Sex ratio and gamete size across eastern North America in Dictyostelium discoideum, a social amoeba with three sexes

Theory indicates that numbers of mating types should tend towards infinity or remain at two. The social amoeba, Dictyostelium discoideum, however, has three mating types. It is therefore a mystery how this species has broken the threshold of two mating types, but has not increased towards a much higher number. Frequency-dependent selection on rare types in combination with isogamy, a form of reproduction involving gametes similar in size, could explain the evolution of multiple mating types in this system. Other factors, such as drift, may be preventing the evolution of more than three. We first looked for evidence of isogamy by measuring gamete size associated with each type. We found no evidence of size dissimilarities between gametes. We then looked for evidence of balancing selection, by examining mating type distributions in natural populations and comparing genetic differentiation at the mating type locus to that at more neutral loci. We found that mating type frequency varied among the three populations we examined, with only one of the three showing an even sex ratio, which does not support balancing selection. However, we found more population structure at neutral loci than the mating type locus, suggesting that the three mating types are indeed maintained at intermediate frequencies by balancing selection. Overall, the data are consistent with balancing selection acting on D. discoideum mating types, but with a sufficiently weak rare sex advantage to allow for drift, a potential explanation for why these amoebae have only three mating types

Field Test Performance of Junior Competitive Surf Athletes following a Core Strength Training Program

International Journal of Exercise Science 11(6): 696-707, 2018. Lower body and core muscular strength are essential for optimal performance in many sports and competitive surfers have similar strength demands when maneuvering a surfboard to achieve competition success. Presently, the use of unstable surfaces is excessively utilized by surf coaches and trainers and to date, research does not support this as an effective training method for long-term improvements. Therefore, the purpose of this study was to determine the effectiveness of an 8-week Core Strength Training Program (CSTP) on a battery of field tests specific to assessing core musculature and lower body strength for junior competitive surf athletes. Nineteen American junior competitive surf athletes (age:15.7±1.01yrs, height:1.77±0.007m, mass:64.67±9.08kg) completed pre- and post-tests with a transitional pre-season to in-season 8-week CSTP intervention. The battery of tests included: rotational power (RP), time to peak acceleration (TP), maximal acceleration (Ma), maximal countermovement jump (CMJ), estimated peak power (PP), core strength (CS), core endurance (CE), and rotational flexibility (RF). Means, standard deviations, RMANOVA with a significance level of p \u3c 0.05, and effect sizes were computed. Results demonstrated significant improvements in L.RP, TP, CMJ, PP, CS, and RF. Based on the results, the CSTP is an effective training program for surf coaches and strength and conditioning professionals to improve strength in the core musculature and lower body. In addition, we conclude implementation of the CSTP enhances athletic performance measurements which will likely increase competition success

Fredholm Determinants, Differential Equations and Matrix Models

Orthogonal polynomial random matrix models of NxN hermitian matrices lead to Fredholm determinants of integral operators with kernel of the form (phi(x) psi(y) - psi(x) phi(y))/x-y. This paper is concerned with the Fredholm determinants of integral operators having kernel of this form and where the underlying set is a union of open intervals. The emphasis is on the determinants thought of as functions of the end-points of these intervals. We show that these Fredholm determinants with kernels of the general form described above are expressible in terms of solutions of systems of PDE's as long as phi and psi satisfy a certain type of differentiation formula. There is also an exponential variant of this analysis which includes the circular ensembles of NxN unitary matrices.Comment: 34 pages, LaTeX using RevTeX 3.0 macros; last version changes only the abstract and decreases length of typeset versio

High Spatial Resolution X-Ray Spectroscopy of the IC443 Pulsar Wind Nebula and Environs

Deep Chandra ACIS observations of the region around the putative pulsar, CXOU J061705.3+222127, in the supernova remnant IC443 reveal an ~5$^{\prime\prime}$-radius ring-like structure surrounding the pulsar and a jet-like feature oriented roughly north-south across the ring and through the pulsar's location at 06$^{\rm h}$17$^{\rm m}$5.200$^{\rm s}$ +22$^{\circ}$21$^{\prime}$27.52$^{\prime\prime}$ (J2000.0 coordinates). The observations further confirm that (1) the spectrum and flux of the central object are consistent with a rotation-powered pulsar, (2) the non-thermal spectrum and morphology of the surrounding nebula are consistent with a pulsar wind and, (3) the spectrum at greater distances is consistent with thermal emission from the supernova remnant. The cometary shape of the nebula, suggesting motion towards the southwest, appears to be subsonic: There is no evidence either spectrally or morphologically for a bow shock or contact discontinuity; the nearly circular ring is not distorted by motion through the ambient medium; and the shape near the apex of the nebula is narrow. Comparing this observation with previous observations of the same target, we set a 99% confidence upper limit to the proper motion of CXOU J061705.3+222127 to be less than 44 mas/yr (310 km/s for a distance of 1.5 kpc), with the best-fit (but not statistically significant) projected direction toward the west.Comment: Accepted for publication in the Astrophysical Journa

