Efeitos do uso tópico do óleo de andiroba puro e ozonizado em feridas induzidas em cavalos

Este trabalho realizou uma avaliação clínica e histopatológica da aplicação tópica do óleo de andiroba (Carapa guianensis Aublet), puro e ozonizado, no processo de cicatrização de feridas em cinco equinos saudáveis. Oito feridas de 6,25 cm2 foram induzidas cirurgicamente, quatro de cada lado da região lombar, craniais em relação à região sacral. Em três animais, o lado esquerdo foi destinado à avaliação macroscópica e mensuração de área, enquanto o lado direito foi destinado à análise histopatológica. Nos outros dois animais, as avaliações foram invertidas. O tratamento tópico foi iniciado 12 horas após a indução cirúrgica e foi mantido diariamente até a completa cicatrização das feridas. Foram usados, sequencialmente e bilateralmente, no sentido craniocaudal: solução salina (GC), solução salina ozonizada (GO), óleo de andiroba puro (GAP) e óleo de andiroba ozonizado (GAO). Aleatoriamente, a sequência de tratamentos foi modificada. As análises macroscópicas e microscópicas foram realizadas 3, 7, 14 e 21 dias após a cirurgia, e o tempo total para cicatrização registrado. A contração da ferida foi de 67,75% para GC, 65,26% para GO, 67,91% para GAP, e 69,84% para GAO. A avaliação histopatológica demonstrou que as feridas tratadas com GAO e GAP apresentaram uma avançada epitelização, proliferação fibroblástica e deposição de colágeno, moderada proliferação vascular e presença de infiltrados de células polimorfonucleares (PMN) e discreta proliferação de células mononucleares (MN). Foi possível concluir que todos os tratamentos usados foram benéficos perante o grupo de controle, mostrando que as versões pura e ozonizada do óleo de andiroba representam alternativas terapêuticas ao tratamento de feridas em equinos.The aim of this study was to evaluate clinical and histopathological aspects of topical application of pure and ozonized andiroba oil (Carapa guianensis Aublet.) on the healing process of wounds of healthy horses. Eight 6.25-cm2 wounds were surgically produced on each horse, cranial to the sacrum, being four wounds on each side of the lumbar region. In three animals, left side was used for macroscopic observations and area measurement and right side was used for histopathological analysis. For the other two animals, evaluations were inverted. Beginning of the topical treatment occurred 12 hours after surgical induction of the injuries and was maintained daily until complete healing of the wounds, using sequentially and bilaterally in the craniocaudal direction saline solution (GC), ozonized saline solution (GO), pure andiroba oil (GAP) and ozonized andiroba oil (GAO). Randomly, sequence of the treatments was modified. Macroscopic and histopathological analyses were performed at 3, 7, 14, and 21 days after surgery. Completed healing time for all wounds were recorded. A wound contraction of 67.75% for GC, 65.26% for GO, 67.91% for GAP, and 69.84% for GAO were recorded. Histopathologic evaluation revealed that wounds from the GAO and GAP had an advanced epithelialization, fibroblast proliferation and collagen deposition, moderate vascular proliferation, and presence of PMN infiltrate and discrete viewing of MN. It was possible to conclude that all treatments had benefits when comparing to control group and both pure and ozonized andiroba oil can be good options for wound treatment in horses

Educar para a sensibilidade estética: uma experiência pedagógica em Educação Física

