Biochemical and functional characterization of proteins encoded by SARS-CoV subgenomic mRNA 8

Master'sMASTER OF SCIENC

Spécification d'un jeu pour l'apprentissage de l'écriture chinoise

En 2015, dans le cadre du projet Innovalangues (Université Stendhal), le lot GAMER, en collaboration avec l’équipe de mandarin du lot SELF, a lancé le projet « Jeu en chinois ». Ce projet vise à la conception et au développement d’un jeu numérique pour l’apprentissage de l’écriture chinoise, autour de la notion de sinogrammes. Ce mémoire est un compte-rendu analytique d’un stage effectué au sein de ce projet. Ce rapport comprend une partie théorique dédiée à la didactique du chinois, une autre concernant les choix de stratégies de réalisation et mécanique de jeu (jeu d’association : Candy Crush Saga), et une dernière présentant la première ébauche du jeu (nommé « Kanji Crunch »)

STRUCTURAL ANALYSIS OF RIBOSOME BINDING ELEMENTS OF THE 3´ UTR IN TWO POSITIVE-STRAND PLANT RNA VIRUSES

Turnip crinkle virus (TCV) and Pea enation mosaic virus (PEMV) are two positive (+)-strand RNA viruses that are used to investigate the regulation of translation and replication due to their small size and simple genomes. Both viruses contain cap-independent translation elements (CITEs) within their 3´ untranslated regions (UTRs) that fold into tRNA-shaped structures (TSS) according to nuclear magnetic resonance and small angle x-ray scattering analysis (TCV) and computational prediction (PEMV). Specifically, the TCV TSS can directly associate with ribosomes and participates in RNA-dependent RNA polymerase (RdRp) binding. The PEMV kissing-loop TSS (kl-TSS) can simultaneously bind to ribosomes and associate with the 5´ UTR of the viral genome. Mutational analysis and chemical structure probing methods provide great insight into the function and secondary structure of the two 3´ CITEs. However, lack of 3-D structural information has limited our understanding of their functional dynamics. Here, I report the folding dynamics for the TCV TSS using optical tweezers (OT), a single molecule technique. My study of the unfolding/folding pathways for the TCV TSS has provided an unexpected unfolding pathway, confirmed the presence of Ψ3 and hairpin elements, and suggested an interconnection between the hairpins and pseudoknots. In addition, this study has demonstrated the importance of the adjacent upstream adenylate-rich sequence for the formation of H4a/Ψ3 along with the contribution of magnesium to the stability of the TCV TSS. In my second project, I report on the structural analysis of the PEMV kl-TSS using NMR and SAXS. This study has re-confirmed the base-pair pattern for the PEMV kl-TSS and the proposed interaction of the PEMV kl-TSS with its interacting partner, hairpin 5H2. The molecular envelope of the kl-TSS built from SAXS analysis suggests the kl-TSS has two functional conformations, one of which has a different shape from the previously predicted tRNA-shaped form. Along with applying biophysical methods to study the structural folding dynamics of RNAs, I have also developed a technique that improves the production of large quantities of recombinant RNAs in vivo for NMR study. In this project, I report using the wild-type and mutant E.coli strains to produce cost-effective, site-specific labeled, recombinant RNAs. This technique was validated with four representative RNAs of different sizes and complexity to produce milligram amounts of RNAs. The benefit of using site-specific labeled RNAs made from E.coli was demonstrated with several NMR techniques

Factors Influencing Adoption of Online Payment via E-Commerce Platforms

In this study, the research group applied the Technology Acceptance Model (TAM) by Davis (1989) to analyze factors influencing customers’ intention to use the online payment via e-commerce platforms as well as to encourage Vietnamese consumers to adapt this means of payment. Besides Perceived Ease of Use and Perceived Usefulness in the original model of Davis (1989), three additional dependent factors (Subjective Norm, Trust, Risk) were added to the model. The new model proposed by the research group aims to give a more well-rounded explanation on Vietnamese consumers’ attitude and intention towards the online payment. Using sample data including 448 consumers in the Northern region of Vietnam, the research group has been able to validate the hypothesis model and prove that independent and dependent variables (Attitude towards Use and Intention to Use) are positively correlated. Among the 5 dependent variables, Subjective Norm is found to be the most significant factor contributing to promote online payment attitude and intention through e-commerce websites of Vietnamese consumers. Keywords: TAM, online payment, online shopping, e-commerce DOI: 10.7176/EJBM/13-8-19 Publication date: April 30th 202

