Particle sizing in the process industry using Hertz-Zener impact theory and acoustic emission spectra

The cost of implementing real-time monitoring and control of industrial processes is a significant barrier for many companies. Acoustic techniques provide complementary information to optical spectroscopic sensors and have a number of advantages: they are relatively inexpensive, can be applied non-invasively, are non-destructive, multi-point measurements are possible, opaque samples can be analysed in containers that are made from opaque materials (e.g. steel or concrete) and the analysis can be conducted in real-time. In this paper a new theoretical model is proposed which describes the transport of particles in a stirred reactor, their collision with the reactor walls, the subsequent vibrations which are then transmitted through the vessel walls, and their detection by an ultrasonic transducer. The particle-wall impact is modelled using Hertz-Zener impact theory. Experimental data is then used in conjunction with this (forward) model to form an inverse problem for the particle size distribution using a least squares cost function. Application of an integral smoothing operator to the power spectra greatly enhances the accuracy and robustness of the approach. One advantage of this new approach is that since it operates in the frequency domain, it can cope with the industrially relevant case of many particle-wall collisions. The technique will be illustrated using data from a set of controlled experiments. In the first instance a set of simplified experiments involving single particles being dropped in air onto a substrate are utilised. The second set of experiments involves particles in a carrier fluid being stirred in a reactor vessel. In each case the approach is able to successfully recover the associated particle size

Source areas evolution in the Neogene Agost Basin (Betic Cordillera): implications for regional reconstructions

Sedimentary and mineralogical analyses were performed in the Neogene Agost Basin (External Domain, Betic Cordillera) to reconstruct relationships between tectonics and sedimentation, and source areas evolution over time. The sedimentary analysis allowed defining two sedimentary sequences: (1) Lower Stratigraphic Unit, Serravallian p.p. and (2) Upper Stratigraphic Unit, post Lower Tortonian (Upper Miocene p.p.) separated by an angular unconformity. They consist of marine (lithofacies L-1 to L-3) and continental (lithofacies L-4 to L-8) deposits, respectively. The analysis of mineralogical assemblages and some XRD parameters of the sedimentary sequences and older formations allowed recognizing a sedimentary evolution controlled by the activation of different source areasover time. In particular, the Ill+Kln±Sme+Chl claymineral association characterizes the supply from Triassic formations; the Ill+Kln+Sme association from Albian formations; the Sme+Ill±Kln+(I-S) and Sme+Ill±Kln associations from Upper Cretaceous p.p. formations; and the Sme+Ill±Kln+(I-S) association from Paleogene formations, testifying a tectonic mobility of the basin margins differentiated over time. This reconstruction leads to propose detailed relationships between types of deposits and provenance and not a classic ?unroofing?, as follows: (i) the lithofacies L-1 (lithofacies L-2 and L-3 were not analysed) is characterized by the Ill+Kln+Sme mineralogical association indicating an origin from the Albian formations; (ii) the lithofacies L-4 shows a mixture of Ill+Kln+Sme and Sme+Ill+Kln associations sourced from the Albian and Upper Cretaceous formations; (iii) the lithofacies L-5 is characterized by the Sme+Ill±Kln+(I-S) association indicating a provenance from the Upper Cretaceous and Paleogene formations, and (iv) the lithofacies L-6 to L-8 are characterized by the Ill+Kln±Sme+Chl association indicating a supply mainly from Triassic deposits. The evolutionary sedimentary model reconstructed for the Agost Basin, which improves a previous contribution about the same area, has been correlated with those reported in other intramontane Neogene basins in the Betic-Rifian Arc studied with similar resolution, this obtaining useful information for regional reconstructions.Research supported by: Research Project CGL2016-75679-P, Spanish Ministry of Education and Science; Research Groups and Projects of the Generalitat Valenciana, Alicante University (CTMAIGA); Research Group RNM 146, Junta de Andalucía; Grants from University of Urbino Carlo Bo, responsible M. Tramontana

Internal controls for quantitative polymerase chain reaction of swine mammary glands during pregnancy and lactation.

