PHARMACOTHERAPEUTIC EFFICACY OF THE PREPARATION «BALIZ-2» IN TREATMENT OF INFLAMMATORY DISEASES

In given article efficiency of use in clinical practice of a preparation «BaIiz-2» is considered. The authors proved its pharmacotherapeutic value in treatment of bacterial vaginosis. It is proved, that besides clinical effect, the preparation has immunomodulatory effect. The preparation «Baliz-2» possesses antimicrobic, antiinflammatory, and immunomodulatory effect

Predictive and prognostic significance of mRNA expression and DNA copies aberrations of ERCC1, RRM1, TOP1, TOP2A, TUBB3, TYMS, and GSTP1 genes in patients with breast cancer

Increasingly, many researchers are focusing on the sensitivity in breast tumors (BC) to certain chemotherapy drugs and have personalized their research based on the assessment of this sensitivity. One such personalized approach is to assess the chemotherapy’s gene expression, as well as aberrations in the number of DNA copies—deletions and amplifications with the ability to have a significant effect on the gene’s activity. Thus, the aim of this work was to study the predictive and prognostic significance of the expression and chromosomal aberrations of eight chemosensitivity genes in breast cancer patients. Material and methods. The study involved 97 patients with luminal B breast cancer IIB–IIIB stages. DNA and RNA were isolated from samples of tumor tissue before and after treatment. Microarray analysis was performed for all samples on high-density microarrays (DNA chips) of Affymetrix (USA) CytoScanTM HD Array and Clariom™ S Assay, human. Detection of expression level of seven chemosensitivity genes—RRM1, ERCC1, TOP1, TOP2a, TUBB3, TYMS, and GSTP1—was performed using PCR real-time (RT-qPCR). Results. The expression of the RRM1 (AC scheme), TOP2a, TYMS, and TUBB3 genes in patients with an objective response to treatment (complete and partial regression) is higher than in patients with stabilization and progression (p < 0.05). According to our results, the presence of a high level of GSTP1 in a tumor biopsy is associated with the low efficiency of the NAC CP scheme (p = 0.05). The presence of RRM1 deletion is associated with complete and partial regression, as for the TOP1 and TUBB3 genes (p < 0.05). Higher rates of metastatic survival are associated with a high level of expression and amplification of the GSTP1 gene (log-rank test p = 0.02 and p = 0.05). Conclusion. Thus, a complex assessment of the chemotherapy’s gene expression is important not only for understanding the heterogeneity and molecular biology of breast cancer but also to obtain a more accurate disease prognosis

Electronic waste, an environmental problem exported to developing countries: The GOOD, the BAD and the UGLY

Electronic waste (e-waste) is a rapidly developing environmental problem particularly for the most developed countries. There are technological solutions for processing it, but these are costly, and the cheaper option for most developed countries has been to export most of the waste to less developed countries. There are various laws and policies for regulating the processing of e-waste at different governance scales such as the international Basel Convention, the regional Bamoko Convention, and various national laws. However, many of the regulations are not fully implemented and there is substantial financial pressure to maintain the jobs created for processing e-waste. Mexico, Brazil, Ghana Nigeria, India, and China have been selected for a more detailed study of the transboundary movements of e-waste. This includes a systematic review of existing literature, the application of the Driver, Pressure, State, Impact, Response (DPSIR) framework for analysing complex problems associated with social ecological systems, and the application of the Life Cycle Assessment (LCA) for evaluating the environmental impact of electronic devices from their manufacture through to their final disposal. Japan, Italy, Switzerland, and Norway have been selected for the LCA to show how e-waste is diverted to developing countries, as there is not sufficient data available for the assessment from the selected developing countries. GOOD, BAD and UGLY outcomes have been identified from this study: the GOOD is the creation of jobs and the use of e-waste as a source of raw materials; the BAD is the exacerbation of the already poor environmental conditions in developing countries; the UGLY is the negative impact on the health of workers processing e-waste due to a wide range of toxic components in this waste. There are a number of management options that are available to reduce the impact of the BAD and the UGLY, such as adopting the concept of a circular economy, urban mining, reducing loopholes and improving existing policies and regulations, as well as reducing the disparity in income between the top and bottom of the management hierarchy for e-waste disposal. The overarching message is a request for developed countries to help developing countries in the fight against e-waste, rather than exporting their environmental problems to these poorer regions.info:eu-repo/semantics/publishedVersio

Abundance and Taxonomic Composition of Bacterioplankton in Freshwater Lake Gusinoye (Buryatia) in the Warm Water Zone of the Gusinoozerskaya Thermal Power Plant

