A mixed-fidelity numerical study for Fan-Distortion interaction

Inlet distortion often occurs under off-design conditions when a flow separates within an intake and this unsteady phenomenon can seriously impact fan performance. Fan–distortion interaction is a highly unsteady aerodynamic process into which high-fidelity simulations can provide detailed insights. However, due to limitations on the computational resource, the use of an eddy resolving method for a fully resolved fan calculation is currently infeasible within industry. To solve this problem, a mixed-fidelity computational fluid dynamics method is proposed. This method uses the large Eddy simulation (LES) approach to resolve the turbulence associated with separation and the immersed boundary method (IBM) with smeared geometry (IBMSG) to model the fan. The method is validated by providing comparisons against the experiment on the Darmstadt Rotor, which shows a good agreement in terms of total pressure distributions. A detailed investigation is then conducted for a subsonic rotor with an annular beam-generating inlet distortion. A number of studies are performed in order to investigate the fan's influence on the distortions. A comparison to the case without a fan shows that the fan has a significant effect in reducing distortions. Three fan locations are examined which reveal that the fan nearer to the inlet tends to have a higher pressure recovery. Three beams with different heights are also tested to generate various degrees of distortion. The results indicate that the fan can suppress the distortions and that the recovery effect is proportional to the degree of inlet distortion.</jats:p

Toward Future Installations: Mutual Interactions of Short Intakes With Modern High Bypass Fans

In this paper, we investigate the coupled interaction between a new short intake design with a modern fan in a high-bypass ratio civil engine, specifically under the off-design condition of high incidence. The interaction is expected to be much more significant than that on a conventional intake. The performance of both the intake-alone and rotor-alone configurations are examined under isolation. Subsequently, a comprehensive understanding on the two-way interaction between intake and fan is presented. This includes the effect of fan on intake angles of attack (AoA) tolerance (FoI) and the effect of circumferential and radial flow distortion induced by the intake on the fan performance (IoF). In the FoI scenario, the rotor effectively redistributes the mass flow at the fan-face. The AoA tolerance of the short-intake design has increased by ≈4 deg when compared with the intake-alone configuration. Dynamic nature of distortion due to shock unsteadiness has been quantified. ST plots and power spectral density (PSD) of pressure fluctuations show the existence of a spectral gap between the shock unsteadiness and blade passing, with almost an order of magnitude difference in the corresponding frequencies. In the IoF scenario, both the “large” (O(360 deg)) and “small” scale distortion (O(10–60 deg)) induced by the intake results in a non-uniform inflow to the rotor. Sector analysis reveals a substantial variation in the local operating condition of the fan as opposed to its steady characteristic. Streamline curvature, upwash, and wake thickening are identified to be the three key factors affecting the fan performance. These underlying mechanisms are discussed in detail to provide further insights into the physical understanding of the fan-intake interaction. In addition to the shock-induced separation on the intake lip, the current study shows that shorter intakes are much more prone to the upwash effect at higher AoA. Insufficient flow straightening along the engine axis is reconfirmed to be one of the limiting factors for the short-intake design

Toward Future Installations: Mutual Interactions of Short Intakes With Modern High Bypass Fans

