Lucky Spectroscopy, an equivalent technique to Lucky Imaging. Spatially resolved spectroscopy of massive close visual binaries using the William Herschel Telescope

CONTEXT: Many massive stars have nearby companions whose presence hamper their characterization through spectroscopy. AIMS: We want to obtain spatially resolved spectroscopy of close massive visual binaries to derive their spectral types. METHODS: We obtain a large number of short long-slit spectroscopic exposures of five close binaries under good seeing conditions, select those with the best characteristics, extract the spectra using multiple-profile fitting, and combine the results to derive spatially separated spectra. RESULTS: We demonstrate the usefulness of Lucky Spectroscopy by presenting the spatially resolved spectra of the components of each system, in two cases with separations of only ~0.3". Those are delta Ori Aa+Ab (resolved in the optical for the first time) and sigma Ori AaAb+B (first time ever resolved). We also spatially resolve 15 Mon AaAb+B, zeta Ori AaAb+B (both previously resolved with GOSSS, the Galactic O-Star Spectroscopic Survey), and eta Ori AaAb+B, a system with two spectroscopic B+B binaries and a fifth visual component. The systems have in common that they are composed of an inner pair of slow rotators orbited by one or more fast rotators, a characteristic that could have consequences for the theories of massive star formation.Comment: Accepted for publication in A&A, 7 page

Influence of Baseline Fluctuation Cancellation on Automatic Measurement of Motor Unit Action Potential Duration

The aim of this work is to analyze the influence of a method for baseline fluctuation (BLF) cancellation for electromyographic (EMG) signals on automatic methods for measurement of the motor unit action potential (MUAP) duration. These methods include four conventional automatic methods (CAMs) and a recently published wavelet transform method (WTM). A set of 182 MUAPs from 170 EMG recordings were studied. The CAMs and the WTM were applied to the MUAPs before and after applying BLF cancellation to the recordings. A gold standard of duration marker positions (GSP) ws manually established. The accuracy of each algorithm was estimated as the dfference between its positions and the GSP. Accuracies were compared for the 5 methods and for each method before and after BLF cancellation. A significant difference between accuracy pre- and post-BLF removal was found in two CAMs; markers were closer to the GSP after BLF removal. For all MUAPs, the differences between WTM markers and the GSP were the smallest, and significant differences were not found for the WTM before and after BLF cancellation. The management of BLF is an important issue in EMG signal processing and BLF removal must be considered in extraction and analyse of MUAP waveforms. The BLF removal method improved the performance of two CAMs for MUAP duration measurement. The WTM was the most accurate and was not affected by BLF.

Mapping nanomechanical properties of live cells using multi-harmonic atomic force microscopy

The nanomechanical properties of living cells, such as their surface elastic response and adhesion, have important roles in cellular processes such as morphogenesis(1), mechano-transduction(2), focal adhesion(3), motility(4,5), metastasis(6) and drug delivery(7-10). Techniques based on quasi-static atomic force microscopy techniques(11-17) can map these properties, but they lack the spatial and temporal resolution that is needed to observe many of the relevant details. Here, we present a dynamic atomic force microscopy(18-28) method to map quantitatively the nanomechanical properties of live cells with a throughput (measured in pixels/minute) that is similar to 10-1,000 times higher than that achieved with quasi- static atomic force microscopy techniques. The local properties of a cell are derived from the 0th, 1st and 2nd harmonic components of the Fourier spectrum of the AFM cantilevers interacting with the cell surface. Local stiffness, stiffness gradient and the viscoelastic dissipation of live Escherichia coli bacteria, rat fibroblasts and human red blood cells were all mapped in buffer solutions. Our method is compatible with commercial atomic force microscopes and could be used to analyse mechanical changes in tumours, cells and biofilm formation with sub-10 nm detail

Proyecto de biblioteca virtual sanitaria en un portal de cooperación para países en desarrollo

Ponencias de la Segunda Conferencia internacional sobre brecha digital e inclusión social, celebrada del 28 al 30 de octubre de 2009 en la Universidad Carlos III de MadridSe presenta el proyecto de diseño y desarrollo de una biblioteca virtual destinada a satisfacer las necesidades de información científica y de apoyo asistencial de aquellos sanitarios que se encuentran desarrollando tareas humanitarias en la cooperación internacional fuera de España. Impulsada por la Dirección General de Salud Pública de la Generalitat Valenciana, esta Biblioteca forma parte de un portal o plataforma virtual denominado AYUDSAN. La metodología seguida para realizar el proyecto de biblioteca virtual ha sido la de diseñar una página Web, en la que tras estudiar las fuentes de información más importante en materia biosanitaria, se ha realizado una amplia selección de recursos de acceso abierto y de carácter gratuito, elaborado un formulario Web y facilitado una dirección electrónica para que los usuarios puedan realizar sus consultas a través de un servicio de referencia virtual

A computerized analysis of the entire retinal ganglion cell population and its spatial distribution in adult rats

