Yersinia ruckeri isolates recovered from diseased Atlantic Salmon (Salmo salar) in Scotland are more diverse than those from Rainbow Trout (Oncorhynchus mykiss) and represent distinct subpopulations

Yersinia ruckeri is the etiological agent of enteric redmouth (ERM) disease of farmed salmonids. Enteric redmouth disease is traditionally associated with rainbow trout (Oncorhynchus mykiss, Walbaum), but its incidence in Atlantic salmon (Salmo salar) is increasing. Yersinia ruckeri isolates recovered from diseased Atlantic salmon have been poorly characterized, and very little is known about the relationship of the isolates associated with these two species. Phenotypic approaches were used to characterize 109 Y. ruckeri isolates recovered over a 14-year period from infected Atlantic salmon in Scotland; 26 isolates from infected rainbow trout were also characterized. Biotyping, serotyping, and comparison of outer membrane protein profiles identified 19 Y. ruckeri clones associated with Atlantic salmon but only five associated with rainbow trout; none of the Atlantic salmon clones occurred in rainbow trout and vice versa. These findings suggest that distinct subpopulations of Y. ruckeri are associated with each species. A new O serotype (designated O8) was identified in 56 biotype 1 Atlantic salmon isolates and was the most common serotype identified from 2006 to 2011 and in 2014, suggesting an increased prevalence during the time period sampled. Rainbow trout isolates were represented almost exclusively by the same biotype 2, serotype O1 clone that has been responsible for the majority of ERM outbreaks in this species within the United Kingdom since the 1980s. However, the identification of two biotype 2, serotype O8 isolates in rainbow trout suggests that vaccines containing serotypes O1 and O8 should be evaluated in both rainbow trout and Atlantic salmon for application in Scotland

Galactic Archaeology with TESS: Prospects for Testing the Star Formation History in the Solar Neighbourhood

A period of quenching between the formation of the thick and thin disks of the Milky Way has been recently proposed to explain the observed age-[{\alpha}/Fe] distribution of stars in the solar neighbourhood. However, robust constraints on stellar ages are currently available for only a limited number of stars. The all-sky survey TESS (Transiting Exoplanet Survey Satellite) will observe the brightest stars in the sky and thus can be used to investigate the age distributions of stars in these components of the Galaxy via asteroseismology, where previously this has been difficult using other techniques. The aim of this preliminary study was to determine whether TESS will be able to provide evidence for quenching periods during the star formation history of the Milky Way. Using a population synthesis code, we produced populations based on various stellar formation history models and limited the analysis to red-giant-branch stars. We investigated the mass-Galactic-disk-height distributions, where stellar mass was used as an age proxy, to test for whether periods of quenching can be observed by TESS. We found that even with the addition of 15% noise to the inferred masses, it will be possible for TESS to find evidence for/against quenching periods suggested in the literature (e.g. between 7 and 9 Gyr ago), therefore providing stringent constraints on the formation and evolution of the Milky Way.Comment: 4 pages, 3 figures, proceedings of "Seismology of the Sun and the Distant Stars 2016", Mario J. P. F. G. Monteiro, Margarida S. Cunha, Joao Miguel T. Ferreira editor

Stemming the tide of light pollution encroaching into Marine Protected Areas

Open Access journalMany marine ecosystems are shaped by regimes of natural light guiding the behaviour of their constituent species. As evidenced from terrestrial systems, the global introduction of nighttime lighting is likely influencing these behaviours, restructuring marine ecosystems, and compromising the services they provide. Yet the extent to which marine habitats are exposed to artificial light at night is unknown. We quantified nightime artificial light across the world's network of Marine Protected Areas (MPAs). Artificial light is widespread and increasing in a large percentage of MPAs. While increases are more common among MPAs associated with human activity, artificial light is encroaching into a large proportion of even those marine habitats protected with the strongest legislative designations. Given the current lack of statutory tools, we propose that allocating ‘marine dark sky park’ status to MPAs will help incentivize responsible authorities to hold back the advance of artificial light.European Research Council under the European Union’s Seventh Framework Programme (FP7/2007-2013

Night-time lighting alters the composition of marine epifaunal communities

Marine benthic communities face multiple anthropogenic pressures that compromise the future of some of the most biodiverse and functionally important ecosystems in the world. Yet one of the pressures these ecosystems face, night-time lighting, remains unstudied. Light is an important cue in guiding the settlement of invertebrate larvae, and altering natural regimes of nocturnal illumination could modify patterns of recruitment among sessile epifauna. We present the first evidence of night-time lighting changing the composition of temperate epifaunal marine invertebrate communities. Illuminating settlement surfaces with white light-emitting diode lighting at night, to levels experienced by these communities locally, both inhibited and encouraged the colonization of 39% of the taxa analysed, including three sessile and two mobile species. Our results indicate that ecological light pollution from coastal development, shipping and offshore infrastructure could be changing the composition of marine epifaunal communities.European Research Council under the European Union's Seventh Framework programme (FP7/2007-2013

