Boops boops as a bioindicator of microplastic pollution along the Spanish Catalan coast

Microplastic pollution is a growing cause of concern for the marine environment, particularly in the Mediterranean Sea, which is considered to be one of the most polluted seas worldwide. In this study, the gastrointestinal tracts of 102 bogues (Boops boops), sampled from three areas off the Catalan coast (Spain) subject to different degrees of industrialization, were analysed to assess microplastic ingestion and thus estimate local levels of microplastic pollution. Microplastics were detected in 46% of samples analysed. As expected, the abundance and frequency of occurrence of ingested microplastics were higher off the most anthropized area of Barcelona. The majority of ingested microplastics were blue fragments ranging 0.1-0.5 mm, and the most common polymer type was polypropylene. The results of this study indicate the area off Barcelona as a possible area of concentration for microplastics, further supporting the use of B. boops as a bioindicator to assess microplastic pollution

A MSFD complementary approach for the assessment of pressures, knowledge and data gaps in Southern European Seas : the PERSEUS experience

PERSEUS project aims to identify the most relevant pressures exerted on the ecosystems of the Southern European Seas (SES), highlighting knowledge and data gaps that endanger the achievement of SES Good Environmental Status (GES) as mandated by the Marine Strategy Framework Directive (MSFD). A complementary approach has been adopted, by a meta-analysis of existing literature on pressure/impact/knowledge gaps summarized in tables related to the MSFD descriptors, discriminating open waters from coastal areas. A comparative assessment of the Initial Assessments (IAs) for five SES countries has been also independently performed. The comparison between meta-analysis results and IAs shows similarities for coastal areas only. Major knowledge gaps have been detected for the biodiversity, marine food web, marine litter and underwater noise descriptors. The meta-analysis also allowed the identification of additional research themes targeting research topics that are requested to the achievement of GES. 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license.peer-reviewe

More threats for the Mediterranean monk seal: presence of microplastics in their diet

MICRO 2020 International Conference, Fate of Impact of Microplastics in Marine Ecosystems, 23-27 November 2020For the very first time microplastics were systematically analysed in feaces of the endangered Mediterranean monk seal (Monachus monachus) in Greece, as a top marine predator. This study was carried out in a joint project by the Hellenic Centre for Marine Research (HCMR) and the Greek NGO Archipelagos ¿ environment and development within the framework of the project ¿PLASTIC BUSTERS MPA¿. Twelve samples were collected in monk seal caves along the southwestern coasts of Zakynthos island, Ionian Sea, Greece, according to a special protocol elaborated for this purpose. The set of samples was analysed at the HCMR. A total of 208 particles were detected 42 of which (19.2%) were identified as organic material and were discarded from the analysis. The length of anthropogenic particles ranged from 45¿m to 28,763¿m, though 75% of the particles ranged from 100 to 3000¿m. Interestingly, 34.2% of the particles were smaller than 1,000¿m and only 10.9% were larger than 5,000¿m. Most of the particles identified were filaments (n=140, 84,3%), followed by fragments (n=24, 14.5%). Transparent particles were the most abundant ones (39.8%), followed by blue ones (39.2%). Red particles occurred in 7.8%, while green and black occurred in 6.0% and 3.0% respectively. Most of the transparent and blue particles were filaments (92.5% and 89.2% respectively). The majority of the particles identified were polyamides (73.8%), followed by polycarbonate (14.8%), and polypropylene (6.6%). Because all faeces samples analysed contained microplastics the exposure of monk seals to this pollutant might be a cause of concern. Further investigations are necessary for drawing firm conclusionsN

Exploring microplastic pollution in a Mediterranean river: The role of introduced species as bioindicators

Studies of plastic contamination in freshwater ecosystems and their biota remain scarce, despite the fact that the vast majority of plastic waste initially passes through lotic ecosystems. Biomonitoring provides valuable information regarding plastic pollution and microplastic threats to biota and human health. The aim of this study was to explore the potential use of a non-indigenous fish species as a bioindicator of microplastic pollution in an Eastern Mediterranean River. Our study area is located in a heavily modified and vastly impacted urban river which flows through the largest part of the Metropolitan area of Athens, Greece. We used an introduced chub species (Squalius vardarensis) to assess microplastic ingestion in the river. The results indicated moderate occurrence and abundance of microplastics in the fish gastrointestinal tracts; one-third of specimens (35%) contained microplastics, although the average number of microplastics per specimen was relatively low (1.7 ± 0.2). Overall, the abundance of microplastics in the water confirmed the moderate level of microplastics contamination in our study area. The major polymer types of microplastics identified by FT-IR analysis were: polyethylene (PE), polyvinyl alcohol (PVA) and polypropylene (PP); reflecting the fragmentation of larger litter from industrial packaging and/or household goods. Surface runoff of the urban environment, via motorways and major road networks, could be the contributing factor to the reported microplastics. Our results suggest that generalist's non-indigenous species such as chubs could be used as bioindicators of microplastics in inland waters. Introduced fishes can be a feasible, nondestructive, and cost-effective option for the assessment of microplastics in freshwater ecosystems, while freshwater chubs' high abundance and omnipresence in European rivers further serve this scope. However, it is worth noting that the suitability of any particular species as a bioindicator of microplastics may depend on a variety of factors, including their feeding behavior, habitat, and exposure to microplastics in their environment

Assessment of organophosphate flame retardants in Mediterranean Boops boops and their relationship to anthropization levels and microplastic ingestion

