Overview of charmonium decays and production from Non-Relativistic QCD

I briefly review Non-Relativistic QCD and related effective theories, and discuss applications to heavy quarkonium decay, and production in electron-positron colliders.Comment: 8 pages, Invited talk at Charm 2010, Oct. 21-24, IHEP, Beijin

Crystal Structure of Imaginal Disc Growth Factor-2

Imaginal disc growth factor-2 (IDGF-2) is a member of a recently described family of Drosophila melanogaster-soluble polypeptide growth factors that promote cell proliferation in imaginal discs. Although their precise mode of action has not been established, IDGFs cooperate with insulin in stimulating the growth of imaginal disc cells. We report the crystal structure of IDGF-2 at 1.3-A resolution. The structure shows the classical (betaalpha)(8) barrel-fold of family 18 glycosyl hydrolases, with an insertion of an alpha + beta domain similar to that of Serratia marcescens chitinases A and B. However, amino acid substitutions in the consensus catalytic sequence of chitinases give IDGF-2 a less negatively charged environment in its putative ligand-binding site and preclude the nucleophilic attack mechanism of chitin hydrolysis. Particularly important is the replacement of Glu by Gln at position 132, which has been shown to abolish enzymatic activity in chitinases. Nevertheless, a modest conservation of residues that participate in oligosaccharide recognition suggests that IDGF-2 could bind carbohydrates, assuming several conformational changes to open the partially occluded binding site. Thus, IDGFs may have evolved from chitinases to acquire new functions as growth factors, interacting with cell surface glycoproteins implicated in growth-promoting processes, such as the Drosophila insulin receptor.Fil: Varela, Paloma F.. University of Maryland; Estados UnidosFil: Llera, Andrea Sabina. University of Maryland; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Mariuzza, Roy A.. University of Maryland; Estados UnidosFil: Tormo, José. Universidad Autónoma de Madrid; Españ

TEMPORAL AND SPATIAL DISTRIBUTION OF POACEAE POLLEN IN AREAS OF SOUTHERN UNITED KINGDOM, SPAIN AND PORTUGAL

Overall, longer Poaceae pollen seasons coincided with earlier pollen season start dates. Winter rainfall noticeably affects the intensity of Poaceae pollen seasons in Mediterranean areas, but this was not as important in Worcester. Weekly data from Worcester followed a similar pattern to that of Badajoz and Évora but at a distance of more than 1500 km and 4-5 weeks later

SarA Is an Essential Positive Regulator of Staphylococcus epidermidis Biofilm Development

Staphylococcus epidermidis biofilm formation is associated with the production of the polysaccharide intercellular adhesin (PIA)--poly-N-acetylglucosamine polysaccharide (PNAG) by the products of the icaADBC operon. Recent evidence indicates that SarA, a central regulatory element that controls the production of Staphylococcus aureus virulence factors, is essential for the synthesis of PIA/PNAG and the ensuing biofilm development in this species. Based on the presence of a sarA homolog, we hypothesized that SarA could also be involved in the regulation of the biofilm formation process in S. epidermidis. To investigate this, we constructed nonpolar sarA deletions in two genetically unrelated S. epidermidis clinical strains, O-47 and CH845. The SarA mutants were completely defective in biofilm formation, both in the steady-state conditions of a microtiter dish assay and in the flow conditions of microfermentors. Reverse transcription-PCR experiments showed that the mutation in the sarA gene resulted in downregulation of the icaADBC operon transcription in an IcaR-independent manner. Purified SarA protein showed high-affinity binding to the icaA promoter region by electrophoretic mobility shift assays. Consequently, mutation in sarA provoked a significant decrease in the amount of PIA/PNAG on the cell surface. Furthermore, heterologous complementation of S. aureus sarA mutants with the sarA gene of S. epidermidis completely restored biofilm formation. In summary, SarA appeared to be a positive regulator of transcription of the ica locus, and in its absence, PIA/PNAG production and biofilm formation were diminished. Additionally, we present experimental evidence showing that SarA may be an important regulatory element that controls S. epidermidis virulence factors other than biofilm formation

