Capacitación y supervisión a equipos técnicos: saldando una deuda pendiente en temas de discapacidad y vejez

El presente trabajo hace referencia a la experiencia que surge de la implementación del proyecto aprobado y financiado en 2010 por Extensión Universitaria de la UNLP, denominado “Capacitación y Supervisión a equipos interdisciplinarios: saldando una deuda pendiente en temas de vejez y discapacidad”. Fueron partícipes de esta experiencia estudiantes avanzados, graduados y profesores de las Facultades de Humanidades y Ciencias de la Educación, Psicología y Trabajo Social (en carácter de Unidad Ejecutora), y como co-participantes la Comisión Universitaria sobre Discapacidad (CUD), el INSSJyP – Pami, la Red Mayor Nodo La Plata y la Dirección de Servicios Sociales de la UNLP. La implementación del proyecto implicó las instancias de capacitación y supervisión institucional, destinado a equipos técnicos y profesionales que se desempeñaban en centros de salud, áreas de acción social municipal, organizaciones específicas de adultos mayores e instituciones de personas con discapacidad. La implementación del proyecto dio lugar a que se establecieran tres aspectos a analizar al momento de realizar la evaluación final del mismo: a) la realización de trabajos finales de los diversos destinatarios que con sus diferentes matrices disciplinarias problematizaron la temática de la vejez y la discapacidad, la apropiación de nuevos conocimientos que potenciaron el cuestionamiento de las prácticas vigentes, la incorporación de metodologías de análisis diagnóstico, el conocimiento, diseño e implementación de modelos de gestión y el fortalecimiento del trabajo interdisciplinario; b) la función de Extensión Universitaria de responder a las demandas de la sociedad ante las carencias en la formación académica de los graduados; y finalmente c) impulsar la formación de estudiantes avanzados que a través de la extensión acompañaron el proyecto y participaron activamente en el cumplimiento del mismo. Por otro lado, se debe destacar que esta experiencia de intervención aporta a la política pública a partir del trabajo multidimensional de los diversos actores implicados en la propuesta (Universidad; Estado Nacional; Organizaciones de la Sociedad Civil; Ciudadanos), así como la intención del proyecto en sí mismo de buscar constantemente el conocimiento y la reflexión que permita mejorar la calidad de trabajo de los profesionales, generar ambientes laborales saludables en las diversas instituciones, y consecuentemente contribuir a la mejora de la calidad de vida de quienes reciben los aportes profesionales, en este caso, la población adulta mayor y las personas con discapacidad

International Network for Capacity Building for the Control of Emerging Viral Vector-Borne Zoonotic Diseases: Arbo-Zoonet

Arboviruses are arthropod-borne viruses, which include West Nile fever virus (WNFV), a mosquito-borne virus, Rift Valley fever virus (RVFV), a mosquito-borne virus, and Crimean-Congo haemorrhagic fever virus (CCHFV), a tick-borne virus. These arthropod-borne viruses can cause disease in different domestic and wild animals and in humans, posing a threat to public health because of their epidemic and zoonotic potential. In recent decades, the geographical distribution of these diseases has expanded. Outbreaks of WNF have already occurred in Europe, especially in the Mediterranean basin. Moreover, CCHF is endemic in many European countries and serious outbreaks have occurred, particularly in the Balkans, Turkey and Southern Federal Districts of Russia. In 2000, RVF was reported for the first time outside the African continent, with cases being confirmed in Saudi Arabia and Yemen. This spread was probably caused by ruminant trade and highlights that there is a threat of expansion of the virus into other parts of Asia and Europe. In the light of global warming and globalisation of trade and travel, public interest in emerging zoonotic diseases has increased. This is especially evident regarding the geographical spread of vector-borne diseases. A multi-disciplinary approach is now imperative, and groups need to collaborate in an integrated manner that includes vector control, vaccination programmes, improved therapy strategies, diagnostic tools and surveillance, public awareness, capacity building and improvement of infrastructure in endemic regions

On-sky results of the adaptive optics MACAO for the new IR-spectrograph CRIRES at VLT

The adaptive optics MACAO has been implemented in 6 focii of the VLT observatory, in three different flavors. We present in this paper the results obtained during the commissioning of the last of these units, MACAO-CRIRES. CRIRES is a high-resolution spectrograph, which efficiency will be improved by a factor two at least for point-sources observations with a NGS brighter than R=15. During the commissioning, Strehl exceeding 60% have been observed with fair seeing conditions, and a general description of the performance of this curvature adaptive optics system is done.Comment: SPIE conference 2006, Advances in adaptive optics, 12 pages, 11 figure

Molecular diagnostic and genetic characterization of highly pathogenic viruses: application during Crimean–Congo haemorrhagic fever virus outbreaks in Eastern Europe and the Middle East

