The platelet ATP and ADP receptors.

peer reviewedThis review focuses on recent findings on the physiology of these platelet ADP and ATP receptors, their distinct downstream intracellular signaling pathways as well as on the available agonists, antagonists and inhibitors that allow their pharmacological discrimination

THE EFFECT OF DAILY PHYSICAL EDUCATION LESSONS ON HUNGARIAN STUDENT'S PHYSICAL ACTIVITY LEVELS AND BODY COMPOSITION

Lifestyle, physical activity level and nutritional habits as well as environmental factors are having a greater and greater detrimental effect on the health of various populations. In the present study we systematically researched the physical status of schoolchildren. It is well known that as children grow up, the amount of spontaneous physical activity they perform decreases as they have more mental tasks to do these negative factors can be offset with daily physical education. We measured children participating in physical education on a daily basis, consisting of 5th (10-11 years old) and 9th (14-15 years old) grade students and control groups of 4th (9-10 years old) and 8th (13-14 years old) grade students, respectively, for a total of 196 persons (94 girls and 102 boys). Habitual physical activity was obtained with Actigraph, (GT1M/GT3X) and body fat percentage was calculated from anthropometrical measurements. As expected, we were able to observe a linear correlation between the physical activity levels and the body composition of the participants. The more time the subjects spent performing MVPA (Moderate and Vigorous Physical Activity), the less body fat they had. The recommended MVPA is a minimum of one hour per day, but these Hungarian children had two hours of MPVA per day. Our results lead us to conclude that ninety minutes of additional physical education per week for 7-8 months cannot be considered sufficient for a full impact analysis nor for schoolchildren’s physical activity needs or body composition, though an increase in the amount of time spent in physical education classes shows beneficial effects for these children

THE EFFECT OF DAILY PHYSICAL EDUCATION LESSONS ON HUNGARIAN STUDENT'S PHYSICAL ACTIVITY LEVELS AND BODY COMPOSITION

Lifestyle, physical activity level and nutritional habits as well as environmental factors are having a greater and greater detrimental effect on the health of various populations. In the present study we systematically researched the physical status of schoolchildren. It is well known that as children grow up, the amount of spontaneous physical activity they perform decreases as they have more mental tasks to do these negative factors can be offset with daily physical education. We measured children participating in physical education on a daily basis, consisting of 5th (10-11 years old) and 9th (14-15 years old) grade students and control groups of 4th (9-10 years old) and 8th (13-14 years old) grade students, respectively, for a total of 196 persons (94 girls and 102 boys). Habitual physical activity was obtained with Actigraph, (GT1M/GT3X) and body fat percentage was calculated from anthropometrical measurements. As expected, we were able to observe a linear correlation between the physical activity levels and the body composition of the participants. The more time the subjects spent performing MVPA (Moderate and Vigorous Physical Activity), the less body fat they had. The recommended MVPA is a minimum of one hour per day, but these Hungarian children had two hours of MPVA per day. Our results lead us to conclude that ninety minutes of additional physical education per week for 7-8 months cannot be considered sufficient for a full impact analysis nor for schoolchildren’s physical activity needs or body composition, though an increase in the amount of time spent in physical education classes shows beneficial effects for these children

Bidirectional Relationship Between Reduced Blood pH and Acute Pancreatitis: A Translational Study of Their Noxious Combination

