Distinct latitudinal community patterns of Arctic marine vertebrates along the East Greenlandic coast detected by environmental DNA

Aim: Greenland is one of the places on Earth where the effects of climate change are most evident. The retreat of sea ice has made East Greenland more accessible for longer periods during the year. East Greenland fjords have been notoriously difficult to study due to their remoteness, dense sea ice conditions and lack of infrastructure. As a result, biological monitoring across latitudinal gradients is scarce in East Greenland and relies on sporadic research cruises and trawl data from commercial vessels. We here aim to investigate the transition in fish and marine mammal communities from South to Northeast Greenland using environmental DNA (eDNA). Location: South to Northeast Greenland. Methods: We investigated the transition in fish and marine mammal communities from South to Northeast Greenland using eDNA metabarcoding of seawater samples. We included both surface and mesopelagic samples, collected over approximately 2400 km waterway distance, by sampling from Cape Farewell to Ella Island in August 2021. Results: We demonstrate a clear transition in biological communities from south to northeast, with detected fish and mammal species matching known distributions. Samples from the southern areas were dominated by capelin (Mallotus villosus) and redfish (Sebastes), whereas northeastern samples were dominated by polar cod (Boreogadus saida), sculpins (Myoxocephalus) and ringed seal (Pusa hispida). We provide newly generated 12S rRNA barcodes from 87 fish species, bringing the public DNA database closer to full taxonomic coverage for Greenlandic fish species for this locus. Main Conclusions: Our results demonstrate that eDNA sampling can detect latitudinal shifts in marine biological communities of the Arctic region, which can supplement traditional fish surveys in understanding species distributions and community compositions of marine vertebrates. Importantly, sampling of eDNA can be a feasible approach for detecting northward range expansions in remote areas as climate change progresses

Research data management challenges in citizen science projects and recommendations for library support services. A scoping review and case study.

Citizen science (CS) projects are part of a new era of data aggregation and harmonisation that facilitates interconnections between different datasets. Increasing the value and reuse of CS data has received growing attention with the appearance of the FAIR principles and systematic research data management (RDM) practises, which are often promoted by university libraries. However, RDM initiatives in CS appear diversified and if CS have special needs in terms of RDM is unclear. Therefore, the aim of this article is firstly to identify RDM challenges for CS projects and secondly, to discuss how university libraries may support any such challenges. A scoping review and a case study of Danish CS projects were performed to identify RDM challenges. 48 articles were selected for data extraction. Four academic project leaders were interviewed about RDM practices in their CS projects. Challenges and recommendations identified in the review and case study are often not specific for CS. However, finding CS data, engaging specific populations, attributing volunteers and handling sensitive data including health data are some of the challenges requiring special attention by CS project managers. Scientific requirements or national practices do not always encompass the nature of CS projects. Based on the identified challenges, it is recommended that university libraries focus their services on 1) identifying legal and ethical issues that the project managers should be aware of in their projects, 2) elaborating these issues in a Terms of Participation that also specifies data handling and sharing to the citizen scientist, and 3) motivating the project manager to good data handling practises. Adhering to the FAIR principles and good RDM practices in CS projects will continuously secure contextualisation and data quality. High data quality increases the value and reuse of the data and, therefore, the empowerment of the citizen scientists

Environmental DNA metabarcoding reveals seasonal and spatial variation in the vertebrate fauna of Ilulissat Icefjord, Greenland

