Barrières pré-zygotiques chez les hybrides entre formes sauvages du niébé, Vigna unguilata (L.) Walp.

Hybrids pre-zygotic barriers between wild forms of cowpea. The wild forms of cowpea, Vigna unguiculata, constitute an important gene pool insufficiently exploited for the improvement of the cultivated form. In order to promote the use of these wild forms in the genetic improvement programmes, we undertook to understand the various incompatibility reactions which appear in the crosses between wild forms. Efforts were concentrated to understand the incompatibility barriers in the hybridizations between subsp. baoulensis NI 933 and the other wild forms of V. unguiculata. Thanks to the use of the aniline blue fluorescence, we observed a high frequency of pre-zygotic barriers. They appear in three sites, i.e. the higher and lower third of the style, and within the ovary. However, these incompatibility barriers are not absolute. Indeed, in our hybridizations, more than 4% of the ovules were fertilized in the various studied combinations

Analysis of incompatibility barriers during cross-pollinations among Phaseolus L. and other leguminous plants

Plant breeding is intended to seek for ways of realizing a genetic structure adapted to criteria and population needs. In this context, cross-pollinations are for great interest. They offer the means of creating a wide genotypic variability. However, a limited number of species are able to undergo hybridization studies. In Phaseolus L., improvement can be envisioned by either exploiting the primary genetic pool of the species, or the genetic variability of other species. In fact, there are wild or wild-like species more resistant to stresses or diseases than the cultivated ones. The methods of embryo rescues facilitate the interspecific crosses and contribute to exploiting the available genetic diversity within plant germplasm. Difficulties related to obtaining hybrids in grain legumes, and Phaseolus L. in particular, are reported in this research. It is possible to avoid the incompatibility barriers based on their appearance time. When they are pre-zygotic, the use of growth regulators during pollination is recommended. The in vitro explants culture is the most usual method to overcome the post-zygotic barriers. The mechanisms leading to abortion of hybrid embryos, as well as adequate methods to overcome the interspecific incompatibilities should be subjected to further studies.Keywords: Abortion, hybridization, interspecific barriers, Phaseolus L., plant embryos

Amélioration du taux de multiplication in vitro de Jatropha curcas L

Improvement of the Rate of in vitro Multiplication of Jatropha curcas L. In order to improve the in vitro multiplication rate (number of shoots/explant/subculture) of Jatropha curcas L. axillary nodes taken from young plants of two accessions of this species (originating from Cameroon and Senegal) have been cultivated for three weeks on a MS medium supplemented with 8.87 μM BAP, 4.92 μM IBA, and 30 g/l sucrose at pH 5.7 ± 0.1, and solidified with 0.7% agar. The shoots obtained from each original explant were then transferred to proliferation media (PM) consisting of MS medium supplemented with 2.21 to 8.9 μM BA or 2.21 to 8.9 μM kinetin in combination with 2.46 μM IBA. Each combination was completed with 33.12 μM adenine sulfate, 82.92 μM of glutamine and 30 g / L sucrose. The best multiplication rate was obtained for the PM medium containing 6.65 μM BA and 2.46 μM AIB. On this medium 42.72 ± 3.22 and 38.15 ± 4.7 shoots/explant were obtained respectively for the accessions from Cameroon and Senegal after 6 weeks of culture, and the mean multiplication rates were 8.27 ± 1.27 (accession from Cameroon) and 7.89 ± 1.13 (accession from Senegal) shoots per explant during the 7 following subcultures (3 weeks/subculture). This medium was also the one that allowed the best overall growth in shoot height. Leafy shoots obtained have been rooted in a medium containing half of the major mineral components of MS supplemented with 5.7 μM IBA, 1.5% sucrose and solidified with 0.7% agar, then acclimated with a survival rate of 97%. These results allow considering the establishment of industrial units of plantlet multiplication from elite clones of Jatropha curcas

Les hybridations interspecifiques dans le genre Phaseolus : selection des genotypes compatibles et caracterisation des hybrides interspecifiques

