Early Cell-Cell Coupling Impairs Stem Cell Retention when Co-cultured with Hypoxic Cardiomyocytes

Rationale: Bone marrow-derived mesenchymal stem cells (BM-MSC) induce beneficial effects in the heart following hypoxia but loss of these cells would diminish their effect. We hypothesized that coupling between BM-MSC and cardiomyocytes (CM) through gap junctions (GJ) during hypoxia may play an important role in stem cell retention. Methods and Results: Both human and murine BM-MSC express connexin43 (Cx43), a critical protein in the formation of GJ. FACS analysis revealed that BM-MSC exhibited a significant reduction in apoptosis (3-5 fold;

WANDA, a regional dynamic nitrogen model (With Aggregated Nitrogen DynAmics) for nitrate leaching from forests

International audienceNitrate concentrations in recently infiltrated groundwater in forested areas in the Netherlands are slowly increasing towards the EU limit of 50 mg NO3 l?1. The origin of this nitrate is thought to be leaching from nitrogen (N) saturated semi-natural ecosystems in these areas. To simulate nitrate leaching on a regional scale, the empirical model WANDA (a regional nitrogen model With Aggregated Nitrogen DynAmics) is introduced. The model is built around the concept that in forests the C:N ratio of the organic layer is indicative of the amount of nitrate leaving the system in drainage. WANDA was tested on a regional dataset of an infiltration area of 10 km2. This infiltration area consisted of 350 forest stands draining to the catchment "Edese Bos" used by a drinking water company. In 75 of the 350 forest stands the C:N ratio of the organic layer was measured. In 30 of these 75 stands, the nitrate concentrations below the rooting zone were measured. A hydrological model calculated water fluxes. The C:N ratio of the 75 stands ranged from 15.7 to 31.3 g C g?1 N. Scots pine stands had the highest C:N ratios in the organic layer. Nitrate concentrations varied in the 30 stands from 0.6 to 70 mg NO3 l?1. The nitrate concentrations in the Douglas fir stands were higher, and in the beech stands lower, than those observed in the locations with other tree species. WANDA predicted nitrate concentrations well, but improvements are required to simulate nitrate fluxes. The simulation of the water fluxes in WANDA is probably where more focussed modelling effort is now required. Keywords: nitrogen, nitrate, forest, model, WANDA, C:N ratio, organic laye

Applying MERLIN for modelling nitrate leaching in a nitrogen saturated Douglas fir forest in the Netherlands after decreased atmospheric nitrogen input.

International audienceThe MERLIN model was applied on the results of a field-scale manipulation experiment with decreased nitrogen (N) deposition in an N saturated forest ecosystem in the Netherlands. The aim was to investigate the mechanisms that could explain the observed rapid response of nitrate as a result of the decreased N input. Calibrating the model to pre-treatment data revealed that, despite the high atmospheric N input, the trees relied on N mineralised from refractory organic matter (ROM) for their growth. MERLIN could simulate only the fast response of nitrate leaching after decreased input if this ROM mineralisation rate was decreased strongly at the time of the manipulation experiment

Assessing the potential of co-composting rose waste as a sustainable waste management strategy:Nutrient availability and disease control

The current limited usage of rose waste makes rose cultivation far from a sustainable circular industry. Unfavorable properties of horticultural waste such as the high lignin content of stems and high polyphenol levels in both flowers and leaves makes it difficult to re-use. These traits hamper an effective composting process and so far little studies have focused on optimizing this process. The aim of this study was to investigate the potential of (co-)composting rose waste with other on-farm available green wastes (tomato and kalanchoe) or mature rose compost to obtain an improved compost with high fertilizing capacity. In a small-scale composting system the evolution of five mixtures was closely monitored in terms of their physico-chemical parameters. The in-vitro disease suppressive capacity of mature rose compost was assessed. All mixtures resulted in stable and mature compost after six months showing industry standard suitable macro- and micro-nutrient concentrations. The matured compost showed a C/N below 10, a strong decrease in polyphenols of ≥70% and a good fertilizing capacity with an increase in cation exchange capacity since the start of ≥100%. These results demonstrate that the ligneous character of rose waste is not preventing an effective composting process. However, an increased duration of the maturation phase might be favored for optimal results. The addition of mature compost accelerated the composting process as shown by significantly increases in OM degradation rates. For the first time a high disease suppressive capacity against several common rose pathogens was shown for mature rose compost. Overall, this study showed the potential of (co-)composting rose waste as sustainable waste management strategy to further improve the circular economy waste-based objectives of the horticultural sector.</p

Effects of experimental increase of temperature and drought on heathland vegetation