Acute pancreatitis (AP) is often accompanied by alterations in the acid-base balance, but how blood pH influences the outcome of AP is largely unknown. We studied the association between blood pH and the outcome of AP with meta-analysis of clinical trials, and aimed to discover the causative relationship between blood pH and AP in animal models. PubMed, EMBASE, and Cochrane Controlled Trials Registry databases were searched from inception to January 2017. Human studies reporting systemic pH status and outcomes (mortality rate, severity scores, and length of hospital stay) of patient groups with AP were included in the analyses. We developed a new mouse model of chronic metabolic acidosis (MA) and induced mild or severe AP in the mice. Besides laboratory blood testing, the extent of pancreatic edema, necrosis, and leukocyte infiltration were assessed in tissue sections of the mice. Thirteen studies reported sufficient data in patient groups with AP (n = 2,311). Meta-analysis revealed markedly higher mortality, elevated severity scores, and longer hospital stay in AP patients with lower blood pH or base excess (P < 0.001 for all studied outcomes). Meta-regression analysis showed significant negative correlation between blood pH and mortality in severe AP. In our mouse model, pre-existing MA deteriorated the pancreatic damage in mild and severe AP and, vice versa, severe AP further decreased the blood pH of mice with MA. In conclusion, MA worsens the outcome of AP, while severe AP augments the decrease of blood pH. The discovery of this vicious metabolic cycle opens up new therapeutic possibilities in AP

Does the P(2X1del) variant lacking 17 amino acids in its extracellular domain represent a relevant functional ion channel in platelets?

In this letter, we questionned the existence of a ADP responsive P2X1 protein variant in platelets

Evidence for diagnosis of early chronic pancreatitis after three episodes of acute pancreatitis : a cross-sectional multicentre international study with experimental animal model

Chronic pancreatitis (CP) is an end-stage disease with no specific therapy; therefore, an early diagnosis is of crucial importance. In this study, data from 1315 and 318 patients were analysed from acute pancreatitis (AP) and CP registries, respectively. The population from the AP registry was divided into AP (n=983), recurrent AP (RAP, n=270) and CP (n=62) groups. The prevalence of CP in combination with AP, RAP2, RAP3, RAP4 and RAP5+was 0%, 1%, 16%, 50% and 47%, respectively, suggesting that three or more episodes of AP is a strong risk factor for CP. Laboratory, imaging and clinical biomarkers highlighted that patients with RAP3+do not show a significant difference between RAPs and CP. Data from CP registries showed 98% of patients had at least one AP and the average number of episodes was four. We mimicked the human RAPs in a mouse model and found that three or more episodes of AP cause early chronic-like morphological changes in the pancreas. We concluded that three or more attacks of AP with no morphological changes to the pancreas could be considered as early CP (ECP).The new diagnostic criteria for ECP allow the majority of CP patients to be diagnosed earlier. They can be used in hospitals with no additional costs in healthcare.Peer reviewe

Representativeness of microsatellite distributions in genomes, as revealed by 454 GS-FLX Titanium pyrosequencing

<p>Abstract</p> <p>Background</p> <p>Microsatellites are markers of choice in population genetics and genomics, as they provide useful insight into patterns and processes as diverse as genome evolutionary dynamics and demographic processes. The acquisition of microsatellites through multiplex-enriched libraries and 454 GS-FLX Titanium pyrosequencing is a promising new tool for the isolation of new markers in unknown genomes. This approach can also be used to evaluate the extent to which microsatellite-enriched libraries are representative of the genome from which they were isolated. In this study, we deciphered potential discrepancies in microsatellite content recovery for two reference genomes (<it>Apis mellifera </it>and <it>Danio rerio</it>), selected on the basis of their extreme heterogeneity in terms of the proportions and distributions of microsatellites on chromosomes.</p> <p>Results</p> <p>The <it>A. mellifera </it>genome, in particular, was found to be highly heterogeneous, due to extremely high rates of recombination, with hotspots, but the only bias consistently introduced into pyrosequenced multiplex-enriched libraries concerned sequence length, with the overrepresentation of sequences 160 to 320 bp in length. Other deviations from expected proportions or distributions of motifs on chromosomes were observed, but the significance and intensity of these deviations was mostly limited. Furthermore, no consistent adverse competition between multiplexed probes was observed during the motif enrichment phase.</p> <p>Conclusions</p> <p>This approach therefore appears to be a promising strategy for improving the development of microsatellites, as it introduces no major bias in terms of the proportions and distribution of microsatellites.</p