Plastic litter pollution is increasing in the seas and oceans worldwide, raising concern on the potential effects of plasticizer additives on marine fauna. In this study, muscle samples of 30 bogues (Boops boops; Linneaus, 1758) from the North Western Mediterranean Sea were analysed to assess the concentrations of 19 organophosphate flame retardant (OPFR) compounds and to inspect any relationship with microplastic ingestion and relative levels of anthropization. Out of the 19 OPFRs analysed, 6 compounds were detected, being tri-n-butyl phosphate (TNBP), 2-ethylhexyldiphenyl phosphate (EHDPP) and triphenylphosphine oxide (TPPO) the most abundant. As expected, OPFR concentrations were higher in samples collected off the most anthropized area of the city of Barcelona than in those from the Cap de Creus Marine Protected Area, while no significant correlation was detected between OPFR concentrations and microplastic ingestion. The results of this manuscript provide a first evidence of OPFR presence in the muscle of the bogue and identify the coastal area off Barcelona as a possible concentration area for contaminants, further supporting the use of the bogue as an indicator species of plastic pollution in the Mediterranean Sea.This study was supported by the Spanish Ministry of Science and Innovation (Project CEX2018-000794-S) and by the Generalitat de Catalunya (Consolidated Research Group Water and Soil Quality Unit 2017 SGR 1404). The authors are grateful to the Confradia de pescadors de Barcelona and the Confradia de pescadors de Roses, which provided the bogues samples. OGG’s work was funded through an FPU Ph.D. scholarship granted by the Spanish Government. Constructive feedback from two anonymous reviewers contributed to improve the manuscript.Peer reviewe

Report on the approaches implemented in the Mediterranean countries for GES descriptor 8. Proposition of a road map for a better harmonization

Based on the data gap analysis performed in the first step of MEDCIS, the Medcis team on contaminant proposed a road map associated with a time-schedule in order to improve harmonization in Mediterranean region for 2024 GES assessment. Partner countries shared information on monitored substances and biological effects, thresholds used, matrix/matrices used for the monitoring of each substance/family of substances, the limit of quantification (LQ) of each chemical measurement, and the proportion of censored dat

Interlaboratory comparison of microplastic extraction methods from marine biota tissues: A harmonization exercise of the Plastic Busters MPAs project

In the framework of the Plastic Busters MPAs project, a harmonization exercise on two methods of microplastic extraction from biological samples i.e. 15% H2O2 digestion and 10% KOH digestion was carried out. The two methods were tested in four laboratories on fish gastrointestinal tracts and mussel tissues spiked with polyethylene, polypropylene and polyethylene terephthalate. The recovery percentage of microplastics for each method, species and polymer tested were overall similar among laboratories, and interlaboratory coefficient of variation was less than 11% for the majority of samples. Microplastic recovery rates for the two methods were similar for each sample tested, but overall mean interlaboratory recovery rate using KOH (96.67%) was higher than H2O2 (88.75%). Results validate the use of both methods for extracting microplastics from biota tissues. However, when comparing the two methods in terms of microplastic recovery rate, time consumed, technical difficulties and cost, digestion with 10% KOH is considered optimal.En prensa2,35

Using Boops boops (osteichthyes) to assess microplastic ingestion in the Mediterranean Sea

This study assesses microplastic ingestion in Boops boops at different geographical areas in the Mediterranean Sea. A total of 884 fish were caught at 20 coastal sites in Spain, France, Italy and Greece and analyzed using a common methodological protocol. Microplastics were found in 46.8% of the sampled fish, with an average number of items per individual of 1.17 +/- 0.07. Filaments were the predominant shape type, while polyethylene and polypropylene were indicated by FTIR as the most common polymer types of ingested microplastics. The frequency of occurrence, as well as the abundance and proportion of types (size, shape, color and polymer) of ingested microplastics, varied among geographical areas. The spatial heterogeneity of the abundance of ingested microplastics was mainly related to the degree of coastal anthropogenic pressure at the sampling sites. Our findings further support the suitability of B. boops as bioindicator of microplastic pollution in the Mediterranean Sea

Bioindicators for monitoring marine litter ingestion and its impacts on Mediterranean biodiversity

The Mediterranean Sea has been described as one of the most affected areas by marine litter in the world. Although effects on organisms from marine plastic litter ingestion have been investigated in several oceanic areas, there is still a lack of information from the Mediterranean Sea. The main objectives of this paper are to review current knowledge on the impact of marine litter on Mediterranean biodiversity, to define selection criteria for choosing marine organisms suitable for use as bioindicator species, and to propose a methodological approach to assessing the harm related to marine litter ingestion in several Mediterranean habitats and sub-regions. A new integrated monitoring tool that would provide the information necessary to design and implement future mitigation actions in the Mediterranean basin is proposed. According to bibliographic research and statistical analysis on current knowledge of marine litter ingestion, the area of the Mediterranean most studied, in terms of number of species and papers in the Mediterranean Sea is the western sub-area as well as demersal (32.9%) and pelagic (27.7%) amongst habitats. Applying ecological and biological criteria to the most threatened species obtained by statistical analysis, bioindicator species for different habitats and monitoring scale were selected. A threefold approach, simultaneously measuring the presence and effects of plastic, can provide the actual harm and sub-lethal effects to organisms caused by marine litter ingestion. The research revealed gaps in knowledge, and this paper suggests measures to close the gap. This and the selection of appropriate bioindicator species would represent a step forward for marine litter risk assessment, and the implementation of future actions and mitigation measures for specific Mediterranean areas, habitats and species affected by marine litter ingestionVersión del editor3,74