Performance of Self-compacting Concrete with Stainless Steel Slag Versus Fly Ash as Fillers: A Comparative Study

Recently, stainless steel slag -a byproduct of manufacturing stainless steel is accepted as a cementitious material, the chemical characteristics of which are highly variant. This study reuses two types of stainless steel reducing slag with specific surface area of 1766 cm2/g (S1) and 7970 cm2/g (S2) in developing self-compacting concrete (SCC). Particularly, two S2-blended SCCs incorporating with S1 and fly ash as fillers (calling as S-mix and F-mix) were prepared for a comparative investigation. In both SCCs, ordinary Portland cement was replaced by S2 with various ratios (from 0 % to 50 %, increment 10 %). Testing results show that in fresh state, the F-mix exhibits higher workability and longer initial setting time than those of S-mix. In hardened state, 10 % compressive strength loss was realized as increasing S2 content up to 30 % in the both SCCs; the strength of F-mix is up to 1.9 times of S-mix at the same rate of S2 replacement. Water absorption of the F-mix was below 3 %, suggested as a “good” quality concrete; whilst the S-mix could be longs to an “average” one. Resistivity and sulfate resistance of F-mix are considerably higher than those of S-mix. Moreover, based on the obtained data, compressive strength and electrical resistivity are correlated well with a logarithmic form

Empowering Vietnamese EFL Learners at Tertiary Level: Investigating Factors Shaping Learner Autonomy in English Language Acquisition

This study scrutinizes the viewpoints of Vietnamese students studying English as a Foreign Language (EFL) regarding determinants impacting their autonomy in English language acquisition. Data was amassed from 117 Vietnamese EFL learners attending a university in Vietnam by using a survey-based methodology. The survey instrument, comprising Likert-type items, was predicated on well-established conceptual frameworks derived from antecedent studies. The study unveiled multiple key influences on learner autonomy, incorporating voluntariness, learner choice, adaptability in study alternatives, peer collaboration, and beliefs about the educator’s role, motivation, capabilities, and independence. These determinants underscore the significance of learners’ active participation, personal agency, social interactions, nurturing learning ecosystems, intrinsic motivation, efficacious learning strategies, and learner empowerment in cultivating learner autonomy. The implications gleaned from these results emphasize the necessity for pedagogical stratagems that encourage voluntariness, learner choice, peer collaboration, teacher backing, motivation, skill enhancement, and learner agency within English language educational environments. These pedagogical stratagems would better equip learners for the autonomous journey of English language acquisition, thereby enhancing the overall efficiency of English language education in the Vietnamese context

HMU fluorinze mouthwash enhances enamel remineralization: An in vitro study

BACKGROUND: Fluoride therapy has long been used extensively to prevent dental caries. Fluoride appears in variety of dental care products such as mouthrinse, dentifrice, gel, etc. HMU fluorinze is the first mouthwash containing fluoride in Vietnam. AIM: This research was conducted to evaluate the efficacy of HMU Fluorinze mouthwash on remineralizing enamel in laboratory conditions. METHODS: 20 third molars teeth were cleaned and covered with nail polish , except for a 3x3 mm square on their buccal surfaces. These teeth went through two steps: demineralization using Coke and remineralization for 20 days: 1) using standard calcifying solution (control group) and 2) using standard calcifying solution + HMU Fluorinze mouthwash 2 times/day (experimental group). The mineralization index of enamel structure after demineralization and remineralization was assessed by DIAGNOdent pen 2190. RESULTS: The mineralization indexes of the control group and experimental group at baseline were 3.65 ± 0.76 and 3.35 ± 0.64, after demineralization were in turn of 21.78 ± 4.48 and 20.25 ± 2.26; and after remineralization were 6.30 ± 1.03 and 3.90 ± 1.24. The different figures  between the two groups after remineralization shows statistical significance (p<0.01). Group B using HMU fluorinze mouthwash after 20 days did not differ from the original results (p = 0.272), in contrast with the control group (p<0.01). CONCLUSIONS: HMU fluorinze mouthwash has better mineralization effect than standard calcifying solution

