Concept of solar energy utilization DpmP

Práce se zabývá především vhodným výběrem střešních ploch Dopravního podniku města Pardubic a. s., pro umístění fotovoltaických panelů. Dále pak řeší i dispoziční otázku fotovoltaických panelů a jejich výkon.The paper mainly focuses on a suitable selection of roof areas of The Pardubice public transit Co. Inc., for the placement of photovolatic panels. Furthermore, it tries to settle the issue of the photovolatic panels and their performance.Katedra elektrotechniky, elektroniky a zabezpečovací techniky v dopravěDokončená práce s úspěšnou obhajobo

Simulation of electricity network - Smart Grid

Práce je zaměřena především na simulaci přenosu dat po energetickém vedení. Teoretická část práce se zabývá popisem parametrů elektrického vedení, vlastností statických elektroměrů a nejčastěji používaných modulací pro přenos dat. V této části jsou rovněž popsány možnosti připojení vysílačů a přijímačů na energetickou síť, frekvenční rozsahy, ve kterých je komunikace povolena, dle normy ČSN EN 50065. V praktické části jsou provedeny simulace přenosu digitálního signálu po energetickém vedení různých délek a zatížení.The work is mainly focused on the simulation data for energy lines. The theoretical part deals with the description of the parameters of power lines, properties of static electrical and frequently used modulation for transfer data. This section also describes the connectivity of transmitters and receivers on power network, frequency ranges in which communication is allowed, according to Standard EN 50065. The practical part of the simulated transmission of digital signals over power lines of different lengths and loads.Katedra elektrotechniky, elektroniky a zabezpečovací techniky v dopravěUchazeč prezentoval komisi hlavní výsledky a závěry své diplomové práce. Po prezentaci diplomové práce zodpověděl dotazy vedoucího a oponenta diplomové práce. V diskusi o diplomové práci podrobně zodpověděl všechny dotazy členů komise.Dokončená práce s úspěšnou obhajobo

Optimization of the HPLC method for determination of propranolol in liquid oral formulations

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tomáš Koval Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Optimization of the HPLC method for determination of propranolol in liquid oral formulations The purpose of thesis is the optimization and subsequent validation of HPLC method for determination of propranolol - hydrochloride in liquid oral formulations. A medicinal product containing propranolol - hydrochloride is used in the therapy of infantile haemangiomas as extemporaneous prepared solution. For method optimization were chosen Kinetex® C18 column (150x4,6 mm, size of particles 5 μm) and isocratic elution of mobile phase containing phosphate buffer (c=17,7 mM) and acetonitrile in 30:70 ratio, with pH adjusted to 2,2. Flow rate was set at 1,2 ml/min. According to results of the validation, was found that this developed method for the analysis of the product is suitable, and provides accurate and precise results. Keywords: propranolol hydrochloride, HPLC, optimization, Sodium benzoate, liquid oral formulation

Velkoobjemové stohování vzorků antiepileptik při protiproudé elektroforéze prováděné v kapiláře s povlakem PAMAPTAC