Effects of simulated environmental changes on heathland vegeta-tion were investigated in Oldebroek, the Netherlands. As response to strong dis-turbance, decrease of the presence/coverage of lichen species was observed; bryophytes have shown various reactions. In the drought plots the normally pre-dominant species are decreasing, while others reach their maximum coverage here

Towards reduced uncertainty in catchment nitrogen modelling: quantifying the effect of field observation uncertainty on model calibration

International audienceThe value of nitrogen (N) field measurements for the calibration of parameters of the INCA nitrogen in catchment model is explored and quantified. A virtual catchment was designed by running INCA with a known set of parameters, and field "measurements" were selected from the model run output. Then, using these measurements and the Shuffled Complex Evolution Metropolis algorithm (SCEM-UA), four of the INCA model parameters describing N transformations in the soil were optimised, while the measurement uncertainty was increased in subsequent steps. Considering measurement uncertainty typical for N field studies, none of the synthesised datasets contained sufficient information to identify the model parameters with a reasonable degree of confidence. Parameter equifinality occurred, leading to considerable uncertainty in model parameter values and in modelled N concentrations and fluxes. Fortunately, combining the datasets in a multi-objective calibration was found to be effective in dealing with these equifinality problems. With the right choice of calibration measurements, multi-objective calibrations resulted in lower parameter uncertainty. The methodology applied in this study, using a virtual catchment free of model errors, is proposed as a useful tool foregoing the application of a N model or the design of a N monitoring program. For an already gauged catchment, a virtual study can provide a point of reference for the minimum uncertainty associated with a model application. When setting up a monitoring program, it can help to decide what and when to measure. Numerical experiments indicate that for a forested, N-saturated catchment, a fortnightly sampling of NO3 and NH4 concentrations in stream water may be the most cost-effective monitoring strategy. Keywords: INCA, nitrogen model, parameter uncertainty, multi-objective calibration, virtual catchment, experimental desig

Long-term temporal patterns in ecosystem carbon flux components and overall balance in a heathland ecosystem

Terrestrial ecosystems have strong feedback to atmospheric CO2 concentration and climate change. However, the long-term whole life cycle dynamics of ecosystem carbon (C) fluxes and overall balance in some ecosystem types, such as heathland ecosystems, have not been thoroughly explored. We studied the changes in ecosystem CO2 flux components and overall C balance over a full ecosystem lifecycle in stands of Calluna vulgaris (L.) Hull by using a chronosequence of 0, 12, 19 and 28 years after vegetation cutting. Overall, the ecosystem C balance was highly nonlinear over time and exhibited a sinusoidal-like curvature of C sink/source change over the three-decade timescale. After cutting, plant-related C flux components of gross photosynthesis (PG), aboveground autotrophic respiration (Raa) and belowground autotrophic respiration (Rba) were higher at the young age (12 years) than at middle (19 years) and old (28 years) ages. The young ecosystem was a C sink (12 years: −0.374 kg C m−2 year−1) while it became a C source with aging (19 years: 0.218 kg C m−2 year−1) and when dying (28 years: 0.089 kg C m−2 year−1). The post-cutting C compensation point was observed after four years, while the cumulative C loss in the period after cutting had been compensated by an equal amount of C uptake after seven years. Annual ecosystem C payback from the ecosystem to the atmosphere started after 16 years. This information may be used directly for optimizing vegetation management practices for maximal ecosystem C uptake capacity. Our study highlights that whole life cycle observational data of changes in C fluxes and balance in ecosystems are important and the ecosystem model needs to take the successional stage and vegetation age into account when projecting component C fluxes, ecosystem C balance, and overall feedback to climate change

Dynamic nitrogen deposition thresholds during forest stand development in a Douglas fir forest analysed with two nitrogen models SMART2 and MERLIN

In contrast to the classical critical load (CL) concept, based on long-term steady-state conditions, a dynamic deposition threshold (DDT) is introduced. This DDT takes into account all relevant dynamic aspects of vegetation development/forest growth, mineralisation, immobilisation and denitrification, depending on the successional stage of the forest. DDT values for nitrogen were determined for a Douglas fir rotation by two process-based nitrogen models SMART2 and MERLIN using three different criteria for critical nitrogen leaching. During most of the rotation time, the predicted DDT values were higher than the corresponding traditional CL. SMART2 and MERLIN predicted a maximum DDT of 4.9 and 4.6 kmol N per ha per year (69 and 64 kg N per ha per year, respectively), when accepting a critical N leaching level of 1.73 kmol N per ha per year related to impacts on ground water quality. This is due mainly to relatively high tree uptake during the first 50 years of a forest rotation, compared to a long-term estim