Passive phloem loading and long-distance transport in a synthetic tree-on-a-chip

Vascular plants rely on differences of osmotic pressure to export sugars from regions of synthesis (mature leaves) to sugar sinks (roots, fruits). In this process, known as M\"unch pressure flow, the loading of sugars from photosynthetic cells to the export conduit (the phloem) is crucial, as it sets the pressure head necessary to power long-distance transport. Whereas most herbaceous plants use active mechanisms to increase phloem concentration above that of the photosynthetic cells, in most tree species, for which transport distances are largest, loading seems to occur via passive symplastic diffusion from the mesophyll to the phloem. Here, we use a synthetic microfluidic model of a passive loader to explore the nonlinear dynamics that arise during export and determine the ability of passive loading to drive long-distance transport. We first demonstrate that in our device, phloem concentration is set by the balance between the resistances to diffusive loading from the source and convective export through the phloem. Convection-limited export corresponds to classical models of M\"unch transport, where phloem concentration is close to that of the source; in contrast, diffusion-limited export leads to small phloem concentrations and weak scaling of flow rates with the hydraulic resistance. We then show that the effective regime of convection-limited export is predominant in plants with large transport resistances and low xylem pressures. Moreover, hydrostatic pressures developed in our synthetic passive loader can reach botanically relevant values as high as 10 bars. We conclude that passive loading is sufficient to drive long-distance transport in large plants, and that trees are well suited to take full advantage of passive phloem loading strategies

Linkage among melanin biosynthetic mutations in Cochliobolus heterostrophus

Melanin is synthesized by C. heterostrophus from acetate via pentaketide and several dihydroxynaphthalene intermediates (Tanaka et al. 1991 Mycol. Res. 95:49-56), as it is for certain other fungi (Bell and Wheeler 1986 Ann. Rev. Phytopathol. 24:411-451; Kubo et al. 1989 Exp. Mycol 13:77-84; Chumley and Valent 1990 Mol. Plant-Microbe Int. 3:135-143). Previously, five melanin deficient mutants of C. heterostrophus were analyzed by Tanaka et al. (Mycol. Res. 95:49-56), who were unable to establish complete linkage relationships because three of the mutations (alb1, alb3, and brn1) showed no recombination when crossed to each other, and were unlinked to the other two (sal1 and pgr1), which mapped about 12 cM apart. A sixth color mutation, scr1, represented a third linkage group, but there was no evidence of its involvement in melanin biosynthesis. Independently, we have recovered six melanin-deficient mutants, one of which (alb1, Leach et al. 1982 J. Gen. Microbiol. 128:1719-1729) was included in the study of Tanaka et al. and maps to chromosome 1 on the C. heterostrophus RFLP map (Tzeng et al. 1992 Genetics 130:81-96). We report here that our remaining five melanin-deficient mutants [crm1 (light cream), crm2 (dark cream), brn1 (brown), rsy1 (rose), and probably gra3 (gray)] are linked to, but are not allelic with, alb1 (white) and constitute a gene cluster on chromosome

Pharmacists Play Role in Pharmacogenomics Clinical Services

They should consider many factors and use clear guidance on predicting phenotypes from genotypes to intervene optimally based on specific drug-gene interactions

Phloem sap and leaf δ13C, carbohydrates, and amino acid concentrations in Eucalyptus globulus change systematically according to flooding and water deficit treatment

Phloem is a central conduit for the distribution of photoassimilate, nutrients, and signals among plant organs. A revised technique was used to collect phloem sap from small woody plants in order to assess changes in composition induced by water deficit and flooding. Bled phloem sap δ13C and sugar concentrations were compared to δ13C of bulk material, soluble carbon extracts, and the neutral sugar fraction from leaves. Amino acid composition and inorganic ions of the phloem sap was also analysed. Quantitative, systematic changes were detected in phloem sap composition and δ13C in response to altered water availability. Phloem sap δ13C was more sensitive to changes of water availability than the δ13C of bulk leaf, the soluble carbon fraction, and the neutral soluble fraction of leaves. Changes in water availability also resulted in significant changes in phloem sugar (sucrose and raffinose), inorganic nutrient (potassium), and amino acid (phenylalanine) concentrations with important implications for the maintenance of phloem function and biomass partitioning. The differences in carbohydrate and amino acid composition as well as the δ13C in the phloem, along with a new model system for phloem research, offer an improved understanding of the phloem-mediated signal, nutrient, and photoassimilate transduction in relation to water availability