Bolocam Survey for 1.1 mm Dust Continuum Emission in the c2d Legacy Clouds. II. Ophiuchus

We present a large-scale millimeter continuum map of the Ophiuchus molecular cloud. Nearly 11 square degrees, including all of the area in the cloud with visual extinction more than 3 magnitudes, was mapped at 1.1 mm with Bolocam on the Caltech Submillimeter Observatory (CSO). By design, the map also covers the region mapped in the infrared with the Spitzer Space Telescope. We detect 44 definite sources, and a few likely sources are also seen along a filament in the eastern streamer. The map indicates that dense cores in Ophiuchus are very clustered and often found in filaments within the cloud. Most sources are round, as measured at the half power point, but elongated when measured at lower contour levels, suggesting spherical sources lying within filaments. The masses, for an assumed dust temperature of 10 K, range from 0.24 to 3.9 solar masses, with a mean value of 0.96 solar masses. The total mass in distinct cores is 42 solar masses, 0.5 to 2% of the total cloud mass, and the total mass above 4 sigma is about 80 solar masses. The mean densities in the cores are quite high, with an average of 1.6 x 10^6 per cc, suggesting short free-fall times. The core mass distribution can be fitted with a power law with slope of 2.1 plus or minus 0.3 for M>0.5 solar masses, similar to that found in other regions, but slightly shallower than that of some determinations of the local IMF. In agreement with previous studies, our survey shows that dense cores account for a very small fraction of the cloud volume and total mass. They are nearly all confined to regions with visual extinction at least 9 mag, a lower threshold than found previously.Comment: 47 pages, 16 figures, accepted for Ap

Medical Biochemistry Without Rote Memorization: Multi-Institution Implementation And Student Perceptions Of A Nationally Standardized Metabolic Map For Learning And Assessment

Despite the growing number of patients worldwide with metabolism-related chronic diseases, medical biochemistry education is commonly perceived as focusing on recall of facts irrelevant for patient care. The authors suggest that this focus on rote memorization of pathways creates excessive cognitive load that may interfere with learners’ development of an integrated understanding of metabolic regulation and dysregulation. This cognitive load can be minimized by providing appropriate references during learning and assessment. Biochemistry educators collaborated to develop a medically relevant Pathways of Human Metabolism map (MetMap) that is now being used at many medical schools as a nationally standardized resource during learning and assessments. To assess impact, students from three medical schools were surveyed about its benefits and disadvantages. Responses were obtained from 481 students (84%) and were examined using thematic analysis. Five main themes emerged as perceived benefits of using the MetMap: 1) aids visual and mental organization, 2) promotes deep learning and applied understanding, 3) decreases emphasis on memorization, 4) reduces anxiety on exams, and 5) aids recall. Perceived disadvantages were: 1) fear of under-preparation for licensing exams, 2) overwhelming nature of the map, and 3) reduced motivation for and time spent studying. Results affirm that students perceive use of the MetMap promotes focus on broader metabolic concepts and deep versus surface learning, supporting a shift in cognitive load toward desired goals. Although the long-term impact on learning needs to be further studied, the use of the MetMap represents a step toward open-reference exams that reflect “real world” practice

Differences in coronary flow and myocardial metabolism at rest and during pacing between patients with obstructive and patients with nonobstructive hypertrophic cardiomyopathy