High-throughput microarray analysis is an efficient means of obtaining a genome-wide view of transcript profiles across physiological states. However, quantitative PCR (qPCR) remains the chosen method for high-precision mRNA abundance analysis. Essential for reliability of qPCR data is normalization using appropriate internal control genes (ICG), which is now, more than ever before, a fundamental step for accurate gene expression profiling. We mined mammary tissue microarray data on >13,000 genes at -34, -14, 0, 7, 14, 21, and 28 d relative to parturition in 27 crossbred primiparous gilts to identify suitable ICG. Initial analysis revealed TBK1, PCSK2, PTBP1, API5, VAPB, QTRT1, TRIM41, TMEM24, PPP2R5B, and AP1S1 as the most stable genes (sample/reference = 1 +/- 0.2). We also included 9 genes previously identified as ICG in bovine mammary tissue. Gene network analysis of the 19 genes identified AP1S1, API5, MTG1, VAPB, TRIM41, MRPL39, and RPS15A as having no known co-regulation. In addition, UXT and ACTB were added to this list, and mRNA abundance of these 9 genes was measured by qPCR. Expression of all 9 of these genes was decreased markedly during lactation. In a previous study with bovine mammary tissue, mRNA of stably expressed genes decreased during lactation due to a dilution effect brought about by large increases in expression of highly abundant genes. To verify this effect, highly abundant mammary genes such as CSN1S2, SCD, FABP3, and LTF were evaluated by qPCR. The tested ICG had a negative correlation with these genes, demonstrating a dilution effect in the porcine mammary tissue. Gene stability analysis identified API5, VABP, and MRPL39 as the most stable ICG in porcine mammary tissue and indicated that the use of those 3 genes was most appropriate for calculating a normalization factor. Overall, results underscore the importance of proper validation of internal controls for qPCR and highlight the limitations of using absence of time effects as the criteria for selection of appropriate ICG. Further, we showed that use of the same ICG from one organism might not be suitable for qPCR normalization in other species

Single Nucleotide Polymorphisms in collagen genes and association with skin quality trait

AbstractLivestock skin is largely employed in the manufacturing of clothing and shoes, sector in which Italy is a world leader. To sustain Italian products against foreign competition in the globalization era particular attention is to be focus on product quality. Here we investigate the association of SNP mutations in genes coding for collagen proteins present in animal skin with a number of phisico-chemical parameters influencing skin quality for the tanning industry.Skin and blood were sampled from 73 Italian Friesian and Italian Brown bovines and from 43 Bergamasca and Sarda ovines, classified by sex and age. Skins were characterised for a set of chemico-physical parameters (thickness, density, humidity, protein content, ashes, lipid content, hydrossi-proline and DNA content).Regions of the collagen type I, III and IV were screened for SNP discovery in the two species by sequencing a set of reference animals. In bovine 15 polymorphisms were identified: (2 in collagen type I, 9 in collagen type III alp..

Intercomparison of methods to estimate gross primary production based on CO2 and COS flux measurements

Separating the components of ecosystem-scale carbon exchange is crucial in order to develop better models and future predictions of the terrestrial carbon cycle. However, there are several uncertainties and unknowns related to current photosynthesis estimates. In this study, we evaluate four different methods for estimating photosynthesis at a boreal forest at the ecosystem scale, of which two are based on carbon dioxide (CO2) flux measurements and two on carbonyl sulfide (COS) flux measurements. The CO2-based methods use traditional flux partitioning and artificial neural networks to separate the net CO2 flux into respiration and photosynthesis. The COS-based methods make use of a unique 5-year COS flux data set and involve two different approaches to determine the leaf-scale relative uptake ratio of COS and CO2 (LRU), of which one (LRUCAP) was developed in this study. LRUCAP was based on a previously tested stomatal optimization theory (CAP), while LRUPAR was based on an empirical relation to measured radiation. For the measurement period 2013-2017, the artificial neural network method gave a GPP estimate very close to that of traditional flux partitioning at all timescales. On average, the COS-based methods gave higher GPP estimates than the CO2-based estimates on daily (23 % and 7 % higher, using LRUPAR and LRUCAP, respectively) and monthly scales (20 % and 3 % higher), as well as a higher cumulative sum over 3 months in all years (on average 25 % and 3 % higher). LRUCAP was higher than LRU estimated from chamber measurements at high radiation, leading to underestimation of midday GPP relative to other GPP methods. In general, however, use of LRUCAP gave closer agreement with CO2-based estimates of GPP than use of LRUPAR. When extended to other sites, LRUCAP may be more robust than LRUPAR because it is based on a physiological model whose parameters can be estimated from simple measurements or obtained from the literature. In contrast, the empirical radiation relation in LRUPAR may be more site-specific. However, this requires further testing at other measurement sites.Peer reviewe