The abundance and taxonomic composition of bacterioplankton was determined in different parts of Lake Gusinoyе, which is the largest freshwater lake in Buryatia (Russia) and is used as a cooling reservoir for the Gusinoozerskaya thermal power plant (TPP). The lake is also used as a public water supply and for commercial fishing. The chemical composition of water in the warm water discharge channel did not differ from that in the lake. The total number of bacteria in the water ranged from 0.1 to 2.5 million cells/ml; the maximum counts of bacterial cells were obtained close to the mouth of the Zagustai River, where household effluents are discharged. Seasonal dynamics showed an increase in bacterial numbers that corresponded with the spring peak of phytoplankton development in May. The maximum numbers of organotrophic bacteria were observed in the warm water discharge channel, the point of its influx into the lake and near the inflowing rivers’ mouths. There were no significant differences between the number of bacteria in the zones subjected to and those not subjected to the thermal impact of the power plant. However, total abundance and numbers of organotrophic bacteria were higher in the warm water discharge channel and in the mouth areas of the rivers. This observation suggests that a “barrier zone” with increased number and activity of microorganisms, typical of river mouths, may also be formed in the discharge channel. The patterns of bacterioplankton diversity in the parts of the lake exposed to the thermal pressure were similar. The phyla Cyanobacteria (average 28%), Actinobacteria (average 28%) and Proteobacteria (average 22%), Firmicutes (average 9%) and Bacteroidetes (average 7%) dominated. The peculiarity of the taxonomic composition of this community was the dominance of the phyla Cyanobacteria and Actinobacteria, which indicates that production and destruction processes can be intensifie

Features of the integration of two-layer metal knitwear made of titanium nickelide during the replacement of a thoracoabdominal defect in the experiment

The aim of investigation was to study experimentally the morphological features of tissue integration of two-layer titanium nickelide (TiNi) knitwear when replacing thoracoabdominal defects.Materials and methods. The experiments were carried out on 40 Wistar rats. The experimental animals were divided into two comparison groups: in Group A (n = 20) the defect was replaced using a two-layer knitted tape made of TiNi, in Group B (n = 20) a polypropylene mesh implant was used. The technique of the operation and the peculiarities of keeping the animals did not differ. Animals were taken out after 14, 30, 60 and 90 days of experiment. The macroscopic structural features at the site of implant fixation to tissues and at the sites of contact with underlying organs were studied, and the inflammatory process was assessed. The histological and electron microscopic study was carried out with an assessment of the features of tissue integration through the mesh structure of knitwear.Results. Thirty days after the surgery in four cases of Group B the appearance of the chest wall deformation at the site of implant fixation was noted, in one case the deformation site was located along the lateral edge of the abdominal wall. Among the animals of Group A no such changes were recorded. The histological and electron microscopy examination revealed that the porous structure of the TiNi wire, as well as the biomechanical and biochemical properties of the two-layer metal knitwear, ensure optimal integration of the endoprosthesis in the body tissues, forming an elastic frame close to natural. In Group B, on the contrary, the reaction of the body caused by the implanted polypropylene prosthesis was characterized by more pronounced fibrosis, and tissue integration through the mesh structure of the implant was not observed.Conclusion. Two-layer TiNi knitwear in the replacement of complex structures of the thoracoabdominal zone showed promising results, which opens up prospects for further clinical research

Enhancing gold recovery from electronic waste via lixiviant metabolic engineering in Chromobacterium violaceum

10.1038/srep02236Scientific Reports3

Численность и таксономический состав бактериопланктона в пресном озере Гусиное (Бурятия) в зоне влияния теплых вод Гусиноозерской ГРЭС