A abordagem somática da educação reconhece o corpo como fonte de conhecimento, designadamente pelas linguagens estéticas que engloba (Boal, 2009). Já o reconhecimento do valor da corporeidade pelo sistema educativo encontra expressão na disciplina de Educação Física (EF) que acompanha o aluno ao longo de toda a escolaridade obrigatória. Este estudo teve como objetivo analisar o valor atribuído pelos alunos a uma experiência pedagógica no âmbito da estética do desporto através da EF. Participaram no estudo 27 alunos de uma turma do ensino secundário, uma estudante-estagiária, um professor orientador e uma expertise na área da estética do desporto.  Recorreu-se à metodologia qualitativa pela aplicação de entrevistas semiestruturadas individuais e cinco grupos focais, observação participante, complementados com o diário de bodo da professora (investigadora). A análise da informação recolhida permitiu compreender a existência de uma alteração das perspetivas dos alunos sobre questões da estética do desporto. Estes passaram a identificar movimentos fluidos, coordenados e ritmados, o que lhes permitiu melhorar a execução técnica, assim como a capacidade de observação e análise de movimentos, aportando grandes benefícios nos processos de interajuda nas correções técnicas e compreensão dos comportamentos táticos da equipa. O trabalho em equipa também saiu reforçado. Os alunos revelaram ainda que, presentemente, quando assistem a uma competição desportiva prestam mais atenção ao detalhe e à dinâmica das tarefas em equipa

A versatile and compact capacitive dilatometer

We describe the design, construction, calibration, and operation of a relatively simple differential capacitive dilatometer suitable for measurements of thermal expansion and magnetostriction from 300 K to below 1 K with a low-temperature resolution of about 0.05 angstroms. The design is characterized by an open architecture permitting measurements on small samples with a variety of shapes. Dilatometers of this design have operated successfully with a commercial physical property measurement system, with several types of cryogenic refrigeration systems, in vacuum, in helium exchange gas, and while immersed in liquid helium (magnetostriction only) to temperatures of 30 mK and in magnetic fields to 45 T.Comment: 8 pages, incorporating 6 figures, submitted to Rev. Sci. Instru

Effects of topical application of pure and ozonized andiroba oil on experimentally induced wounds in horses

The aim of this study was to evaluate clinical and histopathological aspects of topical application of pure and ozonized andiroba oil (Carapa guianensis Aublet.) on the healing process of wounds of healthy horses. Eight 6.25-cm2 wounds were surgically produced on each horse, cranial to the sacrum, being four wounds on each side of the lumbar region. In three animals, left side was used for macroscopic observations and area measurement and right side was used for histopathological analysis. For the other two animals, evaluations were inverted. Beginning of the topical treatment occurred 12 hours after surgical induction of the injuries and was maintained daily until complete healing of the wounds, using sequentially and bilaterally in the craniocaudal direction saline solution (GC), ozonized saline solution (GO), pure andiroba oil (GAP) and ozonized andiroba oil (GAO). Randomly, sequence of the treatments was modified. Macroscopic and histopathological analyses were performed at 3, 7, 14, and 21 days after surgery. Completed healing time for all wounds were recorded. A wound contraction of 67.75% for GC, 65.26% for GO, 67.91% for GAP, and 69.84% for GAO were recorded. Histopathologic evaluation revealed that wounds from the GAO and GAP had an advanced epithelialization, fibroblast proliferation and collagen deposition, moderate vascular proliferation, and presence of PMN infiltrate and discrete viewing of MN. It was possible to conclude that all treatments had benefits when comparing to control group and both pure and ozonized andiroba oil can be good options for wound treatment in horses

Consequences of eight consecutive applications of equine chorionic gonadotropin on ovarian follicle development and ovulation in bos indicus cows

ABSTRACT: The equine chorionic gonadotropin (eCG) is a glycoprotein produced in mare endometrial calices. In bovine, it is used in estrus synchronization protocols. However, studies have shown that it is potentially immunogenic and its effect can decrease after repetitive use. This study aimed to evaluate antral follicle dynamics, corpus luteum (CL) and ovulation rate in bos indicus cows submitted to an estrus synchronization protocol in association with eCG for eight times consecutively. Ten cyclical, multiparous, and pasture raised beef cows were divided into two groups: control group (n=5) and eCG group (n=5). In 30 day interval, all animals were synchronized with the same estrogen/progesterone based protocol, totalizing 8 re-synchronizations. Cows in the treatment group received 300IU eCG 48 hours prior to the presumable ovulation. Ultrassound examinations were performed on Day 4 of the protocol (approximately 1.5 days after follicle recruitment) to count antral follicles, on Day 10, to count antral follicles and to measure size of the largest follicle and on Day 18 to measure the diameter of the CL. No difference (P>0.05) between follicular growth and size of the pre-ovulatory follicle was reported between groups. Cows treated with eCG had a larger (P<0.05) CL and increased (P<0.05) ovulation rate (18mm and 92%, respectively) when compared with control group (14.1mm and 80%, respectively). Furthermore, consecutive treatments did not affect CL nor ovulation rates. In conclusion, eCG treatment increased CL size and ovulation rate even after 8 consecutive treatments

