Νεολαία και Ευρωπαϊκή Ένωση: Στάσεις, Προσδιοριστικοί Παράγοντες και Διαγενεακές Δυναμικές

Η παρούσα έκθεση πραγματεύεται τις ελληνικές στάσεις απέναντι στην ευρωπαϊκή ολοκλήρωση, με ιδιαίτερη έμφαση στην νεολαία. Από τη λεπτομερή ανάλυση πρωτογενών δεδομένων εντοπίστηκαν διακριτές διαφορές μεταξύ των γενεών, ανάμεσα στα άτομα ηλικίας κάτω και άνω των 35 ετών, τόσο στην εξέλιξη όσο και στους προσδιοριστικούς παράγοντες των στάσεων απέναντι στην Ευρωπαϊκή Ένωση (ΕΕ).Πρώτον, οι νέοι Έλληνες είναι γενικά πιο θετικά διακείμενοι απέναντι στην ΕΕ και πιο αισιόδοξοι για το μέλλον της σε σύγκριση με τις ομάδες μεγαλύτερης ηλικίας. Η εθνική (ελληνική) τους ταυτότητα δεν έρχεται σε αντίθεση με την ευρωπαϊκή : και οι δύο συνυπάρχουν και είναι αμφότερα σημαντικές για το πώς οι νέοι αξιολογούν τις μελλοντικές προοπτικές τους. Αντιθέτως, ο βαθμός στον οποίο οι νέοι είναι ευχαριστημένοι συνολικά από τη ζωή τους στην Ελλάδα επηρεάζει, σε μεγάλο βαθμό, τον τρόπο που βλέπουν την ΕΕ.Δεύτερον, οι νέοι Έλληνες έχουν απόλυτη συνείδηση ​​των ελλείψεων της ευρωπαϊκής ενοποίησης, όπως αυτές εκδηλώθηκαν στον χειρισμό της οικονομικής κρίσης. Συγκεκριμένα, θεωρούν ότι η ΕΕ έχει επωφεληθεί δυσανάλογα από την ελληνική ένταξη και όχι το αντίστροφο και είναι επικριτικοί για τον χειρισμό της κρίσης τόσο από τις ελληνικές κυβερνήσεις, όσο και από την ΕΕ.Τρίτον, μολαταύτα, οι νέοι στην Ελλάδα αρνούνται να συμμετάσχουν σε παιχνίδια απόδοσης ευθυνών για το ποιός έκανε τί και πότε. Αντιθέτως, επικεντρώνονται σε συγκεκριμένες δημόσιες πολιτικές, αναμένοντας ότι η ΕΕ θα συνεχίσει να βοηθά τη χώρα τους στο χειρισμό διεθνικών προβλημάτων, όπως η κλιματική αλλαγή και η μετανάστευση, και υποστηρίζοντας  τις μεταρρυθμίσεις της ελληνικής δημόσιας διοίκησης σύμφωνα με τις βέλτιστες πρακτικές της ΕΕ.Τέταρτον, οι νέοι Έλληνες απέχουν πολύ από το εγωιστικό στερεότυπο της μεγιστοποίησης της ατομικής ωφέλειας. Η στάση τους προς την ΕΕ επηρεάζεται έντονα από αυτό που θεωρούν ότι είναι συμφέρον της χώρας και όχι από τις προσωπικές τους οικονομικές συνθήκες. Οι νέοι επίσης αποδέχονται εμφατικά την προσωπική ευθύνη για την αντιμετώπιση συλλογικών προβλημάτων.Τέλος, οι στάσεις για την ευρωπαϊκή ενοποίηση επηρεάζονται από πολύ συγκεκριμένους στόχους πολιτικής. Η υποστήριξη στην ΕΕ συμβαδίζει με θετικές στάσεις απέναντι στην ιδιωτική επιχείρηση, την επιχειρηματικότητα, τις πολιτικές υπέρ της ανάπτυξης, τη μείωση της ανισότητας και του αυταρχισμού. Εκείνοι που βλέπουν την κρίση ως ευκαιρία υποστηρίζουν την περαιτέρω ολοκλήρωση, έχοντας επίγνωση του γεγονότος ότι οι κρίσεις μας επιτρέπουν να επανεκτιμήσουμε λάθη του παρελθόντος και να χαράξουμε νέες προοπτικές για το μέλλον. Το κεντρικό συμπέρασμα της έκθεσης, οπως προκύπτει από τη διεξοδική ανάλυση πρωτογενών δεδομένων, είναι ότι οι καλές πολιτικές, και όχι μόνον η καλή πολιτική, αποτελεί τον αποτελεσματικότερο τρόπο για να εδραιωθεί η ευρωπαϊκή προοπτική της Ελλάδας.Institutions, Decisions and Collective Behaviou