Ilulissat Icefjord in Greenland is experiencing the effects of climate change, with the Sermeq Kujalleq glacier being one of the fastest-moving and most productive ice streams in Greenland. This is likely affecting the distribution of species in the fjord, including those important to local fisheries. Due to heavy ice conditions, few studies on environmental and ecological conditions exist from the fjord. However, new techniques such as environmental DNA (eDNA) meta barcoding now allow deeper insight into the fjord system. Here, we combine local ecological knowledge with data on hydrographic conditions, stable isotopes (δ18O), and eDNA metabarcoding to investigate the spatial and seasonal distribution of marine fish and mammals inside Ilulissat Icefjord. Our eDNA results support local observations that Arctic char migrate to the southern fjord during summer, harp seals forage in large herds in the fjord system, polar cod is the dominant prey fish in the area, and Greenland shark likely does not reside in the fjord system. Lower predation pressure in the Icefjord, due to the absence of Greenland shark and polar bears as well as limited fishing/hunting, is presumably one of the reasons why ringed seals and Greenland halibut are larger in the Icefjord. Furthermore, our results indicate that in summer, the southern branch of the fjord system has a more diverse community of vertebrates and different water masses than the northern branch and main fjord, indicating a time lag between inflows to the different branches of the fjord system. Our approach highlights the value of combining local ecological knowledge with scientific research and represents a potential starting point for monitoring biological responses in Ilulissat Icefjord associated with climate-induced changes

Induced activation in accelerator components

The residual activity induced in particle accelerators is a serious issue from the point of view of radiation safety as the long-lived radionuclides produced by fast or moderated neutrons and impact protons cause problems of radiation exposure for staff involved in the maintenance work and when decommissioning the facility. This paper presents activation studies of the magnets and collimators in the High Energy Beam Transport line of the European Spallation Source due to the backscattered neutrons from the target and also due to the direct proton interactions and their secondaries. An estimate of the radionuclide inventory and induced activation are predicted using the GEANT4 code

The Modular X- and Gamma-Ray Sensor (MXGS) of the ASIM Payload on the International Space Station

The Modular X- and Gamma-ray Sensor (MXGS) is an imaging and spectral X- and Gamma-ray instrument mounted on the starboard side of the Columbus module on the International Space Station. Together with the Modular Multi-Spectral Imaging Assembly (MMIA) (Chanrion et al. this issue) MXGS constitutes the instruments of the Atmosphere-Space Interactions Monitor (ASIM) (Neubert et al. this issue). The main objectives of MXGS are to image and measure the spectrum of X- and γ-rays from lightning discharges, known as Terrestrial Gamma-ray Flashes (TGFs), and for MMIA to image and perform high speed photometry of Transient Luminous Events (TLEs) and lightning discharges. With these two instruments specifically designed to explore the relation between electrical discharges, TLEs and TGFs, ASIM is the first mission of its kind. With an imaging system and a large detector area MXGS will, for the first time, allow estimation of the location of the source region and characterization of the energy spectrum of individual events. The sensors have fast readout electronics to minimize pileup effects, giving high time resolution of photon detection for comparison with measurements on μs-time scales of lightning processes measured by the MMIA and other sensors in space or on the ground. The detectors cover the large energy range of the relevant photon energies. In this paper we describe the scientific objectives, design, performance, imaging capabilities and operational modes of the MXGS instrument

Temporal development of brominated flame retardants in peregrine falcon (Falco peregrinus) eggs from South Greenland (1986-2003)

A time trend between 1986 and 2003 was found for brominated flame retardants in peregrine falcon eggs from South Greenland, with significantly increasing concentrations of the polybrominated diphenyl ethers (PBDEs) 99, 100, 153, 154, and 209. For BDE-99 and -100, the concentration increased approximately 10% per year. The concentrations of PBDEs were among the highest detected in wildlife so far and ranged from 300 to 12 900 ng/g lipid weight (lw) for ¿PBDE. While tetrabromobisphenol A (TBBPA) was below the limit of detection in all eggs, hexabromocyclododecane (HBCD), dimethyl-TBBPA, and brominated biphenyl BB-153 were detected in a majority of eggs, with median concentrations of 2.4, 230, and 550 ng/g lw, respectively. Analyses of eggs of the same bird showed no significant intra-clutch variation for PBDEs, BB-153, and HBCD but larger variations for dimethyl-TBBPA. Inter-clutch variations with increasing time trends exist for the BDEs 99, 100, 153, 154, and 209, while a decreasing contamination with the BDEs 183, 49, 47, 66 and 153 was indicated in a subset of eggs