AbstractSeveral haemorrhagic fevers are caused by highly pathogenic viruses that must be handled in Biosafety level 4 (BSL–4) containment. These zoonotic infections have an important impact on public health and the development of a rapid and differential diagnosis in case of outbreak in risk areas represents a critical priority. We have demonstrated the potential of a DNA resequencing microarray (PathogenID v2.0) for this purpose. The microarray was first validated in vitro using supernatants of cells infected with prototype strains from five different families of BSL-4 viruses (e.g. families Arenaviridae, Bunyaviridae, Filoviridae, Flaviviridae and Paramyxoviridae). RNA was amplified based on isothermal amplification by Phi29 polymerase before hybridization. We were able to detect and characterize Nipah virus and Crimean–Congo haemorrhagic fever virus (CCHFV) in the brains of experimentally infected animals. CCHFV was finally used as a paradigm for epidemics because of recent outbreaks in Turkey, Kosovo and Iran. Viral variants present in human sera were characterized by BLASTN analysis. Sensitivity was estimated to be 105–106 PFU/mL of hybridized cDNA. Detection specificity was limited to viral sequences having ˜13–14% of global divergence with the tiled sequence, or stretches of ˜20 identical nucleotides. These results highlight the benefits of using the PathogenID v2.0 resequencing microarray to characterize geographical variants in the follow-up of haemorrhagic fever epidemics; to manage patients and protect communities; and in cases of bioterrorism

High binding yet accelerated guest rotation within a cucurbit[7]uril complex. Toward paramagnetic gyroscopes and rolling nanomachines †

International audienceThe (15-oxo-3,7,11-triazadispiro[5.1.5.3]hexadec-7-yl)oxidanyl, a bis-spiropiperidinium nitroxide derived from TEMPONE, can be included in cucurbit[7]uril to form a strong (K a ∼ 2 × 10 5 M −1) CB[7]@bPTO complex. EPR and MS spectra, DFT calculations, and unparalleled increased resistance (a factor of ∼10 3) toward ascorbic acid reduction show evidence of deep inclusion of bPTO inside CB[7]. The unusual shape of the CB[7]@bPTO EPR spectrum can be explained by an anisotropic Brownian rotational diffusion, the global tumbling of the complex being slower than rotation of bPTO around its " long molecular axis " inside CB[7]. The CB[7] (stator) with the encapsulated bPTO (rotator) behaves as a supramolecular para-magnetic rotor with increased rotational speed of the rotator that has great potential for advanced nano-scale machines requiring wheels such as cucurbiturils with virtually no friction between the wheel and the axle for optimum wheel rotation (i.e. nanopulleys and nanocars)

Resolving Stellar Populations outside the Local Group: MAD observations of UKS2323-326

We present a study aimed at deriving constraints on star formation at intermediate ages from the evolved stellar populations in the dwarf irregular galaxy UKS2323-326. These observations were also intended to demonstrate the scientific capabilities of the multi-conjugated adaptive optics demonstrator (MAD) implemented at the ESO Very Large Telescope as a test-bench of adaptive optics (AO) techniques. We perform accurate, deep photometry of the field using J and Ks band AO images of the central region of the galaxy. The near-infrared (IR) colour-magnitude diagrams clearly show the sequences of asymptotic giant branch (AGB) stars, red supergiants, and red giant branch (RGB) stars down to ~1 mag below the RGB tip. Optical-near-IR diagrams, obtained by combining our data with Hubble Space Telescope observations, provide the best separation of stars in the various evolutionary stages. The counts of AGB stars brighter than the RGB tip allow us to estimate the star formation at intermediate ages. Assuming a Salpeter initial mass function, we find that the star formation episode at intermediate ages produced ~6x10^5 M_sun of stars in the observed region.Comment: 4 pages, 4 figures, Accepted for publication in A&A Letter

MCAO near-IR photometry of the Globular Cluster NGC 6388: MAD observations in crowded fields

Deep photometry of crowded fields, such as Galactic Globular Clusters, is severely limited by the actual resolution of ground-based telescopes. On the other hand, the Hubble Space Telescope does not provide the near-infrared (NIR) filters needed to allow large color baselines. In this work we aim at demonstrating how ground based observations can reach the required resolution when using Multi-Conjugated Adaptive Optic (MCAO) devices in the NIR, such as the experimental infrared camera (MAD) available on the VLT. This is particularly important since these corrections are planned to be available on all ground--based telescopes in the near future. We do this by combining the infrared photometry obtained by MAD/VLT with ACS/HST optical photometry of our scientific target, the bulge globular cluster NGC 6388, in which we imaged two fields. In particular, we constructed color-magnitude diagrams with an extremely wide color baseline in order to investigate the presence of multiple stellar populations in this cluster. From the analysis of the external field, observed with better seeing conditions, we derived the deepest optical-NIR CMD of NGC 6388 to date. The high-precision photometry reveals that two distinct sub-giant branches are clearly present in this cluster. We also use the CMD from the central region to estimate the distance ((m-M)=15.33) and the reddening (E(B-V)=0.38) for this cluster. We estimate the age to be ~11.5+/- 1.5 Gyr. The large relative-age error reflects the bimodal distribution of the SGB stars. This study clearly demonstrates how MCAO correction in the NIR bands implemented on ground based telescopes can complement the high-resolution optical data from HST.Comment: 9 pages, 10 figures. Accepted for publication on A. &