La réussite de l’introgression de caractères utiles chez le haricot commun P. vulgaris à partir des deux espèces P. coccineus et P. polyanthus dépend en partie des génotypes utilisés. Ce travail vise à identifier des génotypes de Phaseolus compatibles lors des hybridations interspécifiques et à identifier les hybrides issus des croisements entre P. vulgaris utilisé comme parent mâle et les deux autres espèces utilisées comme parents femelles. Dans les croisements P. coccineus x P. vulgaris, le taux de gousses avortées audelà de 8 JAP est d’environ 12 %. Quatre gousses supposées hybrides ont été obtenues. Pour les hybridations P. polyanthus x P. vulgaris, le taux de gousses avortés au-delà de 8 JAP est d’environ 18 % et une seule gousse supposée hybride a été produite. Les hybrides issus des deux combinaisons interspécifiques ont été mis en évidence au moyen de caractères morphologiques et grâce à un marqueur moléculaire microsatellite. Les génotypes retenus pour leur bonne aptitude à la combinaison, en tenant compte des taux d’avortement au-delà de 8 JAP, sont les suivants : NI637 et G21245 pour P. vulgaris, NI889 et NI16 pour P. coccineus, NI1015 et G35348 pour P. polyanthus. Mots clés : Phaseolus, hybridations interspécifiques, caractères morphologiques, marqueur microsatellite (SSR).INTERSPECIFIC HYBRIDIZATIONSWITHIN THE GENUS PhaseolusSuccessful introgression of useful traits in common bean P. vulgaris from the two species P. coccineus and P. polyanthus depends in part to genotypes used. This work aims to identify genotypes of Phaseolus compatible during interspecific crosses and to identify hybrids from crosses between P. vulgaris used as male parent and the other two species used as female parents. In crosses P. coccineus x P. vulgaris, the rate of pod abortion 8 days after pollination (DAP) is around 12 %. Four putative hybrid pods were obtained. For hybridizations P. polyanthus x P. vulgaris, the rate of pod abortion 8 DAP is around 18 % and one putative hybrid pod was produced. The hybrid nature of one plant from each interspecific combination was confirmed using morphological characters and one microsatellite molecular marker. Genotypes selected for good combining abilities, considering pod abortion rates 8 DAP are NI637 and G21245 for P. vulgaris, NI889 and NI16 for P. coccineus, NI1015 and G35348 for P. polyanthus.Keywords : Phaseolus, interspecific hybridizations, morphological characters, microsatellite marker (SSR)

Decay of Correlations in a Topological Glass

In this paper we continue the study of a topological glassy system. The state space of the model is given by all triangulations of a sphere with $N$ nodes, half of which are red and half are blue. Red nodes want to have 5 neighbors while blue ones want 7. Energies of nodes with other numbers of neighbors are supposed to be positive. The dynamics is that of flipping the diagonal between two adjacent triangles, with a temperature dependent probability. We consider the system at very low temperatures. We concentrate on several new aspects of this model: Starting from a detailed description of the stationary state, we conclude that pairs of defects (nodes with the "wrong" degree) move with very high mobility along 1-dimensional paths. As they wander around, they encounter single defects, which they then move "sideways" with a geometrically defined probability. This induces a diffusive motion of the single defects. If they meet, they annihilate, lowering the energy of the system. We both estimate the decay of energy to equilibrium, as well as the correlations. In particular, we find a decay like $t^{-0.4}$

Variability in spine radiosurgery treatment planning - results of an international multi-institutional study