Electrophoretic stacking is developed for sensitive determination of three zwitterionic antiepileptics, namely vigabatrin, pregabalin and gabapentin, in human serum. CE separation is performed in a 25 mu m fused silica capillary covalently coated with the copolymer of acrylamide with 5% content of permanently charged 3-acryl-amidopropyl trimethylammonium chloride (PAMAPTAC). In background electrolyte of 500 mM acetic acid, the 5% PAMAPTAC generates an anodic electm-osmotic flow with a magnitude of (-18.6 +/- 0.5) x 10(-9) m(2)V(-1)s(-1), which acts against the direction of the electrophoretic migration of the analytes. A sample of the antiepileptic prepared in a 25% v/v infusion solution and 75% v/v acetonitrile is injected into the capillary in a large volume attaining a zone length of up to 270 mm. After turning on the separation voltage, the antiepileptics are isotachophoretically focussed behind the zone of Na+ ions with a sensitivity enhancement factor of 78. For the clinical determination of antiepileptics, the human serum is diluted with acetonitrile in a ratio of 1:3 v/v and a zone with a length of 90 mm is injected into the capillary. The method is linear in the 0.025-2.5 mu g/mL concentration range; the attained limit of quantification is in the range 18.3-22.8 nmol/L; the within-day precision for the migration time is 0.8-1.2% and for the peak area 1.5-2.4%.Elektroforetické stohování je vyvinuto pro citlivé stanovení tří zwitteriontových antiepileptik, konkrétně vigabatrinu, pregabalinu a gabapentinu, v lidském séru. CE separace se provádí v 25 mu m kapiláře z taveného oxidu křemičitého kovalentně potažené kopolymerem akrylamidu s 5% obsahem trvale nabitého 3-akryl-amidopropyltrimethylamonium chloridu (PAMAPTAC). V elektrolytu pozadí 500 mM kyseliny octové vytváří 5% PAMAPTAC anodický elektroosmotický tok o velikosti (-18,6 +/- 0,5) x 10(-9) m(2)V(-1)s(-1), který působí proti směru elektroforetické migrace analytů. Vzorek antiepileptika připravený v 25% v/v infuzním roztoku a 75% v/v acetonitrilu se vstříkne do kapiláry ve velkém objemu a dosáhne délky zóny až 270 mm. Po zapnutí separačního napětí se antiepileptika izotachoforeticky soustředí za zónu iontů Na+ s faktorem zvýšení citlivosti 78. Pro klinické stanovení antiepileptik se lidské sérum zředí acetonitrilem v poměru 1:3 v/v a do kapiláry se nastříkne zóna o délce 90 mm. Metoda je lineární v koncentračním rozmezí 0,025-2,5 mu g/ml; dosažená mez stanovitelnosti je v rozmezí 18,3-22,8 nmol/l; přesnost v rámci dne pro migrační čas je 0,8-1,2 % a pro plochu píku 1,5-2,4 %

Atomic resolution studies of S1 nuclease complexes reveal details of RNA interaction with the enzyme despite multiple lattice-translocation defects

S1 nuclease from Aspergillus oryzae is a single-strand-specific nuclease from the S1/P1 family that is utilized in biochemistry and biotechnology. S1 nuclease is active on both RNA and DNA but with differing catalytic efficiencies. This study clarifies its catalytic properties using a thorough comparison of differences in the binding of RNA and DNA in the active site of S1 nuclease based on X-ray structures, including two newly solved complexes of S1 nuclease with the products of RNA cleavage at atomic resolution. Conclusions derived from this comparison are valid for the whole S1/P1 nuclease family. For proper model building and refinement, multiple lattice-translocation defects present in the measured diffraction data needed to be solved. Two different approaches were tested and compared. Correction of the measured intensities proved to be superior to the use of the dislocation model of asymmetric units with partial occupancy of individual chains. As the crystals suffered from multiple lattice translocations, equations for their correction were derived de novo. The presented approach to the correction of multiple lattice-translocation defects may help to solve similar problems in the field of protein X-ray crystallography

Crystallographic fragment screening-based study of a novel FAD-dependent oxidoreductase from Chaetomium thermophilum

The FAD-dependent oxidoreductase from Chaetomium thermophilum (CtFDO) is a novel thermostable glycoprotein from the glucose–methanol–choline (GMC) oxidoreductase superfamily. However, CtFDO shows no activity toward the typical substrates of the family and high-throughput screening with around 1000 compounds did not yield any strongly reacting substrate. Therefore, protein crystallography, including crystallographic fragment screening, with 42 fragments and 37 other compounds was used to describe the ligand-binding sites of CtFDO and to characterize the nature of its substrate. The structure of CtFDO reveals an unusually wide-open solvent-accessible active-site pocket with a unique His–Ser amino-acid pair putatively involved in enzyme catalysis. A series of six crystal structures of CtFDO complexes revealed five different subsites for the binding of aryl moieties inside the active-site pocket and conformational flexibility of the interacting amino acids when adapting to a particular ligand. The protein is capable of binding complex polyaromatic substrates of molecular weight greater than 500 Da