Tectono-Sedimentary Cenozoic Evolution of the El Habt and Ouezzane Tectonic Units (External Rif, Morocco)

An interdisciplinary study based on lithostratigraphic, biostratigraphic, petrographic and mineralogical analyses has been performed in order to establish the Cenozoic tectono-sedimentary evolution of the El Habt and Ouezzane Tectonic Units (External Intrarif Subzone, External Rif, Morocco). The reconstructed record allowed identification of the depositional architecture and related sedimentary processes of the considered units. The Cenozoic successions were biochronologically defined allowing, at the same time, identification of unconformities and associated stratigraphic gaps. The presence of five unconformities allowed for the definition of the main stratigraphic units arranged in a regressive trend: (1) lower Paleocene interval (Danian p.p.) assigned to a deep basin; (2) Eocene interval (lower Ypresian-lower Bartonian p.p.) from a deep basin to an external carbonate-siliceous platform; (3) lower Rupelian-upper Chattian p.p. interval deposited on unstable slope with turbidite channels passing upward to an external siliciclastic platform; (4) Burdigalian p.p. interval from a slope; (5) Langhian-Serravallian p.p. interval from slope to external platform realms. The petrography of the arenites and calcarenites allowed for the identification of the supplies derived from erosion of a recycled orogen (transitional and quartzose subtypes). The clay-mineralogy analysis indicates an unroofing (first erosion of Cretaceous terrains followed by upper Jurassic rocks) always accomplished by erosion of Cenozoic terrains. Several tectofacies checked in some stratigraphic intervals seem to indicate the beginning of deformation of the basement generating gentle folds and first activation of blind thrusts, mainly during the Paleogene. A preorogenic tectonic framework is considered as responseto the generalized tectonic inversion (from extension to compression) as frequently registered in the central-western peri-Mediterranean areas. The large volumes of reworked terrigeneous supply during the latest Oligocene-Miocene p.p. indicates the beginningsof the synorogenic sedimentation (foredeep stage of the basins) controlled by active tectonics.Research Project CGL2016-75679-P (Spanish Ministry of Education and Science); Research Groups, Projects of the Generalitat Valenciana, Alicante University (CTMA-IGA); Grants from the University of Urbino Carlo Bo (M. Tramontana)

First full-beam PET acquisitions in proton therapy with a modular dual-head dedicated system

During particle therapy irradiation, positron emitters with half-lives ranging from 2 to 20 min are generated from nuclear processes. The half-lives are such that it is possible either to detect the positron signal in the treatment room using an in-beam positron emission tomography (PET) system, right after the irradiation, or to quickly transfer the patient to a close PET/CT scanner. Since the activity distribution is spatially correlated with the dose, it is possible to use PET imaging as an indirect method to assure the quality of the dose delivery. In this work, we present a new dedicated PET system able to operate in-beam. The PET apparatus consists in two 10 cm × 10 cm detector heads. Each detector is composed of four scintillating matrices of 23 × 23 LYSO crystals. The crystal size is 1.9 mm × 1.9 mm × 16 mm. Each scintillation matrix is read out independently with a modularized acquisition system. The distance between the two opposing detector heads was set to 20 cm. The system has very low dead time per detector area and a 3 ns coincidence window, which is capable to sustain high single count rates and to keep the random counts relatively low. This allows a new full-beam monitoring modality that includes data acquisition also while the beam is on. The PET system was tested during the irradiation at the CATANA (INFN, Catania, Italy) cyclotron-based proton therapy facility. Four acquisitions with different doses and dose rates were analysed. In all cases the random to total coincidences ratio was equal or less than 25%. For each measurement we estimated the accuracy and precision of the activity range on a set of voxel lines within an irradiated PMMA phantom. Results show that the inclusion of data acquired during the irradiation, referred to as beam-on data, improves both the precision and accuracy of the range measurement with respect to data acquired only after irradiation. Beam-on data alone are enough to give precisions better than 1 mm when at least 5 Gy are delivered

