Histological Investigation of Experimentally Induced Diabetes Effects on the Distribution of Transforming Growth Factor (TGF beta), Nuclear Factor Kappa B (Nf-kappa B), Heat Schock 90 beta (Hsp90 beta) and E-cadherin Proteins in Testicular Tissue

WOS:000609557500003Diabetes is a metabolic disorder characterized by high blood sugar levels and it causes complications in many systems, including the reproductive system. As a result of diabetic conditions, one of the mechanisms that can cause repression of reproductive activity is testicular oxidant stress. The identification of diabetes on the cell signaling molecules axis is still under discussion. The aim of this study was to detennine the effect of Transfonning Growth Factor (TGF beta), Nuclear Factor kappa B (NF-kappa B), Heat-schock 90 beta (HSP90 beta) signal pathways and E-cadherin cell adhesion molecule on infertility in diabetic rat testicular tissue. in our study, includes histological, molecular and biochemical analysis of testicular tissue removed at the end of the 2 weeks experiment period. A total of 14 adult male rats were divided as control and diabetes. No intervention was given to 7 male rats in the control group. For the diabetic group, 7 male rats were injected by intraperitoneal with a single dose of 55 mg/kg streptozotocin (STZ). TGF beta, NF-kappa B, HSP90 beta and E-cadherin proteins were immunohistochemically studied to investigate possible tissue damage, inflanunatoly process, cell stabilization and integrity due to diabetes. in order to determine oxidant stress, lipid peroxidation product malondialdehyde (MDA), glutathione (GSH) and glutathione peroxidase (GPx) analyzes were performed. Fibrosis, inflammatory changes and loss of spermatogenetic series are prominent findings in the diabetic group. on analysis of all the samples with immunostaining, in the diabetic group, TGF beta and NF-kappa B inununoexpression significantly increased, while Hsp90 beta and E-cadherin immunoexpression significantly decreased compared with control groups. Experimental diabetes was found to cause fibrosis, inflammation, disrupting cell adhesion and stabilization in testicular tissue. These results suggest that cellular therapy studies are needed for possible damage

The Effect of Herbal Medicine on Colon Cancer Cells in Culture

Medicinal herbs are being increasingly recognized as useful complementary treatments for cancer. Colon cancer is the third most common cancer in men and the second in women worldwide. Colon cancer diseases is very heterogeneous in terms of grade, genetics, ploidy, and oncogene/tumor suppress or gene expression and its biological, hormonal, and molecular characteristics are extremely complex. In this study our aim was to identify the effect of different medical plants Viscum album, Inula viscosa, Hypericum perforatum, Lysimachia nummularia, Oleocanthal, Pinus pinaster and Rubus caeisus on colon cancer cell line. Colo320 cancer cells and human adipose tissue derived mesenchymal stem cell were analysed for four medical plants in culture. Firstly, the cytotoxicity rate (IC50) determined by MTT. After that, immunocytochemical staining were done eNOS, VEGF and TUNEL method for apoptosis. The stainings were evaluated by H-score. As a result, Inula viscosa and Rubus caeisus have a higher inhibitory effect on cell proliferation and apoptosis in both cancers than the other medicinal plants. Colo320 cancer cells expressed strong eNOS by these plants and also both plants were not toxic for adipose tissue derived mesenchymal stem cells

The Effect of Herbal Medicine on Prostate Cancer Cells in Culture

Herbal medicine has been used historically for anticancer effect and potent antiproliferative effects on different cancer cell lines. Prostate cancer has become the most common malignancy and the second highest cause of cancer death Western world. The antioxidant, antidepressant and antimicrobial effect of Viscum album, Inula viscosa, Hypericum perforatum, Lysimachia nummularia, Oleocanthal, Pinus pinaster and Rubus caeisus on cancer cell lines. In this study, we investigated whether these seven plants show any anticancer properties on prostat cancer cell line. Du145 cancer cells and human adipose tissue derived mesenchymal stem cell were assessed for seven medical plants in culture with different doses. Firstly, the cytotoxicity rate (IC50) determined by MTT and then immunocytochemical procedure were done for oxidative stress (eNOS), angiogenesis (VEGF) and TUNEL method for apoptosis. The stainings were evaluated by H-score. According to the results, Inula viscosa and Rubus caeisus have a higher improvement effect than the other medicinal plants. And these plants were not toxic for adipose tissue derived mesenchymal stem cells. These plants caused the inhibition of cell proliferation and apoptosis in prostat cancer cells. Our results indicated that these plants may be potential agents in prostat cancer treatment

Predictors of Severe and Permanent Disability in Children Evaluated in Health Boards: A Single-Center Study

