Constructive alignment in the built environment: enhancing teaching in line with graduate outcomes

The paper examines the manner in which to review an undergraduate degree in construction management using a top-down approach known as “Constructive Alignment”. The research addresses not only the perceived teaching problems, but it also discusses the methods used to rejuvenate the course in a manner that aligns with the graduate outcomes. However, it was also clear that teaching staff were not especially aware of the need to address the course learning outcomes. This highlighted the need for teaching staff to be involved in a process of constructive alignment to embed the course learning outcomes within their subjects, while also addressing the teaching issues involved with assessment. This process provided an opportunity to determine the incremental skill and knowledge development, both within the subjects, as well as between subjects across the course. The paper concludes with the production of a conceptual framework, which can be used to assist with the alignment of professional standards, course outcomes and graduate attributes into a discipline-specific degree program

Characterizing the pathotype of neonatal meningitis causing <i>Escherichia coli</i> (NMEC)

Background Neonatal meningitis-causing Escherichia coli (NMEC) is the predominant Gram-negative bacterial pathogen associated with meningitis in newborn infants. High levels of heterogeneity and diversity have been observed in the repertoire of virulence traits and other characteristics among strains of NMEC making it difficult to define the NMEC pathotype. The objective of the present study was to identify genotypic and phenotypic characteristics of NMEC that can be used to distinguish them from commensal E. coli. Methods A total of 53 isolates of NMEC obtained from neonates with meningitis and 48 isolates of fecal E. coli obtained from healthy individuals (HFEC) were comparatively evaluated using five phenotypic (serotyping, serum bactericidal assay, biofilm assay, antimicorbial susceptibility testing, and in vitro cell invasion assay) and three genotypic (phylogrouping, virulence genotyping, and pulsed-field gel electrophoresis) methods. Results A majority (67.92 %) of NMEC belonged to B2 phylogenetic group whereas 59 % of HFEC belonged to groups A and D. Serotyping revealed that the most common O and H types present in NMEC tested were O1 (15 %), O8 (11.3 %), O18 (13.2 %), and H7 (25.3 %). In contrast, none of the HFEC tested belonged to O1 or O18 serogroups. The most common serogroup identified in HFEC was O8 (6.25 %). The virulence genotyping reflected that more than 70 % of NMEC carried kpsII, K1, neuC, iucC, sitA, and vat genes with only less than 27 % of HFEC possessing these genes. All NMEC and 79 % of HFEC tested were able to invade human cerebral microvascular endothelial cells. No statistically significant difference was observed in the serum resistance phenotype between NMEC and HFEC. The NMEC strains demonstrated a greater ability to form biofilms in Luria Bertani broth medium than did HFEC (79.2 % vs 39.9 %). Conclusion The results of our study demonstrated that virulence genotyping and phylogrouping may assist in defining the potential NMEC pathotype

The bHLH transcription factor SPATULA enables cytokinin signaling, and both activate auxin biosynthesis and transport genes at the medial domain of the gynoecium

[EN] Fruits and seeds are the major food source on earth. Both derive from the gynoecium and, therefore, it is crucial to understand the mechanisms that guide the development of this organ of angiosperm species. In Arabidopsis, the gynoecium is composed of two congenitally fused carpels, where two domains: medial and lateral, can be distinguished. The medial domain includes the carpel margin meristem (CMM) that is key for the production of the internal tissues involved in fertilization, such as septum, ovules, and transmitting tract. Interestingly, the medial domain shows a high cytokinin signaling output, in contrast to the lateral domain, where it is hardly detected. While it is known that cytokinin provides meristematic properties, understanding on the mechanisms that underlie the cytokinin signaling pattern in the young gynoecium is lacking. Moreover, in other tissues, the cytokinin pathway is often connected to the auxin pathway, but we also lack knowledge about these connections in the young gynoecium. Our results reveal that cytokinin signaling, that can provide meristematic properties required for CMM activity and growth, is enabled by the transcription factor SPATULA (SPT) in the medial domain. Meanwhile, cytokinin signaling is confined to the medial domain by the cytokinin response repressor ARABIDOPSIS HISTIDINE PHOSPHOTRANSFERASE 6 (AHP6), and perhaps by ARR16 (a type-A ARR) as well, both present in the lateral domains (presumptive valves) of the developing gynoecia. Moreover, SPT and cytokinin, probably together, promote the expression of the auxin biosynthetic gene TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (TAA1) and the gene encoding the auxin efflux transporter PIN-FORMED 3 (PIN3), likely creating auxin drainage important for gynoecium growth. This study provides novel insights in the spatiotemporal determination of the cytokinin signaling pattern and its connection to the auxin pathway in the young gynoecium.IRO, VMZM, HHU and PLS were supported by the Mexican National Council of Science and Technology (CONACyT) with a PhD fellowship (210085, 210100, 243380 and 219883, respectively). Work in the SDF laboratory was financed by the CONACyT grants CB-2012-177739, FC-2015-2/1061, and INFR-2015-253504, and NMM by the CONACyT grant CB-2011-165986. SDF, CF and LC acknowledge the support of the European Union FP7-PEOPLE-2009-IRSES project EVOCODE (grant no. 247587) and H2020-MSCARISE-2015 project ExpoSEED (grant no. 691109). SDF also acknowledges the Marine Biological Laboratory (MBL) in Woods Hole for a scholarship for the Gene Regulatory Networks for Development Course 2015 (GERN2015). IE acknowledges the International European Fellowship-METMADS project and the Universita degli Studi di Milano (RTD-A; 2016). Research in the laboratory of MFY was funded by NSF (grant IOS-1121055), NIH (grant 1R01GM112976-01A1) and the Paul D. Saltman Endowed Chair in Science Education (MFY). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Reyes Olalde, J.; Zuñiga, V.; Serwatowska, J.; Chávez Montes, R.; Lozano-Sotomayor, P.; Herrera-Ubaldo, H.; Gonzalez Aguilera, K.... (2017). The bHLH transcription factor SPATULA enables cytokinin signaling, and both activate auxin biosynthesis and transport genes at the medial domain of the gynoecium. PLoS Genetics. 13(4):1-31. https://doi.org/10.1371/journal.pgen.1006726S131134Reyes-Olalde, J. I., Zuñiga-Mayo, V. M., Chávez Montes, R. A., Marsch-Martínez, N., & de Folter, S. (2013). Inside the gynoecium: at the carpel margin. Trends in Plant Science, 18(11), 644-655. doi:10.1016/j.tplants.2013.08.002Alvarez-Buylla, E. 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Student participation in professional organisations: the need for innovation