In this paper, we investigate the coupled interaction between a new short intake design with a modern fan in a high-bypass ratio civil engine, specifically under the off-design condition of high incidence. The interaction is expected to be much more significant than that on a conventional intake. The performance of both the intake-alone and rotor-alone configurations are examined under isolation. Subsequently, a comprehensive understanding on the two-way interaction between intake and fan is presented. This includes the effect of fan on intake angles of attack (AoA) tolerance (FoI) and the effect of circumferential and radial flow distortion induced by the intake on the fan performance (IoF). In the FoI scenario, the rotor effectively redistributes the mass flow at the fan-face. The AoA tolerance of the short-intake design has increased by ≈4 deg when compared with the intake-alone configuration. Dynamic nature of distortion due to shock unsteadiness has been quantified. ST plots and power spectral density (PSD) of pressure fluctuations show the existence of a spectral gap between the shock unsteadiness and blade passing, with almost an order of magnitude difference in the corresponding frequencies. In the IoF scenario, both the “large” (O(360 deg)) and “small” scale distortion (O(10–60 deg)) induced by the intake results in a non-uniform inflow to the rotor. Sector analysis reveals a substantial variation in the local operating condition of the fan as opposed to its steady characteristic. Streamline curvature, upwash, and wake thickening are identified to be the three key factors affecting the fan performance. These underlying mechanisms are discussed in detail to provide further insights into the physical understanding of the fan-intake interaction. In addition to the shock-induced separation on the intake lip, the current study shows that shorter intakes are much more prone to the upwash effect at higher AoA. Insufficient flow straightening along the engine axis is reconfirmed to be one of the limiting factors for the short-intake design

Fan-Intake interaction under high incidence

In this paper, we present an extensive numerical study on the interaction between the downstream fan and the flow separating over an intake under high incidence. The objectives of this investigation are twofold: (a) to gain qualitative insight into the mechanism of fan–intake interaction and (b) to quantitatively examine the effect of the proximity of the fan on the inlet distortion. The fan proximity is altered using the key design parameter, L/D, where D is the diameter of the intake, and L is the distance of the fan from the intake lip. Both steady and unsteady Reynolds-averaged numerical simulations (RANS) were carried out. For the steady calculations, a low-order fan model has been used, while a full 3D geometry has been used for the unsteady RANS. The numerical methodology is also thoroughly validated against the measurements for the intake-only and fan-only configurations on a high bypass ratio turbofan intake and fan, respectively. To systematically study the effect of fan on the intake separation and explore the design criteria, a simplified intake–fan configuration has been considered. In this fan–intake model, the proximity of the fan to the intake separation (L/D) can be conveniently altered without affecting other parameters. The key results indicate that, depending on L/D, the fan has either suppressed the level of the postseparation distortion or increased the separation-free operating range. At the lowest L/D (∼0.17), around a 5 deg increase in the separation-free angle of incidence was achieved. This delay in the separation-free angle of incidence decreased with increasing L/D. At the largest L/D (∼0.44), the fan was effective in suppressing the postseparation distortion rather than entirely eliminating the separation. Isentropic Mach number distribution over the intake lip for different L/D's revealed that the fan accelerates the flow near the casing upstream of the fan face, thereby decreasing the distortion level in the immediate vicinity. However, this acceleration effect decayed rapidly with increasing upstream distance from the fan-face.</jats:p

Distributed Roughness Effects on Transitional and Turbulent Boundary Layers

© 2017 Springer Science+Business Media B.V. A numerical investigation is carried out to study the transition of a subsonic boundary layer on a flat plate with roughness elements distributed over the entire surface. Post-transition, the effect of surface roughness on a spatially developing turbulent boundary layer (TBL) is explored. In the transitional regime, the onset of flow transition predicted by the current simulations is in agreement with the experimentally based correlations proposed in the literature. Transition mechanisms are shown to change significantly with the increasing roughness height. Roughness elements that are inside the boundary layer create an elevated shear layer and alternating high and low speed streaks near the wall. Secondary sinuous instabilities on the streaks destabilize the shear layer promoting transition to turbulence. For the roughness topology considered, it is observed that the instability wavelengths are governed by the streamwise and spanwise spacing between the roughness elements. In contrast, the roughness elements that are higher than the boundary layer create turbulent wakes in their lee. The scale of instability is much shorter and transition occurs due to the shedding from the obstacles. Post-transition, in the spatially developing TBL, the velocity defect profiles for both the smooth and rough walls collapsed when non dimensionalized in the outer units. However, when compared to the smooth wall, deviation in the Reynolds stresses are observable in the outer layer; the deviation being higher for the larger roughness elements

The Human Fungal Pathogen Cryptococcus neoformans Escapes Macrophages by a Phagosome Emptying Mechanism That Is Inhibited by Arp2/3 Complex-Mediated Actin Polymerisation