Background: The aim of this study was to quantify the variability in spinal radiosurgery (SRS) planning practices between five international institutions, all member of the Elekta Spine Radiosurgery Research Consortium. Methods: Four institutions provided one representative patient case each consisting of the medical history, CT and MR imaging. A step-wise planning approach was used where, after each planning step a consensus was generated that formed the basis for the next planning step. This allowed independent analysis of all planning steps of CT-MR image registration, GTV definition, CTV definition, PTV definition and SRS treatment planning. In addition, each institution generated one additional SRS plan for each case based on intra-institutional image registration and contouring, independent of consensus results. Results: Averaged over the four cases, image registration variability ranged between translational 1.1 mm and 2.4 mm and rotational 1.1° and 2.0° in all three directions. GTV delineation variability was 1.5 mm in axial and 1.6 mm in longitudinal direction averaged for the four cases. CTV delineation variability was 0.8 mm in axial and 1.2 mm in longitudinal direction. CTV-to-PTV margins ranged between 0 mm and 2 mm according to institutional protocol. Delineation variability was 1 mm in axial directions for the spinal cord. Average PTV coverage for a single fraction18 Gy prescription was 87 ± 5 %; Dmin to the PTV was 7.5 ± 1.8 Gy averaged over all cases and institutions. Average Dmax to the PRV_SC (spinal cord + 1 mm) was 10.5 ± 1.6 Gy and the average Paddick conformity index was 0.69 ± 0.06. Conclusions: Results of this study reflect the variability in current practice of spine radiosurgery in large and highly experienced academic centers. Despite close methodical agreement in the daily workflow, clinically significant variability in all steps of the treatment planning process was demonstrated. This may translate into differences in patient clinical outcome and highlights the need for consensus and established delineation and planning criteria

Hypericin and pseudohypericin concentrations of a valuable medicinal plant Hypericum perforatum L. are enhanced by arbuscular mycorrhizal fungi

Hypericum perforatum L. (St. John’s-wort, Hypericaceae) is a valuable medicinal plant species cultivated for pharmaceutical purposes. Although the chemical composition and pharmacological activities of H. perforatum have been well studied, no data are available concerning the influence of arbuscular mycorrhizal fungi (AMF) on this important herb. A laboratory experiment was therefore conducted in order to test three AMF inocula on H. perforatum with a view to show whether AMF could influence plant vitality (biomass and photosynthetic activity) and the production of the most valuable secondary metabolites, namely anthraquinone derivatives (hypericin and pseudohypericin) as well as the prenylated phloroglucinol—hyperforin. The following treatments were prepared: (1) control—sterile soil without AMF inoculation, (2) Rhizophagus intraradices (syn. Glomus intraradices), (3) Funneliformis mosseae (syn. Glomus mosseae), and (4) an AMF Mix which contained: Funneliformis constrictum (syn. Glomus constrictum), Funneliformis geosporum (syn. Glomus geosporum), F. mosseae, and R. intraradices. The application of R. intraradices inoculum resulted in the highest mycorrhizal colonization, whereas the lowest values of mycorrhizal parameters were detected in the AMF Mix. There were no statistically significant differences in H. perforatum shoot mass in any of the treatments. However, we found AMF species specificity in the stimulation of H. perforatum photosynthetic activity and the production of secondary metabolites. Inoculation with the AMF Mix resulted in higher photosynthetic performance index (PItotal) values in comparison to all the other treatments. The plants inoculated with R. intraradices and the AMF Mix were characterized by a higher concentration of hypericin and pseudohypericin in the shoots. However, no differences in the content of these metabolites were detected after the application of F. mosseae. In the case of hyperforin, no significant differences were found between the control plants and those inoculated with any of the AMF applied. The enhanced content of anthraquinone derivatives and, at the same time, better plant vitality suggest that the improved production of these metabolites was a result of the positive effect of the applied AMF strains on H. perforatum. This could be due to improved mineral nutrition or to AMF-induced changes in the phytohormonal balance. Our results are promising from the biotechnological point of view, i.e. the future inoculation of H. perforatum with AMF in order to improve the quality of medicinal plant raw material obtained from cultivation

A prognostic index predicting survival in transformed Waldenström macroglobulinemia

