Transitions and New Beginnings (2019-2020)

n her critical evaluation, Toy focuses on the progress she has made in developing her writing process as a college-level writer. Rather than relying solely on personal experience, Toy balances her essay with an analysis of her own experiences and first-person perspective and the use of several sources on writing and the writing process, including the textbook They Say/I Say. She uses her sources overall to support the claims and observations that she is bringing to her own writing, while she is talking about having learned that writing skill.https://digitalcommons.cortland.edu/rhetdragonscriticaleval/1001/thumbnail.jp

A Study on Anti-Maskers

In-depth interviews were conducted in September of 2021 with 15 Florida residents between the ages of 18-30 (8 males, 7 females). Our primary goal was to identify why these individuals choose not to wear face masks when required or highly recommended despite the potential legal, social, and health consequences. The researchers discovered that these individuals frame not wearing masks as physically harmless, and socially and legally acceptable. Specifically, these individuals justify their anti-masking actions using seven patterns arising from both their internal views and external influences. These patterns are: 1) Changing CDC mask wearing recommendations, 2) Mask wearing health consequences, 3) Denial of the severity of the virus, 4) Right to choose, 5) Florida living 6) Media influence, and 7) Others are not wearing masks. We discuss the limitations to the study and conclude with recommendations for those who are proud maskers in addition to a discussion providing further ideas for study

Intelligent Knee Sleeves: A Real-time Multimodal Dataset for 3D Lower Body Motion Estimation Using Smart Textile

The kinematics of human movements and locomotion are closely linked to the activation and contractions of muscles. To investigate this, we present a multimodal dataset with benchmarks collected using a novel pair of Intelligent Knee Sleeves (Texavie MarsWear Knee Sleeves) for human pose estimation. Our system utilizes synchronized datasets that comprise time-series data from the Knee Sleeves and the corresponding ground truth labels from the visualized motion capture camera system. We employ these to generate 3D human models solely based on the wearable data of individuals performing different activities. We demonstrate the effectiveness of this camera-free system and machine learning algorithms in the assessment of various movements and exercises, including extension to unseen exercises and individuals. The results show an average error of 7.21 degrees across all eight lower body joints when compared to the ground truth, indicating the effectiveness and reliability of the Knee Sleeve system for the prediction of different lower body joints beyond the knees. The results enable human pose estimation in a seamless manner without being limited by visual occlusion or the field of view of cameras. Our results show the potential of multimodal wearable sensing in a variety of applications from home fitness to sports, healthcare, and physical rehabilitation focusing on pose and movement estimation.Comment: Accepted by Thirty-seventh Conference on Neural Information Processing Systems (Neurips) D&B Trac

Discovery of diverse and functional antibodies from large human repertoire antibody libraries

AbstractPhage display antibody libraries have a proven track record for the discovery of therapeutic human antibodies, increasing the demand for large and diverse phage antibody libraries for the discovery of new therapeutics. We have constructed naïve antibody phage display libraries in both Fab and scFv formats, with each library having more than 250billion clones that encompass the human antibody repertoire. These libraries show high fidelity in open reading frame and expression percentages, and their V-gene family distribution, VH-CDR3 length and amino acid usage mirror the natural diversity of human antibodies. Both the Fab and scFv libraries show robust sequence diversity in target-specific binders and differential V-gene usage for each target tested, supporting the use of libraries that utilize multiple display formats and V-gene utilization to maximize antibody-binding diversity. For each of the targets, clones with picomolar affinities were identified from at least one of the libraries and for the two targets assessed for activity, functional antibodies were identified from both libraries

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

American Religious Sounds Project Site Coordinator Manual

The American Religious Sounds Project (ARSP) Site Coordinator Manual was compiled in 2019 to assist researchers and their students in following the ARSP procedures for site selection, recording, and archiving sounds. The topics covered in the ARSP manual include: project summary and history; site selection and fieldwork, using recording equipment, archiving and metadata, audio production, ethical guidelines, glossary of terms, and sample forms