagem, resultantes da substituição de outros usos para o cultivo da cana-de-açúcar. Nesse contexto, objetivou-se quantificar a distribuição territorial da cana-de-açúcar em relação ao uso da terra e ao Zoneamento Agroecológico da cana-de-açúcar (ZAE Cana), antes e após sua implementação, durante o período de 2006 e 2012 no Estado de Goiás. Utilizando o software ArcGIS, os resultados mostraram que, entre 2005 a 2012, as áreas plantadas com cana-de-açúcar cresceram 302%. Entre 2006-2008, a expansão ocorreu preferencialmente sobre as áreas de agricultura e, após 2010, sobre as de pastagem. A partir de 2010, 87% do plantio foi realizado nas áreas aptas do ZAE Cana, e 13%, fora do ZAE Cana. No ZAE Cana, a expansão ocorreu sobre as áreas de agricultura. Fora do ZAE Cana, o plantio incidiu preferencialmente sobre as áreas de pastagem, seguido pelas de agricultura e de remanescentes de vegetação nativa. Em 2012, as áreas remanescentes de vegetação nativa perderam 22% de sua área para a expansão da cana-de-açúcar. O ZAE Cana proporcionou formas para o ordenamento territorial da cana-de-açúcar, mas ainda é necessário que haja monitoramento para evitar a expansão fora do zoneamento.