Fifty patients with hypertrophic cardiomyopathy underwent invasive study of coronary and myocardial hemodynamics in the basal state and during the stress of pacing. The 23 patients with basal obstruction (average left ventricular outflow gradient, 77 ± 33 mm Hg; left ventricular systolic pressure, 196 ± 33 mm Hg, mean ± 1 SD) had significantly lower coronary resistance (0.85 ± 0.18 versus 1.32 ± 0.44 mm Hg min/ml, p < 0.001) and higher basal coronary flow (106 ± 20 versus 80 ± 25 ml/min, p < 0.001) in the anterior left ventricle, associated with higher regional myocardial oxygen consumption (12.4 ± 3.6 versus 8.9 ± 3.3 ml oxygen/min, p < 0.001) compared with the 27 patients without obstruction (mean left ventricular systolic pressure 134 ± 18 mm Hg, p < 0.001).Myocardial oxygen consumption and coronary blood flow were also significantly higher at paced heart rates of 100 and 130 beats/min (the anginal threshold for 41 of the 50 patients) in patients with obstruction compared with those without. In patients with obstruction, transmural coronary flow reserve was exhausted at a heart rate of 130 beats/min; higher heart rates resulted in more severe metabolic evidence of ischemia with all patients experiencing chest pain, associated with an actual increase in coronary resistance. Patients without obstruction also demonstrated evidence of ischemia at heart rates of 130 and 150 beats/min, with 25 of 27 patients experiencing chest pain. In this group, myocardial ischemia occurred at significantly lower coronary flow, higher coronary resistance and lower myocardial oxygen consumption, suggesting more severely impaired flow delivery in this group compared with those with obstruction. Abnormalities in myocardial oxygen extraction and marked elevation in filling pressures during stress were noted in both groups.Thus, obstruction to left ventricular outflow is associated with high left ventricular systolic pressure and oxygen consumption and therefore has important pathogenetic importance to the precipitation of ischemia in patients with hypertrophic cardiomyopathy. Patients without obstruction may have greater impairment in coronary flow delivery during stress

The Wasteland of Random Supergravities

We show that in a general \cal{N} = 1 supergravity with N \gg 1 scalar fields, an exponentially small fraction of the de Sitter critical points are metastable vacua. Taking the superpotential and Kahler potential to be random functions, we construct a random matrix model for the Hessian matrix, which is well-approximated by the sum of a Wigner matrix and two Wishart matrices. We compute the eigenvalue spectrum analytically from the free convolution of the constituent spectra and find that in typical configurations, a significant fraction of the eigenvalues are negative. Building on the Tracy-Widom law governing fluctuations of extreme eigenvalues, we determine the probability P of a large fluctuation in which all the eigenvalues become positive. Strong eigenvalue repulsion makes this extremely unlikely: we find P \propto exp(-c N^p), with c, p being constants. For generic critical points we find p \approx 1.5, while for approximately-supersymmetric critical points, p \approx 1.3. Our results have significant implications for the counting of de Sitter vacua in string theory, but the number of vacua remains vast.Comment: 39 pages, 9 figures; v2: fixed typos, added refs and clarification

PAMELA, DAMA, INTEGRAL and Signatures of Metastable Excited WIMPs

Models of dark matter with ~ GeV scale force mediators provide attractive explanations of many high energy anomalies, including PAMELA, ATIC, and the WMAP haze. At the same time, by exploiting the ~ MeV scale excited states that are automatically present in such theories, these models naturally explain the DAMA/LIBRA and INTEGRAL signals through the inelastic dark matter (iDM) and exciting dark matter (XDM) scenarios, respectively. Interestingly, with only weak kinetic mixing to hypercharge to mediate decays, the lifetime of excited states with delta < 2 m_e is longer than the age of the universe. The fractional relic abundance of these excited states depends on the temperature of kinetic decoupling, but can be appreciable. There could easily be other mechanisms for rapid decay, but the consequences of such long-lived states are intriguing. We find that CDMS constrains the fractional relic population of ~100 keV states to be <~ 10^-2, for a 1 TeV WIMP with sigma_n = 10^-40 cm^2. Upcoming searches at CDMS, as well as xenon, silicon, and argon targets, can push this limit significantly lower. We also consider the possibility that the DAMA excitation occurs from a metastable state into the XDM state, which decays via e+e- emission, which allows lighter states to explain the INTEGRAL signal due to the small kinetic energies required. Such models yield dramatic signals from down-scattering, with spectra peaking at high energies, sometimes as high as ~1 MeV, well outside the usual search windows. Such signals would be visible at future Ar and Si experiments, and may be visible at Ge and Xe experiments. We also consider other XDM models involving ~ 500 keV metastable states, and find they can allow lighter WIMPs to explain INTEGRAL as well.Comment: 22 pages, 7 figure