The use of branch piles to assist in the restoration of degraded semiarid steppes

Desertification is a major environmental problem in arid and semiarid regions. Tree plantation has been commonly employed to foster the recovery of degraded areas. However, this technique is costly, and their outcomes are often uncertain. Therefore, we evaluated an alternative method for the restoration of degraded semiarid steppes that involved the construction of branch piles to attract frugivores as potential seed-dispersing birds, promoting seed rain, and fostering the formation of woody patches. We measured the success of branch piles in terms of the number of bird visits and seed input compared to naturally occurring shrub patches. Generally, frugivorous birds visited branch piles less frequently than shrub patches. Yet, branch piles accumulated seeds of patch-forming shrub species. Seed rain was higher under patches of the dominant shrub Rhamnus lycioides than under branch piles. In contrast, woody patches and branch piles did not differ in seed input of the less abundant Pistacia lentiscus shrub. Our study demonstrates that branch piles are used by frugivorous birds and accumulate seeds of patch-forming shrubs. Branch piles may be a suitable method to promote the expansion of bird-dispersed plant species and restore semiarid wooded steppes. However, their efficiency largely depends on pile persistence and economic cost

A multihost bacterial pathogen overcomes continuous population bottlenecks to adapt to new host species

While many bacterial pathogens are restricted to single host species, some have the capacity to undergo host switches, leading to the emergence of new clones that are a threat to human and animal health. However, the bacterial traits that underpin a multihost ecology are not well understood. Following transmission to a new host, bacterial populations are influenced by powerful forces such as genetic drift that reduce the fixation rate of beneficial mutations, limiting the capacity for host adaptation. Here, we implement a novel experimental model of bacterial host switching to investigate the ability of the multihost pathogen Staphylococcus aureus to adapt to new species under continuous population bottlenecks. We demonstrate that beneficial mutations accumulated during infection can overcome genetic drift and sweep through the population, leading to host adaptation. Our findings highlight the remarkable capacity of some bacteria to adapt to distinct host niches in the face of powerful antagonistic population forces.status: publishe

Semejanzas y diferencias entre dos estaciones aerobiológicas

XV lnternational A.P.L.E. Symposium of Palynolog

Could Technology and Intelligent Transport Systems Help Improve Mobility in an Emerging Country? Challenges, Opportunities, Gaps and Other Evidence from the Caribbean

Apart from constituting a topic of high relevance for transport planners and policymakers, support technologies for traffic have the potential to bring significant benefits to mobility. In addi- tion, there are groups of 'high potential' users, such as young adults, who constitute an essential part of the current market. Notwithstanding, and especially in low and middle-income countries (LMICs), their knowledge and acceptance remain understudied. This study aimed to assess the appraisal of intelligent transport systems (ITS) and other technological developments applicable to mobility among Dominican young adults. Methods: In this study, we used the data gathered from 1414 Dominicans aged between 18 and 40, responding to the National Survey on Mobility in 2018 and 2019. Results: Overall, and although there is a relatively high acceptance, attributed value, and attitudinal predisposition towards both intelligent transportation systems and various support technologies applicable to mobility, the actual usage rates remain considerably low, and this is probably exacerbated by the low and middle-income status of the country. Conclusions: The findings of this study suggest the need to strengthen information and communication flows over emerging mobility-related technologies and develop further awareness of the potential benefits of technological developments for everyday transport dynamics

Muon decay in orbit: spectrum of high-energy electrons

Experimental searches for lepton-flavor-violating coherent muon-to-electron conversion in the field of a nucleus, have been proposed to reach the unprecedented sensitivity of 10^(-16) - 10^(-18) per stopped muon. At that level, they probe new interactions at effective-mass scales well beyond 1000 TeV. However, they must contend with background from ordinary bound muon decay. To better understand the background-spectrum shape and rate, we have carried out a detailed analysis of Coulombic-bound-state muon decay, including nuclear recoil. Implications for future experiments are briefly discussed.Comment: 11 pages, 6 figures. Accepted for publication in Phys.Rev.D. v2: One sentence modified to avoid possible misunderstandings. Journal versio