Colorado’s implementation of legal marijuana policies has been a patchwork of regulation against a backdrop of diverse public opinion.

The recreational use of marijuana in Colorado has now been legal for more than three years, but cities, municipalities and counties have had the freedom to choose whether or not to legalize the drug or for it to remain banned. In new research using survey data and panel interviews with Coloradan local administrators, Tracy L. Johns finds that in their decision making these officials had to consider often divided local public opinion against the potential economic costs and benefits of legalization and the workload of enforcing marijuana policies

Preferred Information Channels and Source Trustworthiness: Assessing Communication Methods Used in Florida\u27s Battle Against Citrus Greening

The purpose of this study was to examine the perceived source credibility, as viewed by Florida citrus growers, of state and national agricultural organizations associated with containing the plant disease called citrus greening. This study sought to determine the types of information that agricultural opinion leaders received from these agriculture organizations. In late 2007, three focus groups were conducted at county Cooperative Extension Service offices in three major areas of citrus production. The number of participants ranged from four to six for a total of 15. Growers were asked about their awareness and understanding of citrus greening; management practices; cooperation with fellow producers and institutions that played a role in citrus greening management; preferred avenues of communication; and information dissemination methods. Qualitative analysis was conducted to identify key themes and patterns within each topic category. Focus group results indicated that Florida citrus growers preferred to receive information about citrus greening primarily through face-to-face meetings and field days. They also preferred to receive their information from other growers in order to find out how their fellow farmers handled citrus greening. Focus group participants struggled with trusting regulators and university researchers, based on the growers’ previous experiences during the unsuccessful citrus canker eradication program and growers’ perceptions of the practicality of the research. Finally, growers were critical of the lack of available information about citrus greening from regulators and university researchers

A study of the multiple perspective approach to leadership used by elementary and secondary public school principals in urban Iowa

Based on the writings and research of Dr. L. G. Bolman and Dr. T. E. Deal, this study sought to assess the leadership practices, preferences, preparedness, and performance of public school principals in urban Iowa. Specifically, were the principals in Iowa\u27s eight largest districts making use of a multiple perspective approach to leadership? In what areas did they appear to feel most competent? And, in what areas did the principals in this study appear to feel most inadequate? Bolman and Deal\u27s Leadership Orientations ( Self ) Survey (1990) was distributed to 240 elementary and secondary principals working within the Urban Education Network of Iowa in April of 2002. With 126 surveys completed and returned, this study proceeded with a 52.5% response rate. The following conclusions were drawn based on the results of this study: (a) the human resource frame was the frame of choice among the respondents in this study; (b) the structural frame was the second frame of choice among a majority of the respondents in this study; (c) the political frame and the symbolic frame were used less often than the human resource frame and the structural frame; (d) less than one half (40.5%) of the respondents in this study reported themselves to be using a “multiple perspective” approach to leadership; (e) gender, age, experience, and level did not significantly influence frame use among the respondents in this study; (f) although correlations between the score on the leadership effectiveness self-rating, the managerial effectiveness self-rating, and frame use were found to be statistically significant, little or no practical significance could be found within the data; and (g) the respondents in this study reported themselves to be more effective as managers rather than leaders. Overall, the findings in this study of Iowa\u27s urban principals were consistent with the research of Bolman and Deal

A critical role for lymphatic endothelial heparan sulfate in lymph node metastasis