Professional organisations in construction management and builtenvironment list many benefits of membership, including professionaldevelopment and engagement with fellow professionals. However,despite free membership for students, their membership and retention isgenerally low and it is important for the future of professionalorganisations to determine reasons for this disinterest. Do currentstudents understand the benefits of professional organisations and placeany value on membership? This paper presents the findings of a researchproject to better understand why recruitment of students and their activeparticipation in professional organisations is low. A questionnaire surveywas conducted across the students of the degrees of constructionmanagement in Deakin University to obtain the evaluations of themembership, while interviews were undertaken with representatives ofprofessional organisations to determine the alignment between theparties. Student expectations of membership were shown to be amismatch with the stated benefits offered by the professionalorganisations, or at least with how these benefits are communicated.Possible improvements are suggested to ensure that, in this era of instantcommunications and access to information, professional organisationsinnovate to remain relevant to the future leaders of their industry

Teaching collaboration in tertiary BIM education: A review and analysis

Building Information Modelling (BIM) is inherently a team-based activity and possessing the Knowledge, Skills, and Abilities (KSAs) to collaborate across various disciplines and organisations are central for BIM-ready graduates. There is, nevertheless, no systematic attempt to analyse the current approaches to teaching collaboration KSAs across tertiary education institutions. This study is an attempt to provide an account of the current approaches taken by leading universities for training and educating collaboration, as a part of their BIM education programs. To this end, 63 articles on BIM education were identified, of which 18 were used to extract their practices to teaching collaboration. The content analysis of these studies revealed that the practices identified can be presented under 4 broad categories including Instructional approach, Prerequisites, Marking and evaluation, and Feedback, in which each category has various sub-categories. As the first in its kind, the present study provides an analysis and synthesis of the best practices for teaching educating collaboration, and therefore, assists educators and curriculum builders in integrating collaboration KSAs into BIM education in construction-related curricula

Online delivery models: Students’ perspectives

The optimal delivery model for units always puzzle curriculum designers and lecturers, particularly when the unit is offered in the summer trimester and students have greater choice as to whether to enrol in a unit or not. An ongoing research project in the School of Architecture and Built Environment at Deakin University aims to understand students’ perceptions on unit delivery in the summer trimester in order to improve support for online delivery models. The five delivery models in the study ranged from ‘traditional’ i.e. on campus lectures and tutorials for each week of the trimester; to ‘wholly online’ i.e. learning materials and communications entirely through the web-based student portal. Students rated their preferences for the five delivery models with additional comments. Students overwhelmingly prefer wholly online delivery during the summer trimester despite the benefits of other delivery models and that wholly online delivery may not offer their preferred learning experience. The students’ primary need is for flexibility which can be at odds with their equal need for interaction with academics and peers. It is important that academics recognise students’ perspectives to ensure their design of online delivery models improves teaching and learning in the summer trimester

Association of iss and iucA, but Not tsh, with Plasmid-Mediated Virulence of Avian Pathogenic Escherichia coli

Avian pathogenic Escherichia coli (APEC) is an economically important respiratory pathogen of chickens worldwide. Factors previously associated with the virulence of APEC include adhesins, iron-scavenging mechanisms, the production of colicin V (ColV), serum resistance, and temperature-sensitive hemagglutination, but virulence has generally been assessed by parenteral inoculation, which does not replicate the normal respiratory route of infection. A large plasmid, pVM01, is essential for virulence in APEC strain E3 in chickens after aerosol exposure. Here we establish the size of pVM01 to be approximately 160 kb and show that the putative virulence genes iss (increased serum survival) and tsh (temperature-sensitive hemagglutinin) and the aerobactin operon are on the plasmid. These genes were not clustered on pVM01 but, rather, were each located in quite distinct regions. Examination of APEC strains with defined levels of respiratory pathogenicity after aerosol exposure showed that both the aerobactin operon and iss were associated with high levels of virulence in APEC but that the possession of either gene was sufficient for intermediate levels of virulence. In constrast, the presence of tsh was not necessary for high levels of virulence. Thus, both the aerobactin operon and iss are associated with virulence in APEC after exposure by the natural route of infection. The similarities between APEC and extraintestinal E. coli infection in other species suggests that they may be useful models for definition of the role of these virulence genes and of other novel virulence genes that may be located on their virulence plasmids