First Accuracy Evaluation of NIST-F2

We report the first accuracy evaluation of NIST-F2, a second-generation laser-cooled Cesium fountain primary standard developed at the National Institute of Standards and Technology (NIST) with a cryogenic (Liquid Nitrogen) microwave cavity and flight region. The 80 K atom interrogation environment reduces the uncertainty due to the Blackbody Radiation (BBR) shift by more than a factor of 50. Also, the Ramsey microwave cavity exhibits a high Q (>50,000) at this low temperature, resulting in a reduced distributed cavity phase shift. NIST-F2 has undergone many tests and improvements since we first began operation in 2008. In the last few years NIST-F2 has been compared against a NIST maser time scale and NIST-F1 (the US primary frequency standard) as part of in-house accuracy evaluations. We report the results of nine in-house comparisons since 2010 with a focus on the most recent accuracy evaluation. This paper discusses the design of the physics package, the laser and optics systems, and the accuracy evaluation methods. The Type B fractional uncertainty of NIST-F2 is shown to be 0.11 × 10-15 and is dominated by microwave amplitude dependent effects. The most recent evaluation (August 2013) had a statistical (Type A) fractional uncertainty of 0.44 × 10-15

Low-concentration, continuous brachial plexus block in the management of Purple Glove Syndrome: a case report

<p>Abstract</p> <p>Introduction</p> <p>Purple Glove Syndrome is a devastating complication of intravenous phenytoin administration. Adequate analgesia and preservation of limb movement for physiotherapy are the two essential components of management.</p> <p>Case presentation</p> <p>A 26-year-old Tamil woman from India developed Purple Glove Syndrome after intravenous administration of phenytoin. She was managed conservatively by limb elevation, physiotherapy and oral antibiotics. A 20G intravenous cannula was inserted into the sheath of her brachial plexus and a continuous infusion of bupivacaine at a low concentration (0.1%) with fentanyl (2 μg/ml) at a rate of 1 to 2 ml/hr was given. She had adequate analgesia with preserved motor function which helped in physiotherapy and functional recovery of the hand in a month.</p> <p>Conclusion</p> <p>A continuous blockade of the brachial plexus with a low concentration of bupivacaine and fentanyl helps to alleviate the vasospasm and the pain while preserving the motor function for the patient to perform active movements of the finger and hand.</p

The mesospheric inversion layer and sprites

The vertical structure of temperature observed by SABER (Sounding of Atmosphere using Broadband Emission Radiometry) aboard TIMED (Thermosphere, Ionosphere, Mesosphere Energetics and Dynamics) and sprites observations made during the Eurosprite 2003 to 2007 observational campaign were analyzed. Sprite observations were made at two locations in France, namely Puy de Dome in the French Massif Central and at the Pic du Midi in the French Pyrenees. It is observed that the vertical structure of temperature shows evidence for a Mesospheric Inversion Layer (MIL) on those days on which sprites were observed. A few events are also reported in which sprites were not recorded, although there is evidence of a MIL in the vertical structure of the temperature. It is proposed that breaking gravity waves produced by convective thunderstorms facilitate the production of (a) sprites by modulating the neutral air-density and (b) MILs via the deposition of energy. The same proposition has been used to explain observations of lightings as well as both MILs and lightning arising out of deep convections.Comment: 34 pages, 5figures. Accepted in Journal of Geophysical Research, US

Genetic determinants of telomere length from 109,122 ancestrally diverse whole-genome sequences in TOPMed

Genetic studies on telomere length are important for understanding age-related diseases. Prior GWAS for leukocyte TL have been limited to European and Asian populations. Here, we report the first sequencing-based association study for TL across ancestrally-diverse individuals (European, African, Asian and Hispanic/Latino) from the NHLBI Trans-Omics for Precision Medicine (TOPMed) program. We used whole genome sequencing (WGS) of whole blood for variant genotype calling and the bioinformatic estimation of telomere length in n=109,122 individuals. We identified 59 sentinel variants (p-value OBFC1indicated the independent signals colocalized with cell-type specific eQTLs for OBFC1 (STN1). Using a multi-variant gene-based approach, we identified two genes newly implicated in telomere length, DCLRE1B (SNM1B) and PARN. In PheWAS, we demonstrated our TL polygenic trait scores (PTS) were associated with increased risk of cancer-related phenotypes

Anaerobiosis revisited: growth of Saccharomyces cerevisiae under extremely low oxygen availability

The budding yeast Saccharomyces cerevisiae plays an important role in biotechnological applications, ranging from fuel ethanol to recombinant protein production. It is also a model organism for studies on cell physiology and genetic regulation. Its ability to grow under anaerobic conditions is of interest in many industrial applications. Unlike industrial bioreactors with their low surface area relative to volume, ensuring a complete anaerobic atmosphere during microbial cultivations in the laboratory is rather difficult. Tiny amounts of O2 that enter the system can vastly influence product yields and microbial physiology. A common procedure in the laboratory is to sparge the culture vessel with ultrapure N2 gas; together with the use of butyl rubber stoppers and norprene tubing, O2 diffusion into the system can be strongly minimized. With insights from some studies conducted in our laboratory, we explore the question ‘how anaerobic is anaerobiosis?’. We briefly discuss the role of O2 in non-respiratory pathways in S. cerevisiae and provide a systematic survey of the attempts made thus far to cultivate yeast under anaerobic conditions. We conclude that very few data exist on the physiology of S. cerevisiae under anaerobiosis in the absence of the anaerobic growth factors ergosterol and unsaturated fatty acids. Anaerobicity should be treated as a relative condition since complete anaerobiosis is hardly achievable in the laboratory. Ideally, researchers should provide all the details of their anaerobic set-up, to ensure reproducibility of results among different laboratories. A correction to this article is available online at http://eprints.whiterose.ac.uk/131930/ https://doi.org/10.1007/s00253-018-9036-

SBML Level 3: an extensible format for the exchange and reuse of biological models

Systems biology has experienced dramatic growth in the number, size, and complexity of computational models. To reproduce simulation results and reuse models, researchers must exchange unambiguous model descriptions. We review the latest edition of the Systems Biology Markup Language (SBML), a format designed for this purpose. A community of modelers and software authors developed SBML Level 3 over the past decade. Its modular form consists of a core suited to representing reaction-based models and packages that extend the core with features suited to other model types including constraint-based models, reaction-diffusion models, logical network models, and rule-based models. The format leverages two decades of SBML and a rich software ecosystem that transformed how systems biologists build and interact with models. More recently, the rise of multiscale models of whole cells and organs, and new data sources such as single-cell measurements and live imaging, has precipitated new ways of integrating data with models. We provide our perspectives on the challenges presented by these developments and how SBML Level 3 provides the foundation needed to support this evolution

The absolute frequency of the ⁸⁷Sr optical clock transition

The absolute frequency of the 1S0-3P0 clock transition of 87Sr has been measured to be 429 228 004 229 873.65 (37) Hz using lattice-confined atoms, where the fractional uncertainty of 8.6x10-16 represents one of the most accurate measurements of an atomic transition frequency to date. After a detailed study of systematic effects, which reduced the total systematic uncertainty of the Sr lattice clock to 1.5x10-16, the clock frequency is measured against a hydrogen maser which is simultaneously calibrated to the US primary frequency standard, the NIST Cs fountain clock, NIST-F1. The comparison is made possible using a femtosecond laser based optical frequency comb to phase coherently connect the optical and microwave spectral regions and by a 3.5 km fiber transfer scheme to compare the remotely located clock signals