The Emergence of Rotavirus G12 and the Prevalence of Enteric Viruses in Hospitalized Pediatric Diarrheal Patients in Southern Vietnam

Diarrhea is a major cause of childhood morbidity and mortality in developing countries, and the majority of infections are of viral etiology. We aimed to compare the etiological prevalence of the major enteric viruses in an urban and a rural setting in southern Vietnam. We simultaneously screened fecal specimens from 362 children in Ho Chi Minh City and Dong Thap province that were hospitalized with acute diarrhea over a 1-month-long period for four viral gastrointestinal pathogens. Rotavirus was the most common pathogen identified, but there was a differential prevalence of rotavirus and norovirus between the urban and rural locations. Furthermore, rotavirus genotyping and phylogenetic analysis again differentiated the genotypes by the sampling location. Our data show a disproportional distribution of enteric viral pathogens in urban and rural locations, and we provide evidence of continual importation of new rotavirus strains into southern Vietnam and report the emergence of rotavirus genotype G12

COMO É QUE OS ESTUDANTES DE MEDICINA E ENFERMAGEM VEEM A TECNOLOGIA DE SAÚDE? UM ESTUDO DE VALIDAÇÃO PSICOMÉTRICA DO QUESTIONÁRIO DE AVALIAÇÃO DE USABILIDADE EM BANGLADESH

The modernization of healthcare delivery is a reality in various international settings. To ensure efficient and safe use of the diverse forms of healthcare technology available, professionals and students must be receptive to incorporating such tools into their practice. Currently, there is no instrument in Bangladesh to assess healthcare students’ technology acceptance. Objective: To translate, culturally adapt, and validate the Usability Evaluation Questionnaire (UtEQ) among Bangladeshi healthcare students. Method: A cross-sectional study with a methodological approach was conducted in two phases. The first phase involved the translation of the UtEQ questionnaire to Bengali, following the six stages proposed by Beaton et al. In the second phase, the psychometric properties of the questionnaire were evaluated using a non-probability sample of 486 undergraduate healthcare students from three higher education institutions in Bangladesh. Confirmatory factor analysis was performed, and the Cronbach’s alpha coefficient was estimated to find out the internal consistency. Results: Internal consistency was found to be excellent for all scale dimensions, ranging from 0.88 to 0.92, while confirmatory factor analysis showed adequate goodness-of-fit indicators. Conclusion: The UtEQ-B provides a reliable and valid method for healthcare educators and researchers to assess technology acceptance among healthcare students during clinical training in Bangladesh.A modernização da prestação de cuidados de saúde é uma realidade em vários contextos internacionais. Para garantir a utilização eficiente e segura das diversas formas de tecnologia em saúde disponíveis, os profissionais e estudantes devem estar receptivos à incorporação dessas ferramentas na sua prática. Atualmente, não existe em Bangladesh um instrumento para avaliar a aceitação da tecnologia pelos estudantes de saúde. Objetivo: Traduzir, adaptar culturalmente e validar o Questionário de Avaliação da Usabilidade (UtEQ) entre estudantes de saúde de Bangladesh. Método: Foi realizado um estudo transversal com uma abordagem metodológica em duas fases. A primeira fase envolveu a tradução do questionário UtEQ para bengali, seguindo as seis etapas propostas por Beaton et al. Na segunda fase, foram avaliadas as propriedades psicométricas do questionário usando uma amostra não probabilística de 486 estudantes de graduação em saúde de três instituições de ensino superior em Bangladesh. Foi realizada uma análise fatorial confirmatória e estimou-se o coeficiente alfa de Cronbach para verificar a consistência interna. Resultados: Foi encontrada uma consistência interna excelente para todas as dimensões da escala, variando de 0,88 a 0,92, enquanto a análise fatorial confirmatória mostrou indicadores adequados de ajuste. Conclusão: O UtEQ-B fornece um método confiável e válido para educadores e pesquisadores em saúde avaliarem a aceitação da tecnologia entre estudantes de saúde durante o treinamento clínico em Bangladesh