Genomic Diversity and Evolution of the Lyssaviruses

Lyssaviruses are RNA viruses with single-strand, negative-sense genomes responsible for rabies-like diseases in mammals. To date, genomic and evolutionary studies have most often utilized partial genome sequences, particularly of the nucleoprotein and glycoprotein genes, with little consideration of genome-scale evolution. Herein, we report the first genomic and evolutionary analysis using complete genome sequences of all recognised lyssavirus genotypes, including 14 new complete genomes of field isolates from 6 genotypes and one genotype that is completely sequenced for the first time. In doing so we significantly increase the extent of genome sequence data available for these important viruses. Our analysis of these genome sequence data reveals that all lyssaviruses have the same genomic organization. A phylogenetic analysis reveals strong geographical structuring, with the greatest genetic diversity in Africa, and an independent origin for the two known genotypes that infect European bats. We also suggest that multiple genotypes may exist within the diversity of viruses currently classified as ‘Lagos Bat’. In sum, we show that rigorous phylogenetic techniques based on full length genome sequence provide the best discriminatory power for genotype classification within the lyssaviruses

Emerging Technologies for the Detection of Rabies Virus: Challenges and Hopes in the 21st Century

The diagnosis of rabies is routinely based on clinical and epidemiological information, especially when exposures are reported in rabies-endemic countries. Diagnostic tests using conventional assays that appear to be negative, even when undertaken late in the disease and despite the clinical diagnosis, have a tendency, at times, to be unreliable. These tests are rarely optimal and entirely dependent on the nature and quality of the sample supplied. In the course of the past three decades, the application of molecular biology has aided in the development of tests that result in a more rapid detection of rabies virus. These tests enable viral strain identification from clinical specimens. Currently, there are a number of molecular tests that can be used to complement conventional tests in rabies diagnosis. Indeed the challenges in the 21st century for the development of rabies diagnostics are not of a technical nature; these tests are available now. The challenges in the 21st century for diagnostic test developers are two-fold: firstly, to achieve internationally accepted validation of a test that will then lead to its acceptance by organisations globally. Secondly, the areas of the world where such tests are needed are mainly in developing regions where financial and logistical barriers prevent their implementation. Although developing countries with a poor healthcare infrastructure recognise that molecular-based diagnostic assays will be unaffordable for routine use, the cost/benefit ratio should still be measured. Adoption of rapid and affordable rabies diagnostic tests for use in developing countries highlights the importance of sharing and transferring technology through laboratory twinning between the developed and the developing countries. Importantly for developing countries, the benefit of molecular methods as tools is the capability for a differential diagnosis of human diseases that present with similar clinical symptoms. Antemortem testing for human rabies is now possible using molecular techniques. These barriers are not insurmountable and it is our expectation that if such tests are accepted and implemented where they are most needed, they will provide substantial improvements for rabies diagnosis and surveillance. The advent of molecular biology and new technological initiatives that combine advances in biology with other disciplines will support the development of techniques capable of high throughput testing with a low turnaround time for rabies diagnosis

Solid-State NMR/Dynamic Nuclear Polarization of Polypeptides in Planar Supported Lipid Bilayers

Dynamic nuclear polarization has been developed to overcome the limitations of the inherently low signal intensity of NMR spectroscopy. This technique promises to be particularly useful for solid-state NMR spectroscopy where the signals are broadened over a larger frequency range and most investigations rely on recording low gamma nuclei. To extend the range of possible investigations, a triple-resonance flat-coil solid-state NMR probe is presented with microwave irradiation capacities allowing the investigation of static samples at temperatures of 100 K, including supported lipid bilayers. The probe performance allows for two-dimensional separated local field experiments with high-power Lee-Goldberg decoupling and cross-polarization under simultaneous irradiation from a gyrotron microwave generator. Efficient cooling of the sample turned out to be essential for best enhancements and line shape and necessitated the development of a dedicated cooling chamber. Furthermore, a new membrane-anchored biradical is presented, and the geometry of supported membranes was optimized not only for good membrane alignment, handling, stability, and filling factor of the coil but also for heat and microwave dissipation. Enhancement factors of 17-fold were obtained, and a two-dimensional PISEMA spectrum of a transmembrane helical peptide was obtained in less than 2 h