The abundance and taxonomic composition of bacterioplankton was determined in different parts of Lake Gusinoyе, which is the largest freshwater lake in Buryatia (Russia) and is used as a cooling reservoir for the Gusinoozerskaya thermal power plant (TPP). The lake is also used as a public water supply and for commercial fishing. The chemical composition of water in the warm water discharge channel did not differ from that in the lake. The total number of bacteria in the water ranged from 0.1 to 2.5 million cells/ml; the maximum counts of bacterial cells were obtained close to the mouth of the Zagustai River, where household effluents are discharged. Seasonal dynamics showed an increase in bacterial numbers that corresponded with the spring peak of phytoplankton development in May. The maximum numbers of organotrophic bacteria were observed in the warm water discharge channel, the point of its influx into the lake and near the inflowing rivers’ mouths. There were no significant differences between the number of bacteria in the zones subjected to and those not subjected to the thermal impact of the power plant. However, total abundance and numbers of organotrophic bacteria were higher in the warm water discharge channel and in the mouth areas of the rivers. This observation suggests that a “barrier zone” with increased number and activity of microorganisms, typical of river mouths, may also be formed in the discharge channel. The patterns of bacterioplankton diversity in the parts of the lake exposed to the thermal pressure were similar. The phyla Cyanobacteria (average 28%), Actinobacteria (average 28%) and Proteobacteria (average 22%), Firmicutes (average 9%) and Bacteroidetes (average 7%) dominated. The peculiarity of the taxonomic composition of this community was the dominance of the phyla Cyanobacteria and Actinobacteria, which indicates that production and destruction processes can be intensifiedОпределены численность и таксономический состав бактериопланктона в различных участках крупнейшего в Республике Бурятия пресного озера Гусиное, которое является водоемом-охладителем Гусиноозерской ГРЭС, также используется для хозяйственно-питьевого водоснабжения и в рыбохозяйственных целях. По химическому составу вод не выявлено отличий между сбросным каналом теплых вод и акваторией озера. Общая численность бактерий в воде озера колебалась от 0.1 до 2.5 млн. кл/мл, максимальные значения выявлены около устья реки Загустай, где происходит сброс очищенных сточных бытовых вод. В сезонной динамике наблюдалось увеличение численности почти на всех станциях в мае, в весенний пик развития фитопланктона. Максимальная численность органотрофных бактерий обнаружена в сбросном канале теплых вод и месте впадения сбросного канала в озеро, а также в местах вблизи впадения в озеро рек. Явных различий по показателям численности бактерий между зоной теплового влияния ГРЭС и зоной, не подверженной тепловому влиянию, не наблюдалось. Можно отметить увеличение численности микроорганизмов в термальном сбросном канале и в участках около устьев рек. Возможно, в сбросном канале образуется «барьерная зона», характерная для устьев рек и характеризующаяся увеличением численности и активности микроорганизмов. Разнообразие бактериопланктона в участках озера, подвергающихся тепловому прессу, было схожим. В составе сообщества доминировали представители филумов Cyanobacteria (в среднем 28%), Actinobacteria (в среднем 28%) и Proteobacteria (в среднем 22%), Firmicutes (в среднем 9%) и Bacteroidetes (7%). Особенностью таксономического состава сообщества было доминирование филумов Cyanobacteria и Actinobacteria, что указывает на возможную интенсификацию процессов продукции и деструкци

Electrochemical study of gold recovery from ammoniacal thiosulfate, simulating the PCBs leaching of mobile phones

[EN] The high volume of sales and the high degree of obsolescence of mobile phones, together with the reduction of the natural reserves of the metals used in the composition of their printed circuit boards (PCBs), makes the recycling of these devices economically and environmentally attractive. Moreover, the search for the reduction of toxicity levels inherent to the gold leaching processes with alternatives to cyanide, such as thiosulfate is a priority. Thus, it is necessary to search for efficient alternatives for the recovery of gold from solutions containing thiosulfate, in the presence of copper, used in the leaching of PCBs of mobile phones. One of these alternatives could be the electrochemical recovery of the metals present in solution. Thus, this study aimed to verify some variables involved in the process of recovery of gold and copper and to determine the electrochemical yield obtained for these solutions. Initially, cyclic scanning voltammetry with a rotating disk electrode (RDE) was performed to verify the electrochemical behavior of gold and copper in solution. Then, electrowinning tests were used to determine the recovery rates of these metals and to calculate the yield obtained in the process. The results showed that this electrochemical reaction is mass transport controlled, which allowed the calculation of the diffusion coefficients of the metal in solution. In real solutions, the gold fraction recovered reached a 94%, and the copper fraction recovered was 95%, applying electrode potential values of -500 mV(Ag/AgCl) and -700 mV(Ag/AgCl), respectively. The current efficiency for the gold electrowinning achieved in the experiments was lower than 3%. (c) 2017 Elsevier Ltd. All rights reserved.The authors would like to thank the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) from Brazil for financial support.Kasper, AC.; Veit, HM.; García Gabaldón, M.; Pérez-Herranz, V. (2018). Electrochemical study of gold recovery from ammoniacal thiosulfate, simulating the PCBs leaching of mobile phones. Electrochimica Acta. 259:500-509. https://doi.org/10.1016/j.electacta.2017.10.161S50050925

Экспрессионная гетерогенность генов семейства ABC-транспортеров и генов химиочувствительности в опухоли желудка, канцероматозе и метастазах в лимфатические узлы