Abstract Background Lymph node metastasis constitutes a key event in tumor progression. The molecular control of this process is poorly understood. Heparan sulfate is a linear polysaccharide consisting of unique sulfate-modified disaccharide repeats that allow the glycan to bind a variety of proteins, including chemokines. While some chemokines may drive lymphatic trafficking of tumor cells, the functional and genetic importance of heparan sulfate as a possible mediator of chemokine actions in lymphatic metastasis has not been reported. Results We applied a loss-of-function genetic approach employing lymphatic endothelial conditional mutations in heparan sulfate biosynthesis to study the effects on tumor-lymphatic trafficking and lymph node metastasis. Lymphatic endothelial deficiency in N-deacetylase/N-sulfotransferase-1 (Ndst1), a key enzyme involved in sulfating nascent heparan sulfate chains, resulted in altered lymph node metastasis in tumor-bearing gene targeted mice. This occurred in mice harboring either a pan-endothelial Ndst1 mutation or an inducible lymphatic-endothelial specific mutation in Ndst1. In addition to a marked reduction in tumor metastases to the regional lymph nodes in mutant mice, specific immuno-localization of CCL21, a heparin-binding chemokine known to regulate leukocyte and possibly tumor-cell traffic, showed a marked reduction in its ability to associate with tumor cells in mutant lymph nodes. In vitro modified chemotaxis studies targeting heparan sulfate biosynthesis in lymphatic endothelial cells revealed that heparan sulfate secreted by lymphatic endothelium is required for CCL21-dependent directional migration of murine as well as human lung carcinoma cells toward the targeted lymphatic endothelium. Lymphatic heparan sulfate was also required for binding of CCL21 to its receptor CCR7 on tumor cells as well as the activation of migration signaling pathways in tumor cells exposed to lymphatic conditioned medium. Finally, lymphatic cell-surface heparan sulfate facilitated receptor-dependent binding and concentration of CCL21 on the lymphatic endothelium, thereby serving as a mechanism to generate lymphatic chemokine gradients. Conclusions This work demonstrates the genetic importance of host lymphatic heparan sulfate in mediating chemokine dependent tumor-cell traffic in the lymphatic microenvironment. The impact on chemokine dependent lymphatic metastasis may guide novel therapeutic strategies

The importance of pro-social processing, and ameliorating dysfunction in schizophrenia. An FMRI study of oxytocin

Schizophrenia is often a severe and debilitating mental illness, frequently associated with impairments in social cognition that hinder individuals' abilities to relate to others and integrate effectively in society. Oxytocin has emerged as a putative therapeutic agent for treating social deficits in schizophrenia, but the mode of action remains unclear. This placebo-controlled crossover study aimed to elucidate the neural underpinnings of oxytocin administration in patients with schizophrenia. 20 patients with schizophrenia were examined using functional magnetic resonance imaging under oxytocin (40 IU) or placebo nasal spray. Participants performed a stochastically rewarded decision-making task that incorporated elements of social valence provided by different facial expressions, i.e. happy, angry and neutral. Oxytocin attenuated the normal bias in selecting the happy face accompanied by reduced activation in a network of brain regions that support mentalising, processing of facial emotion, salience, aversion, uncertainty and ambiguity in social stimuli, including amygdala, temporo-parietal junction, posterior cingulate cortex, precuneus and insula. These pro-social effects may contribute to the facilitation of social engagement and social interactions in patients with schizophrenia and warrant further investigation in future clinical trials for social cognitive impairments in schizophrenia

Peers Emotionality and Childrens Academic Achievement in Second Grade: Testing the Moderating Role of Childrens Behavioral Self-Regulation.

Although there is interest in the role of peers in childrens schooling experiences, few researchers have examined associations and related underlying processes between peers emotionality, an aspect of temperament, and childrens academic achievement. This study evaluated whether target childrens (N = 260) own self-regulation, assessed with two behavioral measures, served a moderating function for associations between peers emotionality and childrens own academic achievement in second grade. There was a positive association between peers positive emotionality and reading scores for children with higher self-regulation. Peers negative emotionality was negatively related to target childrens reading scores, particularly for children with higher self-regulation levels, but was unrelated to math scores. Peers positive and negative emotionality did not predict math scores, and there was no strong evidence for the moderating role of target childrens self-regulation in this association. This study highlights the potential role of childrens self-regulation in modulating peer effects on academic achievement, particularly reading

Prediction of Children\u27s Early Academic Adjustment from Their Temperament: The Moderating Role of Peer Temperament