Ancestral Vascular Lumen Formation via Basal Cell Surfaces

The cardiovascular system of bilaterians developed from a common ancestor. However, no endothelial cells exist in invertebrates demonstrating that primitive cardiovascular tubes do not require this vertebrate-specific cell type in order to form. This raises the question of how cardiovascular tubes form in invertebrates? Here we discovered that in the invertebrate cephalochordate amphioxus, the basement membranes of endoderm and mesoderm line the lumen of the major vessels, namely aorta and heart. During amphioxus development a laminin-containing extracellular matrix (ECM) was found to fill the space between the basal cell surfaces of endoderm and mesoderm along their anterior-posterior (A-P) axes. Blood cells appear in this ECM-filled tubular space, coincident with the development of a vascular lumen. To get insight into the underlying cellular mechanism, we induced vessels in vitro with a cell polarity similar to the vessels of amphioxus. We show that basal cell surfaces can form a vascular lumen filled with ECM, and that phagocytotic blood cells can clear this luminal ECM to generate a patent vascular lumen. Therefore, our experiments suggest a mechanism of blood vessel formation via basal cell surfaces in amphioxus and possibly in other invertebrates that do not have any endothelial cells. In addition, a comparison between amphioxus and mouse shows that endothelial cells physically separate the basement membranes from the vascular lumen, suggesting that endothelial cells create cardiovascular tubes with a cell polarity of epithelial tubes in vertebrates and mammals

γCOP Is Required for Apical Protein Secretion and Epithelial Morphogenesis in Drosophila melanogaster

Background: There is increasing evidence that tissue-specific modifications of basic cellular functions play an important role in development and disease. To identify the functions of COPI coatomer-mediated membrane trafficking in Drosophila development, we were aiming to create loss-of-function mutations in the γCOP gene, which encodes a subunit of the COPI coatomer complex. Principal Findings: We found that γCOP is essential for the viability of the Drosophila embryo. In the absence of zygotic γCOP activity, embryos die late in embryogenesis and display pronounced defects in morphogenesis of the embryonic epidermis and of tracheal tubes. The coordinated cell rearrangements and cell shape changes during tracheal tube morphogenesis critically depend on apical secretion of certain proteins. Investigation of tracheal morphogenesis in γCOP loss-of-function mutants revealed that several key proteins required for tracheal morphogenesis are not properly secreted into the apical lumen. As a consequence, γCOP mutants show defects in cell rearrangements during branch elongation, in tube dilation, as well as in tube fusion. We present genetic evidence that a specific subset of the tracheal defects in γCOP mutants is due to the reduced secretion of the Zona Pellucida protein Piopio. Thus, we identified a critical target protein of COPI-dependent secretion in epithelial tube morphogenesis. Conclusions/Significance: These studies highlight the role of COPI coatomer-mediated vesicle trafficking in both general and tissue-specific secretion in a multicellular organism. Although COPI coatomer is generally required for protein secretion, we show that the phenotypic effect of γCOP mutations is surprisingly specific. Importantly, we attribute a distinct aspect of the γCOP phenotype to the effect on a specific key target protein

Trafficking through COPII Stabilises Cell Polarity and Drives Secretion during Drosophila Epidermal Differentiation

BACKGROUND: The differentiation of an extracellular matrix (ECM) at the apical side of epithelial cells implies massive polarised secretion and membrane trafficking. An epithelial cell is hence engaged in coordinating secretion and cell polarity for a correct and efficient ECM formation. PRINCIPAL FINDINGS: We are studying the molecular mechanisms that Drosophila tracheal and epidermal cells deploy to form their specific apical ECM during differentiation. In this work we demonstrate that the two genetically identified factors haunted and ghost are essential for polarity maintenance, membrane topology as well as for secretion of the tracheal luminal matrix and the cuticle. We show that they code for the Drosophila COPII vesicle-coating components Sec23 and Sec24, respectively, that organise vesicle transport from the ER to the Golgi apparatus. CONCLUSION: Taken together, epithelial differentiation during Drosophila embryogenesis is a concerted action of ECM formation, plasma membrane remodelling and maintenance of cell polarity that all three rely mainly, if not absolutely, on the canonical secretory pathway from the ER over the Golgi apparatus to the plasma membrane. Our results indicate that COPII vesicles constitute a central hub for these processes

A Systematic Screen for Tube Morphogenesis and Branching Genes in the Drosophila Tracheal System