BIVALVE AQUACULTURE AND EXOTIC SPECIES: A REVIEW OF ECOLOGICAL CONSIDERATIONS AND MANAGEMENT ISSUES

Peer-reviewed.Bivalves have been grown and transported for culture for hundreds of years and the introduction of some species outside of their native range for aquaculture has been suggested to be one of the greatest modes of introduction of exotic marine species. However, there has yet to be a thorough assessment of the importance of aquaculture and bivalve culture in particular, to the introduction and spread of exotic species. This paper reviews some of the environmental and ecological implications of the relationship between bivalve aquaculture and the introduction and spread of exotic species, management implications and mitigation strategies. Two broad classes of introductions of exotic species may result from activities associated with bivalve aquaculture. First, the intentional introduction of exotic species into an area for aquaculture purposes, i.e. the ‘‘target’’ species. These are typically foundation or engineering species and may have a considerable influence on receiving ecosystems. Second, the introduction of species that are either associated with introduced bivalves or facilitated by aquaculture activities (i.e. structures or husbandry practices). These may include both ‘‘hitchhiking’’ species (organisms that grow in association with or may be transferred with cultured bivalves) and disease causing organisms.Management options should include the use of risk assessments prior to transfers and quarantines. Various types of mitigation for exotic species have been evaluated but are generally not very successful. Because the risk of exotic species to ecosystems and the bivalve farming industry itself may be great, effort should be directed to better predict and halt introductions of potentially harmful species

Artificial Light at Night Causes Top‐down and Bottom‐up Trophic Effects on Invertebrate Populations

This is the author accepted manuscript. The final version is available from Wiley via the DOI in this record.1. Globally, many ecosystems are exposed to artificial light at night. Nighttime lighting has direct biological impacts on species at all trophic levels. However, the effects of artificial light on biotic interactions remain, for the most part, to be determined. 2. We exposed experimental mesocosms containing combinations of grassland plants and invertebrate herbivores and predators to illumination at night over a three-year period to simulate conditions under different common forms of street lighting. 3. We demonstrate both top-down (predation controlled) and bottom-up (resource controlled) impacts of artificial light at night in grassland communities. The impacts on invertebrate herbivore abundance were wavelength dependent and mediated via other trophic levels. 4. White LED lighting decreased the abundance of a generalist herbivore mollusc by 55% in the presence of a visual predator, but not in its absence, while monochromatic amber light (with a peak wavelength similar to low pressure sodium lighting) decreased abundance of a specialist herbivore aphid (by 17%) by reducing the cover and flower abundance of its main food plant in the system. Artificial white light also significantly increased the food plant’s foliar carbon to nitrogen ratio. 5. We conclude that exposure to artificial light at night can trigger ecological effects spanning trophic levels, and that the nature of such impacts depends on the wavelengths emitted by the lighting technology employed. 6. Policy implications. Our results confirm that artificial light at night, at illuminance levels similar to roadside vegetation, can have population effects mediated by both top-down and bottom-up effects on ecosystems. Given the increasing ubiquity of light pollution at night, these impacts may be widespread in the environment. These results underline the importance of minimising ecosystem disruption by reducing light pollution in natural and semi-natural ecosystems.The research leading to this paper was funded by the European Research Council under the European Union’s Seventh Framework programme (FP7/2007-2013)/ERC grant agreement no. 268504 to KJG

Tuning the interactions between electron spins in fullerene-based triad systems

A series of six fullerene-linker-fullerene triads have been prepared by the stepwise addition of the fullerene cages to bridging moieties thus allowing the systematic variation of fullerene cage (C60 or C70) and linker (oxalate or terephthalate) and enabling precise control over the inter-fullerene separation. The fullerene triads exhibit good solubility in common organic solvents, have linear geometries and are diastereomerically pure. Cyclic voltammetric measurements demonstrate the excellent electron accepting capacity of all triads, with up to 6 electrons taken up per molecule in the potential range between -2.3 and 0.2 V (vs. Fc+/Fc). No significant electronic interactions between fullerene cages are observed in the ground state indicating that the individual properties of each C60 or C70 cage are retained within the triads. The electron-electron interactions in the electrochemically generated dianions of these triads, with one electron per fullerene cage were studied by EPR spectroscopy. The nature of electron-electron coupling observed at 77 K can be described as an equilibrium between a doublet and triplet state biradical which depends on the interfullerene spacing. The shorter oxalate-bridged triads exhibit stronger spin-spin coupling with triplet character, while in the longer terephthalate-bridged triads the intramolecular spin-spin coupling is significantly reduced