The lysis of infected cells by disease-causing microorganisms is an efficient but risky strategy for disseminated infection, as it exposes the pathogen to the full repertoire of the host's immune system. Cryptococcus neoformans is a widespread fungal pathogen that causes a fatal meningitis in HIV and other immunocompromised patients. Following intracellular growth, cryptococci are able to escape their host cells by a non-lytic expulsive mechanism that may contribute to the invasion of the central nervous system. Non-lytic escape is also exhibited by some bacterial pathogens and is likely to facilitate long-term avoidance of the host immune system during latency. Here we show that phagosomes containing intracellular cryptococci undergo repeated cycles of actin polymerisation. These actin ‘flashes’ occur in both murine and human macrophages and are dependent on classical WASP-Arp2/3 complex mediated actin filament nucleation. Three dimensional confocal imaging time lapse revealed that such flashes are highly dynamic actin cages that form around the phagosome. Using fluorescent dextran as a phagosome membrane integrity probe, we find that the non-lytic expulsion of Cryptococcus occurs through fusion of the phagosome and plasma membranes and that, prior to expulsion, 95% of phagosomes become permeabilised, an event that is immediately followed by an actin flash. By using pharmacological agents to modulate both actin dynamics and upstream signalling events, we show that flash occurrence is inversely related to cryptococcal expulsion, suggesting that flashes may act to temporarily inhibit expulsion from infected phagocytes. In conclusion, our data reveal the existence of a novel actin-dependent process on phagosomes containing cryptococci that acts as a potential block to expulsion of Cryptococcus and may have significant implications for the dissemination of, and CNS invasion by, this organism.\ud \u

Lectin-like bacteriocins from pseudomonas spp. utilise D-rhamnose containing lipopolysaccharide as a cellular receptor

Lectin-like bacteriocins consist of tandem monocot mannose-binding domains and display a genus-specific killing activity. Here we show that pyocin L1, a novel member of this family from Pseudomonas aeruginosa, targets susceptible strains of this species through recognition of the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide that is predominantly a homopolymer of d-rhamnose. Structural and biophysical analyses show that recognition of CPA occurs through the C-terminal carbohydrate-binding domain of pyocin L1 and that this interaction is a prerequisite for bactericidal activity. Further to this, we show that the previously described lectin-like bacteriocin putidacin L1 shows a similar carbohydrate-binding specificity, indicating that oligosaccharides containing d-rhamnose and not d-mannose, as was previously thought, are the physiologically relevant ligands for this group of bacteriocins. The widespread inclusion of d-rhamnose in the lipopolysaccharide of members of the genus Pseudomonas explains the unusual genus-specific activity of the lectin-like bacteriocins

Relative effects of sensory modalities and importance of fatty acid sensitivity on fat perception in a real food model

Fat can be perceived through mouthfeel, odour and taste, but the influence of these modalities on fat perception remains undefined. Fatty acids are stimuli. Individual’s sensitivity to fatty acids varies. Studies show association between fatty acid sensitivity, dietary intake and BMI, but results are conflicting. Therefore, this study examined this association, and the effects of modalities on fat perception. Two sub-studies conducted. In Study 1 (n=46), fat intensity was assessed by milk/cream mixtures varying by five fat levels. Fat intensity was rated under four conditions: mouthfeel-odour masked, mouthfeel masked, odour masking and no masking. Mouthfeel masking was achieved using thickener and paraffin, odour masking using nose-clips. Fatty acid sensitivity was measured by 3-AFC-staircase method using milk containing oleic acid (0.31-31.4mM). In Study 2 (n=51), more fat levels were added in fat intensity rating. A 2-AFC discrimination test was used to confirm whether fat levels could be distinguished. In the sensitivity test, a wider range of oleic acid was included. Fat intensity was rated higher without nose-clips (p<0.0001), implying that odour increased fat perception. Samples with mouthfeel-masked were rated higher, showing that increased viscosity and lubricity enhanced fat perception (p<0.0001). Participants could distinguish fat levels based on “taste” in rating tests and 2-AFC-tests. Participants were divided into high/medium/low-sensitivity groups. No significant difference found in fat intensity between groups, however, high-sensitivity group discriminated more fat levels. No association between sensitivity groups, nutrient intake or BMI found