Histological transformation into diffuse large B-cell lymphoma is a rare complication in patients with Waldenström macroglobulinemia (WM) usually associated with a poor prognosis. The objective of this study was to develop and validate a prognostic index for survival in transformed WM patients. Through this multicenter, international collaborative effort, we developed a scoring system based on data from 133 patients with transformed WM who were evaluated between 1995 and 2016 (training cohort). Univariate and multivariate analyses were used to propose a prognostic index with 2-year survival after transformation as an end-point. For external validation, a data set of 67 patients was used to evaluate the performance of the model (validation cohort). By multivariate analysis, three adverse covariates were identified as independent predictors of 2-year survival after transformation: elevated serum LDH (2 points), platelet count < 100 x 109/L (1 point) and any previous treatment for WM (1 point). Three risk groups were defined: low-risk (0-1 point, 24% of patients), intermediate-risk (2-3 points, 59%, hazard ratio (HR) = 3.4) and high-risk (4 points, 17%, HR = 7.5). Two-year survival rates were 81%, 47%, and 21%, respectively (P < 0.0001). This model appeared to be a better discriminant than the International Prognostic Index (IPI) and the revised IPI (R-IPI). We validated this model in an independent cohort. This easy-to-compute scoring index is a robust tool that may allow identification of groups of transformed WM patients with different outcomes and could be used for improving the development of risk-adapted treatment strategies

Individual-environment interactions in swimming: The smallest unit for analysing the emergence of coordination dynamics in performance?

Displacement in competitive swimming is highly dependent on fluid characteristics, since athletes use these properties to propel themselves. It is essential for sport scientists and practitioners to clearly identify the interactions that emerge between each individual swimmer and properties of an aquatic environment. Traditionally, the two protagonists in these interactions have been studied separately. Determining the impact of each swimmer’s movements on fluid flow, and vice versa, is a major challenge. Classic biomechanical research approaches have focused on swimmers’ actions, decomposing stroke characteristics for analysis, without exploring perturbations to fluid flows. Conversely, fluid mechanics research has sought to record fluid behaviours, isolated from the constraints of competitive swimming environments (e.g. analyses in two-dimensions, fluid flows passively studied on mannequins or robot effectors). With improvements in technology, however, recent investigations have focused on the emergent circular couplings between swimmers’ movements and fluid dynamics. Here, we provide insights into concepts and tools that can explain these on-going dynamical interactions in competitive swimming within the theoretical framework of ecological dynamics

Low CD10 mRNA Expression Identifies High-Risk Ductal Carcinoma In Situ (DCIS)

PURPOSE: Optimal management of breast ductal carcinoma in situ (DCIS) is controversial, and many patients are still overtreated. The local death of myoepithelial cells (MECs) is believed to be a pre-requisite to tumor invasion. We thus hypothesized that loss of CD10 expression, a MEC surface peptidase, would signify basement membrane disruption and confer increased risk of relapse in DCIS. The aim of our study was to retrospectively evaluate the prognostic value of CD10 in DCIS. EXPERIMENTAL DESIGN: CD10 expression was evaluated by quantitative RT-PCR and immunohistochemistry using paraffin-embedded samples of normal breast tissue (n = 11); of morphologically normal ducts associated with DCIS (n = 10); and of DCIS without an invasive component (n = 154). RESULTS: CD10 immunostaining was only observed in MECs in normal tissue and in DCIS. Normal tissue showed high mRNA expression levels of CD10, whereas DCIS showed a variable range. After a median follow-up of 6 years, DCIS with CD10 expression below the levels observed in normal tissue (71%) demonstrated a higher risk of local relapse (HR = 1.88; [95CI:1.30-2.70], p = 0.001) in univariate analysis. No relapse was observed in patients expressing high CD10 mRNA levels (29%) similar to the ones observed in normal tissue. In multivariate analysis including known prognostic factors, low CD10 mRNA expression remained significant (HR = 2.25; [95%CI:1.24-4.09], p = 0.008), as did the recently revised Van Nuys Prognostic Index (VNPI) score (HR = 2.03; [95%CI:1.23-3.35], p = 0.006). CONCLUSION: The decrease of CD10 expression in MECs is associated with a higher risk of relapse in DCIS; this knowledge has the potential to improve DCIS management