Aim: The aim of this study is to determine the severe and permanent disability of the childrenevaluated in the disabled health boards and to evaluate the predictors of severe and permanentdisability.Material and Methods: Records of 1482 children who were referred to a university hospitalhealth board for disability between the years 2013-2018 were screened retrospectively.Results: More than half (52.2%) of the children had a single psychopathology. 83.5% of thecases were severely disabled and 66.5% were permanently disabled. Severely disabled childrenwere significantly more likely to have psychiatric, pediatric, neurological, orthopedic andotorhinolaringologic disorders. Permanent disability was significantly more frequent amongpatients with pediatric, ophthalmologic and cardiac disorders while children withpsychopathologies were significantly less likely to have permanent disability. Mentalretardation/intellectual developmental disorder levels differed in rates of permanent disabilitywith pair-wise comparisons revealing that severe mental retardation/intellectualdevelopmental disorder was the main factor. Severe disability was significantly more commonamong children younger than 8 years while permanent disability was more common amongchildren >3 years. Children with psychopathology were 4.1 times more likely to have severedisability and this further increased to 15.3 for those with mental retardation/intellectualdevelopmental disorder.Conclusion: This is the first study to evaluate the factors that affecting the decisions ofpermanent and severe disabilities in disability health boards. Comprehensive results have beenachieved despite low generalizability. To provide consistent reports, further and multicenterstudies on factors associated with severe and permanent disabilities in children are needed

Predictors of Severe and Permanent Disability in Children Evaluated in Health Boards: A Single-Center Study

Aim: The aim of this study is to determine the severe and permanent disability of the childrenevaluated in the disabled health boards and to evaluate the predictors of severe and permanentdisability.Material and Methods: Records of 1482 children who were referred to a university hospitalhealth board for disability between the years 2013-2018 were screened retrospectively.Results: More than half (52.2%) of the children had a single psychopathology. 83.5% of thecases were severely disabled and 66.5% were permanently disabled. Severely disabled childrenwere significantly more likely to have psychiatric, pediatric, neurological, orthopedic andotorhinolaringologic disorders. Permanent disability was significantly more frequent amongpatients with pediatric, ophthalmologic and cardiac disorders while children withpsychopathologies were significantly less likely to have permanent disability. Mentalretardation/intellectual developmental disorder levels differed in rates of permanent disabilitywith pair-wise comparisons revealing that severe mental retardation/intellectualdevelopmental disorder was the main factor. Severe disability was significantly more commonamong children younger than 8 years while permanent disability was more common amongchildren >3 years. Children with psychopathology were 4.1 times more likely to have severedisability and this further increased to 15.3 for those with mental retardation/intellectualdevelopmental disorder.Conclusion: This is the first study to evaluate the factors that affecting the decisions ofpermanent and severe disabilities in disability health boards. Comprehensive results have beenachieved despite low generalizability. To provide consistent reports, further and multicenterstudies on factors associated with severe and permanent disabilities in children are needed

Antiproliferative and Apoptotic Effects of the Medicinal Plants on Breast Cancer Cell Lines

Inula viscosa, Rubus caesius and Viscum album known as medicinal plants which have therapeutic properties in treatment of various diseases and particularly cancer. In this study, we aimed to show their anti-proliferative and apoptotic effects on MCF-7, MDA-MB-231, 67NR and 4T1 breast cancer cell lines. The cell lines were cultured to determine the cell growth using the MTT assay. Cells were cultured to evaluate for eNOS, VEGF and apoptosis by immunocytochemical staining and TUNEL assay. Statistical analysis was performed with the H-score. Inula viscosa, Rubus caesius and Viscum album extracts were found to significantly inhibit the growth of breast cancer cell lines in dose and time-dependent manner. eNOS staining was significantly increased and VEGF was decreased after extract application at IC50 dose. Similarly, results indicated that the treated breast cancer cell lines exhibited a marked increase in apoptosis. We showed that the toxic effect of Inula viscosa, Rubus caesius and Viscum album extracts created by oxidative stress and apoptotic mechanisms. Our findings suggested that due to their anticancer potential, these plants may be used as an alternative treatment for the management of cancer. Therefore future studies will focus on the identification of the molecules responsible for the anticancer activity

functional outcome in late adolescence/early adulthood of patients with autism spectrum disorder and its relationships with parental burnout and depression: A preliminary multi-center, cross-sectional study.

The aim of this study is to determine the functioning of adults with autism spectrum disorders (ASDs) diagnosed in childhood and depression and burnout levels among their parents. A total of 261 adults with ASDs and their parents were recruited for the study. Both parents completed the Beck Depression and Maslach Burnout Inventories and reported the functioning of their adult offspring with ASDs. Only 5.4 % of our sample reported “good” or “very good” outcomes. The most common psychiatric comorbidities were intellectual disabilities and attention-deficit/hyperactivity disorder. Maternal burnout and depression scores were significantly elevated compared to those of fathers. There is an undeniable urgent need for more research to identify the needs of adults and families suffering from ASD. Modifications for those with ASD may have to be made for support in workplaces, achieving driving licenses, using public transportation and attendance at tertiary education

The prevalence of childhood psychopathology in Turkey: a cross-sectional multicenter nationwide study (EPICPAT-T).

Aim: The aim of this study was to determine the prevalence of childhood psychopathologies in Turkey

The prevalence of childhood psychopathology in Turkey: a cross-sectional multicenter nationwide study (EPICPAT-T)

Conclusion: This is the largest and most comprehensive epidemiological study to determine the prevalence of psychopathologies in children and adolescents in Turkey. Our results partly higher than, and partly comparable to previous national and international studies. It also contributes to the literature by determining the independent predictors of psychopathologies in this age group