AbstractIn adult albino (SD) and pigmented (PVG) rats the entire population of retinal ganglion cells (RGCs) was quantified and their spatial distribution analyzed using a computerized technique. RGCs were back-labelled from the optic nerves (ON) or the superior colliculi (SCi) with Fluorogold (FG). Numbers of RGCs labelled from the ON [SD: 82,818±3,949, n=27; PVG: 89,241±3,576, n=6) were comparable to those labelled from the SCi [SD: 81,486±4,340, n=37; PVG: 87,229±3,199; n=59]. Detailed methodology to provide cell density information at small scales demonstrated the presence of a horizontal region in the dorsal retina with highest densities, resembling a visual streak

Adaptation and Testing of the Factorial Structure of the Physical Education Grit Scale for Use in Secondary Education in Spain

The challenges faced by students during Physical Education classes embrace both physical and academic aspects. Therefore, each individual possesses a series of internal psychological mechanisms, such as Grit, which allow them to adapt and overcome the vicissitudes. However, there are no scales that assess Grit in Span. Thus, the aim of the present study is to test the factor structure of the Physical Education Grit Scale in Span of Physical Education classes. For this purpose, 857 secondary school students took part in the present study. In order to test the factor structure, a confirmatory factor analysis, an exploratory factor analysis, an analysis of the reliability of the questionnaire and an analysis of temporal stability were carried out. The results showed that the factor structure consisted of two factors and four sub-factors (¿2/df = 2.17 (p = 0.001); CFI = 0.96; TLI = 0.96; IFI = 0.96; RMSEA = 0.051; SRMR = 0.037). In addition, the reliability and temporal stability analyses showed acceptable indices. Based on these results, evidence of reliability and validity of the Physical Education Grit Scale in Span of Physical Education is provided

A search for Galactic runaway stars using Gaia Data Release 1 and Hipparcos proper motions

CONTEXT.The first Gaia Data Release (DR1) significantly improved the previously available proper motions for the majority of the Tycho-2 stars. AIMS. We want to detect runaway stars using Gaia DR1 proper motions and compare our results with previous searches. METHODS. Runaway O stars and BA supergiants are detected using a 2-D proper-motion method. The sample is selected using Simbad, spectra from our GOSSS project, literature spectral types, and photometry processed using CHORIZOS. RESULTS. We detect 76 runaway stars, 17 (possibly 19) of them with no prior identification as such, with an estimated detection rate of approximately one half of the real runaway fraction. An age effect appears to be present, with objects of spectral subtype B1 and later having travelled for longer distances than runaways of earlier subtypes. We also tentatively propose that the fraction of runaways is lower among BA supergiants that among O stars but further studies using future Gaia data releases are needed to confirm this. The frequency of fast rotators is high among runaway O stars, which indicates that a significant fraction of them (and possibly a majority) is produced in supernova explosions.Comment: Accepted for publication in A&

A physiological marker for quantifying differential reproductive investment between the sexes in Yellow-legged gulls (Larus michahellis)

Asymmetry between males and females in the energy they invest initially in reproduction has resulted in the evolution of differing reproductive strategies (caring females vs. competitive males). However, parental care in many birds is shared by both sexes suggesting that male energy expenditure in agonistic behaviors and courtship feeding might compensate female costs of clutch production. Here, we tested the hypothesis that initial investment in reproduction by both sexes in the Yellow-legged Gull (Larus michahellis), a species with biparental care, is similar from a physiological perspective. In this income breeder, female and male reproductive investment during early breeding can be ultimately related to muscular activity (local foraging effort required for clutch production in females and courtship feeding and agonistic behaviors in the case of males). Thus, we evaluated sex-specific patterns of creatine kinase (CK, IU/L) levels in plasma, an indicator of physical effort associated with muscular activity dependent behaviors, through incubation as a reflection of the physiological response of both sexes to the reproductive investment they made up to clutch completion. Raw levels of CK were related to plasma levels of total proteins (TP, g/dL) to account for the differential physiological state of individuals when sampled (i.e. differential dehydratation). Female costs of clutch production were associated with post-laying levels of CK/TP. We grouped females according to their relative investment in clutch production: 17.3% of field metabolic rate; which showed increasing values of CK/TP (24.6, 53.1 and 66.0 IU/g, respectively). Moreover, we found similar CK/TP trends throughout incubation for both sexes (CK/TP = 50.2- [3.3 x days from laying]) suggesting similar physiological responses to reproductive effort and, therefore, analogous sex-specific initial investment. Thus, male investment in agonistic behaviors and courtship feeding apparently equaled female investment in clutch production. The use of CK measurements is revealed as a useful approach to investigating overall investment in reproduction for both sexes, providing further insights into our comprehension of reproductive strategies in seabirds

Soil microbes and community coalescence

Community coalescence is a recently introduced term describing the interaction of entire communities and their environments. We here explicitly place the concept of community coalescence in a soil microbial context, exploring intrinsic and extrinsic drivers of such coalescence events. Examples of intrinsic events include the action of earthworms and the dynamics of soil aggregates, while extrinsic events are exemplified by tillage, flooding, litterfall, outplanting, and the addition of materials containing microbial communities. Aspects of global change may alter the frequency or severity of coalescence events. We highlight functional consequences of community coalescence in soil, and suggest ways to experimentally tackle this phenomenon. Soil ecology as a whole stands to benefit from conceptualizing soil biodiversity in terms of dynamic coalescent microbial assemblages