The goal of the study was to examine whether target children’s temperamental negative emotional expressivity (NEE) and effortful control in the fall of kindergarten predicted academic adjustment in the spring and whether a classmate’s NEE and effortful control moderated these relations. Target children’s NEE and effortful control were measured in the fall via multiple methods, academic adjustment was measured via reading and math standardized tests in the spring, and observations of engagement in the classroom were conducted throughout the year. In the fall, teachers nominated a peer with whom each target child spent the most time and rated that peer’s temperament. Target children with high effortful control had high reading and math achievement (ps = .04 and \u3c .001, respectively), and children with low NEE increased in engagement during the year (p \u3c .001). Peers’ temperament did not have a direct relation to target children’s academic adjustment. Peers’ NEE, however, moderated the relation between target children’s effortful control, as well as NEE, and changes in engagement (ps = .03 and .05, respectively). Further, peers’ effortful control moderated the relations between target children’s NEE and reading and changes in engagement (ps = .02 and .04, respectively). In each case, target children’s temperament predicted the outcome in expected directions more strongly when peers had low NEE or high effortful control. Results are discussed in terms of how children’s temperamental qualities relate to academic adjustment, and how the relation between NEE and changes in engagement, in particular, depends on peers’ temperament

Chlamydia trachomatis genotypes in a cross-sectional study of urogenital samples from remote Northern and Central Australia

his is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/Abstract OBJECTIVES: The objective was to determine the frequency of trachoma genotypes of Chlamydia trachomatis-positive urogenital tract (UGT) specimens from remote areas of the Australian Northern Territory (NT). SETTING: The setting was analysis of remnants of C. trachomatis positive primarily UGT specimens obtained in the course of clinical practice. The specimens were obtained from two pathology service providers. PARTICIPANTS: From 3356 C. trachomatis specimens collected during May 2012-April 2013, 439 were selected for genotyping, with a focus on specimens from postpubescent patients, in remote Aboriginal communities where ocular trachoma is potentially present. PRIMARY AND SECONDARY OUTCOME MEASURES: The primary outcome measure was the proportion of successfully genotyped UGT specimens that were trachoma genotypes. The secondary outcome measures were the distribution of genotypes, and the frequencies of different classes of specimens able to be genotyped. RESULTS: Zero of 217 successfully genotyped UGT specimens yielded trachoma genotypes (95% CI for frequency=0-0.017). For UGT specimens, the genotypes were E (41%), F (22%), D (21%) and K (7%), with J, H and G and mixed genotypes each at 1-4%. Four of the five genotyped eye swabs yielded trachoma genotype Ba, and the other genotype J. Two hundred twenty-two specimens (50.6%) were successfully genotyped. Urine specimens were less likely to be typable than vaginal swabs (p<0.0001). CONCLUSIONS: Unlike in some other studies, in the remote NT, trachoma genotypes of C. trachomatis were not found circulating in UGT specimens from 2012 to 2013. Therefore, C. trachomatis genotypes in UGT specimens from young children can be informative as to whether the organism has been acquired through sexual contact. We suggest inclusion of C. trachomatis genotyping in guidelines examining the source of sexually transmitted infections in young children in areas where trachoma genotypes may continue to circulate, and continued surveillance of UGT C. trachomatis genotypes

The importance of pro-social processing, and ameliorating dysfunction in schizophrenia. An FMRI study of oxytocin

Schizophrenia is often a severe and debilitating mental illness, frequently associated with impairments in social cognition that hinder individuals' abilities to relate to others and integrate effectively in society. Oxytocin has emerged as a putative therapeutic agent for treating social deficits in schizophrenia, but the mode of action remains unclear. This placebo-controlled crossover study aimed to elucidate the neural underpinnings of oxytocin administration in patients with schizophrenia. 20 patients with schizophrenia were examined using functional magnetic resonance imaging under oxytocin (40 IU) or placebo nasal spray. Participants performed a stochastically rewarded decision-making task that incorporated elements of social valence provided by different facial expressions, i.e. happy, angry and neutral. Oxytocin attenuated the normal bias in selecting the happy face accompanied by reduced activation in a network of brain regions that support mentalising, processing of facial emotion, salience, aversion, uncertainty and ambiguity in social stimuli, including amygdala, temporo-parietal junction, posterior cingulate cortex, precuneus and insula. These pro-social effects may contribute to the facilitation of social engagement and social interactions in patients with schizophrenia and warrant further investigation in future clinical trials for social cognitive impairments in schizophrenia