Utilização de ultrassonografia modo doppler como diagnóstico complementar da endometrite em éguas: relato de caso

The aim of this study was to report the use of Doppler ultrasound as a complementary tool for the diagnosis of endometritis in mares. Seven cross-bred recipient mares, showing mild vaginal discharge, were subjected to gynecological exams (cytology, bacteriological examination and B-mode ultrasonography) and to Doppler ultrasound. The use of Doppler ultrasound allowed a more accurate assessment of the endometrial vascular perfusion condition and enabled the observation of vascular alterations, confirming the presence of inflammation, demonstrating that Doppler ultrasonography is a valid tool for the complementary diagnosis of bacteriological endometritis in mares.O objetivo deste trabalho foi relatar a utilização da ultrassonografia modo Doppler como ferramenta complementar no diagnóstico da endometrite em éguas. Para tal, sete éguas receptoras mestiças, apresentando corrimento vaginal leve, foram submetidas a exames ginecológicos (avaliação citológica, bacteriológica e ultrassonografia modo-B) e à ultrassonografia modo Doppler. A utilização da ultrassonografia modo Doppler permitiu uma avaliação mais precisa da condição da perfusão vascular do endométrio e foi capaz de confirmar a presença da inflamação pela observação das alterações vasculares, sendo uma ferramenta válida para o diagnóstico complementar da endometrite bacteriana

Versatile and compact capacitive dilatometer

We describe the design, construction, calibration, and operation of a relatively simple differential capacitive dilatometer suitable for measurements of thermal expansion and magnetostriction from 300 to below 1K with a low-temperature resolution of about 0.05Å. The design is characterized by an open architecture permitting measurements on small samples with a variety of shapes. Dilatometers of this design have operated successfully with a commercial physical property measurement system, with several types of cryogenic refrigeration systems, in vacuum, in helium exchange gas, and while immersed in liquid helium(magnetostriction only) to temperatures of 30mK and in magnetic fields to 45T.The following article appeared in Review of Scientific Instruments 77 (2006): 123907 and may be found at http://dx.doi.org/10.1063/1.2403088.</p

Fungi predatory activity on embryonated Toxocara canis eggs inoculated in domestic chickens (Gallus gallus domesticus) and destruction of second stage larvae

The objective of this study was to evaluate the infectivity of Toxocara canis eggs after interacting with isolated nematophagous fungi of the species Duddingtonia flagrans (AC001) and Pochonia chlamydosporia (VC4), and test the predatory activity of the isolated AC001 on T. canis second stage larvae after 7 days of interaction. In assay A, 5000 embryonated T. canis eggs previously in contact with the AC001 and VC4 isolated for 10 days were inoculated into domestic chickens (Gallus gallus domesticus), and then these animals were necropsied to collect material (digested liver, intestine, muscles and lungs) at 3-, 7-, 14-, and 21-day intervals after inoculation. In assay A, the results demonstrated that the prior interaction of the eggs with isolated AC001 and VC4 decreases the amount of larvae found in the collected organs. Difference (p < 0.01) was observed in the medium larvae counts recovered from liver, lung, intestine, and muscle of animals in the treated groups when compared to the animals in the control group. At the end of assay A, a percentage reduction of 87.1 % (AC001) and 84.5 % (VC4) respectively was recorded. In the result of assay B, the isolated AC001 showed differences (p < 0.01) compared to the control group, with a reduction of 53.4 % in the recovery of L2. Through these results, it is justified to mention that prior interaction of embryonated T. canis eggs with the tested fungal isolates were efficient in reducing the development and migration of this parasite, in addition to the first report of proven predatory activity on L2