Many signaling proteins and transcription factors that induce and pattern organs have been identified, but relatively few of the downstream effectors that execute morphogenesis programs. Because such morphogenesis genes may function in many organs and developmental processes, mutations in them are expected to be pleiotropic and hence ignored or discarded in most standard genetic screens. Here we describe a systematic screen designed to identify all Drosophila third chromosome genes (∼40% of the genome) that function in development of the tracheal system, a tubular respiratory organ that provides a paradigm for branching morphogenesis. To identify potentially pleiotropic morphogenesis genes, the screen included analysis of marked clones of homozygous mutant tracheal cells in heterozygous animals, plus a secondary screen to exclude mutations in general “house-keeping” genes. From a collection including more than 5,000 lethal mutations, we identified 133 mutations representing ∼70 or more genes that subdivide the tracheal terminal branching program into six genetically separable steps, a previously established cell specification step plus five major morphogenesis and maturation steps: branching, growth, tubulogenesis, gas-filling, and maintenance. Molecular identification of 14 of the 70 genes demonstrates that they include six previously known tracheal genes, each with a novel function revealed by clonal analysis, and two well-known growth suppressors that establish an integral role for cell growth control in branching morphogenesis. The rest are new tracheal genes that function in morphogenesis and maturation, many through cytoskeletal and secretory pathways. The results suggest systematic genetic screens that include clonal analysis can elucidate the full organogenesis program and that over 200 patterning and morphogenesis genes are required to build even a relatively simple organ such as the Drosophila tracheal system

Silkworm Coatomers and Their Role in Tube Expansion of Posterior Silkgland

Background: Coat protein complex I (COPI) vesicles, coated by seven coatomer subunits, are mainly responsible for Golgito-ER transport. Silkworm posterior silkgland (PSG), a highly differentiated secretory tissue, secretes fibroin for silk production, but many physiological processes in the PSG cells await further investigation. Methodology/Principal Findings: Here, to investigate the role of silkworm COPI, we cloned six silkworm COPI subunits (a,b,b9, d, e, and f-COP), determined their peak expression in day 2 in fifth-instar PSG, and visualized the localization of COPI, as a coat complex, with cis-Golgi. By dsRNA injection into silkworm larvae, we suppressed the expression of a-, b9- and c-COP, and demonstrated that COPI subunits were required for PSG tube expansion. Knockdown of a-COP disrupted the integrity of Golgi apparatus and led to a narrower glandular lumen of the PSG, suggesting that silkworm COPI is essential for PSG tube expansion. Conclusions/Significance: The initial characterization reveals the essential roles of silkworm COPI in PSG. Although silkworm COPI resembles the previously characterized coatomers in other organisms, some surprising findings require further investigation. Therefore, our results suggest the silkworm as a model for studying intracellular transport, and woul

Serrano (Sano) Functions with the Planar Cell Polarity Genes to Control Tracheal Tube Length

Epithelial tubes are the functional units of many organs, and proper tube geometry is crucial for organ function. Here, we characterize serrano (sano), a novel cytoplasmic protein that is apically enriched in several tube-forming epithelia in Drosophila, including the tracheal system. Loss of sano results in elongated tracheae, whereas Sano overexpression causes shortened tracheae with reduced apical boundaries. Sano overexpression during larval and pupal stages causes planar cell polarity (PCP) defects in several adult tissues. In Sano-overexpressing pupal wing cells, core PCP proteins are mislocalized and prehairs are misoriented; sano loss or overexpression in the eye disrupts ommatidial polarity and rotation. Importantly, Sano binds the PCP regulator Dishevelled (Dsh), and loss or ectopic expression of many known PCP proteins in the trachea gives rise to similar defects observed with loss or gain of sano, revealing a previously unrecognized role for PCP pathway components in tube size control

Duox, Flotillin-2, and Src42A Are Required to Activate or Delimit the Spread of the Transcriptional Response to Epidermal Wounds in Drosophila

The epidermis is the largest organ of the body for most animals, and the first line of defense against invading pathogens. A breach in the epidermal cell layer triggers a variety of localized responses that in favorable circumstances result in the repair of the wound. Many cellular and genetic responses must be limited to epidermal cells that are close to wounds, but how this is regulated is still poorly understood. The order and hierarchy of epidermal wound signaling factors are also still obscure. The Drosophila embryonic epidermis provides an excellent system to study genes that regulate wound healing processes. We have developed a variety of fluorescent reporters that provide a visible readout of wound-dependent transcriptional activation near epidermal wound sites. A large screen for mutants that alter the activity of these wound reporters has identified seven new genes required to activate or delimit wound-induced transcriptional responses to a narrow zone of cells surrounding wound sites. Among the genes required to delimit the spread of wound responses are Drosophila Flotillin-2 and Src42A, both of which are transcriptionally activated around wound sites. Flotillin-2 and constitutively active Src42A are also sufficient, when overexpressed at high levels, to inhibit wound-induced transcription in epidermal cells. One gene required to activate epidermal wound reporters encodes Dual oxidase, an enzyme that produces hydrogen peroxide. We also find that four biochemical treatments (a serine protease, a Src kinase inhibitor, methyl-ß-cyclodextrin, and hydrogen peroxide) are sufficient to globally activate epidermal wound response genes in Drosophila embryos. We explore the epistatic relationships among the factors that induce or delimit the spread of epidermal wound signals. Our results define new genetic functions that interact to instruct only a limited number of cells around puncture wounds to mount a transcriptional response, mediating local repair and regeneration