Cytochrome oxidase subunit VI of Trypanosoma brucei is imported without a cleaved presequence and is developmentally regulated at both RNA and protein levels

Mitochondrial respiration in the African trypanosome undergoes dramatic developmental stage regulation. This requires co-ordinated control of components encoded by both the nuclear genome and the kinetoplast, the unusual mitochondrial genome of these parasites. As a model for understanding the co-ordination of these genomes, we have examined the regulation and mitochondrial import of a nuclear-encoded component of the cytochrome oxidase complex, cytochrome oxidase subunit VI (COXVI). By generating transgenic trypanosomes expressing intact or mutant forms of this protein, we demonstrate that COXVI is not imported using a conventional cleaved presequence and show that sequences at the N-terminus of the protein are necessary for correct mitochondrial sorting. Analyses of endogenous and transgenic COXVI mRNA and protein expression in parasites undergoing developmental stage differentiation demonstrates a temporal order of control involving regulation in the abundance of, first, mRNA and then protein. This represents the first dissection of the regulation and import of a nuclear-encoded protein into the cytochrome oxidase complex in these organisms, which were among the earliest eukaryotes to possess a mitochondrion

Evidence for a mixed mass composition at the `ankle' in the cosmic-ray spectrum

We report a first measurement for ultra-high energy cosmic rays of the correlation between the depth of shower maximum and the signal in the water Cherenkov stations of air-showers registered simultaneously by the fluorescence and the surface detectors of the Pierre Auger Observatory. Such a correlation measurement is a unique feature of a hybrid air-shower observatory with sensitivity to both the electromagnetic and muonic components. It allows an accurate determination of the spread of primary masses in the cosmic-ray flux. Up till now, constraints on the spread of primary masses have been dominated by systematic uncertainties. The present correlation measurement is not affected by systematics in the measurement of the depth of shower maximum or the signal in the water Cherenkov stations. The analysis relies on general characteristics of air showers and is thus robust also with respect to uncertainties in hadronic event generators. The observed correlation in the energy range around the `ankle' at $\lg(E/{\rm eV})=18.5-19.0$ differs significantly from expectations for pure primary cosmic-ray compositions. A light composition made up of proton and helium only is equally inconsistent with observations. The data are explained well by a mixed composition including nuclei with mass $A > 4$. Scenarios such as the proton dip model, with almost pure compositions, are thus disfavoured as the sole explanation of the ultrahigh-energy cosmic-ray flux at Earth.Comment: Published version. Added journal reference and DOI. Added Report Numbe

The Pierre Auger Observatory Status And Latest Results

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Astrophysical Interpretation Of Pierre Auger Observatory Measurements Of The Uhecr Energy Spectrum And Mass Composition

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Evidence For A Mixed Mass Composition At The ‘ankle’ In The Cosmic-ray Spectrum

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Evidence for a mixed mass composition at the ‘ankle’ in the cosmic-ray spectrum

We report a first measurement for ultrahigh energy cosmic rays of the correlation between the depth of shower maximum and the signal in the water Cherenkov stations of air-showers registered simultaneously by the fluorescence and the surface detectors of the Pierre Auger Observatory. Such a correlation measurement is a unique feature of a hybrid air-shower observatory with sensitivity to both the electromagnetic and muonic components. It allows an accurate determination of the spread of primary masses in the cosmic-ray flux. Up till now, constraints on the spread of primary masses have been dominated by systematic uncertainties. The present correlation measurement is not affected by systematics in the measurement of the depth of shower maximum or the signal in the water Cherenkov stations. The analysis relies on general characteristics of air showers and is thus robust also with respect to uncertainties in hadronic event generators. The observed correlation in the energy range around the \u2018ankle\u2019 at lg\u2061(E/eV)=18.5\u201319.0 differs significantly from expectations for pure primary cosmic-ray compositions. A light composition made up of proton and helium only is equally inconsistent with observations. The data are explained well by a mixed composition including nuclei with mass A>4. Scenarios such as the proton dip model, with almost pure compositions, are thus disfavored as the sole explanation of the ultrahigh-energy cosmic-ray flux at Earth

The Pierre Auger Observatory status and latest results

The Pierre Auger Observatory, in Argentina, is the present flagship experiment studying ultrahigh-energy cosmic rays (UHECRs). Facing the challenge due to low cosmic-ray flux at the highest energies, the Observatory has been taking data for more than a decade, reaching an exposure of over 50 000 km2sr yr. The combination of a large surface detector array and fluorescence telescopes provides a substantial improvement in energy calibration and extensive air shower measurements, resulting in data of unprecedented quality. Moreover, the installation of a denser subarray has allowed extending the sensitivity to lower energies. Altogether, this contributes to provide important information on key questions in the UHECR field in the energy range from 0.1 EeV up to 100 EeV. A review of main results from the Pierre Auger Observatory is presented with a particular focus on the energy spectrum measurements, the mass composition studies, the arrival directions analyses, the search for neutral cosmic messengers, and the investigation of high-energy hadronic interactions. Despite this large amount of valuable results, the understanding of the nature of UHECRs and of their origin remains an open science case that the Auger collaboration is planning to address with the AugerPrime project to upgrade the Observatory

The Pierre Auger Observatory Upgrade

It is planned to operate the Pierre Auger Observatory until at least the end of 2024. An upgrade of the experiment has been proposed in order to provide additional measurements to allow one to elucidate the mass composition and the origin of the flux suppression at the highest energies, to search for a flux contribution of protons up to the highest energies and to reach a sensitivity to a contribution as small as 10% in the flux suppression region, to study extensive air showers and hadronic multi-particle production. With operation planned until 2024, event statistics will more than double compared with the existing Auger data set, with the critical added advantage that every event will now have mass information. Obtaining additional composition-sensitive information will not only help to better reconstruct the properties of the primary particles at the highest energies, but also improve the measurements in the energy range just above the ankle. Furthermore, measurements with the new detectors will help to reduce systematic uncertainties related to the modelling hadronic showers and to limitations in the reconstruction algorithms. A description of the principal proposed Auger upgrade will be presented. The Auger upgrade promises high-quality future data, and real scope for new physics

Astrophysical interpretation of Pierre Auger Observatory measurements of the UHECR energy spectrum and mass composition

We present a combined fit of a simple astrophysical model of UHECR sources to both the energy spectrum and mass composition data measured by the Pierre Auger Observatory. The fit has been performed for energies above 5 EeV, i.e. the region of the all-particle spectrum above the so-called "ankle"' feature. The astrophysical model we adopted consists of identical sources uniformly distributed in a comoving volume, where nuclei are accelerated with a rigidity-dependent mechanism. The fit results suggest sources characterized by relatively low maximum injection energies and hard spectral indices. The impact of various systematic uncertainties on the above result is discussed