Miocene tectono-sedimentary evolution of the eastern external Betic Cordillera (Spain)

An interdisciplinary study of Miocene successions in the eastern External Betic Zone (South Iberian Margin) was carried out. Evidences of syn-sedimentary tectonic activity were recognized. The results enabled a better reconstruction of the stratigraphic architecture (with an improved chronostratigraphic resolution) in the framework of the Miocene foredeep evolution of the eastern EBZ. Two main depositional sequences were dated as uppermost Burdigalian-upper Serravallian p.p. and middle-upper Tortonian. p.p., respectively. The vertical and lateral diversification of lithofacies associations and thicknesses resulted from the syn-depositional tectonic complexity of the area. A great variety of sedimentary depositional realms is due to different subsidence rates, and the growing of anticlines and synclines during the Langhian p.p.-Serravallian. After a regression with an early Tortonian erosional gap, platform to hemipelagic realms developed during the middle Tortonian. The end of the sedimentation coincided with the emplacement of an important olisthostrome-like mass consisting of Triassic material related to either the development of thrust systems or diapirs emerged in the middle-late Tortonian, during the nappe emplacement. Correlations with other external sectors of the Betic Chain, and the external domains of the Rif, Tell, and northern Apennine Chains highlighted a similar Miocene foredeep evolution during the building of these orogens.CGL2016-75679-P; Research supported by Grants from Urbino University (Responsible M. Tramontana

Estimation of the light field inside photosynthetic microorganism cultures through Mittag-Leffler functions at depleted light conditions

[EN] Light attenuation within suspensions of photosynthetic microorganisms has been widely described by the Lambert-Beer equation. However, at depths where most of the light has been absorbed by the cells, light decay deviates from the exponential behaviour and shows a lower attenuation than the corresponding from the purely exponential fall. This discrepancy can be modelled through the Mittag-Leffler function, extending Lambert-Beer law via a tuning parameter ¿ that takes into account the attenuation process. In this work, we describe a fractional Lambert-Beer law to estimate light attenuation within cultures of model organism Synechocystis sp. PCC 6803. Indeed, we benchmark the measured light field inside cultures of two different Synechocystis strains, namely the wild-type and the antenna mutant strain called Olive at five different cell densities, with our in silico results. The Mittag-Leffler hyper-parameter ¿ that best fits the data is 0.995, close to the exponential case. One of the most striking results to emerge from this work is that unlike prior literature on the subject, this one provides experimental evidence on the validity of fractional calculus for determining the light field. We show that by applying the fractional Lambert-Beer law for describing light attenuation, we are able to properly model light decay in photosynthetic microorganisms suspensions.This project has received funding from the European Unions Seventh Programme for Research, technological development and demonstration under grant agreement No 308518 CyanoFactory. David Fuente is supported by grant Contratos Predoctorales FPI 2013 of the Universitat Politecnica de Valencia. Carlos Lizama is supported by Programa de Apoyo a la Investigation y Desarrollo (PAID-02-15) de la Universitat Politecnica de Valencia and CONICYT - PIA - Anillo ACT1416Fuente, D.; Lizama, C.; Urchueguía Schölzel, JF.; Conejero, JA. (2018). Estimation of the light field inside photosynthetic microorganism cultures through Mittag-Leffler functions at depleted light conditions. Journal of Quantitative Spectroscopy and Radiative Transfer. 204:23-26. https://doi.org/10.1016/j.jqsrt.2017.08.012S232620