Relationships of dietary patterns with body composition in older adults differ by gender and PPAR-γ Pro12Ala genotype

Dietary patterns may better capture the multifaceted effects of diet on body composition than individual nutrients or foods. The objective of this study was to investigate the dietary patterns of a cohort of older adults, and examine relationships of dietary patterns with body composition. The influence of a polymorphism in the peroxisome proliferator-activated receptor-γ (PPAR-γ) gene was considered. The Health, Aging and Body Composition (Health ABC) Study is a prospective cohort study of 3,075 older adults. Participants’ body composition and genetic variation were measured in detail. Food intake was assessed with a semi-quantitative food frequency questionnaire (Block Dietary Data Systems, Berkeley, CA), and dietary patterns of 1,809 participants with complete data were derived by cluster analysis. Six clusters were identified, including a ‘Healthy foods’ cluster characterized by higher intake of low-fat dairy products, fruit, whole grains, poultry, fish and vegetables. An interaction was found between dietary patterns and PPAR-γ Pro12Ala genotype in relation to body composition. While Pro/Pro homozygous men and women in the ‘Healthy foods’ cluster did not differ significantly in body composition from those in other clusters, men with the Ala allele in the ‘Healthy foods’ cluster had significantly lower levels of adiposity than those in other clusters. Women with the Ala allele in the ‘Healthy foods’ cluster differed only in right thigh intermuscular fat from those in other clusters. Relationships between diet and body composition in older adults may differ by gender and by genetic factors such as PPAR-γ Pro12Ala genotype

The role of soluble fiber intake in patients under highly effective lipid-lowering therapy

<p>Abstract</p> <p>Background</p> <p>It has been demonstrated that statins can increase intestinal sterol absorption. Augments in phytosterolemia seems related to cardiovascular disease.</p> <p>Objective</p> <p>We examined the role of soluble fiber intake in endogenous cholesterol synthesis and in sterol absorption among subjects under highly effective lipid-lowering therapy.</p> <p>Design</p> <p>In an open label, randomized, parallel-design study with blinded endpoints, subjects with primary hypercholesterolemia (n = 116) were assigned to receive during 12 weeks, a daily dose of 25 g of fiber (corresponding to 6 g of soluble fibers) plus rosuvastatin 40 mg (n = 28), rosuvastatin 40 mg alone (n = 30), sinvastatin 40 mg plus ezetimibe 10 mg plus 25 g of fiber (n = 28), or sinvastatin 40 mg plus ezetimibe 10 mg (n = 30) alone.</p> <p>Results</p> <p>The four assigned therapies produced similar changes in total cholesterol, LDL-cholesterol, and triglycerides (p < 0.001 vs. baseline) and did not change HDL-cholesterol. Fiber intake decreased plasma campesterol (p < 0.001 vs. baseline), particularly among those patients receiving ezetimibe (p < 0.05 vs. other groups), and β-sitosterol (p = 0.03 vs. baseline), with a trend for lower levels in the group receiving fiber plus ezetimibe (p = 0.07). Treatment with rosuvastatin alone or combined with soluble fiber was associated with decreased levels of desmosterol (p = 0.003 vs. other groups). Compared to non-fiber supplemented individuals, those treated with fibers had weight loss (p = 0.04), reduced body mass index (p = 0.002) and blood glucose (p = 0.047).</p> <p>Conclusion</p> <p>Among subjects treated with highly effective lipid-lowering therapy, the intake of 25 g of fibers added favorable effects, mainly by reducing phytosterolemia. Additional benefits include improvement in blood glucose and anthropometric parameters.</p