Introduction. Metastatic tumors (particularly gastric cancer) have been found to be characterized by heterogeneity between the primary tumor and metastases. This type of heterogeneity comes to the fore when treating primary-metastatic forms of tumor and is an important reason for the low effectiveness of their treatment. In this regard, comparative analysis of ABC-transporter gene expression and chemosensitivity genes will allow to characterize to a certain extent the resistance and sensitivity of primary tumor, carcinomatosis and metastases to therapy and provide the basis for personalized treatment approach.Aim. To evaluate expression heterogeneity of ABC-transporter genes and chemosensitivity genes in gastric tumor, carcinomatosis and lymph node metastases.Materials and methods. Overall 41 patients with disseminated gastric cancer stage IV with carcinomatosis of peritoneum were included in the investigation. All patients underwent surgery according to Roux palliative gastrectomy. After surgery patients underwent chemotherapy depending on indications. RNA was isolated using RNeasy Plus mini kit (Qiagen, Germany). The expression level of ABC transporter genes (ABCB1, ABCC1, ABCC2, ABCC5, ABCG1, ABCG2) and chemosensitivity genes (BRCA1, RRM1, ERCC1, TOP1, TOP2α, TUBβ3, TYMS, GSTP1) was assessed by reverse transcription polymerase chain reaction (RT-PCR) in primary tumor, carcinomatosis and lymph node metastases.Results. The expression levels of the genes under study were shown to vary widely. For ABC transporter genes, ABCG1 (3.1 ± 1.1; max 32.0), ABCG2 (7.9 ± 2.3; max 54.1), ABCG2 (9.6 ± 3.8; max 101.0) were the most expressed genes in gastric tumor tissue, carcinomatosis and lymph node metastasis, respectively. Hyperexpression among chemosensitivity genes at all three sites was characteristic only of TOP2α (17.2 ± 6.0; max. 161.9; 10.8 ± 4.1; max. 105.1; 35.3 ± 0.8; max. 439.6, respectively). We found that TOP2α and BRCA1 gene expression levels were higher in lymph node metastasis compared with gastric tumor tissue and carcinomatosis (at p = 0.005 and p = 0.001). Whereas ABCC1 gene expression was statistically significantly higher in carcinomatosis (p = 0.03).Conclusion. Thus, a high level of expression heterogeneity is observed in gastric cancer, which affects the expression patterns of various genes in different localizations. The expression profile can be used to determine the level of heterogeneity and approach to personalized therapy tactics.Введение. Установлено, что метастатическим опухолям (в частности раку желудка) свойственна гетерогенность между первичными и метастатическими очагами. Такой тип гетерогенности является причиной низкой эффективности терапии, поэтому его следует учитывать при лечении первично-метастатических форм опухоли. В связи с этим сравнительный анализ экспрессии генов ABC-транспортеров и генов химиочувствительности позволит в определенной мере охарактеризовать резистентность и чувствительность первичной опухоли, канцероматоза и метастазов к терапии и дать основу для персонализированного подхода в лечении.Цель – оценка экспрессионной гетерогенности генов ABC-транспортеров и генов химиочувствительности в опухоли желудка, канцероматозе и метастазах в лимфатические узлы.Материалы и методы. В исследование включен 41 больной с диссеминированным раком желудка IV стадии с канцероматозом брюшины. Всем пациентам выполнена операция в объеме паллиативной гастрэктомии по Ру. После хирургического вмешательства в зависимости от показаний проводилась химиотерапия по схемам GemCap + С, FOLFIRI + С, XELIRI + C, PC. РНК выделяли с помощью набора RNeasy Plus mini Kit (Qiagen, Германия). Уровень экспрессии генов ABC-транспортеров (ABCB1, ABCC1, ABCC2, ABCC5, ABCG1, ABCG2) и генов химиочувствительности (BRCA1, RRM1, ERCC1, TOP1, TOP2α, TUBβ3, TYMS, GSTP1) в первичной опухоли, канцероматозе и метастазе в лимфатические узлы оценивали с помощью полимеразной цепной реакции с обратной транскрипцией (ОТ-ПЦР).Результаты. Показано, что уровень экспрессии исследуемых генов сильно варьирует. Из генов ABC-транспортеров наибольшее значение экспрессии для опухолевой ткани желудка, канцероматоза и метастаза в лимфатические узлы выявлено для ABCG1 (3,1 ± 1,1; max 32,0), ABCG2 (7,9 ± 2,3; max 54,1) и ABCG2 (9,6 ± 3,8; max 100,9) соответственно. Среди генов химиочувствительности гиперэкспрессия в этих тканях была характерна только для TOP2α (17,2 ± 5,9; max 161,9; 10,8 ± 4,1; max 105,1; 35,3 ± 0,8; max 439,6 соответственно). Установлено, что уровень экспрессии генов TOP2α и BRCA1 выше в метастазах в лимфатических узлах по сравнению с опухолевой тканью желудка и канцероматозом (p = 0,005 и p = 0,001 соответственно), тогда как экспрессия гена ABCC1 статистически значимо выше в канцероматозе (p = 0,03).Заключение. Таким образом, прослеживается высокий уровень экспрессионной гетерогенности при раке желудка, влияющей на паттерны экспрессии различных генов в разных локализациях. Точное определение профиля экспрессии исследуемых генов в первичной опухоли и метастазах позволит подобрать тактику лечения и схемы химиотерапии, которые с наибольшей вероятностью будут эффективны в каждом конкретном случае