ADAPTAÇÃO CULTURAL E VALIDAÇÃO PSICOMÉTRICA DO QUESTIONÁRIO SELF EFFICIENCY AND PERFORMANCE IN SELF-MANAGEMENT SUPPORT (SEPSS) EM ESTUDANTES DE GRADUAÇÃO EM ENFERMAGEM E MEDICINA DE BANGLADESH

In an aging society, healthcare professionals and students face increasing demands to actively involve patients in the decision-making process regarding their health conditions and lifestyles. Self-management support is considered a best practice that aligns with the patient-centered care paradigm in Bangladesh. However, there is currently no instrument available to assess healthcare professionals’ competencies in this field, particularly during their early education and training period. The aim of this study was to translate the Self Efficiency and Performance in Self-management Support (SEPSS) instrument into Bangla and validate its psychometric properties in a sample of undergraduate healthcare students in Bangladeshi higher education institutions. A cross-sectional study was conducted to assess the reliability, validity, and cultural appropriateness of the Bangla version of SEPSS-36 among 486 nursing and medical students. Confirmatory factor analysis was carried out using the chi-square model fit index (CMIN), comparative fit index (CFI), and Root Mean Square Error of Approximation (RMSEA) as fit indices. The internal consistency was estimated by the Cronbach alpha coefficient. The results indicate that the CMIN (2.658) and RMSEA (.058) values suggest that the sample data and hypothetical model are an acceptable fit in the analysis, with satisfactory CFI values (.895). The reliability for all SEPSS dimensions was acceptable. The Bangla version of the SEPSS questionnaire is a valid and reliable instrument that can assist healthcare educators and researchers in determining students’ competencies within this domain.Numa sociedade envelhecida, os profissionais de saúde e os estudantes enfrentam exigências cada vez maiores para envolver ativamente os pacientes no processo de tomada de decisão em relação às suas condições de saúde e estilos de vida. O apoio à autogestão é considerado uma prática recomendada que está alinhada com o paradigma de cuidados centrados no paciente em Bangladesh. No entanto, atualmente não existe um instrumento disponível para avaliar as competências dos profissionais de saúde nesse campo, especialmente durante o período inicial de educação e formação. O objetivo deste estudo foi traduzir o instrumento Self Efficiency and Performance in Self-management Support (SEPSS) para o bengali e validar as suas propriedades psicométricas numa amostra de estudantes de saúde de graduação em instituições de ensino superior de Bangladesh. Foi realizado um estudo transversal para avaliar a confiabilidade, validade e adequação cultural da versão em bengali do SEPSS-36 entre 486 estudantes de enfermagem e medicina. A análise fatorial confirmatória foi conduzida utilizando o índice de ajustamento do modelo qui-quadrado (CMIN), o índice de ajustamento comparativo (CFI) e o erro quadrado médio de aproximação (RMSEA) como índices de ajustamento. A consistência interna foi estimada pelo coeficiente alfa de Cronbach. Os resultados indicam que os valores de CMIN (2,658) e RMSEA (0,058) sugerem que os dados da amostra e o modelo hipotético têm um ajustamento aceitável na análise, com valores de CFI satisfatórios (0,895). A confiabilidade de todas as dimensões do SEPSS foi aceitável. A versão em bengali do questionário SEPSS é um instrumento válido e fiável que pode ajudar os educadores e investigadores em saúde a determinar as competências dos estudantes nesta área